(Linear) (Six-base) MAPSORT of: KIEE2767.seq Check: 3447 from: 1 to: 5466 KIEE2767.orf With 182 enzymes: SgfI * July 3, 2020 20:21 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 779 1375 1750 2703 3162 3524 5466 Size: 779 596 375 953 459 362 1942 Fragments arranged by size: 1942 953 779 596 459 375 362 AccI GT'mk_AC Cuts at: 0 2561 5466 Size: 2561 2905 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 589 2518 2716 5466 Size: 589 1929 198 2750 Fragments arranged by size: 2750 1929 589 198 AflII C'TTAA_G Cuts at: 0 1981 5466 Size: 1981 3485 AflIII A'CryG_T Cuts at: 0 1265 1939 4425 4787 5466 Size: 1265 674 2486 362 679 Fragments arranged by size: 2486 1265 679 674 362 AhdI GACnn_n'nnGTC Cuts at: 0 912 2973 5466 Size: 912 2061 2493 Fragments arranged by size: 2493 2061 912 AleI CACnn'nnGTG Cuts at: 0 928 2647 5466 Size: 928 1719 2819 Fragments arranged by size: 2819 1719 928 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 102 787 2636 5466 Size: 102 685 1849 2830 Fragments arranged by size: 2830 1849 685 102 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 5437 5466 Size: 5437 29 AlwNI CAG_nnn'CTG Cuts at: 0 465 2880 2911 4698 5355 5466 Size: 465 2415 31 1787 657 111 Fragments arranged by size: 2415 1787 657 465 111 31 ApaI G_GGCC'C Cuts at: 0 1122 1242 2081 3270 3806 4247 4310 4622 Size: 1122 120 839 1189 536 441 63 312 Cuts at: 4622 4806 5349 5466 Size: 184 543 117 Fragments arranged by size: 1189 1122 839 543 536 441 312 184 120 117 63 ApaLI G'TGCA_C Cuts at: 0 732 1362 2098 4864 5466 Size: 732 630 736 2766 602 Fragments arranged by size: 2766 736 732 630 602 AvaI C'yCGr_G Cuts at: 0 470 1133 1866 2510 2786 4303 4353 5089 Size: 470 663 733 644 276 1517 50 736 Cuts at: 5089 5466 Size: 377 Fragments arranged by size: 1517 736 733 663 644 470 377 276 50 BamHI G'GATC_C Cuts at: 0 1094 5466 Size: 1094 4372 BanI G'GyrC_C Cuts at: 0 752 817 860 880 918 1915 2068 2445 Size: 752 65 43 20 38 997 153 377 Cuts at: 2445 2667 2835 3091 3382 3531 4233 4408 5466 Size: 222 168 256 291 149 702 175 1058 Fragments arranged by size: 1058 997 752 702 377 291 256 222 175 168 153 149 65 43 38 20 BanII G_rGCy'C Cuts at: 0 385 578 1122 1242 2081 2128 3270 3548 Size: 385 193 544 120 839 47 1142 278 Cuts at: 3548 3603 3806 4130 4187 4247 4310 4423 4622 Size: 55 203 324 57 60 63 113 199 Cuts at: 4622 4806 4879 4960 5252 5318 5349 5466 Size: 184 73 81 292 66 31 117 Fragments arranged by size: 1142 839 544 385 324 292 278 203 199 193 184 120 117 113 81 73 66 63 60 57 55 47 31 BbeI G_GCGC'C Cuts at: 0 756 821 2072 2671 3386 4237 5466 Size: 756 65 1251 599 715 851 1229 Fragments arranged by size: 1251 1229 851 756 715 599 65 BbsI GAAGACnn'nnnn_ Cuts at: 0 771 1180 2282 4769 5466 Size: 771 409 1102 2487 697 Fragments arranged by size: 2487 1102 771 697 409 BbvCI CC'TCA_GC Cuts at: 0 1729 5116 5466 Size: 1729 3387 350 Fragments arranged by size: 3387 1729 350 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1649 1683 4497 4531 5466 Size: 1649 34 2814 34 935 Fragments arranged by size: 2814 1649 935 34 34 BclI T'GATC_A Cuts at: 0 50 5466 Size: 50 5416 BglI GCCn_nnn'nGGC Cuts at: 0 1136 2123 2227 2454 2686 3598 3963 4242 Size: 1136 987 104 227 232 912 365 279 Cuts at: 4242 4817 4995 5466 Size: 575 178 471 