Restriction map for
pFN21AE2685
(Linear) (Six-base) MAPSORT of: KIEE2685.seq Check: 6870 from: 1 to: 1368
KIEE2685.orf
With 182 enzymes: SgfI *
June 2, 2017 11:42 ..
AflIII A'CryG_T
Cuts at: 0 1134 1368
Size: 1134 234
AlwNI CAG_nnn'CTG
Cuts at: 0 903 1368
Size: 903 465
ApaLI G'TGCA_C
Cuts at: 0 114 655 1368
Size: 114 541 713
Fragments arranged by size:
713 541 114
ApoI r'AATT_y
Cuts at: 0 1288 1368
Size: 1288 80
AvaI C'yCGr_G
Cuts at: 0 165 334 361 404 916 961 1215 1368
Size: 165 169 27 43 512 45 254 153
Fragments arranged by size:
512 254 169 165 153 45 43 27
BanI G'GyrC_C
Cuts at: 0 70 208 274 400 553 1368
Size: 70 138 66 126 153 815
Fragments arranged by size:
815 153 138 126 70 66
BanII G_rGCy'C
Cuts at: 0 172 224 235 341 1368
Size: 172 52 11 106 1027
Fragments arranged by size:
1027 172 106 52 11
BbeI G_GCGC'C
Cuts at: 0 74 212 278 404 557 1368
Size: 74 138 66 126 153 811
Fragments arranged by size:
811 153 138 126 74 66
BbsI GAAGACnn'nnnn_
Cuts at: 0 784 1208 1368
Size: 784 424 160
Fragments arranged by size:
784 424 160
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 636 670 1368
Size: 636 34 698
Fragments arranged by size:
698 636 34
BclI T'GATC_A
Cuts at: 0 446 1368
Size: 446 922
BglI GCCn_nnn'nGGC
Cuts at: 0 158 1368
Size: 158 1210
Bme1580I G_kGCm'C
Cuts at: 0 118 659 1215 1368
Size: 118 541 556 153
Fragments arranged by size:
556 541 153 118
BmgBI CAC'GTC
Cuts at: 0 666 1368
Size: 666 702
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 661 1002 1015 1368
Size: 661 341 13 353
Fragments arranged by size:
661 353 341 13
Bpu10I CC'TnA_GC
Cuts at: 0 80 1224 1368
Size: 80 1144 144
Fragments arranged by size:
1144 144 80
BsaHI Gr'CG_yC
Cuts at: 0 71 209 275 401 410 554 1368
Size: 71 138 66 126 9 144 814
Fragments arranged by size:
814 144 138 126 71 66 9
BsaWI w'CCGG_w
Cuts at: 0 419 953 1368
Size: 419 534 415
Fragments arranged by size:
534 419 415
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 11 1368
Size: 11 1357
BseYI C'CCAG_C
Cuts at: 0 374 436 1368
Size: 374 62 932
Fragments arranged by size:
932 374 62
BsiHKAI G_wGCw'C
Cuts at: 0 118 659 1368
Size: 118 541 709
Fragments arranged by size:
709 541 118
BsmI GAATG_Cn'
Cuts at: 0 818 1368
Size: 818 550
Bsp1286I G_dGCh'C
Cuts at: 0 118 172 224 235 341 659 1215 1368
Size: 118 54 52 11 106 318 556 153
Fragments arranged by size:
556 318 153 118 106 54 52 11
BspEI T'CCGG_A
Cuts at: 0 419 1368
Size: 419 949
BsrBI CCG'CTC
Cuts at: 0 389 526 1368
Size: 389 137 842
Fragments arranged by size:
842 389 137
BsrFI r'CCGG_y
Cuts at: 0 90 1368
Size: 90 1278
BssHII G'CGCG_C
Cuts at: 0 94 461 632 1368
Size: 94 367 171 736
Fragments arranged by size:
736 367 171 94
BstBI TT'CG_AA
Cuts at: 0 993 1368
Size: 993 375
BstXI CCAn_nnnn'nTGG
Cuts at: 0 753 1327 1368
Size: 753 574 41
Fragments arranged by size:
753 574 41
BstYI r'GATC_y
Cuts at: 0 507 920 1001 1052 1368
Size: 507 413 81 51 316
Fragments arranged by size:
507 413 316 81 51
BtgI C'CryG_G
Cuts at: 0 321 770 827 1368
Size: 321 449 57 541
Fragments arranged by size:
541 449 321 57
BtsI GCAGTG_nn'
Cuts at: 0 1215 1368
Size: 1215 153
ClaI AT'CG_AT
Cuts at: 0 971 1368
Size: 971 397
DrdI GACnn_nn'nnGTC
Cuts at: 0 1267 1368
Size: 1267 101
EaeI y'GGCC_r
Cuts at: 0 50 527 830 1103 1368
Size: 50 477 303 273 265
Fragments arranged by size:
477 303 273 265 50
EarI CTCTTCn'nnn_
