(Linear) (Six-base) MAPSORT of: KIBB5904.seq Check: 5800 from: 1 to: 1575 KIBB5904.orf With 182 enzymes: SgfI * January 30, 2018 10:51 .. AatII G_ACGT'C Cuts at: 0 288 1575 Size: 288 1287 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 380 1575 Size: 380 1195 AfeI AGC'GCT Cuts at: 0 457 1575 Size: 457 1118 AflII C'TTAA_G Cuts at: 0 1311 1575 Size: 1311 264 AleI CACnn'nnGTG Cuts at: 0 794 1575 Size: 794 781 AlwNI CAG_nnn'CTG Cuts at: 0 1501 1575 Size: 1501 74 ApaI G_GGCC'C Cuts at: 0 1011 1575 Size: 1011 564 BanII G_rGCy'C Cuts at: 0 1011 1575 Size: 1011 564 BbsI GAAGACnn'nnnn_ Cuts at: 0 720 1575 Size: 720 855 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 529 563 1575 Size: 529 34 1012 Fragments arranged by size: 1012 529 34 BciVI GTATCCnnnnn_n' Cuts at: 0 441 1575 Size: 441 1134 BclI T'GATC_A Cuts at: 0 29 110 1575 Size: 29 81 1465 Fragments arranged by size: 1465 81 29 Bme1580I G_kGCm'C Cuts at: 0 1011 1310 1575 Size: 1011 299 265 Fragments arranged by size: 1011 299 265 BmrI ACTGGGnnnn_n' Cuts at: 0 801 1575 Size: 801 774 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 594 884 1575 Size: 594 290 691 Fragments arranged by size: 691 594 290 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 505 957 1575 Size: 505 452 618 Fragments arranged by size: 618 505 452 BsaBI GATnn'nnATC Cuts at: 0 857 1575 Size: 857 718 BsaHI Gr'CG_yC Cuts at: 0 285 1575 Size: 285 1290 BsaWI w'CCGG_w Cuts at: 0 423 1575 Size: 423 1152 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 810 840 1575 Size: 810 30 735 Fragments arranged by size: 810 735 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 514 575 887 938 1376 1575 Size: 514 61 312 51 438 199 Fragments arranged by size: 514 438 312 199 61 51 BseYI C'CCAG_C Cuts at: 0 1432 1575 Size: 1432 143 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 360 1575 Size: 360 1215 Bsp1286I G_dGCh'C Cuts at: 0 1011 1310 1575 Size: 1011 299 265 Fragments arranged by size: 1011 299 265 BspHI T'CATG_A Cuts at: 0 1113 1575 Size: 1113 462 BsrDI GCAATG_nn' Cuts at: 0 11 34 1350 1441 1575 Size: 11 23 1316 91 134 Fragments arranged by size: 1316 134 91 23 11 BstAPI GCAn_nnn'nTGC Cuts at: 0 13 1575 Size: 13 1562 BstXI CCAn_nnnn'nTGG Cuts at: 0 796 1159 1174 1189 1204 1240 1575 Size: 796 363 15 15 15 36 335 Fragments arranged by size: 796 363 335 36 15 15 15 BstYI r'GATC_y Cuts at: 0 844 1366 1575 Size: 844 522 209 Fragments arranged by size: 844 522 209 Bsu36I CC'TnA_GG Cuts at: 0 737 1418 1575 Size: 737 681 157 Fragments arranged by size: 737 681 157 BtgI C'CryG_G Cuts at: 0 840 1083 1143 1158 1188 1203 1284 1290 Size: 840 243 60 15 30 15 81 6 Cuts at: 1290 1575 Size: 285 Fragments arranged by size: 840 285 243 81 60 30 15 15 6 BtsI GCAGTG_nn' Cuts at: 0 19 78 1218 1575 Size: 19 59 1140 357 Fragments arranged by size: 1140 357 59 19 DrdI GACnn_nn'nnGTC Cuts at: 0 235 1575 Size: 235 1340 EaeI y'GGCC_r Cuts at: 0 404 1287 1575 Size: 404 883 288 Fragments arranged by size: 883 404 288 EarI CTCTTCn'nnn_ Cuts at: 0 399 1575 Size: 399 1176 