Restriction map for
pFN21AE3449
(Circular) (Six-base) MAPSORT of: pFN21AE3449.seq Check: 5823 from: 1 to: 8638
pFN21AE3449 8638 bp
With 182 enzymes: SgfI *
October 21, 2016 18:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2884 3456 2884
Size: 572 8066
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6448 278
Size: 53 83 186 936 4912 2468
Fragments arranged by size:
4912 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3360 3807 4647 5981 1937
Size: 1423 447 840 1334 4594
Fragments arranged by size:
4594 1423 1334 840 447
Acc65I G'GTAC_C
Cuts at: 5959 5959
Size: 8638
AclI AA'CG_TT
Cuts at: 1563 6765 7138 1563
Size: 5202 373 3063
Fragments arranged by size:
5202 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2471 3574 4890 5446 5678 6701 8085 8353
Size: 839 1103 1316 556 232 1023 1384 268
Cuts at: 8353 1632
Size: 1917
Fragments arranged by size:
1917 1384 1316 1103 1023 839 556 268
232
AfeI AGC'GCT
Cuts at: 3294 3632 5008 3294
Size: 338 1376 6924
Fragments arranged by size:
6924 1376 338
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8441
AflIII A'CryG_T
Cuts at: 1203 3615 3678 4071 7537 8514 1203
Size: 2412 63 393 3466 977 1327
Fragments arranged by size:
3466 2412 1327 977 393 63
AgeI A'CCGG_T
Cuts at: 7469 7469
Size: 8638
AhdI GACnn_n'nnGTC
Cuts at: 4240 5899 7367 4240
Size: 1659 1468 5511
Fragments arranged by size:
5511 1659 1468
AleI CACnn'nnGTG
Cuts at: 1166 4043 1166
Size: 2877 5761
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3494 1184
Size: 2310 6328
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5906 5938 5906
Size: 32 8606
AlwNI CAG_nnn'CTG
Cuts at: 4337 4418 4625 5358 5629 6381 7953 4337
Size: 81 207 733 271 752 1572 5022
Fragments arranged by size:
5022 1572 752 733 271 207 81
ApaI G_GGCC'C
Cuts at: 2749 3062 3442 4755 4779 4866 5704 2749
Size: 313 380 1313 24 87 838 5683
Fragments arranged by size:
5683 1313 838 380 313 87 24
ApaLI G'TGCA_C
Cuts at: 2933 3521 5825 6695 7851 2933
Size: 588 2304 870 1156 3720
Fragments arranged by size:
3720 2304 1156 870 588
ApoI r'AATT_y
Cuts at: 1493 2399 4049 4952 5947 6173 6325 8434 1493
Size: 906 1650 903 995 226 152 2109 1697
Fragments arranged by size:
2109 1697 1650 995 906 903 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7192 160
Size: 872 6160 1606
Fragments arranged by size:
6160 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8638
AvaI C'yCGr_G
Cuts at: 1870 1943 2421 3036 3373 4177 5963 1870
Size: 73 478 615 337 804 1786 4545
Fragments arranged by size:
4545 1786 804 615 478 337 73
BamHI G'GATC_C
Cuts at: 4502 5968 6335 4502
Size: 1466 367 6805
Fragments arranged by size:
6805 1466 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5387 5959 7414 618
Size: 325 217 627 3600 572 1455 1842
Fragments arranged by size:
3600 1842 1455 627 572 325 217
BanII G_rGCy'C
Cuts at: 1391 2379 2641 2749 2997 3062 3442 3705 4137
Size: 988 262 108 248 65 380 263 432
Cuts at: 4137 4551 4755 4779 4866 5067 5704 5924 5957
Size: 414 204 24 87 201 637 220 33
Cuts at: 5957 1391
Size: 4072
Fragments arranged by size:
4072 988 637 432 414 380 263 262
248 220 204 201 108 87 65 33
24
BbeI G_GCGC'C
Cuts at: 5391 5391
Size: 8638
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2857 4644 4896 928
Size: 971 958 1787 252 4670
Fragments arranged by size:
4670 1787 971 958 252
BbvCI CC'TCA_GC
Cuts at: 5270 5270
Size: 8638
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5351 5385 5822 5856 6829 6863 1903
Size: 34 3414 34 437 34 973 34 3678
Fragments arranged by size:
3678 3414 973 437 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6530 7740 6530
Size: 1210 7428
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8638
BfrBI ATG'CAT
Cuts at: 2204 2204
Size: 8638
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3066 3642 3980
Size: 107 122 71 631 605 1394 576 338
Cuts at: 3980 4709 5286 7249 136
Size: 729 577 1963 1525
Fragments arranged by size:
1963 1525 1394 729 631 605 577 576
338 122 107 71
BglII A'GATC_T
Cuts at: 2454 5548 8633 2454
Size: 3094 3085 2459
Fragments arranged by size:
3094 3085 2459
BlpI GC'TnA_GC
Cuts at: 4736 4793 6055 4736
Size: 57 1262 7319
Fragments arranged by size:
7319 1262 57
Bme1580I G_kGCm'C
Cuts at: 1790 2749 2937 3062 3351 3442 3525 4755 4779
Size: 959 188 125 289 91 83 1230 24
Cuts at: 4779 4866 5704 5829 6699 7855 1790
Size: 87 838 125 870 1156 2573
Fragments arranged by size:
2573 1230 1156 959 870 838 289 188
125 125 91 87 83 24
BmgBI CAC'GTC
Cuts at: 1318 2469 3922 1318
Size: 1151 1453 6034
Fragments arranged by size:
6034 1453 1151
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2117 6369 7327
Size: 416 53 83 399 24 700 4252 958
Cuts at: 7327 442
Size: 1753
Fragments arranged by size:
4252 1753 958 700 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8638
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3941 4783 3941
Size: 842 7796
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2641 3020 3376 4498 4702 5208 5560 7298
Size: 1402 379 356 1122 204 506 352 1738
Cuts at: 7298 1239
Size: 2579
Fragments arranged by size:
2579 1738 1402 1122 506 379 356 352
204
Bpu10I CC'TnA_GC
Cuts at: 4835 5270 4835
Size: 435 8203
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2160 2510 3155 5636 6763 7628 7926 2160
Size: 350 645 2481 1127 865 298 2872
Fragments arranged by size:
2872 2481 1127 865 645 350 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3415 7301 882
Size: 617 1916 3886 2219
Fragments arranged by size:
3886 2219 1916 617
BsaAI yAC'GTr
Cuts at: 493 1204 3811 6429 493
Size: 711 2607 2618 2702
Fragments arranged by size:
2702 2618 2607 711
BsaBI GATnn'nnATC
Cuts at: 8632 8632
Size: 8638
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3903 4365 5388 5753
Size: 53 83 186 936 2370 462 1023 365
Cuts at: 5753 5895 6445 6827 7462 275
Size: 142 550 382 635 1451
Fragments arranged by size:
2370 1451 1023 936 635 550 462 382
365 186 142 83 53
BsaWI w'CCGG_w
Cuts at: 5782 7071 7469 7743 7890 8301 5782
Size: 1289 398 274 147 411 6119
Fragments arranged by size:
6119 1289 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2900 2930 3167 3197 3374
Size: 30 165 30 1497 30 237 30 177
Cuts at: 3374 3395 3404 3425 4436 4466 5258 5288 5906
Size: 21 9 21 1011 30 792 30 618
Cuts at: 5906 5936 1178
Size: 30 3880
Fragments arranged by size:
3880 1497 1011 792 618 237 177 165
30 30 30 30 30 30 30 21
21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2348 2921 3172 3713 3793 4238 4872 5498
Size: 1162 573 251 541 80 445 634 626
Cuts at: 5498 5563 1186
Size: 65 4261
Fragments arranged by size:
4261 1162 634 626 573 541 445 251
80 65
BseYI C'CCAG_C
Cuts at: 3271 3423 4301 4920 5066 5104 5504 7841 8337
Size: 152 878 619 146 38 400 2337 496
Cuts at: 8337 3271
Size: 3572
Fragments arranged by size:
3572 2337 878 619 496 400 152 146
