################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII Acc65I AccI AclI AflII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BbrPI BbvCI BciVI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsgI BsiEI BsiWI BsmAI BsmBI Bsp1407I BspHI BspLU11I BspMI BsrDI BsrGI BssSI Bst1107I BstAPI BstEII BstPI BstZ17I Bsu36I CelII CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MluI MseI MslI MunI NdeI NheI NotI NruI NsiI NspI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccIII 419 TCCGGA T^CCGGA AcsI 1288 AAATTC [GA]^AATT[CT] AflIII 1134 ACGTGT A^C[GA][CT]GT AlwNI 898 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 1288 AAATTC [GA]^AATT[CT] Asp700I 1149 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 900 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC BcgI 647 CGATCCGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 446 TGATCA T^GATCA BfaI 1123 CTAG C^TAG BglI 152 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BseAI 419 TCCGGA T^CCGGA BseRI 20 CTCCTC GAGGAG(10/8) BsmI 818 GCATTC GAATGC(1/-1) BspDI 970 ATCGAT AT^CGAT BspEI 419 TCCGGA T^CCGGA BsrFI 90 GCCGGC [GA]^CCGG[CT] BstBI 992 TTCGAA TT^CGAA Cfr10I 90 GCCGGC [GA]^CCGG[CT] ClaI 970 ATCGAT AT^CGAT DraII 625 GGGACCC [GA]G^G[ACGT]CC[CT] DrdI 1261 GACCCAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1354 GAAGAG CTCTTC(1/4) EcoO109I 625 GGGACCC [GA]G^G[ACGT]CC[CT] FbaI 446 TGATCA T^GATCA HgaI 409 GACGC GACGC(5/10) Ksp632I 1354 GAAGAG CTCTTC(1/4) MaeI 1123 CTAG C^TAG MroI 419 TCCGGA T^CCGGA NaeI 90 GCCGGC GCC^GGC NgoMIV 90 GCCGGC G^CCGGC NspV 992 TTCGAA TT^CGAA PflFI 900 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 625 GGGACCC [GA]G^G[AT]CC[CT] PstI 193 CTGCAG CTGCA^G SfuI 992 TTCGAA TT^CGAA SgrAI 89 CGCCGGCG C[GA]^CCGG[CT]G StuI 1125 AGGCCT AGG^CCT Tth111I 900 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 1149 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AfaI 729 GTAC GT^AC 1111 GTAC AluI 101 AGCT AG^CT 587 AGCT Alw44I 114 GTGCAC G^TGCAC 655 GTGCAC ApaLI 114 GTGCAC G^TGCAC 655 GTGCAC AspHI 114 GTGCAC G[AT]GC[AT]^C 655 GTGCAC BalI 830 TGGCCA TGG^CCA 1103 TGGCCA BbsI 777 GAAGAC GAAGAC(2/6) 1201 GAAGAC BpuAI 777 GAAGAC GAAGAC(2/6) 1201 GAAGAC BsaWI 419 TCCGGA [AT]^CCGG[AT] 953 ACCGGA BsiHKAI 114 GTGCAC G[AT]GC[AT]^C 655 GTGCAC BsrBI 387 GAGCGG CCGCTC(-3/-3) 524 GAGCGG BstXI 746 CCAAGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1320 CCAAATGACTGG MaeII 665 ACGT A^CGT 1134 ACGT MluNI 830 TGGCCA TGG^CCA 1103 TGGCCA MscI 830 TGGCCA TGG^CCA 1103 TGGCCA MspA1I 304 CCGCTG C[AC]G^C[GT]G 427 CCGCTG NcoI 770 CCATGG C^CATGG 827 CCATGG NlaIII 771 CATG CATG^ 828 CATG RsaI 729 GTAC GT^AC 1111 GTAC SfcI 147 CTACAG C^T[GA][CT]AG 193 CTGCAG SmaI 361 CCCGGG CCC^GGG 404 CCCGGG Tsp509I 179 AATT ^AATT 1289 AATT XmaCI 361 CCCGGG C^CCGGG 404 CCCGGG XmaI 361 CCCGGG C^CCGGG 404 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AccII AciI AcyI AlwI AvaI AvaII BanI BanII BbeI BbvI BcnI BmyI BpmI BsaHI BsaJI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssHII BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MaeIII MboI MboII MflI MnlI MspI MvaI MvnI MwoI NarI NciI NdeII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI SfoI StyI TaqI TseI Tsp45I TspRI TthHB8I XhoII