Fragments arranged by size: 1136 987 912 575 471 365 279 232 227 178 104 BglII A'GATC_T Cuts at: 0 2570 5466 Size: 2570 2896 BlpI GC'TnA_GC Cuts at: 0 3247 3712 3722 4444 4975 5466 Size: 3247 465 10 722 531 491 Fragments arranged by size: 3247 722 531 491 465 10 Bme1580I G_kGCm'C Cuts at: 0 736 883 923 1122 1242 1366 1565 1918 Size: 736 147 40 199 120 124 199 353 Cuts at: 1918 2081 2102 2450 2840 2903 3270 3536 3659 Size: 163 21 348 390 63 367 266 123 Cuts at: 3659 3806 4247 4310 4433 4583 4622 4806 4868 Size: 147 441 63 123 150 39 184 62 Cuts at: 4868 4929 5132 5349 5466 Size: 61 203 217 117 Fragments arranged by size: 736 441 390 367 353 348 266 217 203 199 199 184 163 150 147 147 124 123 123 120 117 63 63 62 61 40 39 21 BmgBI CAC'GTC Cuts at: 0 1777 2364 2898 2970 3240 5466 Size: 1777 587 534 72 270 2226 Fragments arranged by size: 2226 1777 587 534 270 72 BmrI ACTGGGnnnn_n' Cuts at: 0 94 885 1042 1193 4916 5466 Size: 94 791 157 151 3723 550 Fragments arranged by size: 3723 791 550 157 151 94 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 690 1173 5466 Size: 690 483 4293 Fragments arranged by size: 4293 690 483 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 379 397 1096 1473 2575 3205 5377 5466 Size: 379 18 699 377 1102 630 2172 89 Fragments arranged by size: 2172 1102 699 630 379 377 89 18 Bpu10I CC'TnA_GC Cuts at: 0 478 1729 3404 3602 3923 4417 4954 5116 Size: 478 1251 1675 198 321 494 537 162 Cuts at: 5116 5431 5466 Size: 315 35 Fragments arranged by size: 1675 1251 537 494 478 321 315 198 162 35 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 430 1264 1315 2335 2725 5466 Size: 430 834 51 1020 390 2741 Fragments arranged by size: 2741 1020 834 430 390 51 BsaI GGTCTCn'nnnn_ Cuts at: 0 259 3075 3727 5466 Size: 259 2816 652 1739 Fragments arranged by size: 2816 1739 652 259 BsaAI yAC'GTr Cuts at: 0 1251 1266 1940 4166 4428 4788 5466 Size: 1251 15 674 2226 262 360 678 Fragments arranged by size: 2226 1251 678 674 360 262 15 BsaHI Gr'CG_yC Cuts at: 0 753 818 2069 2668 2783 3383 3672 4007 Size: 753 65 1251 599 115 600 289 335 Cuts at: 4007 4226 4234 5466 Size: 219 8 1232 Fragments arranged by size: 1251 1232 753 600 599 335 289 219 115 65 8 BsaWI w'CCGG_w Cuts at: 0 647 2575 3326 3839 5466 Size: 647 1928 751 513 1627 Fragments arranged by size: 1928 1627 751 647 513 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1039 1069 1406 1436 1540 1570 1873 1903 Size: 1039 30 337 30 104 30 303 30 Cuts at: 1903 2573 2603 4639 4669 5466 Size: 670 30 2036 30 797 Fragments arranged by size: 2036 1039 797 670 337 303 104 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 277 355 1017 1093 2008 2143 2146 2149 Size: 277 78 662 76 915 135 3 3 Cuts at: 2149 2557 3684 4156 4209 4539 5063 5466 Size: 408 1127 472 53 330 524 403 Fragments arranged by size: 1127 915 662 524 472 408 403 330 277 135 78 76 53 3 3 BseYI C'CCAG_C Cuts at: 0 16 2522 3412 3658 3707 3716 3872 3914 Size: 16 2506 890 246 49 9 156 42 Cuts at: 3914 3953 4087 4134 4820 4979 5232 5348 5466 Size: 39 134 47 686 159 253 116 118 Fragments arranged by size: 2506 890 686 253 246 159 156 134 118 116 49 47 42 39 16 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 96 135 1249 1461 1918 2022 4357 5167 Size: 96 39 1114 212 457 104 