Restriction map for
pFN21AB5904
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AcsI AflIII AgeI AhdI Alw44I
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BcnI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Cac8I CelII
Cfr10I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI FspI HgaI HindIII HpaI
KasI KpnI KspI MluI MroI
MspA1I MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 284 GACGTC GACGT^C
AcyI 284 GACGTC G[GA]^CG[CT]C
AflII 1311 CTTAAG C^TTAAG
AlwNI 1496 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 455 AGCGCT AGC^GCT
ApaI 1007 GGGCCC GGGCC^C
AspI 1337 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 1007 GGGCCC G[GA]GC[CT]^C
BbsI 727 GTCTTC GAAGAC(2/6)
BcgI 540 CGAGAGAGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 447 GGATAC GTATCC(6/5)
BfrI 1311 CTTAAG C^TTAAG
BpuAI 727 GTCTTC GAAGAC(2/6)
BsaBI 853 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 284 GACGTC G[GA]^CG[CT]C
BsaWI 423 TCCGGT [AT]^CCGG[AT]
BsgI 375 CTGCAC GTGCAG(16/14)
BspHI 1113 TCATGA T^CATGA
BstAPI 7 GCACTCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 456 GCGC GCG^C
DrdI 229 GACTGTTCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 393 CTCTTC CTCTTC(1/4)
Eco47III 455 AGCGCT AGC^GCT
HaeII 455 AGCGCT [GA]GCGC^[CT]
HhaI 456 GCGC GCG^C
Hin1I 284 GACGTC G[GA]^CG[CT]C
HinP1I 456 GCGC G^CGC
HincII 980 GTCAAC GT[CT]^[GA]AC
HindII 980 GTCAAC GT[CT]^[GA]AC
HphI 1399 GGTGA GGTGA(8/7)
Ksp632I 393 CTCTTC CTCTTC(1/4)
MamI 853 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 145 CAATTG C^AATTG
MunI 145 CAATTG C^AATTG
NspI 984 ACATGC [GA]CATG^[CT]
PflFI 1337 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1297 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1113 TCATGA T^CATGA
Tth111I 1337 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1297 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AlwI 844 GGATC GGATC(4/5)
1366 GGATC
AocI 736 CCTCAGG CC^T[ACGT]AGG
1417 CCTGAGG
BalI 404 TGGCCA TGG^CCA
1287 TGGCCA
BclI 29 TGATCA T^GATCA
110 TGATCA
BfaI 103 CTAG C^TAG
848 CTAG
BmyI 1007 GGGCCC G[AGT]GC[ACT]^C
1306 GGGCAC
BpmI 609 CTCCAG CTGGAG(16/14)
899 CTCCAG
BsmAI 391 GTCTC GTCTC(1/5)
1498 GTCTC
Bsp1286I 1007 GGGCCC G[AGT]GC[ACT]^C
1306 GGGCAC
BstYI 844 GGATCT [GA]^GATC[CT]
1366 GGATCT
Bsu36I 736 CCTCAGG CC^T[ACGT]AGG
1417 CCTGAGG
EaeI 404 TGGCCA [CT]^GGCC[GA]
1287 TGGCCA
Eco81I 736 CCTCAGG CC^T[ACGT]AGG
1417 CCTGAGG
FbaI 29 TGATCA T^GATCA
110 TGATCA
HapII 85 CCGG C^CGG
424 CCGG
HpaII 85 CCGG C^CGG
424 CCGG
MaeI 103 CTAG C^TAG
848 CTAG
MaeII 285 ACGT A^CGT
1017 ACGT
MflI 844 GGATCT [GA]^GATC[CT]
1366 GGATCT
MluNI 404 TGGCCA TGG^CCA
1287 TGGCCA
MscI 404 TGGCCA TGG^CCA
1287 TGGCCA
MseI 371 TTAA T^TAA
1312 TTAA
MspI 85 CCGG C^CGG
424 CCGG
PleI 1379 GACTC GAGTC(4/5)
57 GAGTC
PpuMI 350 AGGACCT [GA]G^G[AT]CC[CT]
1335 AGGACCC
PstI 69 CTGCAG CTGCA^G
651 CTGCAG
TaqI 539 TCGA T^CGA
731 TCGA
Tru9I 371 TTAA T^TAA
1312 TTAA
TthHB8I 539 TCGA T^CGA
731 TCGA
XbaI 102 TCTAGA T^CTAGA
847 TCTAGA
XhoII 844 GGATCT [GA]^GATC[CT]
1366 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BsaJI BseRI BsiYI BslI BsmFI
BsrDI BsrI BssKI BstF5I BstNI
BstXI Cfr13I DdeI DpnII DraII
DsaI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI ItaI MaeIII
MboI MboII MnlI MslI MvaI
MwoI NcoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TfiI TseI
Tsp45I Tsp509I TspRI