38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2501 3434 4364 4443 4595 4646 4862
Size: 573 765 933 930 79 152 51 216
Cuts at: 4862 5024 5413 5702 1163
Size: 162 389 289 4099
Fragments arranged by size:
4099 933 930 765 573 389 289 216
162 152 79 51
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2071 2724 2958 3964 5375 6088
Size: 275 186 70 653 234 1006 1411 713
Cuts at: 6088 6849 7877 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1411 1028 1006 761 713 653 275
234 186 70
BsiHKAI G_wGCw'C
Cuts at: 2379 2937 2997 3525 3927 4850 5829 6699 6784
Size: 558 60 528 402 923 979 870 85
Cuts at: 6784 7855 2379
Size: 1071 3162
Fragments arranged by size:
3162 1071 979 923 870 558 528 402
85 60
BsiWI C'GTAC_G
Cuts at: 5853 5853
Size: 8638
BsmI GAATG_Cn'
Cuts at: 6149 6242 6149
Size: 93 8545
BsmBI CGTCTCn'nnnn_
Cuts at: 3590 3912 4467 3590
Size: 322 555 7761
Fragments arranged by size:
7761 555 322
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2379 2641 2749 2937 2997 3062 3351
Size: 399 589 262 108 188 60 65 289
Cuts at: 3351 3442 3525 3705 3927 4137 4551 4755 4779
Size: 91 83 180 222 210 414 204 24
Cuts at: 4779 4850 4866 5067 5704 5829 5924 5957 6699
Size: 71 16 201 637 125 95 33 742
Cuts at: 6699 6784 7855 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 637 589 414 399 289
262 222 210 204 201 188 180 125
108 95 91 85 83 71 65 60
33 24 16
BspEI T'CCGG_A
Cuts at: 5782 8301 5782
Size: 2519 6119
BspHI T'CATG_A
Cuts at: 2268 3747 6527 8257 2268
Size: 1479 2780 1730 2649
Fragments arranged by size:
2780 2649 1730 1479
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2775 2884 3456 4740 5109 5993 844
Size: 1931 109 572 1284 369 884 3489
Fragments arranged by size:
3489 1931 1284 884 572 369 109
BsrBI CCG'CTC
Cuts at: 1394 1823 2911 3154 3783 5488 5689 6525 1394
Size: 429 1088 243 629 1705 201 836 3507
Fragments arranged by size:
3507 1705 1088 836 629 429 243 201
BsrDI GCAATG_nn'
Cuts at: 65 1248 5604 7133 7307 65
Size: 1183 4356 1529 174 1396
Fragments arranged by size:
4356 1529 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2493 3384 3963 4606 5197 7282 7469
Size: 110 683 891 579 643 591 2085 187
Cuts at: 7469 1700
Size: 2869
Fragments arranged by size:
2869 2085 891 683 643 591 579 187
110
BsrGI T'GTAC_A
Cuts at: 96 3459 3612 96
Size: 3363 153 5122
Fragments arranged by size:
5122 3363 153
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8638
BssSI C'ACGA_G
Cuts at: 2659 6698 7710 2659
Size: 4039 1012 3587
Fragments arranged by size:
4039 3587 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2488 2941 4430 5725 794
Size: 1694 453 1489 1295 3707
Fragments arranged by size:
3707 1694 1489 1295 453
BstBI TT'CG_AA
Cuts at: 2812 7442 2812
Size: 4630 4008
BstEII G'GTnAC_C
Cuts at: 1184 3221 1184
Size: 2037 6601
BstXI CCAn_nnnn'nTGG
Cuts at: 3116 3648 3116
Size: 532 8106
BstYI r'GATC_y
Cuts at: 1972 2454 4502 4786 5050 5548 5968 6335 6720
Size: 482 2048 284 264 498 420 367 385
Cuts at: 6720 6737 8189 8275 8287 8633 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2048 1977 1452 498 482 420 385 367
346 284 264 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4758 5403 4758
Size: 645 7993
BtgI C'CryG_G
Cuts at: 513 1065 3054 3597 3741 4166 4284 5757 513
Size: 552 1989 543 144 425 118 1473 3394
Fragments arranged by size:
3394 1989 1473 552 543 425 144 118
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2905 487
Size: 683 56 1679 6220
Fragments arranged by size:
6220 1679 683 56
BtsI GCAGTG_nn'
Cuts at: 2590 3076 3264 3523 5151 6154 6948 6968 2590
Size: 486 188 259 1628 1003 794 20 4260
Fragments arranged by size:
4260 1628 1003 794 486 259 188 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8638
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8603
DraI TTT'AAA
Cuts at: 5942 5942
Size: 8638
DraIII CAC_nnn'GTG
Cuts at: 5725 5725
Size: 8638
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5083 5137 7645 809
Size: 507 3767 54 2508 1802
Fragments arranged by size:
3767 2508 1802 507 54
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2068 2142 2284
Size: 54 1425 50 275 18 238 74 142
Cuts at: 2284 2955 3555 3650 3828 3961 5331 5372 5766
Size: 671 600 95 178 133 1370 41 394
Cuts at: 5766 5820 6085 6974 8603 8
Size: 54 265 889 1629 43
Fragments arranged by size:
1629 1425 1370 889 671 600 394 275
265 238 178 142 133 95 74 54
54 50 43 41 18
EagI C'GGCC_G
Cuts at: 1537 1812 2068 2955 3961 5372 6085 1537
Size: 275 256 887 1006 1411 713 4090
Fragments arranged by size:
4090 1411 1006 887 713 275 256
EarI CTCTTCn'nnn_
Cuts at: 1353 2223 2646 3164 3593 4395 4441 4949 5183
Size: 870 423 518 429 802 46 508 234
Cuts at: 5183 5939 6568 1353
Size: 756 629 3423
Fragments arranged by size:
3423 870 802 756 629 518 508 429
423 234 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3113 3710 5001 7225 7599 7745
Size: 1064 138 1524 597 1291 2224 374 146
Cuts at: 7745 387
Size: 1280
Fragments arranged by size:
2224 1524 1291 1280 1064 597 374 146
138
EcoICRI GAG'CTC
Cuts at: 2377 2995 2377
Size: 618 8020
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2471 2641 3020 3376 3574 4498 4702
Size: 393 839 170 379 356 198 924 204
Cuts at: 4702 4890 5208 5446 5560 5678 6701 7298 8085
Size: 188 318 238 114 118 1023 597 787
Cuts at: 8085 8353 1239
Size: 268 1524
Fragments arranged by size:
1524 1023 924 839 787 597 393 379
356 318 268 238 204 198 188 170
118 114
EcoNI CCTnn'n_nnAGG
Cuts at: 2645 4463 2645
Size: 1818 6820
EcoO109I rG'GnC_Cy
Cuts at: 2137 2426 2521 2746 3059 3378 3439 3792 4311
Size: 289 95 225 313 319 61 353 519
Cuts at: 4311 4711 4752 4775 4776 4801 4862 4863 5171
Size: 400 41 23 1 25 61 1 308
Cuts at: 5171 5626 5701 2137
Size: 455 75 5074
Fragments arranged by size:
5074 519 455 400 353 319 313 308
289 225 95 75 61 61 41 25
23 1 1
EcoRI G'AATT_C
Cuts at: 5947 6325 5947
Size: 378 8260
EcoRV GAT'ATC
Cuts at: 2740 2740
Size: 8638
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5893 5893
Size: 8638
FspI TGC'GCA
Cuts at: 7144 7144
Size: 8638
HaeII r_GCGC'y
Cuts at: 1678 2027 2628 3027 3095 3296 3634 5010 5031
Size: 349 601 399 68 201 338 1376 21
Cuts at: 5031 5391 7785 1678
Size: 360 2394 2531
Fragments arranged by size:
2531 2394 1376 601 399 360 349 338
201 68 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2900 2932 3167 3199 4226
Size: 32 135 32 1328 32 235 32 1027
Cuts at: 4226 4258 4325 4357 4436 4468 4494 4526 5218
Size: 32 67 32 79 32 26 32 692
Cuts at: 5218 5250 5258 5290 5540 5572 5885 5917 7279
Size: 32 8 32 250 32 313 32 1362
Cuts at: 7279 7311 7353 7385 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1362 1328 1027 692 313 250 235
135 79 67 42 32 32 32 32
32 32 32 32 32 32 32 32
32 32 26 8
HincII GTy'rAC
Cuts at: 669 1657 1938 4648 5982 6228 669
Size: 988 281 2710 1334 246 3079
Fragments arranged by size:
3079 2710 1334 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2276 748
Size: 1528 7110
HpaI GTT'AAC
Cuts at: 6228 6228
Size: 8638
KasI G'GCGC_C
Cuts at: 5387 5387
Size: 8638
KpnI G_GTAC'C
Cuts at: 5963 5963
Size: 8638
MfeI C'AATT_G
Cuts at: 6237 6237
Size: 8638
MluI A'CGCG_T
Cuts at: 4071 8514 4071
Size: 4443 4195
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2736 5883 7752 7936 1305
Size: 1431 3147 1869 184 2007
Fragments arranged by size:
3147 2007 1869 1431 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3652 3830 10
Size: 54 1425 343 1820 178 4818
Fragments arranged by size:
4818 1820 1425 343 178 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3731 3992 4043 6596 6955 7114 518
Size: 648 2565 261 51 2553 359 159 2042
Fragments arranged by size:
2565 2553 2042 648 359 261 159 51
MspA1I CmG'CkG
Cuts at: 1606 2392 2619 2941 3065 3599 3763 4688 4740
Size: 786 227 322 124 534 164 925 52
Cuts at: 4740 4807 4874 5223 5297 5748 6054 6731 7879
Size: 67 67 349 74 451 306 677 1148
Cuts at: 7879 8124 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 925 786 677 534 451 349
322 306 245 227 164 124 74 67
67 52
NaeI GCC'GGC
Cuts at: 3965 5199 3965
Size: 1234 7404
NarI GG'CG_CC
Cuts at: 5388 5388
Size: 8638
NcoI C'CATG_G
Cuts at: 513 1065 4166 513
Size: 552 3101 4985
Fragments arranged by size:
4985 3101 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8638
NgoMIV G'CCGG_C
Cuts at: 3963 5197 3963
Size: 1234 7404
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8638
NotI GC'GGCC_GC
Cuts at: 2955 6085 2955
Size: 3130 5508
NsiI A_TGCA'T
Cuts at: 2206 2206
Size: 8638
NspI r_CATG'y
Cuts at: 1132 1229 2491 3481 3619 3682 5996 7541 1132
Size: 97 1262 990 138 63 2314 1545 2229
Fragments arranged by size:
2314 2229 1545 1262 990 138 97 63
PasI CC'CwG_GG
Cuts at: 1348 2053 2749 3344 3898 4137 4533 4771 5383
Size: 705 696 595 554 239 396 238 612
Cuts at: 5383 1348
Size: 4603
Fragments arranged by size:
4603 705 696 612 595 554 396 239
238
PciI A'CATG_T
Cuts at: 3615 3678 7537 3615
Size: 63 3859 4716
Fragments arranged by size:
4716 3859 63
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8638
PfoI T'CCnGG_A
Cuts at: 2997 3126 3327 5185 5664 2997
Size: 129 201 1858 479 5971
Fragments arranged by size:
5971 1858 479 201 129
PmeI GTTT'AAAC
Cuts at: 5942 5942
Size: 8638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5906 5938 6707 6739 8220 8252 5906
Size: 32 769 32 1481 32 6292
Fragments arranged by size:
6292 1481 769 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2137 2521 3378 4311 4801 5626 2137
Size: 384 857 933 490 825 5149
Fragments arranged by size:
5149 933 857 825 490 384
PshAI GACnn'nnGTC
Cuts at: 1613 4368 1613
Size: 2755 5883
PsiI TTA'TAA
Cuts at: 6208 6208
Size: 8638
PspOMI G'GGCC_C
Cuts at: 2745 3058 3438 4751 4775 4862 5700 2745
Size: 313 380 1313 24 87 838 5683
Fragments arranged by size:
5683 1313 838 380 313 87 24
PspXI vC'TCGA_Gb
Cuts at: 4177 4177
Size: 8638
PstI C_TGCA'G
Cuts at: 830 2019 2317 2895 2946 3473 3791 3972 5027
Size: 1189 298 578 51 527 318 181 1055
Cuts at: 5027 5137 5990 830
Size: 110 853 3478
Fragments arranged by size:
3478 1189 1055 853 578 527 318 298
181 110 51
PvuI CG_AT'CG
Cuts at: 2001 2724 2001
Size: 723 7915
PvuII CAG'CTG
Cuts at: 2392 2619 2941 4688 4740 5223 5297 2392
Size: 227 322 1747 52 483 74 5733
Fragments arranged by size:
5733 1747 483 322 227 74 52
SacI G_AGCT'C
Cuts at: 2379 2997 2379
Size: 618 8020
SacII CC_GC'GG
Cuts at: 3600 3600
Size: 8638
SalI G'TCGA_C
Cuts at: 1936 4646 5980 1936
Size: 2710 1334 4594
Fragments arranged by size:
4594 2710 1334
SanDI GG'GwC_CC
Cuts at: 4311 4311
Size: 8638
SapI GCTCTTCn'nnn_
Cuts at: 3164 3164
Size: 8638
SbfI CC_TGCA'GG
Cuts at: 5990 5990
Size: 8638
ScaI AGT'ACT
Cuts at: 2831 6886 8598 2831
Size: 4055 1712 2871
Fragments arranged by size:
4055 2871 1712
SexAI A'CCwGG_T
Cuts at: 5772 5772
Size: 8638
SfcI C'TryA_G
Cuts at: 826 1045 2015 2313 2322 2614 2891 2942 3076
Size: 219 970 298 9 292 277 51 134
Cuts at: 3076 3469 3787 3968 5023 5133 5986 7121 7802
Size: 393 318 181 1055 110 853 1135 681
Cuts at: 7802 7993 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1055 970 853 681 393 318
298 292 277 219 191 181 134 110
51 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 4709 4709
Size: 8638
SfoI GGC'GCC
Cuts at: 5389 5389
Size: 8638
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8638
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8638
SmaI CCC'GGG
Cuts at: 1872 5965 1872
Size: 4093 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2175 2421 2525 3170 4177 5651
Size: 197 926 232 246 104 645 1007 1474
Cuts at: 5651 6742 7643 7905 820
Size: 1091 901 262 1553
Fragments arranged by size:
1553 1474 1091 1007 926 901 645 262
246 232 197 104
SnaBI TAC'GTA
Cuts at: 493 6429 493
Size: 5936 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8638
SphI G_CATG'C
Cuts at: 1132 5996 1132
Size: 4864 3774
SspI AAT'ATT
Cuts at: 5 52 6562 5
Size: 47 6510 2081
Fragments arranged by size:
6510 2081 47
StuI AGG'CCT
Cuts at: 1517 3087 4703 5443 1517
Size: 1570 1616 740 4712
Fragments arranged by size:
4712 1616 1570 740
StyI C'CwwG_G
Cuts at: 513 1065 2554 3186 4166 4440 4747 5260 5284
Size: 552 1489 632 980 274 307 513 24
Cuts at: 5284 5438 6077 513
Size: 154 639 3074
Fragments arranged by size:
3074 1489 980 639 632 552 513 307
274 154 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3357 5141 5518 6665 6682 6835 7020
Size: 1255 1777 1784 377 1147 17 153 185
Cuts at: 7020 7465 325
Size: 445 1498
Fragments arranged by size:
1784 1777 1498 1255 1147 445 377 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2829 2856 3459
Size: 274 80 33 51 1443 852 27 603
Cuts at: 3459 3612 3675 6884 8596 96
Size: 153 63 3209 1712 138
Fragments arranged by size:
3209 1712 1443 852 603 274 153 138
80 63 51 33 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3932 3964 4974 5006 8598 8630 3932
Size: 32 1010 32 3592 32 3940
Fragments arranged by size:
3940 3592 1010 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4643 5230 1316
Size: 3327 587 4724
Fragments arranged by size:
4724 3327 587
XbaI T'CTAG_A
Cuts at: 5974 5974
Size: 8638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2926 3645 4063 2926
Size: 719 418 7501
Fragments arranged by size:
7501 719 418
XhoI C'TCGA_G
Cuts at: 1943 2421 4177 1943
Size: 478 1756 6404
Fragments arranged by size:
6404 1756 478
XmaI C'CCGG_G
Cuts at: 1870 5963 1870
Size: 4093 4545
XmnI GAAnn'nnTTC
Cuts at: 2456 3995 6767 2456
Size: 1539 2772 4327
Fragments arranged by size:
4327 2772 1539
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6446 276
Size: 53 83 186 936 4912 2468
Fragments arranged by size:
4912 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BstZ17I FseI FspAI NruI PacI
PmlI PsrI RsrII SrfI SwaI