2335 810 Cuts at: 5167 5466 Size: 299 Fragments arranged by size: 2335 1114 810 457 299 212 104 96 39 BsiEI CG_ry'CG Cuts at: 0 1542 2925 4666 5466 Size: 1542 1383 1741 800 Fragments arranged by size: 1741 1542 1383 800 BsiHKAI G_wGCw'C Cuts at: 0 736 1250 1366 1537 2102 3548 4187 4322 Size: 736 514 116 171 565 1446 639 135 Cuts at: 4322 4868 4918 5466 Size: 546 50 548 Fragments arranged by size: 1446 736 639 565 548 546 514 171 135 116 50 BsiWI C'GTAC_G Cuts at: 0 2829 5466 Size: 2829 2637 BsmI GAATG_Cn' Cuts at: 0 1954 5065 5466 Size: 1954 3111 401 Fragments arranged by size: 3111 1954 401 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2960 3696 4098 4551 5466 Size: 2960 736 402 453 915 Fragments arranged by size: 2960 915 736 453 402 Bsp1286I G_dGCh'C Cuts at: 0 385 578 736 883 923 1122 1242 1250 Size: 385 193 158 147 40 199 120 8 Cuts at: 1250 1366 1537 1565 1918 2081 2102 2128 2450 Size: 116 171 28 353 163 21 26 322 Cuts at: 2450 2840 2903 3270 3536 3548 3603 3659 3806 Size: 390 63 367 266 12 55 56 147 Cuts at: 3806 4130 4187 4247 4310 4322 4423 4433 4583 Size: 324 57 60 63 12 101 10 150 Cuts at: 4583 4622 4806 4868 4879 4918 4929 4960 5132 Size: 39 184 62 11 39 11 31 172 Cuts at: 5132 5252 5318 5349 5466 Size: 120 66 31 117 Fragments arranged by size: 390 385 367 353 324 322 266 199 193 184 172 171 163 158 150 147 147 120 120 117 116 101 66 63 63 62 60 57 56 55 40 39 39 31 31 28 26 21 12 12 11 11 10 8 BspEI T'CCGG_A Cuts at: 0 647 5466 Size: 647 4819 BspHI T'CATG_A Cuts at: 0 839 5466 Size: 839 4627 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 779 1375 1474 1750 2501 2703 3162 3524 Size: 779 596 99 276 751 202 459 362 Cuts at: 3524 4160 5466 Size: 636 1306 Fragments arranged by size: 1306 779 751 636 596 459 362 276 202 99 BsrBI CCG'CTC Cuts at: 0 345 669 1100 2169 2675 3927 4016 4490 Size: 345 324 431 1069 506 1252 89 474 Cuts at: 4490 4662 4986 5466 Size: 172 324 480 Fragments arranged by size: 1252 1069 506 480 474 431 345 324 324 172 89 BsrDI GCAATG_nn' Cuts at: 0 1659 1740 1761 5466 Size: 1659 81 21 3705 Fragments arranged by size: 3705 1659 81 21 BsrFI r'CCGG_y Cuts at: 0 173 1480 1918 2685 3726 3910 5096 5466 Size: 173 1307 438 767 1041 184 1186 370 Fragments arranged by size: 1307 1186 1041 767 438 370 184 173 BsrGI T'GTAC_A Cuts at: 0 395 2580 4043 5466 Size: 395 2185 1463 1423 Fragments arranged by size: 2185 1463 1423 395 BssHII G'CGCG_C Cuts at: 0 3898 5466 Size: 3898 1568 BssSI C'ACGA_G Cuts at: 0 735 3336 5466 Size: 735 2601 2130 Fragments arranged by size: 2601 2130 735 BstAPI GCAn_nnn'nTGC Cuts at: 0 2222 2911 5265 5466 Size: 2222 689 2354 201 Fragments arranged by size: 2354 2222 689 201 BstEII G'GTnAC_C Cuts at: 0 1152 2379 3702 5025 5466 Size: 1152 1227 1323 1323 441 Fragments arranged by size: 1323 1323 1227 1152 441 BstXI CCAn_nnnn'nTGG Cuts at: 0 828 1065 2442 2970 4599 5466 Size: 828 237 1377 528 1629 867 Fragments arranged by size: 1629 1377 867 828 528 237 BstYI r'GATC_y Cuts at: 0 671 1094 2570 3425 5466 Size: 671 423 1476 855 2041 Fragments arranged by size: 2041 1476 855 671 423 Bsu36I CC'TnA_GG Cuts at: 0 695 2855 5466 Size: 695 2160 2611 Fragments arranged by size: 2611 2160 695 BtgI C'CryG_G Cuts at: 0 22 206 386 875 1542 1673 2054 3006 Size: 22 184 180 489 667 131 381 952 Cuts at: 3006 3122 3698 4239 4651 4990 5466 Size: 116 576 541 412 339 476 Fragments arranged by size: 952 667 576 541 489 476 412 381 339 184 180 131 116 22 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 4064 5466 Size: 4064 1402 BtsI GCAGTG_nn' Cuts at: 0 1803 1906 2768 2909 4299 4513 4946 5466 Size: 1803 103 862 141 1390 214 433 520 Fragments arranged by size: 1803 1390 862 520 433 214 141 103 DraIII CAC_nnn'GTG Cuts at: 0 119 1569 2262 4326 4440 5466 Size: 119 1450 693 2064 114 1026 Fragments arranged by size: 2064 1450 1026 693 119 114 EaeI y'GGCC_r Cuts at: 0 203 1458 1539 1779 1794 2678 2922 3947 Size: 203 1255 81 240 15 884 244 1025 Cuts at: 3947 4202 4332 4663 4987 5466 Size: 255 130 331 324 479 Fragments arranged by size: 1255 1025 884 479 331 324 255 244 240 203 130 81 15 EagI C'GGCC_G Cuts at: 0 1539 2922 4663 5466 Size: 1539 1383 1741 803 Fragments arranged by size: 1741 1539 1383 803 EarI CTCTTCn'nnn_ Cuts at: 0 100 5257 5466 Size: 100 5157 209 Fragments arranged by size: 5157 209 100 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 208 3217 4890 5466 Size: 208 3009 1673 576 Fragments arranged by size: 3009 1673 576 208 EcoICRI GAG'CTC Cuts at: 0 3546 4185 5466 Size: 3546 639 1281 Fragments arranged by size: 3546 1281 639 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 379 397 589 1096 1473 2518 2575 2716 Size: 379 18 192 507 377 1045 57 141 Cuts at: 2716 3205 5377 5466 Size: 489 2172 89 Fragments arranged by size: 2172 1045 507 489 379 377 192 141 89 57 18 EcoNI CCTnn'n_nnAGG Cuts at: 0 1513 1876 2554 5466 Size: 1513 363 678 2912 Fragments arranged by size: 2912 1513 678 363 EcoO109I rG'GnC_Cy Cuts at: 0 71 96 474 1119 1187 1239 1881 2726 Size: 71 25 378 645 68 52 642 845 Cuts at: 2726 3266 3309 3613 3919 4244 4307 4618 5218 Size: 540 43 304 306 325 63 311 600 Cuts at: 5218 5345 5466 Size: 127 121 Fragments arranged by size: 845 645 642 600 540 378 325 311 306 304 127 121 71 68 63 52 43 25 EcoRV GAT'ATC Cuts at: 0 5010 5466 Size: 5010 456 FspI TGC'GCA Cuts at: 0 2338 5466 Size: 2338 3128 HaeII r_GCGC'y Cuts at: 0 180 756 821 2072 2671 3386 4218 4237 Size: 180 576 65 1251 599 715 832 19 Cuts at: 4237 5309 5466 Size: 1072 157 Fragments arranged by size: 1251 1072 832 715 599 576 180 157 65 19 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 656 688 898 930 1039 1071 1375 1407 Size: 656 32 210 32 109 32 304 32 Cuts at: 1407 1873 1905 2959 2991 3487 3519 4462 4494 Size: 466 32 1054 32 496 32 943 32 Cuts at: 4494 5466 Size: 972 Fragments arranged by size: 1054 972 943 656 496 466 304 210 109 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 3201 3780 3825 5466 Size: 3201 579 45 1641 Fragments arranged by size: 3201 1641 579 45 HindIII A'AGCT_T Cuts at: 0 1003 2698 5466 Size: 1003 1695 2768 Fragments arranged by size: 2768 1695 1003 KasI G'GCGC_C Cuts at: 0 752 817 2068 2667 3382 4233 5466 Size: 752 65 1251 599 715 851 1233 Fragments arranged by size: 1251 1233 851 752 715 599 65 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 440 1082 1127 1346 1681 4247 5466 Size: 440 642 45 219 335 2566 1219 Fragments arranged by size: 2566 1219 642 440 335 219 45 MscI TGG'CCA Cuts at: 0 1460 1781 3949 5466 Size: 1460 321 2168 1517 Fragments arranged by size: 2168 1517 1460 321 MslI CAynn'nnrTG Cuts at: 0 403 928 1253 2329 2647 2896 3121 5440 Size: 403 525 325 1076 318 249 225 2319 Cuts at: 5440 5466 Size: 26 Fragments arranged by size: 2319 1076 525 403 325 318 249 225 26 MspA1I CmG'CkG Cuts at: 0 86 208 219 727 1233 1275 1799 2342 Size: 86 122 11 508 506 42 524 543 Cuts at: 2342 2372 2397 2526 2877 3008 3412 3711 4211 Size: 30 25 129 351 131 404 299 500 Cuts at: 4211 4653 4668 4695 4938 4974 5232 5265 5423 Size: 442 15 27 243 36 258 33 158 Cuts at: 5423 5466 Size: 43 Fragments arranged by size: 543 524 508 506 500 442 404 351 299 258 243 158 131 129 122 86 43 42 36 33 30 27 25 15 11 NaeI GCC'GGC Cuts at: 0 2687 3728 3912 5098 5466 Size: 2687 1041 184 1186 368 Fragments arranged by size: 2687 1186 1041 368 184 NarI GG'CG_CC Cuts at: 0 753 818 2069 2668 3383 4234 5466 Size: 753 65 1251 599 715 851 1232 Fragments arranged by size: 1251 1232 851 753 715 599 65 NcoI C'CATG_G Cuts at: 0 22 1673 2054 5466 Size: 22 1651 381 3412 Fragments arranged by size: 3412 1651 381 22 NgoMIV G'CCGG_C Cuts at: 0 2685 3726 3910 5096 5466 Size: 2685 1041 184 1186 370 Fragments arranged by size: 2685 1186 1041 370 184 NotI GC'GGCC_GC Cuts at: 0 4663 5466 Size: 4663 803 NspI r_CATG'y Cuts at: 0 2289 2394 5386 5466 Size: 2289 105 2992 80 Fragments arranged by size: 2992 2289 105 80 PasI CC'CwG_GG Cuts at: 0 2074 4520 4614 5340 5466 Size: 2074 2446 94 726 126 Fragments arranged by size: 2446 2074 726 126 94 PflMI CCAn_nnn'nTGG Cuts at: 0 23 774 2538 5355 5466 Size: 23 751 1764 2817 111 Fragments arranged by size: 2817 1764 751 111 23 PfoI T'CCnGG_A Cuts at: 0 2018 2804 3047 3182 3418 5466 Size: 2018 786 243 135 236 2048 Fragments arranged by size: 2048 2018 786 243 236 135 PmlI CAC'GTG Cuts at: 0 1251 1266 1940 4166 4428 5466 Size: 1251 15 674 2226 262 1038 Fragments arranged by size: 2226 1251 1038 674 262 15 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1585 1617 3124 3156 5466 Size: 1585 32 1507 32 2310 Fragments arranged by size: 2310 1585 1507 32 32 PpuMI rG'GwC_Cy Cuts at: 0 71 474 1187 1881 3309 3613 5218 5466 Size: 71 403 713 694 1428 304 1605 248 Fragments arranged by size: 1605 1428 713 694 403 304 248 71 PshAI GACnn'nnGTC Cuts at: 0 1150 5466 Size: 1150 4316 PspOMI G'GGCC_C Cuts at: 0 1118 1238 2077 3266 3802 4243 4306 4618 Size: 1118 120 839 1189 536 441 63 312 Cuts at: 4618 4802 5345 5466 Size: 184 543 121 Fragments arranged by size: 1189 1118 839 543 536 441 312 184 121 120 63 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 2545 2577 5116 5148 5466 Size: 2545 32 2539 32 318 Fragments arranged by size: 2545 2539 318 32 32 PstI C_TGCA'G Cuts at: 0 662 722 1046 1405 2775 3173 4673 4973 Size: 662 60 324 359 1370 398 1500 300 Cuts at: 4973 5466 Size: 493 Fragments arranged by size: 1500 1370 662 493 398 359 324 300 60 PvuII CAG'CTG Cuts at: 0 727 1233 1275 2526 2877 3711 4695 4974 Size: 727 506 42 1251 351 834 984 279 Cuts at: 4974 5232 5466 Size: 258 234 Fragments arranged by size: 1251 984 834 727 506 351 279 258 234 42 SacI G_AGCT'C Cuts at: 0 3548 4187 5466 Size: 3548 639 1279 Fragments arranged by size: 3548 1279 639 SacII CC_GC'GG Cuts at: 0 209 3009 4654 5466 Size: 209 2800 1645 812 Fragments arranged by size: 2800 1645 812 209 SanDI