################################## # NO CUT ENZYMES ( 61 enzymes) # ################################## AatII Acc65I AclI AflII AgeI AscI AseI AsnI Asp718I AviII AvrII BbrPI BciVI BclI BfaI BfrI BlnI BsaBI BsmI BspDI BssHII Bst1107I BstZ17I ClaI CpoI DraI EcoRI FbaI FseI FspI HpaI KpnI MaeI MamI MfeI MunI NdeI NheI NruI PacI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I RsrII SbfI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccIII 3777 TCCGGA T^CCGGA BamHI 2497 GGATCC G^GATCC BanI 3382 GGCGCC G^G[CT][GA]CC BbeI 3382 GGCGCC GGCGC^C BbvCI 3264 CCTCAGC CCTCAGC(-5/-2) BsaAI 1804 TACGTG [CT]AC^GT[GA] BsaI 1404 GGTCTC GGTCTC(1/5) BsaWI 3777 TCCGGA [AT]^CCGG[AT] BseAI 3777 TCCGGA T^CCGGA BsiWI 3848 CGTACG C^GTACG BspEI 3777 TCCGGA T^CCGGA BsrDI 3600 CATTGC GCAATG(2/0) BssSI 654 CACGAG CACGAG(-5/-1) BstBI 806 TTCGAA TT^CGAA BstEII 1216 GGTGACC G^GT[ACGT]ACC BstPI 1216 GGTGACC G^GT[ACGT]ACC DraIII 3715 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 1216 GGTGACC G^GT[ACGT]ACC EcoRV 733 GATATC GAT^ATC EcoT22I 197 ATGCAT ATGCA^T HincII 2641 GTCGAC GT[CT]^[GA]AC HindII 2641 GTCGAC GT[CT]^[GA]AC HindIII 271 AAGCTT A^AGCTT KasI 3382 GGCGCC G^GCGCC KspI 1592 CCGCGG CCGC^GG MluI 2066 ACGCGT A^CGCGT MroI 3777 TCCGGA T^CCGGA NarI 3382 GGCGCC GG^CGCC NcoI 2161 CCATGG C^CATGG NotI 949 GCGGCCGC GC^GGCCGC NsiI 197 ATGCAT ATGCA^T NspV 806 TTCGAA TT^CGAA PshAI 2359 GACGCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 716 CGATCG CGAT^CG SacII 1592 CCGCGG CCGC^GG SalI 2641 GTCGAC G^TCGAC SapI 1152 GCTCTTC GCTCTTC(1/4) ScaI 824 AGTACT AGT^ACT SexAI 3767 ACCTGGT A^CC[AT]GGT SfiI 2697 GGCCTGCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 3382 GGCGCC GGC^GCC SfuI 806 TTCGAA TT^CGAA ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AhdI 2230 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3889 GACGCCTGGTC AocI 2752 CCTGAGG CC^T[ACGT]AGG 3397 CCTCAGG Asp700I 447 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1986 GAAGATGTTC AspEI 2230 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3889 GACGCCTGGTC AspI 2635 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 3222 GACCAAGTC BalI 1645 TGGCCA TGG^CCA 1823 TGGCCA BcgI 3828 CGACAAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3357 GCAGACAAGTCG BglII 449 AGATCT A^GATCT 3543 AGATCT BlpI 2730 GCTCAGC GC^T[ACGT]AGC 2787 GCTCAGC Bpu1102I 2730 GCTCAGC GC^T[ACGT]AGC 2787 GCTCAGC Bsp1407I 1454 TGTACA T^GTACA 1607 TGTACA BspHI 263 TCATGA T^CATGA 1742 TCATGA BspLU11I 1610 ACATGT A^CATGT 1673 ACATGT BsrGI 1454 TGTACA T^GTACA 1607 TGTACA BstXI 1104 CCAGCCGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1636 CCAGGCATCTGG Bsu36I 2752 CCTGAGG CC^T[ACGT]AGG 3397 CCTCAGG CelII 2730 GCTCAGC GC^T[ACGT]AGC 2787 GCTCAGC DrdI 3072 GACAGCCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3126 GACTGCAGCGTC Eam1105I 2230 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3889 GACGCCTGGTC Eco81I 2752 CCTGAGG CC^T[ACGT]AGG 3397 CCTCAGG EcoNI 636 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2454 CCTGATGGAGG MluNI 1645 TGGCCA TGG^CCA 1823 TGGCCA MscI 1645 TGGCCA TGG^CCA 1823 TGGCCA NaeI 1958 GCCGGC GCC^GGC 3192 GCCGGC NgoMIV 1958 GCCGGC G^CCGGC 3192 GCCGGC PaeR7I 416 CTCGAG C^TCGAG 2172 CTCGAG PflFI 2635 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 3222 GACCAAGTC RcaI 263 TCATGA T^CATGA 1742 TCATGA SacI 370 GAGCTC GAGCT^C 988 GAGCTC SspBI 1454 TGTACA T^GTACA 1607 TGTACA Tth111I 2635 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 3222 GACCAAGTC XhoI 416 CTCGAG C^TCGAG 2172 CTCGAG XmnI 447 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1986 GAAGATGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI ApoI AspHI AvaI AvaII BanII BbsI BbvI BcnI BglI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco47III Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII