(Circular) (Six-base) MAPSORT of: pFN21AE2678.seq Check: 9641 from: 1 to: 9157 pFN21AE2678 9157 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5825 6967 278 Size: 53 83 186 936 4289 1142 2468 Fragments arranged by size: 4289 2468 1142 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3573 5451 6500 1937 Size: 1636 1878 1049 4594 Fragments arranged by size: 4594 1878 1636 1049 Acc65I G'GTAC_C Cuts at: 2197 5581 6478 2197 Size: 3384 897 4876 Fragments arranged by size: 4876 3384 897 AclI AA'CG_TT Cuts at: 1563 4910 7284 7657 1563 Size: 3347 2374 373 3063 Fragments arranged by size: 3347 3063 2374 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3040 3219 3727 4864 4949 5291 5356 6205 Size: 1408 179 508 1137 85 342 65 849 Cuts at: 6205 7220 8604 8872 1632 Size: 1015 1384 268 1917 Fragments arranged by size: 1917 1408 1384 1137 1015 849 508 342 268 179 85 65 AfeI AGC'GCT Cuts at: 3670 5366 3670 Size: 1696 7461 AflII C'TTAA_G Cuts at: 820 1017 3398 820 Size: 197 2381 6579 Fragments arranged by size: 6579 2381 197 AflIII A'CryG_T Cuts at: 1203 2622 2766 4473 4941 6018 8056 9033 1203 Size: 1419 144 1707 468 1077 2038 977 1327 Fragments arranged by size: 2038 1707 1419 1327 1077 977 468 144 AgeI A'CCGG_T Cuts at: 5628 7988 5628 Size: 2360 6797 AhdI GACnn_n'nnGTC Cuts at: 2926 7886 2926 Size: 4960 4197 AleI CACnn'nnGTG Cuts at: 1166 5687 5924 1166 Size: 4521 237 4399 Fragments arranged by size: 4521 4399 237 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9157 AlwNI CAG_nnn'CTG Cuts at: 2813 3197 5791 6900 8472 2813 Size: 384 2594 1109 1572 3498 Fragments arranged by size: 3498 2594 1572 1109 384 ApaI G_GGCC'C Cuts at: 5520 6274 5520 Size: 754 8403 ApaLI G'TGCA_C Cuts at: 7214 8370 7214 Size: 1156 8001 ApoI r'AATT_y Cuts at: 1493 3046 3405 5126 6466 6692 6844 8953 1493 Size: 1553 359 1721 1340 226 152 2109 1697 Fragments arranged by size: 2109 1721 1697 1553 1340 359 226 152 AseI AT'TA_AT Cuts at: 160 1032 7711 160 Size: 872 6679 1606 Fragments arranged by size: 6679 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9157 AvaI C'yCGr_G Cuts at: 1870 1943 2202 5704 6482 1870 Size: 73 259 3502 778 4545 Fragments arranged by size: 4545 3502 778 259 73 BamHI G'GATC_C Cuts at: 6487 6854 6487 Size: 367 8790 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2197 3244 3765 5581 6478 Size: 325 217 627 410 1047 521 1816 897 Cuts at: 6478 7933 618 Size: 1455 1842 Fragments arranged by size: 1842 1816 1455 1047 897 627 521 410 325 217 BanII G_rGCy'C Cuts at: 1391 2083 2099 2393 2835 2955 3539 5013 5520 Size: 692 16 294 442 120 584 1474 507 Cuts at: 5520 5858 6008 6274 6476 1391 Size: 338 150 266 202 4072 Fragments arranged by size: 4072 1474 692 584 507 442 338 294 266 202 150 120 16 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4459 4579 928 Size: 971 2560 120 5506 Fragments arranged by size: 5506 2560 971 120 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5213 5247 7348 7382 1903 Size: 34 3276 34 2101 34 3678 Fragments arranged by size: 3678 3276 2101 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2791 7049 8259 2791 Size: 4258 1210 3689 Fragments arranged by size: 4258 3689 1210 BclI T'GATC_A Cuts at: 1548 3021 1548 Size: 1473 7684 BfrBI ATG'CAT Cuts at: 4111 4154 4111 Size: 43 9114 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2060 3527 5813 Size: 107 122 71 631 605 388 1467 2286 Cuts at: 5813 5954 7768 136 Size: 141 1814 1525 Fragments arranged by size: 2286 1814 1525 1467 631 605 388 141 122 107 71 BglII A'GATC_T Cuts at: 9152 9152 Size: 9157 BlpI GC'TnA_GC Cuts at: 6122 6574 6122 Size: 452 8705 Bme1580I G_kGCm'C Cuts at: 1790 2135 2781 3588 4065 5520 6075 6274 7218 Size: 345 646 807 477 1455 555 199 944 Cuts at: 7218 8374 1790 Size: 1156 2573 Fragments arranged by size: 2573 1455 1156 944 807 646 555 477 345 199 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9157 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2775 2838 5038 Size: 416 53 83 399 24 1358 63 2200 Cuts at: 5038 5449 5804 6888 7846 442 Size: 411 355 1084 958 1753 Fragments arranged by size: 2200 1753 1358 1084 958 416 411 399 355 83 63 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9157 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5794 5794 Size: 9157 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3257 6391 7817 1239 Size: 2018 3134 1426 2579 Fragments arranged by size: 3134 2579 2018 1426 Bpu10I CC'TnA_GC Cuts at: 2949 4001 2949 Size: 1052 8105 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2648 7282 8147 8445 2648 Size: 4634 865 298 3360 Fragments arranged by size: 4634 3360 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2222 4015 5538 6301 7820 882 Size: 617 723 1793 1523 763 1519 2219 Fragments arranged by size: 2219 1793 1523 1519 763 723 617 BsaAI yAC'GTr Cuts at: 493 1204 2769 5449 6948 493 Size: 711 1565 2680 1499 2702 Fragments arranged by size: 2702 2680 1565 1499 711 BsaBI GATnn'nnATC Cuts at: 3216 9151 3216 Size: 5935 3222 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5822 6245 6964 7346 Size: 53 83 186 936 4289 423 719 382 Cuts at: 7346 7981 275 Size: 635 1451 Fragments arranged by size: 4289 1451 936 719 635 423 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2277 5628 5724 5886 7590 7988 8262 8409 8820 Size: 3351 96 162 1704 398 274 147 411 Cuts at: 8820 2277 Size: 2614 Fragments arranged by size: 3351 2614 1704 411 398 274 162 147 96 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2174 2204 2375 2405 3104 Size: 30 165 30 771 30 171 30 699 Cuts at: 3104 3134 3492 3522 4631 4661 4673 4703 5615 Size: 30 358 30 1109 30 12 30 912 Cuts at: 5615 5645 5663 5693 1178 Size: 30 18 30 4642 Fragments arranged by size: 4642 1109 912 771 699 358 171 165 30 30 30 30 30 30 30 30 30 30 18 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2383 2618 3254 3362 4652 4883 5080 5526 Size: 1197 235 636 108 1290 231 197 446 Cuts at: 5526 1186 Size: 4817 Fragments arranged by size: 4817 1290 1197 636 446 235 231 197 108 BseYI C'CCAG_C Cuts at: 2102 2592 8360 8856 2102 Size: 490 5768 496 2403 Fragments arranged by size: 5768 2403 496 490 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3884 5571 5654 1163 Size: 573 2148 1687 83 4666 Fragments arranged by size: 4666 2148 1687 573 83 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3563 4635 5146 5342 6607 7368 Size: 275 186 1562 1072 511 196 1265 761 Cuts at: 7368 8396 1540 Size: 1028 2301 Fragments arranged by size: 2301 1562 1265 1072 1028 761 511 275 196 186 BsiHKAI G_wGCw'C Cuts at: 2083 2937 4815 5448 6047 7218 7303 8374 2083 Size: 854 1878 633 599 1171 85 1071 2866 Fragments arranged by size: 2866 1878 1171 1071 854 633 599 85 BsmI GAATG_Cn' Cuts at: 4831 6668 6761 4831 Size: 1837 93 7227 Fragments arranged by size: 7227 1837 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3715 3715 Size: 9157 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2083 2099 2135 2393 2781 2835 2937 Size: 399 293 16 36 258 388 54 102 Cuts at: 2937 2955 3539 3588 4065 4815 5013 5448 5520 Size: 18 584 49 477 750 198 435 72 Cuts at: 5520 5858 6008 6047 6075 6274 6476 7218 7303 Size: 338 150 39 28 199 202 742 85 Cuts at: 7303 8374 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 750 742 584 477 435 399 388 338 293 258 202 199 198 150 102 85 72 54 49 39 36 28 18 16 BspEI T'CCGG_A Cuts at: 8820 8820 Size: 9157 BspHI T'CATG_A Cuts at: 3510 3963 4428 7046 8776 3510 Size: 453 465 2618 1730 3891 Fragments arranged by size: 3891 2618 1730 465 453 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3296 4929 5593 5940 6512 844 Size: 2452 1633 664 347 572 3489 Fragments arranged by size: 3489 2452 1633 664 572 347 BsrBI CCG'CTC Cuts at: 1394 1823 2018 5175 5349 5476 7044 1394 Size: 429 195 3157 174 127 1568 3507 Fragments arranged by size: 3507 3157 1568 429 195 174 127 BsrDI GCAATG_nn' Cuts at: 65 1248 2112 3634 4154 4293 4546 5236 6418 Size: 1183 864 1522 520 139 253 690 1182 Cuts at: 6418 7652 7826 65 Size: 1234 174 1396 Fragments arranged by size: 1522 1396 1234 1183 1182 864 690 520 253 174 139 BsrFI r'CCGG_y Cuts at: 1700 1810 4971 5628 5800 5901 6289 7801 7988 Size: 110 3161 657 172 101 388 1512 187 Cuts at: 7988 1700 Size: 2869 Fragments arranged by size: 3161 2869 1512 657 388 187 172 110 101 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9157 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9157 BssSI C'ACGA_G Cuts at: 2126 2165 2978 7217 8229 2126 Size: 39 813 4239 1012 3054 Fragments arranged by size: 4239 3054 1012 813 39 BstAPI GCAn_nnn'nTGC Cuts at: 794 5646 794 Size: 4852 4305 BstBI TT'CG_AA Cuts at: 7961 7961 Size: 9157 BstEII G'GTnAC_C Cuts at: 1184 3445 4249 1184 Size: 2261 804 6092 Fragments arranged by size: 6092 2261 804 BstXI CCAn_nnnn'nTGG Cuts at: 2788 3895 3995 4726 4985 2788 Size: 1107 100 731 259 6960 Fragments arranged by size: 6960 1107 731 259 100 BstYI r'GATC_y Cuts at: 1972 2283 2334 2559 2649 6487 6854 7239 7256 Size: 311 51 225 90 3838 367 385 17 Cuts at: 7256 8708 8794 8806 9152 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 3838 1977 1452 385 367 346 311 225 90 86 51 17 12 BtgI C'CryG_G Cuts at: 513 1065 2193 2383 3717 4488 4621 6199 6231 Size: 552 1128 190 1334 771 133 1578 32 Cuts at: 6231 513 Size: 3439 Fragments arranged by size: 3439 1578 1334 1128 771 552 190 133 32 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2311 487 Size: 683 56 1085 7333 Fragments arranged by size: 7333 1085 683 56 BtsI GCAGTG_nn' Cuts at: 2907 3537 3942 4212 4267 4665 4996 6075 6673 Size: 630 405 270 55 398 331 1079 598 Cuts at: 6673 7467 7487 2907 Size: 794 20 4577 Fragments arranged by size: 4577 1079 794 630 598 405 398 331 270 55 20 ClaI AT'CG_AT Cuts at: 1554 5004 1554 Size: 3450 5707 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9122 DraI TTT'AAA Cuts at: 6461 6461 Size: 9157 DraIII CAC_nnn'GTG Cuts at: 5569 5569 Size: 9157 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8164 809 Size: 507 6848 1802 Fragments arranged by size: 6848 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2052 2061 2148 Size: 54 1425 50 275 18 222 9 87 Cuts at: 2148 2733 3933 4485 4716 5155 5289 6604 7493 Size: 585 1200 552 231 439 134 1315 889 Cuts at: 7493 9122 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1315 1200 889 585 552 439 275 231 222 134 87 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 6604 1537 Size: 275 4792 4090 Fragments arranged by size: 4792 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2087 2978 3195 3509 4883 7087 1353 Size: 734 891 217 314 1374 2204 3423 Fragments arranged by size: 3423 2204 1374 891 734 314 217 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7744 8118 8264 387 Size: 1064 138 6155 374 146 1280 Fragments arranged by size: 6155 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2081 2081 Size: 9157 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3040 3219 3257 3727 4864 4949 5291 Size: 393 1408 179 38 470 1137 85 342 Cuts at: 5291 5356 6205 6391 7220 7817 8604 8872 1239 Size: 65 849 186 829 597 787 268 1524 Fragments arranged by size: 1524 1408 1137 849 829 787 597 470 393 342 268 186 179 85 65 38 EcoNI CCTnn'n_nnAGG Cuts at: 3239 3292 5936 3239 Size: 53 2644 6460 Fragments arranged by size: 6460 2644 53 EcoO109I rG'GnC_Cy Cuts at: 2608 3519 5516 5517 6271 2608 Size: 911 1997 1 754 5494 Fragments arranged by size: 5494 1997 911 754 1 EcoRI G'AATT_C Cuts at: 6466 6844 6466 Size: 378 8779 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5386 5386 Size: 9157 FspI TGC'GCA Cuts at: 4712 7663 4712 Size: 2951 6206 HaeII r_GCGC'y Cuts at: 1678 3672 4327 4645 4767 5368 8304 1678 Size: 1994 655 318 122 601 2936 2531 Fragments arranged by size: 2936 2531 1994 655 601 318 122 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2255 2287 2408 2440 2641 Size: 32 135 32 683 32 121 32 201 Cuts at: 2641 2673 2912 2944 3492 3524 3545 3577 4631 Size: 32 239 32 548 32 21 32 1054 Cuts at: 4631 4663 4829 4861 5615 5647 7798 7830 7872 Size: 32 166 32 754 32 2151 32 42 Cuts at: 7872 7904 1373 Size: 32 2626 Fragments arranged by size: 2626 2151 1054 754 683 548 239 201 166 135 121 42 32 32 32 32 32 32 32 32 32 32 32 32 32 21 HincII GTy'rAC Cuts at: 669 1657 1938 3574 6501 6747 669 Size: 988 281 1636 2927 246 3079 Fragments arranged by size: 3079 2927 1636 988 281 246 HindIII A'AGCT_T Cuts at: 748 2463 748 Size: 1715 7442 HpaI GTT'AAC Cuts at: 6747 6747 Size: 9157 KpnI G_GTAC'C Cuts at: 2201 5585 6482 2201 Size: 3384 897 4876 Fragments arranged by size: 4876 3384 897 MfeI C'AATT_G Cuts at: 2451 6756 2451 Size: 4305 4852 MluI A'CGCG_T Cuts at: 9033 9033 Size: 9157 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2163 4567 4716 4804 6384 8271 8455 1305 Size: 858 2404 149 88 1580 1887 184 2007 Fragments arranged by size: 2404 2007 1887 1580 858 184 149 88 MscI TGG'CCA Cuts at: 10 64 1489 1832 2150 2735 3935 5157 10 Size: 54 1425 343 318 585 1200 1222 4010 Fragments arranged by size: 4010 1425 1222 1200 585 343 318 54 MslI CAynn'nnrTG Cuts at: 518 1166 2126 3530 5687 5924 6423 7115 7474 Size: 648 960 1404 2157 237 499 692 359 Cuts at: 7474 7633 518 Size: 159 2042 Fragments arranged by size: 2157 2042 1404 960 692 648 499 359 237 159 MspA1I CmG'CkG Cuts at: 1606 4441 4614 4927 5508 5577 5655 6035 6573 Size: 2835 173 313 581 69 78 380 538 Cuts at: 6573 7250 8398 8643 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2835 2120 1148 677 581 538 380 313 245 173 78 69 NaeI GCC'GGC Cuts at: 5802 5903 5802 Size: 101 9056 NcoI C'CATG_G Cuts at: 513 1065 4488 4621 6199 513 Size: 552 3423 133 1578 3471 Fragments arranged by size: 3471 3423 1578 552 133 NdeI CA'TA_TG Cuts at: 387 2848 387 Size: 2461 6696 NgoMIV G'CCGG_C Cuts at: 5800 5901 5800 Size: 101 9056 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9157 NotI GC'GGCC_GC Cuts at: 6604 6604 Size: 9157 NsiI A_TGCA'T Cuts at: 4113 4156 4113 Size: 43 9114 NspI r_CATG'y Cuts at: 1132 1229 2626 2926 4115 4477 6022 6121 6422 Size: 97 1397 300 1189 362 1545 99 301 Cuts at: 6422 6515 8060 1132 Size: 93 1545 2229 Fragments arranged by size: 2229 1545 1545 1397 1189 362 301 300 99 97 93 PasI CC'CwG_GG Cuts at: 1348 6390 1348 Size: 5042 4115 PciI A'CATG_T Cuts at: 2622 4473 6018 8056 2622 Size: 1851 1545 2038 3723 Fragments arranged by size: 3723 2038 1851 1545 PflMI CCAn_nnn'nTGG Cuts at: 1108 2040 2293 4627 1108 Size: 932 253 2334 5638 Fragments arranged by size: 5638 2334 932 253 PmeI GTTT'AAAC Cuts at: 6461 6461 Size: 9157 PmlI CAC'GTG Cuts at: 2769 2769 Size: 9157 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4895 4927 7226 7258 8739 8771 4895 Size: 32 2299 32 1481 32 5281 Fragments arranged by size: 5281 2299 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2608 2608 Size: 9157 PshAI GACnn'nnGTC Cuts at: 1613 4786 1613 Size: 3173 5984 PsiI TTA'TAA Cuts at: 3759 6727 3759 Size: 2968 6189 PspOMI G'GGCC_C Cuts at: 5516 6270 5516 Size: 754 8403 PstI C_TGCA'G Cuts at: 830 3159 4350 4926 5787 5937 6509 830 Size: 2329 1191 576 861 150 572 3478 Fragments arranged by size: 3478 2329 1191 861 576 572 150 PvuI CG_AT'CG Cuts at: 2001 3563 4635 2001 Size: 1562 1072 6523 Fragments arranged by size: 6523 1562 1072 PvuII CAG'CTG Cuts at: 4441 4614 4927 5655 6035 4441 Size: 173 313 728 380 7563 Fragments arranged by size: 7563 728 380 313 173 RsrII CG'GwC_CG Cuts at: 5631 5631 Size: 9157 SacI G_AGCT'C Cuts at: 2083 2083 Size: 9157 SalI G'TCGA_C Cuts at: 1936 3572 6499 1936 Size: 1636 2927 4594 Fragments arranged by size: 4594 2927 1636 SapI GCTCTTCn'nnn_ Cuts at: 2087 3195 2087 Size: 1108 8049 SbfI CC_TGCA'GG Cuts at: 5787 6509 5787 Size: 722 8435 ScaI AGT'ACT Cuts at: 3167 3740 7405 9117 3167 Size: 573 3665 1712 3207 Fragments arranged by size: 3665 3207 1712 573 SexAI A'CCwGG_T Cuts at: 3441 3987 3441 Size: 546 8611 SfcI C'TryA_G Cuts at: 826 1045 3155 4346 4609 4922 5149 5783 5933 Size: 219 2110 1191 263 313 227 634 150 Cuts at: 5933 6505 7640 8321 8512 826 Size: 572 1135 681 191 1471 Fragments arranged by size: 2110 1471 1191 1135 681 634 572 313 263 227 219 191 150 SfiI GGCCn_nnn'nGGCC Cuts at: 2060 2060 Size: 9157 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9157 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9157 SmaI CCC'GGG Cuts at: 1872 2204 6484 1872 Size: 332 4280 4545 Fragments arranged by size: 4545 4280 332 SmlI C'TyrA_G Cuts at: 820 1017 1943 2663 3398 7261 8162 8424 820 Size: 197 926 720 735 3863 901 262 1553 Fragments arranged by size: 3863 1553 926 901 735 720 262 197 SnaBI TAC'GTA Cuts at: 493 6948 493 Size: 6455 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9157 SphI G_CATG'C Cuts at: 1132 4115 6515 1132 Size: 2983 2400 3774 Fragments arranged by size: 3774 2983 2400 SspI AAT'ATT Cuts at: 5 52 4087 7081 5 Size: 47 4035 2994 2081 Fragments arranged by size: 4035 2994 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 9157 StyI C'CwwG_G Cuts at: 513 1065 2703 4488 4621 5591 5868 6199 6596 Size: 552 1638 1785 133 970 277 331 397 Cuts at: 6596 513 Size: 3074 Fragments arranged by size: 3074 1785 1638 970 552 397 331 277 133 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2246 2990 3670 4305 4547 5057 5474 Size: 1255 666 744 680 635 242 510 417 Cuts at: 5474 7184 7201 7354 7539 7984 325 Size: 1710 17 153 185 445 1498 Fragments arranged by size: 1710 1498 1255 744 680 666 635 510 445 417 242 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3165 3738 7403 Size: 274 80 33 51 1443 1188 573 3665 Cuts at: 7403 9115 96 Size: 1712 138 Fragments arranged by size: 3665 1712 1443 1188 573 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2174 2206 2375 2407 3104 3136 4029 4061 5663 Size: 32 169 32 697 32 893 32 1602 Cuts at: 5663 5695 6173 6205 9117 9149 2174 Size: 32 478 32 2912 32 2182 Fragments arranged by size: 2912 2182 1602 893 697 478 169 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5501 6146 1316 Size: 4185 645 4327 Fragments arranged by size: 4327 4185 645 XbaI T'CTAG_A Cuts at: 6493 6493 Size: 9157 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2041 4310 6289 2041 Size: 2269 1979 4909 Fragments arranged by size: 4909 2269 1979 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9157 XmaI C'CCGG_G Cuts at: 1870 2202 6482 1870 Size: 332 4280 4545 Fragments arranged by size: 4545 4280 332 XmnI GAAnn'nnTTC Cuts at: 4877 7286 4877 Size: 2409 6748 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5823 6965 276 Size: 53 83 186 936 4289 1142 2468 Fragments arranged by size: 4289 2468 1142 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI PacI PfoI PspXI PsrI SacII SanDI SfoI SrfI SwaI