(Linear) (Six-base) MAPSORT of: KIEE3449.seq Check: 2377 from: 1 to: 3933 KIEE3449.orf With 182 enzymes: SgfI * October 21, 2016 17:13 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 879 1451 3933 Size: 879 572 2482 Fragments arranged by size: 2482 879 572 AccI GT'mk_AC Cuts at: 0 1355 1802 2642 3933 Size: 1355 447 840 1291 Fragments arranged by size: 1355 1291 840 447 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 466 1569 2885 3441 3673 3933 Size: 466 1103 1316 556 232 260 Fragments arranged by size: 1316 1103 556 466 260 232 AfeI AGC'GCT Cuts at: 0 1289 1627 3003 3933 Size: 1289 338 1376 930 Fragments arranged by size: 1376 1289 930 338 AflIII A'CryG_T Cuts at: 0 1610 1673 2066 3933 Size: 1610 63 393 1867 Fragments arranged by size: 1867 1610 393 63 AhdI GACnn_n'nnGTC Cuts at: 0 2235 3894 3933 Size: 2235 1659 39 Fragments arranged by size: 2235 1659 39 AleI CACnn'nnGTG Cuts at: 0 2038 3933 Size: 2038 1895 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1489 3933 Size: 1489 2444 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 3901 3933 Size: 3901 32 AlwNI CAG_nnn'CTG Cuts at: 0 2332 2413 2620 3353 3624 3933 Size: 2332 81 207 733 271 309 Fragments arranged by size: 2332 733 309 271 207 81 ApaI G_GGCC'C Cuts at: 0 744 1057 1437 2750 2774 2861 3699 3933 Size: 744 313 380 1313 24 87 838 234 Fragments arranged by size: 1313 838 744 380 313 234 87 24 ApaLI G'TGCA_C Cuts at: 0 928 1516 3820 3933 Size: 928 588 2304 113 Fragments arranged by size: 2304 928 588 113 ApoI r'AATT_y Cuts at: 0 394 2044 2947 3933 Size: 394 1650 903 986 Fragments arranged by size: 1650 986 903 394 AvaI C'yCGr_G Cuts at: 0 416 1031 1368 2172 3933 Size: 416 615 337 804 1761 Fragments arranged by size: 1761 804 615 416 337 BamHI G'GATC_C Cuts at: 0 2497 3933 Size: 2497 1436 BanI G'GyrC_C Cuts at: 0 3382 3933 Size: 3382 551 BanII G_rGCy'C Cuts at: 0 374 636 744 992 1057 1437 1700 2132 Size: 374 262 108 248 65 380 263 432 Cuts at: 2132 2546 2750 2774 2861 3062 3699 3919 3933 Size: 414 204 24 87 201 637 220 14 Fragments arranged by size: 637 432 414 380 374 263 262 248 220 204 201 108 87 65 24 14 BbeI G_GCGC'C Cuts at: 0 3386 3933 Size: 3386 547 BbsI GAAGACnn'nnnn_ Cuts at: 0 852 2639 2891 3933 Size: 852 1787 252 1042 Fragments arranged by size: 1787 1042 852 252 BbvCI CC'TCA_GC Cuts at: 0 3265 3933 Size: 3265 668 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3346 3380 3817 3851 3933 Size: 3346 34 437 34 82 Fragments arranged by size: 3346 437 82 34 34 BfrBI ATG'CAT Cuts at: 0 199 3933 Size: 199 3734 BglI GCCn_nnn'nGGC Cuts at: 0 1061 1637 1975 2704 3281 3933 Size: 1061 576 338 729 577 652 Fragments arranged by size: 1061 729 652 577 576 338 BglII A'GATC_T Cuts at: 0 449 3543 3933 Size: 449 3094 390 Fragments arranged by size: 3094 449 390 BlpI GC'TnA_GC Cuts at: 0 2731 2788 3933 Size: 2731 57 1145 Fragments arranged by size: 2731 1145 57 Bme1580I G_kGCm'C Cuts at: 0 744 932 1057 1346 1437 1520 2750 2774 Size: 744 188 125 289 91 83 1230 24 Cuts at: 2774 2861 3699 3824 3933 Size: 87 838 125 109 Fragments arranged by size: 1230 838 744 289 188 125 125 109 91 87 83 24 BmgBI CAC'GTC Cuts at: 0 464 1917 3933 Size: 464 1453 2016 Fragments arranged by size: 2016 1453 464 BmrI ACTGGGnnnn_n' Cuts at: 0 112 3933 Size: 112 3821 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1936 2778 3933 Size: 1936 842 1155 Fragments arranged by size: 1936 1155 842 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 636 1015 1371 2493 2697 3203 3555 3933 Size: 636 379 356 1122 204 506 352 378 Fragments arranged by size: 1122 636 506 379 378 356 352 204 Bpu10I CC'TnA_GC Cuts at: 0 2830 3265 3933 Size: 2830 435 668 Fragments arranged by size: 2830 668 435 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 155 505 1150 3631 3933 Size: 155 350 645 2481 302 Fragments arranged by size: 2481 645 350 302 155 BsaI GGTCTCn'nnnn_ Cuts at: 0 1410 3933 Size: 1410 2523 BsaAI yAC'GTr Cuts at: 0 1806 3933 Size: 1806 2127 BsaHI Gr'CG_yC Cuts at: 0 1898 2360 3383 3748 3890 3933 Size: 1898 462 1023 365 142 43 Fragments arranged by size: 1898 1023 462 365 142 43 BsaWI w'CCGG_w Cuts at: 0 3777 3933 Size: 3777 156 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 895 925 1162 1192 1369 1390 1399 1420 Size: 895 30 237 30 177 21 9 21 Cuts at: 1420 2431 2461 3253 3283 3901 3931 3933 Size: 1011 30 792 30 618 30 2 Fragments arranged by size: 1011 895 792 618 237 177 30 30 30 30 30 21 21 9 2 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 343 916 1167 1708 1788 2233 2867 3493 Size: 343 573 251 541 80 445 634 626 Cuts at: 3493 3558 3933 Size: 65 375 Fragments arranged by size: 634 626 573 541 445 375 343 251 80 65 BseYI C'CCAG_C Cuts at: 0 1266 1418 2296 2915 3061 3099 3499 3933 Size: 1266 152 878 619 146 38 400 434 Fragments arranged by size: 1266 878 619 434 400 152 146 38 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 496 1429 2359 2438 2590 2641 2857 3019 Size: 496 933 930 79 152 51 216 162 Cuts at: 3019 3408 3697 3933 Size: 389 289 236 Fragments arranged by size: 933 930 496 389 289 236 216 162 152 79 51 BsiEI CG_ry'CG Cuts at: 0 66 719 953 1959 3370 3933 Size: 66 653 234 1006 1411 563 Fragments arranged by size: 1411 1006 653 563 234 66 BsiHKAI G_wGCw'C Cuts at: 0 374 932 992 1520 1922 2845 3824 3933 Size: 374 558 60 528 402 923 979 109 Fragments arranged by size: 979 923 558 528 402 374 109 60 BsiWI C'GTAC_G Cuts at: 0 3848 3933 Size: 3848 85 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1585 1907 2462 3933 Size: 1585 322 555 1471 Fragments arranged by size: 1585 1471 555 322 Bsp1286I G_dGCh'C Cuts at: 0 374 636 744 932 992 1057 1346 1437 Size: 374 262 108 188 60 65 289 91 Cuts at: 1437 1520 1700 1922 2132 2546 2750 2774 2845 Size: 83 180 222 210 414 204 24 71 Cuts at: 2845 2861 3062 3699 3824 3919 3933 Size: 16 201 637 125 95 14 Fragments arranged by size: 637 414 374 289 262 222 210 204 201 188 180 125 108 95 91 83 71 65 60 24 16 14 BspEI T'CCGG_A Cuts at: 0 3777 3933 Size: 3777 156 BspHI T'CATG_A Cuts at: 0 263 1742 3933 Size: 263 1479 2191 Fragments arranged by size: 2191 1479 263 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 770 879 1451 2735 3104 3933 Size: 770 109 572 1284 369 829 Fragments arranged by size: 1284 829 770 572 369 109 BsrBI CCG'CTC Cuts at: 0 906 1149 1778 3483 3684 3933 Size: 906 243 629 1705 201 249 Fragments arranged by size: 1705 906 629 249 243 201 BsrDI GCAATG_nn' Cuts at: 0 3599 3933 Size: 3599 334 BsrFI r'CCGG_y Cuts at: 0 488 1379 1958 2601 3192 3933 Size: 488 891 579 643 591 741 Fragments arranged by size: 891 741 643 591 579 488 BsrGI T'GTAC_A Cuts at: 0 1454 1607 3933 Size: 1454 153 2326 Fragments arranged by size: 2326 1454 153 BssSI C'ACGA_G Cuts at: 0 654 3933 Size: 654 3279 BstAPI GCAn_nnn'nTGC Cuts at: 0 483 936 2425 3720 3933 Size: 483 453 1489 1295 213 Fragments arranged by size: 1489 1295 483 453 213 BstBI TT'CG_AA Cuts at: 0 807 3933 Size: 807 3126 BstEII G'GTnAC_C Cuts at: 0 1216 3933 Size: 1216 2717 BstXI CCAn_nnnn'nTGG Cuts at: 0 1111 1643 3933 Size: 1111 532 2290 Fragments arranged by size: 2290 1111 532 BstYI r'GATC_y Cuts at: 0 449 2497 2781 3045 3543 3933 Size: 449 2048 284 264 498 390 Fragments arranged by size: 2048 498 449 390 284 264 Bsu36I CC'TnA_GG Cuts at: 0 2753 3398 3933 Size: 2753 645 535 Fragments arranged by size: 2753 645 535 BtgI C'CryG_G Cuts at: 0 1049 1592 1736 2161 2279 3752 3933 Size: 1049 543 144 425 118 1473 181 Fragments arranged by size: 1473 1049 543 425 181 144 118 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 900 3933 Size: 900 3033 BtsI GCAGTG_nn' Cuts at: 0 585 1071 1259 1518 3146 3933 Size: 585 486 188 259 1628 787 Fragments arranged by size: 1628 787 585 486 259 188 DraIII CAC_nnn'GTG Cuts at: 0 3720 3933 Size: 3720 213 DrdI GACnn_nn'nnGTC Cuts at: 0 3078 3132 3933 Size: 3078 54 801 Fragments arranged by size: 3078 801 54 EaeI y'GGCC_r Cuts at: 0 63 137 279 950 1550 1645 1823 1956 Size: 63 74 142 671 600 95 178 133 Cuts at: 1956 3326 3367 3761 3815 3933 Size: 1370 41 394 54 118 Fragments arranged by size: 1370 671 600 394 178 142 133 118 95 74 63 54 41 EagI C'GGCC_G Cuts at: 0 63 950 1956 3367 3933 Size: 63 887 1006 1411 566 Fragments arranged by size: 1411 1006 887 566 63 EarI CTCTTCn'nnn_ Cuts at: 0 218 641 1159 1588 2390 2436 2944 3178 Size: 218 423 518 429 802 46 508 234 Cuts at: 3178 3933 Size: 755 Fragments arranged by size: 802 755 518 508 429 423 234 218 46 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1108 1705 2996 3933 Size: 1108 597 1291 937 Fragments arranged by size: 1291 1108 937 597 EcoICRI GAG'CTC Cuts at: 0 372 990 3933 Size: 372 618 2943 Fragments arranged by size: 2943 618 372 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 466 636 1015 1371 1569 2493 2697 2885 Size: 466 170 379 356 198 924 204 188 Cuts at: 2885 3203 3441 3555 3673 3933 Size: 318 238 114 118 260 Fragments arranged by size: 924 466 379 356 318 260 238 204 198 188 170 118 114 EcoNI CCTnn'n_nnAGG Cuts at: 0 640 2458 3933 Size: 640 1818 1475 Fragments arranged by size: 1818 1475 640 EcoO109I rG'GnC_Cy Cuts at: 0 132 421 516 741 1054 1373 1434 1787 Size: 132 289 95 225 313 319 61 353 Cuts at: 1787 2306 2706 2747 2770 2771 2796 2857 2858 Size: 519 400 41 23 1 25 61 1 Cuts at: 2858 3166 3621 3696 3933 Size: 308 455 75 237 Fragments arranged by size: 519 455 400 353 319 313 308 289 237 225 132 95 75 61 61 41 25 23 1 1 EcoRV GAT'ATC Cuts at: 0 735 3933 Size: 735 3198 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 3888 3933 Size: 3888 45 HaeII r_GCGC'y Cuts at: 0 22 623 1022 1090 1291 1629 3005 3026 Size: 22 601 399 68 201 338 1376 21 Cuts at: 3026 3386 3933 Size: 360 547 Fragments arranged by size: 1376 601 547 399 360 338 201 68 22 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 895 927 1162 1194 2221 2253 2320 2352 Size: 895 32 235 32 1027 32 67 32 Cuts at: 2352 2431 2463 2489 2521 3213 3245 3253 3285 Size: 79 32 26 32 692 32 8 32 Cuts at: 3285 3535 3567 3880 3912 3933 Size: 250 32 313 32 21 Fragments arranged by size: 1027 895 692 313 250 235 79 67 32 32 32 32 32 32 32 32 32 32 26 21 8 HincII GTy'rAC Cuts at: 0 2643 3933 Size: 2643 1290 HindIII A'AGCT_T Cuts at: 0 271 3933 Size: 271 3662 KasI G'GCGC_C Cuts at: 0 3382 3933 Size: 3382 551 MluI A'CGCG_T Cuts at: 0 2066 3933 Size: 2066 1867 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 731 3878 3933 Size: 731 3147 55 Fragments arranged by size: 3147 731 55 MscI TGG'CCA Cuts at: 0 1647 1825 3933 Size: 1647 178 2108 Fragments arranged by size: 2108 1647 178 MslI CAynn'nnrTG Cuts at: 0 1726 1987 2038 3933 Size: 1726 261 51 1895 Fragments arranged by size: 1895 1726 261 51 MspA1I CmG'CkG Cuts at: 0 387 614 936 1060 1594 1758 2683 2735 Size: 387 227 322 124 534 164 925 52 Cuts at: 2735 2802 2869 3218 3292 3743 3933 Size: 67 67 349 74 451 190 Fragments arranged by size: 925 534 451 387 349 322 227 190 164 124 74 67 67 52 NaeI GCC'GGC Cuts at: 0 1960 3194 3933 Size: 1960 1234 739 Fragments arranged by size: 1960 1234 739 NarI GG'CG_CC Cuts at: 0 3383 3933 Size: 3383 550 NcoI C'CATG_G Cuts at: 0 2161 3933 Size: 2161 1772 NgoMIV G'CCGG_C Cuts at: 0 1958 3192 3933 Size: 1958 1234 741 Fragments arranged by size: 1958 1234 741 NotI GC'GGCC_GC Cuts at: 0 950 3933 Size: 950 2983 NsiI A_TGCA'T Cuts at: 0 201 3933 Size: 201 3732 NspI r_CATG'y Cuts at: 0 486 1476 1614 1677 3933 Size: 486 990 138 63 2256 Fragments arranged by size: 2256 990 486 138 63 PasI CC'CwG_GG Cuts at: 0 48 744 1339 1893 2132 2528 2766 3378 Size: 48 696 595 554 239 396 238 612 Cuts at: 3378 3933 Size: 555 Fragments arranged by size: 696 612 595 555 554 396 239 238 48 PciI A'CATG_T Cuts at: 0 1610 1673 3933 Size: 1610 63 2260 Fragments arranged by size: 2260 1610 63 PfoI T'CCnGG_A Cuts at: 0 992 1121 1322 3180 3659 3933 Size: 992 129 201 1858 479 274 Fragments arranged by size: 1858 992 479 274 201 129 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 3901 3933 Size: 3901 32 PpuMI rG'GwC_Cy Cuts at: 0 132 516 1373 2306 2796 3621 3933 Size: 132 384 857 933 490 825 312 Fragments arranged by size: 933 857 825 490 384 312 132 PshAI GACnn'nnGTC Cuts at: 0 2363 3933 Size: 2363 1570 PspOMI G'GGCC_C Cuts at: 0 740 1053 1433 2746 2770 2857 3695 3933 Size: 740 313 380 1313 24 87 838 238 Fragments arranged by size: 1313 838 740 380 313 238 87 24 PspXI vC'TCGA_Gb Cuts at: 0 2172 3933 Size: 2172 1761 PstI C_TGCA'G Cuts at: 0 14 312 890 941 1468 1786 1967 3022 Size: 14 298 578 51 527 318 181 1055 Cuts at: 3022 3132 3933 Size: 110 801 Fragments arranged by size: 1055 801 578 527 318 298 181 110 51 14 PvuI CG_AT'CG Cuts at: 0 719 3933 Size: 719 3214 PvuII CAG'CTG Cuts at: 0 387 614 936 2683 2735 3218 3292 3933 Size: 387 227 322 1747 52 483 74 641 Fragments arranged by size: 1747 641 483 387 322 227 74 52 SacI G_AGCT'C Cuts at: 0 374 992 3933 Size: 374 618 2941 Fragments arranged by size: 2941 618 374 SacII CC_GC'GG Cuts at: 0 1595 3933 Size: 1595 2338 SalI G'TCGA_C Cuts at: 0 2641 3933 Size: 2641 1292 SanDI GG'GwC_CC Cuts at: 0 2306 3933 Size: 2306 1627 SapI GCTCTTCn'nnn_ Cuts at: 0 1159 3933 Size: 1159 2774 ScaI AGT'ACT Cuts at: 0 826 3933 Size: 826 3107 SexAI A'CCwGG_T Cuts at: 0 3767 3933 Size: 3767 166 SfcI C'TryA_G Cuts at: 0 10 308 317 609 886 937 1071 1464 Size: 10 298 9 292 277 51 134 393 Cuts at: 1464 1782 1963 3018 3128 3933 Size: 318 181 1055 110 805 Fragments arranged by size: 1055 805 393 318 298 292 277 181 134 110 51 10 9 SfiI GGCCn_nnn'nGGCC Cuts at: 0 2704 3933 Size: 2704 1229 SfoI GGC'GCC Cuts at: 0 3384 3933 Size: 3384 549 SmlI C'TyrA_G Cuts at: 0 170 416 520 1165 2172 3646 3933 Size: 170 246 104 645 1007 1474 287 Fragments arranged by size: 1474 1007 645 287 246 170 104 StuI AGG'CCT Cuts at: 0 1082 2698 3438 3933 Size: 1082 1616 740 495 Fragments arranged by size: 1616 1082 740 495 StyI C'CwwG_G Cuts at: 0 549 1181 2161 2435 2742 3255 3279 3433 Size: 549 632 980 274 307 513 24 154 Cuts at: 3433 3933 Size: 500 Fragments arranged by size: 980 632 549 513 500 307 274 154 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1352 3136 3513 3933 Size: 1352 1784 377 420 Fragments arranged by size: 1784 1352 420 377 TatI w'GTAC_w Cuts at: 0 824 851 1454 1607 1670 3933 Size: 824 27 603 153 63 2263 Fragments arranged by size: 2263 824 603 153 63 27 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1927 1959 2969 3001 3933 Size: 1927 32 1010 32 932 Fragments arranged by size: 1927 1010 932 32 32 Tth111I GACn'n_nGTC Cuts at: 0 2638 3225 3933 Size: 2638 587 708 Fragments arranged by size: 2638 708 587 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 921 1640 2058 3933 Size: 921 719 418 1875 Fragments arranged by size: 1875 921 719 418 XhoI C'TCGA_G Cuts at: 0 416 2172 3933 Size: 416 1756 1761 Fragments arranged by size: 1761 1756 416 XmnI GAAnn'nnTTC Cuts at: 0 451 1990 3933 Size: 451 1539 1943 Fragments arranged by size: 1943 1539 451 Enzymes that do cut: AarI AccI AcuI AfeI AflIII AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII KasI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NsiI NspI PasI PciI PfoI PpiI PpuMI PshAI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI ScaI SexAI SfcI SfiI SfoI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI XmnI Enzymes that do not cut: AatII Acc65I AclI AflII AgeI AscI AseI AsiSI AvrII BaeI BciVI BclI BmtI BsaBI BsmI BssHII BstZ17I ClaI CspCI DraI EcoRI FseI FspI FspAI HpaI KpnI MfeI NdeI NheI NruI PacI PflMI PmeI PmlI PsiI PsrI RsrII SbfI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI XbaI XmaI ZraI