################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## AatII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AvrII BalI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmFI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HaeIII HapII Hin1I HpaI HpaII KasI KspI MamI MluI MluNI MroI MscI MspI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Van91I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 1194 GGTACC G^GTACC AccII 1450 CGCG CG^CG AlwNI 1505 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 1472 GAACTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1194 GGTACC G^GTACC AspHI 539 GAGCAC G[AT]GC[AT]^C AspI 1448 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 648 CTCGAG C^[CT]CG[GA]G BamHI 1726 GGATCC G^GATCC BanII 27 GGGCTC G[GA]GC[CT]^C BbsI 403 GAAGAC GAAGAC(2/6) BpuAI 403 GAAGAC GAAGAC(2/6) BsaAI 1688 CACGTA [CT]AC^GT[GA] BsgI 777 CTGCAC GTGCAG(16/14) BsiHKAI 539 GAGCAC G[AT]GC[AT]^C BsiYI 151 CCTCCTGTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 151 CCTCCTGTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsoBI 648 CTCGAG C^[CT]CG[GA]G BssSI 1879 CTCGTG CACGAG(-5/-1) BstUI 1450 CGCG CG^CG EcoNI 151 CCTCCTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 865 CCAAGG C^C[AT][AT]GG FauI 24 GCGGG CCCGC(4/6) HaeII 37 GGCGCT [GA]GCGC^[CT] HgaI 1448 GACGC GACGC(5/10) HincII 1744 GTTGAC GT[CT]^[GA]AC HindII 1744 GTTGAC GT[CT]^[GA]AC HindIII 828 AAGCTT A^AGCTT KpnI 1194 GGTACC GGTAC^C MfeI 323 CAATTG C^AATTG MslI 787 CATATTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 622 CAGCTG C[AC]G^C[GT]G MunI 323 CAATTG C^AATTG MvnI 1450 CGCG CG^CG NdeI 544 CATATG CA^TATG PaeR7I 648 CTCGAG C^TCGAG PflFI 1448 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 570 GAGTC GAGTC(4/5) PvuII 622 CAGCTG CAG^CTG SfcI 155 CTGTAG C^T[GA][CT]AG SspI 718 AATATT AAT^ATT StyI 865 CCAAGG C^C[AT][AT]GG Tth111I 1448 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1238 CCACTGTTCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 648 CTCGAG C^TCGAG XmnI 1472 GAACTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccI 1279 GTAGAC GT^[AC][GT]AC 1418 GTAGAC AcsI 838 GAATTT [GA]^AATT[CT] 1005 AAATTC ApoI 838 GAATTT [GA]^AATT[CT] 1005 AAATTC AvaII 1036 GGACC G^G[AT]CC 1453 GGTCC BanI 463 GGTGCC G^G[CT][GA]CC 1194 GGTACC BglI 111 GCCTGATAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 424 GCCAAAGAGGC BglII 337 AGATCT A^GATCT 681 AGATCT BpmI 1794 CTCCAG CTGGAG(16/14) 625 CTGGAG BsaJI 742 CCTGGG C^C[ACGT][ACGT]GG 865 CCAAGG BsmBI 1520 GAGACG CGTCTC(1/5) 1586 GAGACG BspMI 357 ACCTGC ACCTGC(4/8) 775 ACCTGC BssKI 742 CCTGG ^CC[ACGT]GG 1456 CCTGG BstNI 742 CCTGG CC^[AT]GG 1456 CCTGG Cfr13I 1036 GGACC G^G[ACGT]CC 1453 GGTCC DraI 448 TTTAAA TTT^AAA 1269 TTTAAA EcoRII 742 CCTGG ^CC[AT]GG 1456 CCTGG MvaI 742 CCTGG CC^[AT]GG 1456 CCTGG NspI 292 GCATGT [GA]CATG^[CT] 557 ACATGC Sau96I 1036 GGACC G^G[ACGT]CC 1453 GGTCC ScrFI 742 CCTGG CC^[ACGT]GG 1456 CCTGG SmlI 648 CTCGAG C^T[CT][GA]AG 1174 CTCAAG XbaI 762 TCTAGA T^CTAGA 1342 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AciI AfaI AluI AlwI AviII BbvI BfaI BmyI BseRI BsmAI Bsp1286I BspHI BsrI BstAPI BstF5I BstYI Cac8I CfoI DdeI DpnII EarI Fnu4HI FokI FspI HhaI HinP1I HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MwoI NdeII NlaIII NlaIV RcaI RsaI Sau3AI SfaNI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII