Restriction map for
pFN21AB5904
(Circular) (Six-base) MAPSORT of: pFN21AB5904.seq Check: 9667 from: 1 to: 6280
pFN21AB5904 6280 bp
With 182 enzymes: SgfI *
January 30, 2018 12:04 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2293 4090 278
Size: 53 83 186 936 757 1797 2468
Fragments arranged by size:
2468 1797 936 757 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3623 1937
Size: 1686 4594
Acc65I G'GTAC_C
Cuts at: 3601 3601
Size: 6280
AclI AA'CG_TT
Cuts at: 1563 4407 4780 1563
Size: 2844 373 3063
Fragments arranged by size:
3063 2844 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2385 4343 5727 5995 1632
Size: 753 1958 1384 268 1917
Fragments arranged by size:
1958 1917 1384 753 268
AfeI AGC'GCT
Cuts at: 2462 2462
Size: 6280
AflII C'TTAA_G
Cuts at: 820 1017 3316 820
Size: 197 2299 3784
Fragments arranged by size:
3784 2299 197
AflIII A'CryG_T
Cuts at: 1203 5179 6156 1203
Size: 3976 977 1327
Fragments arranged by size:
3976 1327 977
AgeI A'CCGG_T
Cuts at: 5111 5111
Size: 6280
AhdI GACnn_n'nnGTC
Cuts at: 5009 5009
Size: 6280
AleI CACnn'nnGTG
Cuts at: 1166 2799 1166
Size: 1633 4647
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6280
AlwNI CAG_nnn'CTG
Cuts at: 3506 4023 5595 3506
Size: 517 1572 4191
Fragments arranged by size:
4191 1572 517
ApaI G_GGCC'C
Cuts at: 3016 3016
Size: 6280
ApaLI G'TGCA_C
Cuts at: 4337 5493 4337
Size: 1156 5124
ApoI r'AATT_y
Cuts at: 1493 3589 3815 3967 6076 1493
Size: 2096 226 152 2109 1697
Fragments arranged by size:
2109 2096 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4834 160
Size: 872 3802 1606
Fragments arranged by size:
3802 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6280
AvaI C'yCGr_G
Cuts at: 1870 1943 3605 1870
Size: 73 1662 4545
Fragments arranged by size:
4545 1662 73
BamHI G'GATC_C
Cuts at: 3610 3977 3610
Size: 367 5913
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3601 5056 618
Size: 325 217 627 1814 1455 1842
Fragments arranged by size:
1842 1814 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3016 3599 1391
Size: 1625 583 4072
Fragments arranged by size:
4072 1625 583
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2725 928
Size: 971 826 4483
Fragments arranged by size:
4483 971 826
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2534 2568 4471 4505 1903
Size: 34 597 34 1903 34 3678
Fragments arranged by size:
3678 1903 597 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2446 4172 5382 2446
Size: 1726 1210 3344
Fragments arranged by size:
3344 1726 1210
BclI T'GATC_A
Cuts at: 1548 2034 2115 1548
Size: 486 81 5713
Fragments arranged by size:
5713 486 81
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4891 136
Size: 107 122 71 631 605 337 2882 1525
Fragments arranged by size:
2882 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6275 6275
Size: 6280
BlpI GC'TnA_GC
Cuts at: 3697 3697
Size: 6280
Bme1580I G_kGCm'C
Cuts at: 1790 3016 3315 4341 5497 1790
Size: 1226 299 1026 1156 2573
Fragments arranged by size:
2573 1226 1156 1026 299
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6280
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2806 4011 4969
Size: 416 53 83 399 24 1389 1205 958
Cuts at: 4969 442
Size: 1753
Fragments arranged by size:
1753 1389 1205 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6280
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2599 2889 4940 1239
Size: 1360 290 2051 2579
Fragments arranged by size:
2579 2051 1360 290
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2510 2962 4405 5270 5568 2510
Size: 452 1443 865 298 3222
Fragments arranged by size:
3222 1443 865 452 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4943 882
Size: 617 3444 2219
Fragments arranged by size:
3444 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4071 493
Size: 711 2867 2702
Fragments arranged by size:
2867 2702 711
BsaBI GATnn'nnATC
Cuts at: 2862 6274 2862
Size: 3412 2868
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2290 4087 4469 5104
Size: 53 83 186 936 757 1797 382 635
Cuts at: 5104 275
Size: 1451
Fragments arranged by size:
1797 1451 936 757 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2428 4713 5111 5385 5532 5943 2428
Size: 2285 398 274 147 411 2765
Fragments arranged by size:
2765 2285 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2815 2845 1178
Size: 30 165 30 1412 30 4613
Fragments arranged by size:
4613 1412 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2519 2580 2892 2943 3381 1186
Size: 1333 61 312 51 438 4085
Fragments arranged by size:
4085 1333 438 312 61 51
BseYI C'CCAG_C
Cuts at: 3437 5483 5979 3437
Size: 2046 496 3738
Fragments arranged by size:
3738 2046 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2365 1163
Size: 573 629 5078
Fragments arranged by size:
5078 629 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3730 4491 5519 1540
Size: 275 186 1729 761 1028 2301
Fragments arranged by size:
2301 1729 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4341 4426 5497 4341
Size: 85 1071 5124
Fragments arranged by size:
5124 1071 85
BsmI GAATG_Cn'
Cuts at: 3791 3884 3791
Size: 93 6187
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3016 3315 3599 4341 4426 5497 1391
Size: 399 1226 299 284 742 85 1071 2174
Fragments arranged by size:
2174 1226 1071 742 399 299 284 85
BspEI T'CCGG_A
Cuts at: 5943 5943
Size: 6280
BspHI T'CATG_A
Cuts at: 3118 4169 5899 3118
Size: 1051 1730 3499
Fragments arranged by size:
3499 1730 1051
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3635 844
Size: 2791 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4167 1394
Size: 429 2344 3507
Fragments arranged by size:
3507 2344 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2016 2039 3355 3446 4775 4949 65
Size: 1183 768 23 1316 91 1329 174 1396
Fragments arranged by size:
1396 1329 1316 1183 768 174 91 23
BsrFI r'CCGG_y
Cuts at: 1700 1810 4924 5111 1700
Size: 110 3114 187 2869
Fragments arranged by size:
3114 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6280
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6280
BssSI C'ACGA_G
Cuts at: 4340 5352 4340
Size: 1012 5268
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2018 794
Size: 1224 5056
BstBI TT'CG_AA
Cuts at: 5084 5084
Size: 6280
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6280
BstXI CCAn_nnnn'nTGG
Cuts at: 2801 3164 3179 3194 3209 3245 2801
Size: 363 15 15 15 36 5836
Fragments arranged by size:
5836 363 36 15 15 15
BstYI r'GATC_y
Cuts at: 1972 2849 3371 3610 3977 4362 4379 5831 5917
Size: 877 522 239 367 385 17 1452 86
Cuts at: 5917 5929 6275 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 877 522 385 367 346 239
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2742 3423 2742
Size: 681 5599
BtgI C'CryG_G
Cuts at: 513 1065 2845 3088 3148 3163 3193 3208 3289
Size: 552 1780 243 60 15 30 15 81
Cuts at: 3289 3295 513
Size: 6 3498
Fragments arranged by size:
3498 1780 552 243 81 60 30 15
15 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5541
Fragments arranged by size:
5541 683 56
BtsI GCAGTG_nn'
Cuts at: 2024 2083 3223 3796 4590 4610 2024
Size: 59 1140 573 794 20 3694
Fragments arranged by size:
3694 1140 794 573 59 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6280
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6245
DraI TTT'AAA
Cuts at: 3584 3584
Size: 6280
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2240 5287 809
Size: 507 924 3047 1802
Fragments arranged by size:
3047 1802 924 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2409 3292 3727
Size: 54 1425 50 275 18 579 883 435
Cuts at: 3727 4616 6245 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 883 579 435 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3727 1537
Size: 275 1915 4090
Fragments arranged by size:
4090 1915 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2404 4210 1353
Size: 1051 1806 3423
Fragments arranged by size:
3423 1806 1051
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4867 5241 5387 387
Size: 1064 138 3278 374 146 1280
Fragments arranged by size:
3278 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2385 2599 2889 4343 4940 5727 5995
Size: 393 753 214 290 1454 597 787 268
Cuts at: 5995 1239
Size: 1524
Fragments arranged by size:
1524 1454 787 753 597 393 290 268
214
EcoO109I rG'GnC_Cy
Cuts at: 2356 2948 3012 3341 2356
Size: 592 64 329 5295
Fragments arranged by size:
5295 592 329 64
EcoRI G'AATT_C
Cuts at: 3589 3967 3589
Size: 378 5902
FspI TGC'GCA
Cuts at: 4786 4786
Size: 6280
HaeII r_GCGC'y
Cuts at: 1678 2464 5427 1678
Size: 786 2963 2531
Fragments arranged by size:
2963 2531 786
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2507 2539 4921 4953 4995
Size: 32 135 32 935 32 2382 32 42
Cuts at: 4995 5027 1373
Size: 32 2626
Fragments arranged by size:
2626 2382 935 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2987 3624 3870 669
Size: 988 281 1049 637 246 3079
Fragments arranged by size:
3079 1049 988 637 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6280
HpaI GTT'AAC
Cuts at: 3870 3870
Size: 6280
KpnI G_GTAC'C
Cuts at: 3605 3605
Size: 6280
MfeI C'AATT_G
Cuts at: 2150 3879 2150
Size: 1729 4551
MluI A'CGCG_T
Cuts at: 6156 6156
Size: 6280
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5394 5578 1305
Size: 4089 184 2007
Fragments arranged by size:
4089 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2411 3294 10
Size: 54 1425 343 579 883 2996
Fragments arranged by size:
2996 1425 883 579 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2343 2640 2799 2886 2925 3072 3102
Size: 648 1177 297 159 87 39 147 30
Cuts at: 3102 3117 3123 3147 3162 3177 3192 3207 3228
Size: 15 6 24 15 15 15 15 21
Cuts at: 3228 4238 4597 4756 518
Size: 1010 359 159 2042
Fragments arranged by size:
2042 1177 1010 648 359 297 159 159
147 87 39 30 24 21 15 15
15 15 15 6
MspA1I CmG'CkG
Cuts at: 1606 3696 4373 5521 5766 1606
Size: 2090 677 1148 245 2120
Fragments arranged by size:
2120 2090 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2845 3148 3163 3193 3208 3289 513
Size: 552 1780 303 15 30 15 81 3504
Fragments arranged by size:
3504 1780 552 303 81 30 15 15
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6280
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6280
NotI GC'GGCC_GC
Cuts at: 3727 3727
Size: 6280
NspI r_CATG'y
Cuts at: 1132 1229 2993 3638 5183 1132
Size: 97 1764 645 1545 2229
Fragments arranged by size:
2229 1764 1545 645 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6280
PciI A'CATG_T
Cuts at: 5179 5179
Size: 6280
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3308 1108
Size: 2200 4080
PfoI T'CCnGG_A
Cuts at: 2249 2249
Size: 6280
PmeI GTTT'AAAC
Cuts at: 3584 3584
Size: 6280
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4349 4381 5862 5894 4349
Size: 32 1481 32 4735
Fragments arranged by size:
4735 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2356 3341 2356
Size: 985 5295
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6280
PsiI TTA'TAA
Cuts at: 3850 3850
Size: 6280
PspOMI G'GGCC_C
Cuts at: 3012 3012
Size: 6280
PstI C_TGCA'G
Cuts at: 830 2078 2660 3632 830
Size: 1248 582 972 3478
Fragments arranged by size:
3478 1248 972 582
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6280
SalI G'TCGA_C
Cuts at: 1936 3622 1936
Size: 1686 4594
SbfI CC_TGCA'GG
Cuts at: 3632 3632
Size: 6280
ScaI AGT'ACT
Cuts at: 4528 6240 4528
Size: 1712 4568
SfcI C'TryA_G
Cuts at: 826 1045 2074 2464 2656 3628 4763 5444 5635
Size: 219 1029 390 192 972 1135 681 191
Cuts at: 5635 826
Size: 1471
Fragments arranged by size:
1471 1135 1029 972 681 390 219 192
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6280
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6280
SmaI CCC'GGG
Cuts at: 1872 3607 1872
Size: 1735 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2489 2977 3316 4384 5285 5547
Size: 197 926 546 488 339 1068 901 262
Cuts at: 5547 820
Size: 1553
Fragments arranged by size:
1553 1068 926 901 546 488 339 262
197
SnaBI TAC'GTA
Cuts at: 493 4071 493
Size: 3578 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6280
SphI G_CATG'C
Cuts at: 1132 3638 1132
Size: 2506 3774
SspI AAT'ATT
Cuts at: 5 52 4204 5
Size: 47 4152 2081
Fragments arranged by size:
4152 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6280
StyI C'CwwG_G
Cuts at: 513 1065 2688 2845 3148 3163 3193 3208 3274
Size: 552 1623 157 303 15 30 15 66
Cuts at: 3274 3289 3719 513
Size: 15 430 3074
Fragments arranged by size:
3074 1623 552 430 303 157 66 30
15 15 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2230 2553 4307 4324 4477 4662 5107
Size: 1255 650 323 1754 17 153 185 445
Cuts at: 5107 325
Size: 1498
Fragments arranged by size:
1754 1498 1255 650 445 323 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2496 4526 6238
Size: 274 80 33 51 1443 519 2030 1712
Cuts at: 6238 96
Size: 138
Fragments arranged by size:
2030 1712 1443 519 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2254 2286 2815 2847 6240 6272 2254
Size: 32 529 32 3393 32 2262
Fragments arranged by size:
3393 2262 529 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3345 1316
Size: 2029 4251
XbaI T'CTAG_A
Cuts at: 2107 2852 3616 2107
Size: 745 764 4771
Fragments arranged by size:
4771 764 745
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6280
XmaI C'CCGG_G
Cuts at: 1870 3605 1870
Size: 1735 4545
XmnI GAAnn'nnTTC
Cuts at: 4409 4409
Size: 6280
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2291 4088 276
Size: 53 83 186 936 757 1797 2468
Fragments arranged by size:
2468 1797 936 757 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI