(Linear) (Six-base) MAPSORT of: KIEE2632.seq Check: 9690 from: 1 to: 6219 KIEE2632.orf With 182 enzymes: SgfI * August 8, 2016 10:02 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 3114 6219 Size: 3114 3105 AccI GT'mk_AC Cuts at: 0 4436 4988 6219 Size: 4436 552 1231 Fragments arranged by size: 4436 1231 552 Acc65I G'GTAC_C Cuts at: 0 1444 2153 6219 Size: 1444 709 4066 Fragments arranged by size: 4066 1444 709 AclI AA'CG_TT Cuts at: 0 2446 6219 Size: 2446 3773 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 104 397 1639 2087 3014 3869 4508 5427 Size: 104 293 1242 448 927 855 639 919 Cuts at: 5427 6219 Size: 792 Fragments arranged by size: 1242 927 919 855 792 639 448 293 104 AflII C'TTAA_G Cuts at: 0 783 6219 Size: 783 5436 AflIII A'CryG_T Cuts at: 0 3985 6219 Size: 3985 2234 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3122 5218 5879 6219 Size: 3122 2096 661 340 Fragments arranged by size: 3122 2096 661 340 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 3056 3088 5095 5127 6219 Size: 3056 32 2007 32 1092 Fragments arranged by size: 3056 2007 1092 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 142 453 821 2992 3183 5081 5295 5806 Size: 142 311 368 2171 191 1898 214 511 Cuts at: 5806 6219 Size: 413 Fragments arranged by size: 2171 1898 511 413 368 311 214 191 142 ApoI r'AATT_y Cuts at: 0 748 2170 2254 2339 2393 3813 6219 Size: 748 1422 84 85 54 1420 2406 Fragments arranged by size: 2406 1422 1420 748 85 84 54 AvaI C'yCGr_G Cuts at: 0 1686 5353 6219 Size: 1686 3667 866 Fragments arranged by size: 3667 1686 866 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 541 574 6219 Size: 541 33 5645 Fragments arranged by size: 5645 541 33 BamHI G'GATC_C Cuts at: 0 1081 6219 Size: 1081 5138 BanI G'GyrC_C Cuts at: 0 549 1222 1444 2153 6085 6219 Size: 549 673 222 709 3932 134 Fragments arranged by size: 3932 709 673 549 222 134 BanII G_rGCy'C Cuts at: 0 1301 1513 3476 4466 6219 Size: 1301 212 1963 990 1753 Fragments arranged by size: 1963 1753 1301 990 212 BbsI GAAGACnn'nnnn_ Cuts at: 0 139 2147 3802 4205 6219 Size: 139 2008 1655 403 2014 Fragments arranged by size: 2014 2008 1655 403 139 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 4548 4582 6219 Size: 4548 34 1637 Fragments arranged by size: 4548 1637 34 BciVI GTATCCnnnnn_n' Cuts at: 0 569 2515 6219 Size: 569 1946 3704 Fragments arranged by size: 3704 1946 569 BclI T'GATC_A Cuts at: 0 1980 2576 4218 5484 6219 Size: 1980 596 1642 1266 735 Fragments arranged by size: 1980 1642 1266 735 596 BfrBI ATG'CAT Cuts at: 0 1093 6219 Size: 1093 5126 BlpI GC'TnA_GC Cuts at: 0 3023 6219 Size: 3023 3196 Bme1580I G_kGCm'C Cuts at: 0 552 1586 2803 3993 6088 6219 Size: 552 1034 1217 1190 2095 131 Fragments arranged by size: 2095 1217 1190 1034 552 131 BmrI ACTGGGnnnn_n' Cuts at: 0 469 557 1508 3899 3950 4789 6219 Size: 469 88 951 2391 51 839 1430 Fragments arranged by size: 2391 1430 951 839 469 88 51 BmtI G_CTAG'C Cuts at: 0 3047 6219 Size: 3047 3172 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2312 3205 3535 5115 6219 Size: 2312 893 