################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AgeI AscI AseI AsnI Asp718I AspI AvrII BbeI BbrPI BglII BlnI BsaAI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BsrGI BssHII Bst1107I BstBI BstUI BstZ17I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco52I EcoT22I FseI HpaI KasI KpnI KspI MfeI MluI MroI MunI MvnI NarI NdeI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RsrII SacII SalI ScaI SexAI SfoI SfuI SgrAI SnaBI SpeI SspBI SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AclI 862 AACGTT AA^CGTT AflII 3052 CTTAAG C^TTAAG AflIII 1581 ACATGT A^C[GA][CT]GT AhdI 487 GACCTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 337 GTGCAC G^TGCAC Aor51HI 3659 AGCGCT AGC^GCT ApaLI 337 GTGCAC G^TGCAC Asp700I 1168 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 487 GACCTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 2737 TGCGCA TGC^GCA BamHI 260 GGATCC G^GATCC BanI 3575 GGCACC G^G[CT][GA]CC BciVI 3945 GGATAC GTATCC(6/5) BfrI 3052 CTTAAG C^TTAAG BsaBI 4128 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 3822 ACCGGA [AT]^CCGG[AT] BsmI 3057 GAATGC GAATGC(1/-1) BspHI 374 TCATGA T^CATGA BspLU11I 1581 ACATGT A^CATGT BsrDI 2538 GCAATG GCAATG(2/0) BssSI 1437 CACGAG CACGAG(-5/-1) BstYI 260 GGATCC [GA]^GATC[CT] Eam1105I 487 GACCTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 3659 AGCGCT AGC^GCT EcoRI 3475 GAATTC G^AATTC EcoRV 3841 GATATC GAT^ATC FspI 2737 TGCGCA TGC^GCA HincII 1258 GTCAAC GT[CT]^[GA]AC HindII 1258 GTCAAC GT[CT]^[GA]AC HindIII 1216 AAGCTT A^AGCTT MamI 4128 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 260 GGATCC [GA]^GATC[CT] NcoI 1118 CCATGG C^CATGG Psp1406I 862 AACGTT AA^CGTT RcaI 374 TCATGA T^CATGA SmaI 1322 CCCGGG CCC^GGG SphI 755 GCATGC GCATG^C XhoII 260 GGATCC [GA]^GATC[CT] XmaCI 1322 CCCGGG C^CCGGG XmaI 1322 CCCGGG C^CCGGG XmnI 1168 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcyI 492 GGCGTC G[GA]^CG[CT]C 2803 GACGCC AfaI 402 GTAC GT^AC 4048 GTAC BalI 233 TGGCCA TGG^CCA 523 TGGCCA BcgI 1659 CGAGCGGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3924 CGACCCAAATGC BclI 165 TGATCA T^GATCA 4040 TGATCA BsaHI 492 GGCGTC G[GA]^CG[CT]C 2803 GACGCC BsaI 1555 GAGACC GGTCTC(1/5) 1526 GGTCTC BsrBI 1660 GAGCGG CCGCTC(-3/-3) 3415 GAGCGG BstAPI 1419 GCAGGAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1917 GCAAGCCCTGC BstEII 909 GGTCACC G^GT[ACGT]ACC 2020 GGTGACC BstPI 909 GGTCACC G^GT[ACGT]ACC 2020 GGTGACC EcoO65I 909 GGTCACC G^GT[ACGT]ACC 2020 GGTGACC FbaI 165 TGATCA T^GATCA 4040 TGATCA Hin1I 492 GGCGTC G[GA]^CG[CT]C 2803 GACGCC MaeII 863 ACGT A^CGT 3629 ACGT MluNI 233 TGGCCA TGG^CCA 523 TGGCCA MscI 233 TGGCCA TGG^CCA 523 TGGCCA MseI 2510 TTAA T^TAA 3053 TTAA RsaI 402 GTAC GT^AC 4048 GTAC StuI 1748 AGGCCT AGG^CCT 4152 AGGCCT Tru9I 2510 TTAA T^TAA 3053 TTAA XcmI 1850 CCAACAGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2256 CCAACAGGGAGTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AciI AcsI AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaI AvaII BanII BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstXI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NaeI NciI NdeII NgoMIV NheI NlaIII NlaIV NspI PaeR7I PflMI PleI PpuMI PstI PvuII SacI SapI Sau3AI Sau96I SbfI ScrFI SfaNI SfcI SfiI SmlI Sse8387I StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoI