Restriction map for
pFN21AE2767
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# NO CUT ENZYMES ( 61 enzymes) #
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AatII Acc65I AclI AcsI AgeI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AvrII BciVI BlnI
BsaBI BspDI BspLU11I Bst1107I BstBI
BstZ17I ClaI CpoI DraI DrdI
Eco47III EcoRI EcoT22I FseI HpaI
KpnI MamI MfeI MluI MunI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RsrII SalI SbfI
SfuI SnaBI SpeI SphI Sse8387I
SspI SwaI Tsp509I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 21 enzymes) #
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AccI 2560 GTCTAC GT^[AC][GT]AC
AccIII 647 TCCGGA T^CCGGA
AflII 1981 CTTAAG C^TTAAG
AviII 2336 TGCGCA TGC^GCA
BamHI 1094 GGATCC G^GATCC
BclI 50 TGATCA T^GATCA
BfrI 1981 CTTAAG C^TTAAG
BglII 2570 AGATCT A^GATCT
BseAI 647 TCCGGA T^CCGGA
BsiWI 2829 CGTACG C^GTACG
BspEI 647 TCCGGA T^CCGGA
BspHI 839 TCATGA T^CATGA
BssHII 3898 GCGCGC G^CGCGC
EcoRV 5008 GATATC GAT^ATC
FbaI 50 TGATCA T^GATCA
FspI 2336 TGCGCA TGC^GCA
MroI 647 TCCGGA T^CCGGA
NotI 4662 GCGGCCGC GC^GGCCGC
PshAI 1146 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 839 TCATGA T^CATGA
SgrAI 1917 CACCGGCG C[GA]^CCGG[CT]G
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# TWO CUT ENZYMES ( 21 enzymes) #
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AhdI 907 GACTACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2968 GACGTGGTGTC
AocI 694 CCTGAGG CC^T[ACGT]AGG
2854 CCTGAGG
AspEI 907 GACTACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2968 GACGTGGTGTC
BbvCI 1728 CCTCAGC CCTCAGC(-5/-2)
5115 CCTCAGC
BcgI 1660 CGAGGACGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4508 CGACCACACTGC
BfaI 1123 CTAG C^TAG
5323 CTAG
BsmI 1948 GAATGC GAATGC(1/-1)
5065 GCATTC
BssSI 735 CACGAG CACGAG(-5/-1)
3336 CACGAG
Bsu36I 694 CCTGAGG CC^T[ACGT]AGG
2854 CCTGAGG
Eam1105I 907 GACTACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2968 GACGTGGTGTC
EarI 5251 CTCTTC CTCTTC(1/4)
105 GAAGAG
Eco81I 694 CCTGAGG CC^T[ACGT]AGG
2854 CCTGAGG
HindIII 1003 AAGCTT A^AGCTT
2698 AAGCTT
Ksp632I 5251 CTCTTC CTCTTC(1/4)
105 GAAGAG
MaeI 1123 CTAG C^TAG
5323 CTAG
MseI 1982 TTAA T^TAA
2270 TTAA
SacI 3544 GAGCTC GAGCT^C
4183 GAGCTC
SapI 105 GAAGAGC GCTCTTC(1/4)
5250 GCTCTTC
ScaI 2201 AGTACT AGT^ACT
3479 AGTACT
SfiI 2679 GGCCAAGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4988 GGCCACGGAGGCC
Tru9I 1982 TTAA T^TAA
2270 TTAA
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# 3 OR MORE CUT ENZYMES ( 155 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaI
ApaLI AspHI AspI AvaI AvaII
BalI BanI BanII BbeI BbrPI
BbsI BbvI BcnI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
Bsp1407I BspMI BsrBI BsrDI BsrFI
BsrGI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EagI EclXI Eco52I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI KspI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflFI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SacII Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SfoI SmaI SmlI
SspBI StuI StyI TaqI TfiI
TseI Tsp45I TspRI Tth111I TthHB8I
Van91I XcmI XhoII XmaCI XmaI