(Linear) (Six-base) MAPSORT of: KIEE2634.seq Check: 1786 from: 1 to: 4434 KIEE2634.orf With 182 enzymes: SgfI * August 9, 2016 17:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1635 1690 4434 Size: 1635 55 2744 Fragments arranged by size: 2744 1635 55 AclI AA'CG_TT Cuts at: 0 863 4434 Size: 863 3571 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1288 2509 2914 3166 4001 4042 4185 4434 Size: 1288 1221 405 252 835 41 143 249 Fragments arranged by size: 1288 1221 835 405 252 249 143 41 AfeI AGC'GCT Cuts at: 0 3661 4434 Size: 3661 773 AflII C'TTAA_G Cuts at: 0 3052 4434 Size: 3052 1382 AflIII A'CryG_T Cuts at: 0 1581 4434 Size: 1581 2853 AhdI GACnn_n'nnGTC Cuts at: 0 492 4434 Size: 492 3942 AleI CACnn'nnGTG Cuts at: 0 385 2018 4434 Size: 385 1633 2416 Fragments arranged by size: 2416 1633 385 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 139 640 3549 4348 4434 Size: 139 501 2909 799 86 Fragments arranged by size: 2909 799 501 139 86 AlwNI CAG_nnn'CTG Cuts at: 0 398 765 786 963 1551 1902 2133 2163 Size: 398 367 21 177 588 351 231 30 Cuts at: 2163 2487 2532 4434 Size: 324 45 1902 Fragments arranged by size: 1902 588 398 367 351 324 231 177 45 30 21 ApaI G_GGCC'C Cuts at: 0 2616 2967 3779 4434 Size: 2616 351 812 655 Fragments arranged by size: 2616 812 655 351 ApaLI G'TGCA_C Cuts at: 0 337 4434 Size: 337 4097 ApoI r'AATT_y Cuts at: 0 79 130 3475 4434 Size: 79 51 3345 959 Fragments arranged by size: 3345 959 79 51 AvaI C'yCGr_G Cuts at: 0 1322 1657 2197 3409 4434 Size: 1322 335 540 1212 1025 Fragments arranged by size: 1322 1212 1025 540 335 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 133 166 1796 1829 3862 3895 4434 Size: 133 33 1630 33 2033 33 539 Fragments arranged by size: 2033 1630 539 133 33 33 33 BamHI G'GATC_C Cuts at: 0 260 4434 Size: 260 4174 BanI G'GyrC_C Cuts at: 0 3575 4434 Size: 3575 859 BanII G_rGCy'C Cuts at: 0 290 442 893 1444 1841 1975 2196 2520 Size: 290 152 451 551 397 134 221 324 Cuts at: 2520 2616 2798 2967 2999 3746 3779 4434 Size: 96 182 169 32 747 33 655 Fragments arranged by size: 747 655 551 451 397 324 290 221 182 169 152 134 96 33 32 BbsI GAAGACnn'nnnn_ Cuts at: 0 3263 3989 4164 4203 4434 Size: 3263 726 175 39 231 Fragments arranged by size: 3263 726 231 175 39 BbvCI CC'TCA_GC Cuts at: 0 787 1636 2447 3125 3223 3497 4434 Size: 787 849 811 678 98 274 937 Fragments arranged by size: 937 849 811 787 678 274 98 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1648 1682 3913 3947 4434 Size: 1648 34 2231 34 487 Fragments arranged by size: 2231 1648 487 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 3939 4434 Size: 3939 495 BclI T'GATC_A Cuts at: 0 165 4040 4434 Size: 165 3875 394 Fragments arranged by size: 3875 394 165 BglI GCCn_nnn'nGGC Cuts at: 0 232 959 1927 1999 2065 2083 2584 2812 Size: 232 727 968 72 66 18 501 228 Cuts at: 2812 3086 3996 4434 Size: 274 910 438 Fragments arranged by size: 968 910 727 501 438 274 232 228 72 66 18 BlpI GC'TnA_GC Cuts at: 0 436 887 1124 2437 2675 2717 3178 4434 Size: 436 451 237 1313 238 42 461 1256 Fragments arranged by size: 1313 1256 461 451 436 238 237 42 