GG'GwC_CC Cuts at: 0 3309 3613 5466 Size: 3309 304 1853 Fragments arranged by size: 3309 1853 304 SapI GCTCTTCn'nnn_ Cuts at: 0 100 5257 5466 Size: 100 5157 209 Fragments arranged by size: 5157 209 100 ScaI AGT'ACT Cuts at: 0 2203 3481 5466 Size: 2203 1278 1985 Fragments arranged by size: 2203 1985 1278 SexAI A'CCwGG_T Cuts at: 0 1223 3014 4322 5021 5466 Size: 1223 1791 1308 699 445 Fragments arranged by size: 1791 1308 1223 699 445 SfcI C'TryA_G Cuts at: 0 658 718 987 1042 1401 2043 2562 2771 Size: 658 60 269 55 359 642 519 209 Cuts at: 2771 3169 3618 4669 4677 4969 5466 Size: 398 449 1051 8 292 497 Fragments arranged by size: 1051 658 642 519 497 449 398 359 292 269 209 60 55 8 SfiI GGCCn_nnn'nGGCC Cuts at: 0 2686 4995 5466 Size: 2686 2309 471 Fragments arranged by size: 2686 2309 471 SfoI GGC'GCC Cuts at: 0 754 819 2070 2669 3384 4235 5466 Size: 754 65 1251 599 715 851 1231 Fragments arranged by size: 1251 1231 851 754 715 599 65 SgrAI Cr'CCGG_yG Cuts at: 0 1918 5466 Size: 1918 3548 SmaI CCC'GGG Cuts at: 0 1868 2788 4305 4355 5466 Size: 1868 920 1517 50 1111 Fragments arranged by size: 1868 1517 1111 920 50 SmlI C'TyrA_G Cuts at: 0 445 1243 1330 1981 2350 2740 5466 Size: 445 798 87 651 369 390 2726 Fragments arranged by size: 2726 798 651 445 390 369 87 StuI AGG'CCT Cuts at: 0 314 558 1084 1139 2221 3157 5466 Size: 314 244 526 55 1082 936 2309 Fragments arranged by size: 2309 1082 936 526 314 244 55 StyI C'CwwG_G Cuts at: 0 22 74 309 1673 2054 5043 5466 Size: 22 52 235 1364 381 2989 423 Fragments arranged by size: 2989 1364 423 381 235 52 22 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 202 781 904 1673 2988 3658 3967 4426 Size: 202 579 123 769 1315 670 309 459 Cuts at: 4426 5311 5452 5466 Size: 885 141 14 Fragments arranged by size: 1315 885 769 670 579 459 309 202 141 123 14 TatI w'GTAC_w Cuts at: 0 395 893 2201 2580 3479 4043 5276 5466 Size: 395 498 1308 379 899 564 1233 190 Fragments arranged by size: 1308 1233 899 564 498 395 379 190 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1406 1421 1438 1453 1516 1540 1548 1572 Size: 1406 15 17 15 63 24 8 24 Cuts at: 1572 2190 2222 3477 3509 4639 4671 5466 Size: 618 32 1255 32 1130 32 795 Fragments arranged by size: 1406 1255 1130 795 618 63 32 32 32 24 24 17 15 15 8 Tth111I GACn'n_nGTC Cuts at: 0 529 1185 2365 5466 Size: 529 656 1180 3101 Fragments arranged by size: 3101 1180 656 529 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 36 1789 3942 5466 Size: 36 1753 2153 1524 Fragments arranged by size: 2153 1753 1524 36 XmaI C'CCGG_G Cuts at: 0 1866 2786 4303 4353 5466 Size: 1866 920 1517 50 1113 Fragments arranged by size: 1866 1517 1113 920 50 Enzymes that do cut: AarI AccI AcuI AflII AflIII AhdI AleI AlfI AloI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FspI HaeII Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PsrI PstI PvuII SacI SacII SanDI SapI ScaI SexAI SfcI SfiI SfoI SgrAI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AatII Acc65I AclI AfeI AgeI ApoI AscI AseI AsiSI AvrII BaeI BciVI BfrBI BmtI BsaBI BstBI BstZ17I ClaI CspCI DraI DrdI EcoRI FalI FseI FspAI HpaI KpnI MfeI MluI NdeI NheI NruI NsiI PacI PciI PmeI PsiI PspXI PvuI RsrII SalI SbfI SgfI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI XmnI ZraI