(Circular) (Six-base) MAPSORT of: pFN21AE2600.seq Check: 5252 from: 1 to: 5596 pFN21AE2600 5596 bp With 182 enzymes: SgfI * January 12, 2016 11:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3406 278 Size: 53 83 186 936 1870 2468 Fragments arranged by size: 2468 1870 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2939 1937 Size: 1002 4594 Acc65I G'GTAC_C Cuts at: 2917 2917 Size: 5596 AclI AA'CG_TT Cuts at: 1563 3723 4096 1563 Size: 2160 373 3063 Fragments arranged by size: 3063 2160 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2714 3659 5043 5311 1632 Size: 1082 945 1384 268 1917 Fragments arranged by size: 1917 1384 1082 945 268 AfeI AGC'GCT Cuts at: 2264 2576 2264 Size: 312 5284 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5399 AflIII A'CryG_T Cuts at: 1203 4495 5472 1203 Size: 3292 977 1327 Fragments arranged by size: 3292 1327 977 AgeI A'CCGG_T Cuts at: 4427 4427 Size: 5596 AhdI GACnn_n'nnGTC Cuts at: 2593 4325 2593 Size: 1732 3864 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5596 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5596 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2609 2641 2609 Size: 32 5564 AlwNI CAG_nnn'CTG Cuts at: 2323 3339 4911 2323 Size: 1016 1572 3008 Fragments arranged by size: 3008 1572 1016 ApaLI G'TGCA_C Cuts at: 2142 2870 3653 4809 2142 Size: 728 783 1156 2929 Fragments arranged by size: 2929 1156 783 728 ApoI r'AATT_y Cuts at: 1493 2905 3131 3283 5392 1493 Size: 1412 226 152 2109 1697 Fragments arranged by size: 2109 1697 1412 226 152 AseI AT'TA_AT Cuts at: 160 1032 4150 160 Size: 872 3118 1606 Fragments arranged by size: 3118 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5596 AvaI C'yCGr_G Cuts at: 1870 1943 2095 2921 1870 Size: 73 152 826 4545 Fragments arranged by size: 4545 826 152 73 BamHI G'GATC_C Cuts at: 2926 3293 2926 Size: 367 5229 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2917 4372 618 Size: 325 217 627 1130 1455 1842 Fragments arranged by size: 1842 1455 1130 627 325 217 BanII G_rGCy'C Cuts at: 1391 2096 2343 2477 2915 1391 Size: 705 247 134 438 4072 Fragments arranged by size: 4072 705 438 247 134 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2454 2682 928 Size: 971 555 228 3842 Fragments arranged by size: 3842 971 555 228 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2123 2157 3787 3821 1903 Size: 34 50 34 102 34 1630 34 3678 Fragments arranged by size: 3678 1630 102 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3488 4698 3488 Size: 1210 4386 BclI T'GATC_A Cuts at: 1548 1548 Size: 5596 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4207 136 Size: 107 122 71 631 605 2535 1525 Fragments arranged by size: 2535 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5591 5591 Size: 5596 BlpI GC'TnA_GC Cuts at: 3013 3013 Size: 5596 Bme1580I G_kGCm'C Cuts at: 1790 2146 2874 3657 4813 1790 Size: 356 728 783 1156 2573 Fragments arranged by size: 2573 1156 783 728 356 BmgBI CAC'GTC Cuts at: 1318 2220 2869 1318 Size: 902 649 4045 Fragments arranged by size: 4045 902 649 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2146 3327 4285 Size: 416 53 83 399 24 729 1181 958 Cuts at: 4285 442 Size: 1753 Fragments arranged by size: 1753 1181 958 729 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5596 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2388 2470 4256 1239 Size: 1149 82 1786 2579 Fragments arranged by size: 2579 1786 1149 82 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3721 4586 4884 3721 Size: 865 298 4433 Fragments arranged by size: 4433 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4259 882 Size: 617 2760 2219 Fragments arranged by size: 2760 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3387 493 Size: 711 2183 2702 Fragments arranged by size: 2702 2183 711 BsaBI GATnn'nnATC Cuts at: 2540 5590 2540 Size: 3050 2546 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3403 3785 4420 275 Size: 53 83 186 936 1870 382 635 1451 Fragments arranged by size: 1870 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4029 4427 4701 4848 5259 4029 Size: 398 274 147 411 4366 Fragments arranged by size: 4366 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2462 2492 2609 2639 1178 Size: 30 165 30 1059 30 117 30 4135 Fragments arranged by size: 4135 1059 165 117 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2614 1186 Size: 1428 4168 BseYI C'CCAG_C Cuts at: 4799 5295 4799 Size: 496 5100 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5023 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2312 2335 3046 3807 4835 1540 Size: 275 186 311 23 711 761 1028 2301 Fragments arranged by size: 2301 1028 761 711 311 275 186 23 BsiHKAI G_wGCw'C Cuts at: 2096 2146 2606 2874 3657 3742 4813 2096 Size: 50 460 268 783 85 1071 2879 Fragments arranged by size: 2879 1071 783 460 268 85 50 BsmI GAATG_Cn' Cuts at: 3107 3200 3107 Size: 93 5503 BsmBI CGTCTCn'nnnn_ Cuts at: 2488 2488 Size: 5596 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2096 2146 2343 2477 2606 2874 2915 Size: 399 306 50 197 134 129 268 41 Cuts at: 2915 3657 3742 4813 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 306 268 197 134 129 85 50 41 BspEI T'CCGG_A Cuts at: 5259 5259 Size: 5596 BspHI T'CATG_A Cuts at: 3485 5215 3485 Size: 1730 3866 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2391 2951 844 Size: 1547 560 3489 Fragments arranged by size: 3489 1547 560 BsrBI CCG'CTC Cuts at: 1394 1823 3483 1394 Size: 429 1660 3507 Fragments arranged by size: 3507 1660 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4091 4265 65 Size: 1183 2843 174 1396 Fragments arranged by size: 2843 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2071 2459 4240 4427 1700 Size: 110 261 388 1781 187 2869 Fragments arranged by size: 2869 1781 388 261 187 110 BsrGI T'GTAC_A Cuts at: 96 2725 96 Size: 2629 2967 BssHII G'CGCG_C Cuts at: 1925 2157 2374 1925 Size: 232 217 5147 Fragments arranged by size: 5147 232 217 BssSI C'ACGA_G Cuts at: 3656 4668 3656 Size: 1012 4584 BstAPI GCAn_nnn'nTGC Cuts at: 794 2323 794 Size: 1529 4067 BstBI TT'CG_AA Cuts at: 4400 4400 Size: 5596 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5596 BstYI r'GATC_y Cuts at: 1972 2926 3293 3678 3695 5147 5233 5245 5591 Size: 954 367 385 17 1452 86 12 346 Cuts at: 5591 1972 Size: 1977 Fragments arranged by size: 1977 1452 954 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2062 2112 2335 513 Size: 552 997 50 223 3774 Fragments arranged by size: 3774 997 552 223 50 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4857 Fragments arranged by size: 4857 683 56 BtsI GCAGTG_nn' Cuts at: 2470 2777 2872 3112 3906 3926 2470 Size: 307 95 240 794 20 4140 Fragments arranged by size: 4140 794 307 240 95 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5596 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5561 DraI TTT'AAA Cuts at: 2900 2900 Size: 5596 DraIII CAC_nnn'GTG Cuts at: 2500 2500 Size: 5596 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4603 809 Size: 507 3287 1802 Fragments arranged by size: 3287 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2332 3043 3932 Size: 54 1425 50 275 18 502 711 889 Cuts at: 3932 5561 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 711 502 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2332 3043 1537 Size: 275 520 711 4090 Fragments arranged by size: 4090 711 520 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3526 1353 Size: 2173 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2154 4183 4557 4703 387 Size: 1064 138 565 2029 374 146 1280 Fragments arranged by size: 2029 1280 1064 565 374 146 138 EcoICRI GAG'CTC Cuts at: 2094 2094 Size: 5596 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2388 2470 2714 3659 4256 5043 5311 Size: 393 756 82 244 945 597 787 268 Cuts at: 5311 1239 Size: 1524 Fragments arranged by size: 1524 945 787 756 597 393 268 244 82 EcoNI CCTnn'n_nnAGG Cuts at: 2784 2838 2784 Size: 54 5542 EcoO109I rG'GnC_Cy Cuts at: 2108 2108 Size: 5596 EcoRI G'AATT_C Cuts at: 2905 3283 2905 Size: 378 5218 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2818 2818 Size: 5596 FspI TGC'GCA Cuts at: 2224 4102 2224 Size: 1878 3718 FspAI rTGC'GCAy Cuts at: 2224 2224 Size: 5596 HaeII r_GCGC'y Cuts at: 1678 2266 2324 2396 2460 2578 4743 1678 Size: 588 58 72 64 118 2165 2531 Fragments arranged by size: 2531 2165 588 118 72 64 58 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2010 2042 2462 2494 2579 Size: 32 135 32 438 32 420 32 85 Cuts at: 2579 2611 4237 4269 4311 4343 1373 Size: 32 1626 32 42 32 2626 Fragments arranged by size: 2626 1626 438 420 135 85 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2350 2587 2940 3186 669 Size: 988 281 412 237 353 246 3079 Fragments arranged by size: 3079 988 412 353 281 246 237 HindIII A'AGCT_T Cuts at: 748 748 Size: 5596 HpaI GTT'AAC Cuts at: 3186 3186 Size: 5596 KpnI G_GTAC'C Cuts at: 2921 2921 Size: 5596 MfeI C'AATT_G Cuts at: 3195 3195 Size: 5596 MluI A'CGCG_T Cuts at: 5472 5472 Size: 5596 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2421 4710 4894 1305 Size: 1116 2289 184 2007 Fragments arranged by size: 2289 2007 1116 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3774 Fragments arranged by size: 3774 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3554 3913 4072 518 Size: 648 2388 359 159 2042 Fragments arranged by size: 2388 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2011 2036 2064 2314 2337 3012 3689 4837 Size: 405 25 28 250 23 675 677 1148 Cuts at: 4837 5082 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 675 405 250 245 28 25 23 NaeI GCC'GGC Cuts at: 2461 2461 Size: 5596 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5044 NdeI CA'TA_TG Cuts at: 387 387 Size: 5596 NgoMIV G'CCGG_C Cuts at: 2459 2459 Size: 5596 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5596 NotI GC'GGCC_GC Cuts at: 3043 3043 Size: 5596 NspI r_CATG'y Cuts at: 1132 1229 2561 2887 2954 4499 1132 Size: 97 1332 326 67 1545 2229 Fragments arranged by size: 2229 1545 1332 326 97 67 PasI CC'CwG_GG Cuts at: 1348 2718 1348 Size: 1370 4226 PciI A'CATG_T Cuts at: 4495 4495 Size: 5596 PflMI CCAn_nnn'nTGG Cuts at: 1108 2028 1108 Size: 920 4676 PmeI GTTT'AAAC Cuts at: 2900 2900 Size: 5596 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2609 2641 3665 3697 5178 5210 2609 Size: 32 1024 32 1481 32 2995 Fragments arranged by size: 2995 1481 1024 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2108 2108 Size: 5596 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5596 PsiI TTA'TAA Cuts at: 2760 3166 2760 Size: 406 5190 PstI C_TGCA'G Cuts at: 830 2319 2948 830 Size: 1489 629 3478 Fragments arranged by size: 3478 1489 629 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5596 SacI G_AGCT'C Cuts at: 2096 2096 Size: 5596 SacII CC_GC'GG Cuts at: 2065 2338 2065 Size: 273 5323 SalI G'TCGA_C Cuts at: 1936 2938 1936 Size: 1002 4594 SanDI GG'GwC_CC Cuts at: 2108 2108 Size: 5596 SbfI CC_TGCA'GG Cuts at: 2948 2948 Size: 5596 ScaI AGT'ACT Cuts at: 3844 5556 3844 Size: 1712 3884 SfcI C'TryA_G Cuts at: 826 1045 2315 2878 2944 4079 4760 4951 826 Size: 219 1270 563 66 1135 681 191 1471 Fragments arranged by size: 1471 1270 1135 681 563 219 191 66 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5596 SgrAI Cr'CCGG_yG Cuts at: 1700 2071 1700 Size: 371 5225 SmaI CCC'GGG Cuts at: 1872 2923 1872 Size: 1051 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3700 4601 4863 820 Size: 197 926 1757 901 262 1553 Fragments arranged by size: 1757 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3387 493 Size: 2894 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5596 SphI G_CATG'C Cuts at: 1132 2561 2887 2954 1132 Size: 1429 326 67 3774 Fragments arranged by size: 3774 1429 326 67 SspI AAT'ATT Cuts at: 5 52 3520 5 Size: 47 3468 2081 Fragments arranged by size: 3468 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5596 StyI C'CwwG_G Cuts at: 513 1065 2853 3035 513 Size: 552 1788 182 3074 Fragments arranged by size: 3074 1788 552 182 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3623 3640 3793 3978 4423 325 Size: 1255 2043 17 153 185 445 1498 Fragments arranged by size: 2043 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2725 3842 5554 Size: 274 80 33 51 1443 748 1117 1712 Cuts at: 5554 96 Size: 138 Fragments arranged by size: 1712 1443 1117 748 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5556 5588 5556 Size: 32 5564 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5596 XbaI T'CTAG_A Cuts at: 2932 2932 Size: 5596 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5596 XmaI C'CCGG_G Cuts at: 1870 2921 1870 Size: 1051 4545 XmnI GAAnn'nnTTC Cuts at: 3725 3725 Size: 5596 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3404 276 Size: 53 83 186 936 1870 2468 Fragments arranged by size: 2468 1870 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoRV FseI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SapI SexAI SfiI SfoI SrfI SwaI XcmI