Bme1580I G_kGCm'C Cuts at: 0 341 1278 2562 2616 2967 3779 4434 Size: 341 937 1284 54 351 812 655 Fragments arranged by size: 1284 937 812 655 351 341 54 BmrI ACTGGGnnnn_n' Cuts at: 0 219 1172 4434 Size: 219 953 3262 Fragments arranged by size: 3262 953 219 BmtI G_CTAG'C Cuts at: 0 1117 1462 2314 4434 Size: 1117 345 852 2120 Fragments arranged by size: 2120 1117 852 345 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2835 3900 4434 Size: 2835 1065 534 Fragments arranged by size: 2835 1065 534 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 13 579 877 985 1039 1348 1354 1531 Size: 13 566 298 108 54 309 6 177 Cuts at: 1531 1573 1615 1719 1882 1924 2008 2368 2686 Size: 42 42 104 163 42 84 360 318 Cuts at: 2686 2782 2860 4049 4434 Size: 96 78 1189 385 Fragments arranged by size: 1189 566 385 360 318 309 298 177 163 108 104 96 84 78 54 42 42 42 13 6 Bpu10I CC'TnA_GC Cuts at: 0 787 1636 2188 2369 2447 3125 3223 3433 Size: 787 849 552 181 78 678 98 210 Cuts at: 3433 3497 3634 3723 4434 Size: 64 137 89 711 Fragments arranged by size: 849 787 711 678 552 210 181 137 98 89 78 64 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 166 617 1072 2805 2992 3115 4291 4341 Size: 166 451 455 1733 187 123 1176 50 Cuts at: 4341 4434 Size: 93 Fragments arranged by size: 1733 1176 455 451 187 166 123 93 50 BsaI GGTCTCn'nnnn_ Cuts at: 0 1532 1549 4434 Size: 1532 17 2885 Fragments arranged by size: 2885 1532 17 BsaBI GATnn'nnATC Cuts at: 0 4132 4434 Size: 4132 302 BsaHI Gr'CG_yC Cuts at: 0 493 2804 4434 Size: 493 2311 1630 Fragments arranged by size: 2311 1630 493 BsaWI w'CCGG_w Cuts at: 0 3822 4434 Size: 3822 612 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1360 1390 1426 1456 1510 1540 1780 1810 Size: 1360 30 36 30 54 30 240 30 Cuts at: 1810 2107 2137 2614 2644 4434 Size: 297 30 477 30 1790 Fragments arranged by size: 1790 1360 477 297 240 54 36 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1003 1267 1384 1465 1468 1510 1612 1780 Size: 1003 264 117 81 3 42 102 168 Cuts at: 1780 1783 2155 2584 2614 2617 2638 2782 3112 Size: 3 372 429 30 3 21 144 330 Cuts at: 3112 3427 3514 3876 4167 4434 Size: 315 87 362 291 267 Fragments arranged by size: 1003 429 372 362 330 315 291 267 264 168 144 117 102 87 81 42 30 21 3 3 3 BseYI C'CCAG_C Cuts at: 0 1559 1669 2222 2306 2588 2721 2854 2939 Size: 1559 110 553 84 282 133 133 85 Cuts at: 2939 3745 4166 4434 Size: 806 421 268 Fragments arranged by size: 1559 806 553 421 282 268 133 133 110 85 84 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2446 2500 2677 2969 3168 3737 4434 Size: 2446 54 177 292 199 569 697 Fragments arranged by size: 2446 697 569 292 199 177 54 BsiHKAI G_wGCw'C Cuts at: 0 290 341 442 893 1444 1841 2468 2672 Size: 290 51 101 451 551 397 627 204 Cuts at: 2672 2798 2999 4434 Size: 126 201 1435 Fragments arranged by size: 1435 627 551 451 397 290 204 201 126 101 51 BsmI GAATG_Cn' Cuts at: 0 3063 4434 Size: 3063 1371 Bsp1286I G_dGCh'C Cuts at: 0 290 341 442 893 1278 1444 1841 1975 Size: 290 51 101 451 385 166 397 134 Cuts at: 1975 2196 2468 2520 2562 2616 2672 2798 2967 Size: 221 272 52 42 54 56 126 169 Cuts at: 2967 2999 3746 3779 4434 Size: 32 747 33 655 Fragments arranged by size: 747 655 451 397 385 290 272 221 169 166 134 126 101 56 54 52 51 42 33 32 BspHI T'CATG_A Cuts at: 0 374 4434 Size: 374 4060 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 632 1635 1690 4434 Size: 632 1003 55 2744 Fragments arranged by size: 2744 1003 632 55 BsrBI CCG'CTC Cuts at: 0 1662 3417 4434 Size: 1662 1755 1017 Fragments arranged by size: 1755 1662 1017 BsrDI GCAATG_nn' Cuts at: 0 2545 4434 Size: 2545 1889 BsrFI r'CCGG_y Cuts at: 0 1160 2914 3107 4434 Size: 1160 1754 193 1327 Fragments arranged by size: 1754 1327 1160 193 BssSI C'ACGA_G Cuts at: 0 1437 4434 Size: 1437 2997 BstAPI GCAn_nnn'nTGC Cuts at: 0 1425 1923 4434 Size: 1425 498 2511 Fragments arranged by size: 2511 1425 498 BstEII G'GTnAC_C Cuts at: 0 909 2020 4434 Size: 909 1111 2414 Fragments arranged by size: 2414 1111 909 BstXI CCAn_nnnn'nTGG Cuts at: 0 189 2854 4140 4434 Size: 189 2665 1286 294 Fragments arranged by size: 2665 1286 294 189 BstYI r'GATC_y Cuts at: 0 260 4434 Size: 260 4174 Bsu36I CC'TnA_GG Cuts at: 0 2458 3082 3971 4434 Size: 2458 624 889 463 Fragments arranged by size: 2458 889 624 463 BtgI C'CryG_G Cuts at: 0 1118 2747 3992 4434 Size: 1118 1629 1245 442 Fragments arranged by size: 1629 1245 1118 442 BtsI GCAGTG_nn' Cuts at: 0 373 845 907 2158 2560 2920 3531 3579 Size: 373 472 62 1251 402 360 611 48 Cuts at: 3579 3723 4434 Size: 144 711 Fragments arranged by size: 1251 711 611 472 402 373 360 144 62 48 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 3627 3662 4434 Size: 3627 35 772 Fragments arranged by size: 3627 772 35 EaeI y'GGCC_r Cuts at: 0 233 523 2900 3215 3594 4142 4434 Size: 233 290 2377 315 379 548 292 Fragments arranged by size: 2377 548 379 315 292 290 233 EarI CTCTTCn'nnn_ Cuts at: 0 306 564 1829 3062 4434 Size: 306 258 1265 1233 1372 Fragments arranged by size: 1372 1265 1233 306 258 EcoICRI GAG'CTC Cuts at: 0 288 440 891 1442 1839 2796 2997 4434 Size: 288 152 451 551 397 957 201 1437 Fragments arranged by size: 1437 957 551 451 397 288 201 152 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 13 579 877 985 1039 1288 1348 1354 Size: 13 566 298 108 54 249 60 6 Cuts at: 1354 1531 1573 1615 1719 1882 1924 2008 2368 Size: 177 42 42 104 163 42 84 360 Cuts at: 2368 2509 2686 2782 2860 2914 3166 4001 4042 Size: 141 177 96 78 54 252 835 41 Cuts at: 4042 4049 4185 4434 Size: 7 136 249 Fragments arranged by size: 835 566 360 298 252 249 249 177 177 163 141 136 108 104 96 84 78 60 54 54 42 42 42 41 13 7 6 EcoNI CCTnn'n_nnAGG Cuts at: 0 235 1345 1786 2320 2620 2977 4356 4434 Size: 235 1110 441 534 300 357 1379 78 Fragments arranged by size: 1379 1110 534 441 357 300 235 78 EcoO109I rG'GnC_Cy Cuts at: 0 960 1716 1779 2235 2613 2835 3269 3697 Size: 960 756 63 456 378 222 434 428 Cuts at: 3697 3776 4251 4434 Size: 79 475 183 Fragments arranged by size: 960 756 475 456 434 428 378 222 183 79 63 EcoRI G'AATT_C Cuts at: 0 3475 4434 Size: 3475 959 EcoRV GAT'ATC Cuts at: 0 3843 4434 Size: 3843 591 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2895 4434 Size: 2895 1539 