330 1580 1104 Fragments arranged by size: 2312 1580 1104 893 330 Bpu10I CC'TnA_GC Cuts at: 0 1591 6219 Size: 1591 4628 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 373 1167 3944 4679 5237 6219 Size: 373 794 2777 735 558 982 Fragments arranged by size: 2777 982 794 735 558 373 BsaI GGTCTCn'nnnn_ Cuts at: 0 1407 2669 3433 6219 Size: 1407 1262 764 2786 Fragments arranged by size: 2786 1407 1262 764 BsaHI Gr'CG_yC Cuts at: 0 4433 6219 Size: 4433 1786 BsaWI w'CCGG_w Cuts at: 0 1501 3799 6219 Size: 1501 2298 2420 Fragments arranged by size: 2420 2298 1501 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1530 1560 1789 1819 4115 4145 5095 5113 Size: 1530 30 229 30 2296 30 950 18 Cuts at: 5113 5125 5143 5643 5673 6219 Size: 12 18 500 30 546 Fragments arranged by size: 2296 1530 950 546 500 229 30 30 30 30 18 18 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 360 370 691 1791 1794 1797 2193 3232 Size: 360 10 321 1100 3 3 396 1039 Cuts at: 3232 3235 3238 3247 3250 3262 3289 3937 3940 Size: 3 3 9 3 12 27 648 3 Cuts at: 3940 4078 4081 4084 4087 4136 4243 4246 4525 Size: 138 3 3 3 49 107 3 279 Cuts at: 4525 5240 5645 5648 6219 Size: 715 405 3 571 Fragments arranged by size: 1100 1039 715 648 571 405 396 360 321 279 138 107 49 27 12 10 9 3 3 3 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 435 1814 2585 3050 4477 4593 5165 5413 Size: 435 1379 771 465 1427 116 572 248 Cuts at: 5413 5993 6089 6101 6219 Size: 580 96 12 118 Fragments arranged by size: 1427 1379 771 580 572 465 435 248 118 116 96 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 731 977 1596 2048 4190 4411 4751 5325 Size: 731 246 619 452 2142 221 340 574 Cuts at: 5325 5849 6219 Size: 524 370 Fragments arranged by size: 2142 731 619 574 524 452 370 340 246 221 BsiEI CG_ry'CG Cuts at: 0 1033 6219 Size: 1033 5186 BsiHKAI G_wGCw'C Cuts at: 0 5026 6219 Size: 5026 1193 BsiWI C'GTAC_G Cuts at: 0 4830 6219 Size: 4830 1389 BsmI GAATG_Cn' Cuts at: 0 568 808 898 923 1093 1095 3138 3574 Size: 568 240 90 25 170 2 2043 436 Cuts at: 3574 3651 6219 Size: 77 2568 Fragments arranged by size: 2568 2043 568 436 240 170 90 77 25 2 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2701 3445 5350 5795 6219 Size: 2701 744 1905 445 424 Fragments arranged by size: 2701 1905 744 445 424 Bsp1286I G_dGCh'C Cuts at: 0 552 1301 1513 1586 2803 3476 3993 4466 Size: 552 749 212 73 1217 673 517 473 Cuts at: 4466 5026 6088 6219 Size: 560 1062 131 Fragments arranged by size: 1217 1062 749 673 560 552 517 473 212 131 73 BspHI T'CATG_A Cuts at: 0 981 6219 Size: 981 5238 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 3114 3846 5242 6219 Size: 3114 732 1396 977 Fragments arranged by size: 3114 1396 977 732 BsrBI CCG'CTC Cuts at: 0 3027 6219 Size: 3027 3192 BsrDI GCAATG_nn' Cuts at: 0 315 2810 3633 5491 6219 Size: 315 2495 823 1858 728 Fragments arranged by size: 2495 1858 823 728 315 BsrFI r'CCGG_y Cuts at: 0 2150 3152 6219 Size: 2150 1002 3067 Fragments arranged by size: 3067 2150 1002 BsrGI T'GTAC_A Cuts at: 0 3818 6140 6219 Size: 3818 2322 79 Fragments arranged by size: 3818 2322 79 BssSI C'ACGA_G Cuts at: 0 1352 6219 Size: 1352 4867 BstAPI GCAn_nnn'nTGC Cuts at: 0 453 803 812 1890 5536 6111 6219 Size: 453 350 9 1078 3646 575 108 Fragments arranged by size: 3646 1078 575 453 350 108 9 BstEII G'GTnAC_C Cuts at: 0 1109 6219 Size: 1109 5110 BstXI CCAn_nnnn'nTGG Cuts at: 0 1762 5340 6219 Size: 1762 3578 879 Fragments arranged by size: 3578 1762 879 BstYI r'GATC_y Cuts at: 0 1081 4140 6219 Size: 1081 3059 2079 Fragments arranged by size: 3059 2079 1081 Bsu36I CC'TnA_GG Cuts at: 0 4049 6219 Size: 4049 2170 BtgI C'CryG_G Cuts at: 0 117 1736 3839 4187 4601 6126 6219 Size: 117 1619 2103 348 414 1525 93 Fragments arranged by size: 2103 1619 1525 414 348 117 93 BtsI GCAGTG_nn' Cuts at: 0 799 822 1584 2993 3210 3432 6219 Size: 799 23 762 1409 217 222 2787 Fragments arranged by size: 2787 1409 799 762 222 217 23 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2292 2327 6219 Size: 2292 35 3892 Fragments arranged by size: 3892 2292 35 DraI TTT'AAA Cuts at: 0 1842 3807 6219 Size: 1842 1965 2412 Fragments arranged by size: 2412 1965 1842 DraIII CAC_nnn'GTG Cuts at: 0 1357 4018 6219 Size: 1357 2661 2201 Fragments arranged by size: 2661 2201 1357 DrdI GACnn_nn'nnGTC Cuts at: 0 4432 6219 Size: 4432 1787 EaeI y'GGCC_r Cuts at: 0 1535 2148 3154 3742 4821 6219 Size: 1535 613 1006 588 1079 1398 Fragments arranged by size: 1535 1398 1079 1006 613 588 EarI CTCTTCn'nnn_ Cuts at: 0 80 2063 2434 2640 2713 2993 3209 3239 Size: 80 1983 371 206 73 280 216 30 Cuts at: 3239 3251 3257 3266 3278 3488 3608 4049 4055 Size: 12 6 9 12 210 120 441 6 Cuts at: 4055 4073 4082 4091 4220 4232 4241 4319 5100 Size: 18 9 9 129 12 9 78 781 Cuts at: 5100 6219 Size: 1119 Fragments arranged by size: 1983 1119 781 441 371 280 216 210 206 129 120 80 78 73 30 18 12 12 12 9 9 9 9 6 6 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 104 397 1639 2087 2312 3014 3205 3535 Size: 104 293 1242 448 225 702 191 330 Cuts at: 3535 3869 4508 5115 5427 6219 Size: 334 639 607 312 792 Fragments arranged by size: 1242 792 702 639 607 448 334 330 312 293 225 191 104 EcoO109I rG'GnC_Cy Cuts at: 0 77 216 1931 4482 5611 5860 6219 Size: 77 139 1715 2551 1129 249 359 Fragments arranged by size: 2551 1715 1129 359 249 139 77 EcoRI G'AATT_C Cuts at: 0 2254 6219 Size: 2254 3965 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1644 1971 6219 Size: 1644 327 4248 Fragments arranged by size: 4248 1644 327 HaeII r_GCGC'y Cuts at: 0 237 5838 5923 6219 Size: 237 5601 85 296 Fragments arranged by size: 5601 296 237 85 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1066 1098 2600 2632 2852 2884 4942 4974 Size: 1066 32 1502 32 220 32 2058 32 Cuts at: 4974 5063 5095 5643 5675 6219 Size: 89 32 548 32 544 Fragments arranged by size: 2058 1502 1066 548 544 220 89 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1281 3080 4437 4842 5468 5937 6219 Size: 1281 1799 1357 405 626 469 282 Fragments arranged by size: 1799 1357 1281 626 469 405 282 HindIII A'AGCT_T Cuts at: 0 2239 2539 4341 6219 Size: 2239 300 1802 1878 Fragments