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 380 594 884 1575 Size: 380 214 290 691 Fragments arranged by size: 691 380 290 214 EcoO109I rG'GnC_Cy Cuts at: 0 351 943 1007 1336 1575 Size: 351 592 64 329 239 Fragments arranged by size: 592 351 329 239 64 HaeII r_GCGC'y Cuts at: 0 459 1575 Size: 459 1116 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 502 534 1575 Size: 502 32 1041 Fragments arranged by size: 1041 502 32 HincII GTy'rAC Cuts at: 0 982 1575 Size: 982 593 MfeI C'AATT_G Cuts at: 0 145 1575 Size: 145 1430 MscI TGG'CCA Cuts at: 0 406 1289 1575 Size: 406 883 286 Fragments arranged by size: 883 406 286 MslI CAynn'nnrTG Cuts at: 0 338 635 794 881 920 1067 1097 1112 Size: 338 297 159 87 39 147 30 15 Cuts at: 1112 1118 1142 1157 1172 1187 1202 1223 1575 Size: 6 24 15 15 15 15 21 352 Fragments arranged by size: 352 338 297 159 147 87 39 30 24 21 15 15 15 15 15 6 NcoI C'CATG_G Cuts at: 0 840 1143 1158 1188 1203 1284 1575 Size: 840 303 15 30 15 81 291 Fragments arranged by size: 840 303 291 81 30 15 15 NspI r_CATG'y Cuts at: 0 988 1575 Size: 988 587 PflMI CCAn_nnn'nTGG Cuts at: 0 1303 1575 Size: 1303 272 PfoI T'CCnGG_A Cuts at: 0 244 1575 Size: 244 1331 PpuMI rG'GwC_Cy Cuts at: 0 351 1336 1575 Size: 351 985 239 Fragments arranged by size: 985 351 239 PspOMI G'GGCC_C Cuts at: 0 1007 1575 Size: 1007 568 PstI C_TGCA'G Cuts at: 0 73 655 1575 Size: 73 582 920 Fragments arranged by size: 920 582 73 SfcI C'TryA_G Cuts at: 0 69 459 651 1575 Size: 69 390 192 924 Fragments arranged by size: 924 390 192 69 SmlI C'TyrA_G Cuts at: 0 484 972 1311 1575 Size: 484 488 339 264 Fragments arranged by size: 488 484 339 264 StyI C'CwwG_G Cuts at: 0 683 840 1143 1158 1188 1203 1269 1284 Size: 683 157 303 15 30 15 66 15 Cuts at: 1284 1575 Size: 291 Fragments arranged by size: 683 303 291 157 66 30 15 15 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 225 548 1575 Size: 225 323 1027 Fragments arranged by size: 1027 323 225 TatI w'GTAC_w Cuts at: 0 491 1575 Size: 491 1084 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 249 281 810 842 1575 Size: 249 32 529 32 733 Fragments arranged by size: 733 529 249 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1340 1575 Size: 1340 235 XbaI T'CTAG_A Cuts at: 0 102 847 1575 Size: 102 745 728 Fragments arranged by size: 745 728 102 ZraI GAC'GTC Cuts at: 0 286 1575 Size: 286 1289 Enzymes that do cut: AatII AcuI AfeI AflII AleI AlwNI ApaI BanII BbsI BcgI BciVI BclI Bme1580I BmrI BpmI BpuEI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI Bsp1286I BspHI BsrDI BstAPI BstXI BstYI Bsu36I BtgI BtsI DrdI EaeI EarI Eco57MI EcoO109I HaeII Hin4I HincII MfeI MscI MslI NcoI NspI PflMI PfoI PpuMI PspOMI PstI SfcI SmlI StyI TaqII TatI TstI Tth111I XbaI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AflIII AgeI AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BfrBI BglI BglII BlpI BmgBI BmtI BplI Bpu10I BsaI BsaAI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI KpnI MluI MmeI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI XcmI XhoI XmaI XmnI