FspI TGC'GCA Cuts at: 0 2739 4434 Size: 2739 1695 HaeII r_GCGC'y Cuts at: 0 1341 2847 3663 4434 Size: 1341 1506 816 771 Fragments arranged by size: 1506 1341 816 771 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 478 510 628 660 1129 1161 1360 1392 Size: 478 32 118 32 469 32 199 32 Cuts at: 1392 1510 1542 2507 2539 2614 2646 3937 3969 Size: 118 32 965 32 75 32 1291 32 Cuts at: 3969 4383 4415 4434 Size: 414 32 19 Fragments arranged by size: 1291 965 478 469 414 199 118 118 75 32 32 32 32 32 32 32 32 32 19 HincII GTy'rAC Cuts at: 0 1260 4434 Size: 1260 3174 HindIII A'AGCT_T Cuts at: 0 1216 4434 Size: 1216 3218 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 671 704 2379 4434 Size: 671 33 1675 2055 Fragments arranged by size: 2055 1675 671 33 MscI TGG'CCA Cuts at: 0 235 525 4434 Size: 235 290 3909 Fragments arranged by size: 3909 290 235 MslI CAynn'nnrTG Cuts at: 0 385 1586 2018 3991 4138 4330 4434 Size: 385 1201 432 1973 147 192 104 Fragments arranged by size: 1973 1201 432 385 192 147 104 MspA1I CmG'CkG Cuts at: 0 765 796 853 1290 1308 1542 1563 1953 Size: 765 31 57 437 18 234 21 390 Cuts at: 1953 2133 2226 2331 2487 2664 2691 2716 2961 Size: 180 93 105 156 177 27 25 245 Cuts at: 2961 3018 3114 3560 4166 4236 4434 Size: 57 96 446 606 70 198 Fragments arranged by size: 765 606 446 437 390 245 234 198 180 177 156 105 96 93 70 57 57 31 27 25 21 18 NaeI GCC'GGC Cuts at: 0 1162 2916 3109 4434 Size: 1162 1754 193 1325 Fragments arranged by size: 1754 1325 1162 193 NcoI C'CATG_G Cuts at: 0 1118 4434 Size: 1118 3316 NgoMIV G'CCGG_C Cuts at: 0 1160 2914 3107 4434 Size: 1160 1754 193 1327 Fragments arranged by size: 1754 1327 1160 193 NheI G'CTAG_C Cuts at: 0 1113 1458 2310 4434 Size: 1113 345 852 2124 Fragments arranged by size: 2124 1113 852 345 NspI r_CATG'y Cuts at: 0 759 953 1585 3990 4335 4434 Size: 759 194 632 2405 345 99 Fragments arranged by size: 2405 759 632 345 194 99 PciI A'CATG_T Cuts at: 0 1581 4434 Size: 1581 2853 PflMI CCAn_nnn'nTGG Cuts at: 0 348 398 1647 1902 2163 4166 4434 Size: 348 50 1249 255 261 2003 268 Fragments arranged by size: 2003 1249 348 268 261 255 50 PfoI T'CCnGG_A Cuts at: 0 1364 1550 1901 2525 2789 2798 4214 4434 Size: 1364 186 351 624 264 9 1416 220 Fragments arranged by size: 1416 1364 624 351 264 220 186 9 PpuMI rG'GwC_Cy Cuts at: 0 2235 3269 4251 4434 Size: 2235 1034 982 183 Fragments arranged by size: 2235 1034 982 183 PsiI TTA'TAA Cuts at: 0 140 4434 Size: 140 4294 PspOMI G'GGCC_C Cuts at: 0 2612 2963 3775 4434 Size: 2612 351 812 659 Fragments arranged by size: 2612 812 659 351 PspXI vC'TCGA_Gb Cuts at: 0 1657 4434 Size: 1657 2777 PstI C_TGCA'G Cuts at: 0 428 629 824 852 947 959 1034 1421 Size: 428 201 195 28 95 12 75 387 Cuts at: 1421 1547 1787 1928 2366 2567 2621 2864 2936 Size: 126 240 141 438 201 54 243 72 Cuts at: 2936 3119 3586 4434 Size: 183 467 848 Fragments arranged by size: 848 467 438 428 387 243 240 201 201 195 183 141 126 95 75 72 54 28 12 PvuII CAG'CTG Cuts at: 0 765 796 1290 1308 1542 1563 2133 2226 Size: 765 31 494 18 234 21 570 93 Cuts at: 2226 2331 2487 2664 2691 2716 3018 3114 3560 Size: 105 156 177 27 25 302 96 446 Cuts at: 3560 4166 4434 Size: 606 268 Fragments arranged by size: 765 606 570 494 446 302 268 234 177 156 105 96 93 31 27 25 21 18 SacI G_AGCT'C Cuts at: 0 290 442 893 1444 1841 2798 2999 4434 Size: 290 152 451 551 397 957 201 1435 Fragments arranged by size: 1435 957 551 451 397 290 201 152 SapI GCTCTTCn'nnn_ Cuts at: 0 306 564 1829 4434 Size: 306 258 1265 2605 Fragments arranged by size: 2605 1265 306 258 SbfI CC_TGCA'GG Cuts at: 0 959 1787 1928 2621 2936 4434 Size: 959 828 141 693 315 1498 Fragments arranged by size: 1498 959 828 693 315 141 SfcI C'TryA_G Cuts at: 0 424 625 820 848 943 955 1030 1417 Size: 424 201 195 28 95 12 75 387 Cuts at: 1417 1543 1783 1873 1924 2104 2362 2563 2617 Size: 126 240 90 51 180 258 201 54 Cuts at: 2617 2860 2932 3115 3582 3813 4119 4434 Size: 243 72 183 467 231 306 315 Fragments arranged by size: 467 424 387 315 306 258 243 240 231 201 201 195 183 180 126 95 90 75 72 54 51 28 12 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1999 2065 2584 4434 Size: 1999 66 519 1850 Fragments arranged by size: 1999 1850 519 66 SmaI CCC'GGG Cuts at: 0 1324 4434 Size: 1324 3110 SmlI C'TyrA_G Cuts at: 0 145 632 1051 1657 2197 2820 3007 3052 Size: 145 487 419 606 540 623 187 45 Cuts at: 3052 3094 3409 4306 4356 4434 Size: 42 315 897 50 78 Fragments arranged by size: 897 623 606 540 487 419 315 187 145 78 50 45 42 SphI G_CATG'C Cuts at: 0 759 4434 Size: 759 3675 SrfI GCCC'GGGC Cuts at: 0 1324 4434 Size: 1324 3110 StuI AGG'CCT Cuts at: 0 1750 4154 4434 Size: 1750 2404 280 Fragments arranged by size: 2404 1750 280 StyI C'CwwG_G Cuts at: 0 236 299 332 1037 1118 1244 1808 1976 Size: 236 63 33 705 81 126 564 168 Cuts at: 1976 2060 2903 3795 4434 Size: 84 843 892 639 Fragments arranged by size: 892 843 705 639 564 236 168 126 84 81 63 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 928 937 2008 2289 3169 3918 4434 Size: 928 9 1071 281 880 749 516 Fragments arranged by size: 1071 928 880 749 516 281 9 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1426 1458 2107 2139 4434 Size: 1426 32 649 32 2295 Fragments arranged by size: 2295 1426 649 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1857 2263 4434 Size: 1857 406 2171 Fragments arranged by size: 2171 1857 406 XhoI C'TCGA_G Cuts at: 0 1657 2197 3409 4434 Size: 1657 540 1212 1025 Fragments arranged by size: 1657 1212 1025 540 XmaI C'CCGG_G Cuts at: 0 1322 4434 Size: 1322 3112 XmnI GAAnn'nnTTC Cuts at: 0 1172 4434 Size: 1172 3262 Enzymes that do cut: AarI AclI AcuI AfeI AflII AflIII AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NheI NspI PciI PflMI PfoI PpuMI PsiI PspOMI PspXI PstI PvuII SacI SapI SbfI SfcI SfiI SmaI SmlI SphI SrfI StuI StyI TaqII TstI XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AgeI AloI AscI AseI AsiSI AvrII BbeI BfrBI BglII BmgBI BsaAI BsiEI BsiWI BsmBI BspEI BsrGI BssHII BstBI BstZ17I BtgZI ClaI DraI DraIII DrdI EagI EciI FseI FspAI HpaI KasI KpnI MfeI MluI NarI NdeI NotI NruI NsiI PacI PasI PmeI PmlI PpiI PshAI PsrI PvuI RsrII SacII SalI SanDI ScaI SexAI SfoI SgfI SgrAI SnaBI SpeI SspI SwaI TatI Tth111I XbaI ZraI