arranged by size: 2239 1878 1802 300 HpaI GTT'AAC Cuts at: 0 1281 5468 6219 Size: 1281 4187 751 Fragments arranged by size: 4187 1281 751 KpnI G_GTAC'C Cuts at: 0 1448 2157 6219 Size: 1448 709 4062 Fragments arranged by size: 4062 1448 709 MfeI C'AATT_G Cuts at: 0 341 1847 6219 Size: 341 1506 4372 Fragments arranged by size: 4372 1506 341 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 104 2499 4760 5201 5254 6219 Size: 104 2395 2261 441 53 965 Fragments arranged by size: 2395 2261 965 441 104 53 MscI TGG'CCA Cuts at: 0 3744 4823 6219 Size: 3744 1079 1396 Fragments arranged by size: 3744 1396 1079 MslI CAynn'nnrTG Cuts at: 0 2750 4909 5770 5896 5902 5953 6178 6219 Size: 2750 2159 861 126 6 51 225 41 Fragments arranged by size: 2750 2159 861 225 126 51 41 6 MspA1I CmG'CkG Cuts at: 0 150 450 1617 3180 3513 5108 5153 5186 Size: 150 300 1167 1563 333 1595 45 33 Cuts at: 5186 5295 5340 5512 5806 6219 Size: 109 45 172 294 413 Fragments arranged by size: 1595 1563 1167 413 333 300 294 172 150 109 45 45 33 NaeI GCC'GGC Cuts at: 0 3154 6219 Size: 3154 3065 NcoI C'CATG_G Cuts at: 0 117 3839 4601 6219 Size: 117 3722 762 1618 Fragments arranged by size: 3722 1618 762 117 NdeI CA'TA_TG Cuts at: 0 887 6096 6219 Size: 887 5209 123 Fragments arranged by size: 5209 887 123 NgoMIV G'CCGG_C Cuts at: 0 3152 6219 Size: 3152 3067 NheI G'CTAG_C Cuts at: 0 3043 6219 Size: 3043 3176 NsiI A_TGCA'T Cuts at: 0 1095 6219 Size: 1095 5124 NspI r_CATG'y Cuts at: 0 815 2799 3989 5202 5991 6183 6219 Size: 815 1984 1190 1213 789 192 36 Fragments arranged by size: 1984 1213 1190 815 789 192 36 PasI CC'CwG_GG Cuts at: 0 229 5605 6219 Size: 229 5376 614 Fragments arranged by size: 5376 614 229 PciI A'CATG_T Cuts at: 0 3985 6219 Size: 3985 2234 PflMI CCAn_nnn'nTGG Cuts at: 0 123 5295 6219 Size: 123 5172 924 Fragments arranged by size: 5172 924 123 PfoI T'CCnGG_A Cuts at: 0 2432 6219 Size: 2432 3787 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1500 1532 5095 5127 6219 Size: 1500 32 3563 32 1092 Fragments arranged by size: 3563 1500 1092 32 32 PpuMI rG'GwC_Cy Cuts at: 0 216 1931 4482 5611 5860 6219 Size: 216 1715 2551 1129 249 359 Fragments arranged by size: 2551 1715 1129 359 249 216 PshAI GACnn'nnGTC Cuts at: 0 1135 4739 6219 Size: 1135 3604 1480 Fragments arranged by size: 3604 1480 1135 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 5998 6030 6219 Size: 5998 32 189 Fragments arranged by size: 5998 189 32 PstI C_TGCA'G Cuts at: 0 952 2828 2988 4925 5197 5207 5239 5462 Size: 952 1876 160 1937 272 10 32 223 Cuts at: 5462 6219 Size: 757 Fragments arranged by size: 1937 1876 952 757 272 223 160 32 10 PvuII CAG'CTG Cuts at: 0 150 1617 3180 3513 5108 5153 5295 5340 Size: 150 1467 1563 333 1595 45 142 45 Cuts at: 5340 6219 Size: 879 Fragments arranged by size: 1595 1563 1467 879 333 150 142 45 45 RsrII CG'GwC_CG Cuts at: 0 505 6219 Size: 505 5714 SalI G'TCGA_C Cuts at: 0 4435 6219 Size: 4435 1784 SanDI GG'GwC_CC Cuts at: 0 1931 4482 5611 6219 Size: 1931 2551 1129 608 Fragments arranged by size: 2551 1931 1129 608 SapI GCTCTTCn'nnn_ Cuts at: 0 2434 2713 2993 4241 4319 5100 6219 Size: 2434 279 280 1248 78 781 1119 Fragments arranged by size: 2434 1248 1119 781 280 279 78 ScaI AGT'ACT Cuts at: 0 111 3464 4355 6219 Size: 111 3353 891 1864 Fragments arranged by size: 3353 1864 891 111 SexAI A'CCwGG_T Cuts at: 0 1113 2194 4014 6219 Size: 1113 1081 1820 2205 Fragments arranged by size: 2205 1820 1113 1081 SfcI C'TryA_G Cuts at: 0 822 948 1439 2824 2984 4704 4714 4921 Size: 822 126 491 1385 160 1720 10 207 Cuts at: 4921 5115 5193 5203 5235 5458 5846 6219 Size: 194 78 10 32 223 388 373 Fragments arranged by size: 1720 1385 822 491 388 373 223 207 194 160 126 78 32 10 10 SmlI C'TyrA_G Cuts at: 0 352 783 1182 3959 4694 5252 6219 Size: 352 431 399 2777 735 558 967 Fragments arranged by size: 2777 967 735 558 431 399 352 SphI G_CATG'C Cuts at: 0 5202 6219 Size: 5202 1017 SspI AAT'ATT Cuts at: 0 2286 3133 3315 5712 6219 Size: 2286 847 182 2397 507 Fragments arranged by size: 2397 2286 847 507 182 StuI AGG'CCT Cuts at: 0 200 781 3375 4702 5274 6219 Size: 200 581 2594 1327 572 945 Fragments arranged by size: 2594 1327 945 581 572 200 StyI C'CwwG_G Cuts at: 0 117 275 831 1390 3839 4466 4601 6219 Size: 117 158 556 559 2449 627 135 1618 Fragments arranged by size: 2449 1618 627 559 556 158 135 117 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 749 1047 1716 3852 5179 5346 5827 6103 Size: 749 298 669 2136 1327 167 481 276 Cuts at: 6103 6219 Size: 116 Fragments arranged by size: 2136 1327 749 669 481 298 276 167 116 TatI w'GTAC_w Cuts at: 0 109 512 3114 3381 3462 3818 4353 6140 Size: 109 403 2602 267 81 356 535 1787 Cuts at: 6140 6219 Size: 79 Fragments arranged by size: 2602 1787 535 403 356 267 109 81 79 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1530 1562 1789 1821 5585 5617 6219 Size: 1530 32 227 32 3764 32 602 Fragments arranged by size: 3764 1530 602 227 32 32 32 XbaI T'CTAG_A Cuts at: 0 283 2641 6219 Size: 283 2358 3578 Fragments arranged by size: 3578 2358 283 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 929 2430 3586 4816 4944 6025 6219 Size: 929 1501 1156 1230 128 1081 194 Fragments arranged by size: 1501 1230 1156 1081 929 194 128 XmnI GAAnn'nnTTC Cuts at: 0 1093 3314 6219 Size: 1093 2221 2905 Fragments arranged by size: 2905 2221 1093 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflII AflIII AlfI AloI AlwNI ApoI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BlpI Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI DraI DraIII DrdI EaeI EarI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NsiI NspI PasI PciI PflMI PfoI PpiI PpuMI PshAI PsrI PstI PvuII RsrII SalI SanDI SapI ScaI SexAI SfcI SmlI SphI SspI StuI StyI TaqII TatI TstI XbaI XcmI XmnI Enzymes that do not cut: AatII AfeI AgeI AhdI AleI ApaI ApaLI AscI AseI AsiSI AvrII BbeI BbvCI BglI BglII BmgBI BplI BsaAI BsaBI BspEI BssHII BstBI BstZ17I BtgZI ClaI EagI EciI EcoICRI EcoNI EcoRV FseI FspI FspAI KasI MluI NarI NotI NruI PacI PmeI PmlI PsiI PspOMI PspXI PvuI SacI SacII SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI Tth111I XhoI XmaI ZraI