Cuts at: 0 1349 1368
Size: 1349 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 14 20 26 410 1368
Size: 14 6 6 384 958
Fragments arranged by size:
958 384 14 6 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 661 1002 1015 1368
Size: 661 341 13 353
Fragments arranged by size:
661 353 341 13
EcoO109I rG'GnC_Cy
Cuts at: 0 626 1368
Size: 626 742
HaeII r_GCGC'y
Cuts at: 0 74 193 212 278 404 557 601 1368
Size: 74 119 19 66 126 153 44 767
Fragments arranged by size:
767 153 126 119 74 66 44 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 438 470 1224 1236 1256 1268 1368
Size: 438 32 754 12 20 12 100
Fragments arranged by size:
754 438 100 32 20 12 12
KasI G'GCGC_C
Cuts at: 0 70 208 274 400 553 1368
Size: 70 138 66 126 153 815
Fragments arranged by size:
815 153 138 126 70 66
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 701 1222 1368
Size: 701 521 146
Fragments arranged by size:
701 521 146
MscI TGG'CCA
Cuts at: 0 832 1105 1368
Size: 832 273 263
Fragments arranged by size:
832 273 263
MspA1I CmG'CkG
Cuts at: 0 306 429 1368
Size: 306 123 939
Fragments arranged by size:
939 306 123
NaeI GCC'GGC
Cuts at: 0 92 1368
Size: 92 1276
NarI GG'CG_CC
Cuts at: 0 71 209 275 401 554 1368
Size: 71 138 66 126 153 814
Fragments arranged by size:
814 153 138 126 71 66
NcoI C'CATG_G
Cuts at: 0 770 827 1368
Size: 770 57 541
Fragments arranged by size:
770 541 57
NgoMIV G'CCGG_C
Cuts at: 0 90 1368
Size: 90 1278
PfoI T'CCnGG_A
Cuts at: 0 979 1368
Size: 979 389
PpuMI rG'GwC_Cy
Cuts at: 0 626 1368
Size: 626 742
PstI C_TGCA'G
Cuts at: 0 197 1368
Size: 197 1171
SanDI GG'GwC_CC
Cuts at: 0 626 1368
Size: 626 742
SfcI C'TryA_G
Cuts at: 0 147 193 1368
Size: 147 46 1175
Fragments arranged by size:
1175 147 46
SfoI GGC'GCC
Cuts at: 0 72 210 276 402 555 1368
Size: 72 138 66 126 153 813
Fragments arranged by size:
813 153 138 126 72 66
SgrAI Cr'CCGG_yG
Cuts at: 0 90 1368
Size: 90 1278
SmaI CCC'GGG
Cuts at: 0 363 406 1368
Size: 363 43 962
Fragments arranged by size:
962 363 43
StuI AGG'CCT
Cuts at: 0 1127 1368
Size: 1127 241
StyI C'CwwG_G
Cuts at: 0 770 827 833 1172 1368
Size: 770 57 6 339 196
Fragments arranged by size:
770 339 196 57 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 382 1368
Size: 382 986
TatI w'GTAC_w
Cuts at: 0 1110 1368
Size: 1110 258
Tth111I GACn'n_nGTC
Cuts at: 0 903 1368
Size: 903 465
XmaI C'CCGG_G
Cuts at: 0 361 404 1368
Size: 361 43 964
Fragments arranged by size:
964 361 43
XmnI GAAnn'nnTTC
Cuts at: 0 1153 1368
Size: 1153 215
Enzymes that do cut:
AflIII AlwNI ApaLI ApoI AvaI BanI BanII BbeI
BbsI BcgI BclI BglI Bme1580I BmgBI BpmI Bpu10I
BsaHI BsaWI BseRI BseYI BsiHKAI BsmI Bsp1286I BspEI
BsrBI BsrFI BssHII BstBI BstXI BstYI BtgI BtsI
ClaI DrdI EaeI EarI EciI Eco57MI EcoO109I HaeII
Hin4I KasI MmeI MscI MspA1I NaeI NarI NcoI
NgoMIV PfoI PpuMI PstI SanDI SfcI SfoI SgrAI
SmaI StuI StyI TaqII TatI Tth111I XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI ApaI AscI AseI
AsiSI AvrII BaeI BamHI BbvCI BciVI BfrBI BglII
BlpI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI
BsaXI BsgI BsiEI BsiWI BsmBI BspHI BspMI BsrDI
BsrGI BssSI BstAPI BstEII BstZ17I Bsu36I BtgZI CspCI
DraI DraIII EagI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MslI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfiI SgfI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TstI
XbaI XcmI XhoI ZraI