(Circular) (Six-base) MAPSORT of: pFN21AE2697.seq Check: 3147 from: 1 to: 6601 pFN21AE2697 6601 bp With 182 enzymes: SgfI * June 16, 2017 17:08 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4411 278 Size: 53 83 186 936 2875 2468 Fragments arranged by size: 2875 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3285 3424 3944 1937 Size: 1348 139 520 4594 Fragments arranged by size: 4594 1348 520 139 Acc65I G'GTAC_C Cuts at: 3199 3922 3199 Size: 723 5878 AclI AA'CG_TT Cuts at: 1563 4728 5101 1563 Size: 3165 373 3063 Fragments arranged by size: 3165 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2871 3456 3507 3722 4664 6048 6316 1632 Size: 1239 585 51 215 942 1384 268 1917 Fragments arranged by size: 1917 1384 1239 942 585 268 215 51 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6404 AflIII A'CryG_T Cuts at: 1203 5500 6477 1203 Size: 4297 977 1327 Fragments arranged by size: 4297 1327 977 AgeI A'CCGG_T Cuts at: 5432 5432 Size: 6601 AhdI GACnn_n'nnGTC Cuts at: 5330 5330 Size: 6601 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6601 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2442 1184 Size: 1258 5343 AlwNI CAG_nnn'CTG Cuts at: 3515 4344 5916 3515 Size: 829 1572 4200 Fragments arranged by size: 4200 1572 829 ApaLI G'TGCA_C Cuts at: 4658 5814 4658 Size: 1156 5445 ApoI r'AATT_y Cuts at: 1493 2843 3010 3910 4136 4288 6397 1493 Size: 1350 167 900 226 152 2109 1697 Fragments arranged by size: 2109 1697 1350 900 226 167 152 AseI AT'TA_AT Cuts at: 160 1032 5155 160 Size: 872 4123 1606 Fragments arranged by size: 4123 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6601 AvaI C'yCGr_G Cuts at: 1870 1943 2653 3926 1870 Size: 73 710 1273 4545 Fragments arranged by size: 4545 1273 710 73 BamHI G'GATC_C Cuts at: 3731 3931 4298 3731 Size: 200 367 6034 Fragments arranged by size: 6034 367 200 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2468 3199 3922 5377 618 Size: 325 217 627 681 731 723 1455 1842 Fragments arranged by size: 1842 1455 731 723 681 627 325 217 BanII G_rGCy'C Cuts at: 1391 2036 3920 1391 Size: 645 1884 4072 Fragments arranged by size: 4072 1884 645 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2415 928 Size: 971 516 5114 Fragments arranged by size: 5114 971 516 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4792 4826 1903 Size: 34 2855 34 3678 Fragments arranged by size: 3678 2855 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4493 5703 4493 Size: 1210 5391 BclI T'GATC_A Cuts at: 1548 1548 Size: 6601 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2122 2435 5212 Size: 107 122 71 631 605 450 313 2777 Cuts at: 5212 136 Size: 1525 Fragments arranged by size: 2777 1525 631 605 450 313 122 107 71 BglII A'GATC_T Cuts at: 2342 2686 6596 2342 Size: 344 3910 2347 Fragments arranged by size: 3910 2347 344 BlpI GC'TnA_GC Cuts at: 4018 4018 Size: 6601 Bme1580I G_kGCm'C Cuts at: 1790 3447 4662 5818 1790 Size: 1657 1215 1156 2573 Fragments arranged by size: 2573 1657 1215 1156 BmgBI CAC'GTC Cuts at: 1318 3641 1318 Size: 2323 4278 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4332 5290 442 Size: 416 53 83 399 24 2915 958 1753 Fragments arranged by size: 2915 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6601 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3548 3548 Size: 6601 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2651 3784 5261 1239 Size: 1412 1133 1477 2579 Fragments arranged by size: 2579 1477 1412 1133 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3164 4726 5591 5889 3164 Size: 1562 865 298 3876 Fragments arranged by size: 3876 1562 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5264 882 Size: 617 3765 2219 Fragments arranged by size: 3765 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3695 4392 493 Size: 711 2491 697 2702 Fragments arranged by size: 2702 2491 711 697 BsaBI GATnn'nnATC Cuts at: 6595 6595 Size: 6601 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4408 4790 5425 275 Size: 53 83 186 936 2875 382 635 1451 Fragments arranged by size: 2875 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5034 5432 5706 5853 6264 5034 Size: 398 274 147 411 5371 Fragments arranged by size: 5371 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3656 3686 3885 3915 1178 Size: 30 165 30 2253 30 199 30 3864 Fragments arranged by size: 3864 2253 199 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 3603 3660 1186 Size: 838 1579 57 4127 Fragments arranged by size: 4127 1579 838 57 BseYI C'CCAG_C Cuts at: 5804 6300 5804 Size: 496 6105 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2767 1163 Size: 573 1031 4997 Fragments arranged by size: 4997 1031 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4051 4812 5840 1540 Size: 275 186 2050 761 1028 2301 Fragments arranged by size: 2301 2050 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2548 4662 4747 5818 2548 Size: 2114 85 1071 3331 Fragments arranged by size: 3331 2114 1071 85 BsmI GAATG_Cn' Cuts at: 4112 4205 4112 Size: 93 6508 BsmBI CGTCTCn'nnnn_ Cuts at: 3519 3585 3519 Size: 66 6535 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 2548 3447 3920 4662 4747 5818 Size: 399 246 512 899 473 742 85 1071 Cuts at: 5818 1391 Size: 2174 Fragments arranged by size: 2174 1071 899 742 512 473 399 246 85 BspEI T'CCGG_A Cuts at: 6264 6264 Size: 6601 BspHI T'CATG_A Cuts at: 2074 2137 2662 4490 6220 2074 Size: 63 525 1828 1730 2455 Fragments arranged by size: 2455 1828 1730 525 63 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2371 2789 3956 844 Size: 1527 418 1167 3489 Fragments arranged by size: 3489 1527 1167 418 BsrBI CCG'CTC Cuts at: 1394 1823 4488 1394 Size: 429 2665 3507 Fragments arranged by size: 3507 2665 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5096 5270 65 Size: 1183 3848 174 1396 Fragments arranged by size: 3848 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5245 5432 1700 Size: 110 3435 187 2869 Fragments arranged by size: 3435 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6601 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6601 BssSI C'ACGA_G Cuts at: 3884 4661 5673 3884 Size: 777 1012 4812 Fragments arranged by size: 4812 1012 777 BstAPI GCAn_nnn'nTGC Cuts at: 794 2303 2468 3497 794 Size: 1509 165 1029 3898 Fragments arranged by size: 3898 1509 1029 165 BstBI TT'CG_AA Cuts at: 5405 5405 Size: 6601 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6601 BstYI r'GATC_y Cuts at: 1972 2342 2686 3731 3931 4298 4683 4700 6152 Size: 370 344 1045 200 367 385 17 1452 Cuts at: 6152 6238 6250 6596 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1045 385 370 367 346 344 200 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5110 Fragments arranged by size: 5110 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3217 487 Size: 683 56 1991 3871 Fragments arranged by size: 3871 1991 683 56 BtsI GCAGTG_nn' Cuts at: 2754 2823 4117 4911 4931 2754 Size: 69 1294 794 20 4424 Fragments arranged by size: 4424 1294 794 69 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6601 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6566 DraI TTT'AAA Cuts at: 2455 3276 3905 2455 Size: 821 629 5151 Fragments arranged by size: 5151 821 629 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5608 809 Size: 507 4292 1802 Fragments arranged by size: 4292 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4048 4937 6566 Size: 54 1425 50 275 18 2218 889 1629 Cuts at: 6566 8 Size: 43 Fragments arranged by size: 2218 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4048 1537 Size: 275 2236 4090 Fragments arranged by size: 4090 2236 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2985 3558 3613 3628 4531 1353 Size: 1632 573 55 15 903 3423 Fragments arranged by size: 3423 1632 903 573 55 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5188 5562 5708 387 Size: 1064 138 3599 374 146 1280 Fragments arranged by size: 3599 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2651 2871 3456 3507 3722 3784 4664 Size: 393 1019 220 585 51 215 62 880 Cuts at: 4664 5261 6048 6316 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1019 880 787 597 585 393 268 220 215 62 51 EcoNI CCTnn'n_nnAGG Cuts at: 2160 2160 Size: 6601 EcoRI G'AATT_C Cuts at: 3910 4288 3910 Size: 378 6223 FspI TGC'GCA Cuts at: 2582 3584 3821 5107 2582 Size: 1002 237 1286 4076 Fragments arranged by size: 4076 1286 1002 237 HaeII r_GCGC'y Cuts at: 1678 2046 5748 1678 Size: 368 3702 2531 Fragments arranged by size: 3702 2531 368 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3656 3688 5242 5274 5316 Size: 32 135 32 2084 32 1554 32 42 Cuts at: 5316 5348 1373 Size: 32 2626 Fragments arranged by size: 2626 2084 1554 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3751 3945 4191 669 Size: 988 281 1813 194 246 3079 Fragments arranged by size: 3079 1813 988 281 246 194 HindIII A'AGCT_T Cuts at: 748 2833 748 Size: 2085 4516 HpaI GTT'AAC Cuts at: 4191 4191 Size: 6601 KpnI G_GTAC'C Cuts at: 3203 3926 3203 Size: 723 5878 MfeI C'AATT_G Cuts at: 2328 4200 2328 Size: 1872 4729 MluI A'CGCG_T Cuts at: 6477 6477 Size: 6601 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3019 3578 5715 5899 1305 Size: 1714 559 2137 184 2007 Fragments arranged by size: 2137 2007 1714 559 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4779 Fragments arranged by size: 4779 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2796 4559 4918 5077 518 Size: 648 1630 1763 359 159 2042 Fragments arranged by size: 2042 1763 1630 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2629 4017 4694 5842 6087 1606 Size: 1023 1388 677 1148 245 2120 Fragments arranged by size: 2120 1388 1148 1023 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5110 Fragments arranged by size: 5110 939 552 NdeI CA'TA_TG Cuts at: 387 2550 387 Size: 2163 4438 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6601 NotI GC'GGCC_GC Cuts at: 4048 4048 Size: 6601 NspI r_CATG'y Cuts at: 1132 1229 2301 2566 3959 5504 1132 Size: 97 1072 265 1393 1545 2229 Fragments arranged by size: 2229 1545 1393 1072 265 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6601 PciI A'CATG_T Cuts at: 5500 5500 Size: 6601 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6601 PfoI T'CCnGG_A Cuts at: 3460 3460 Size: 6601 PmeI GTTT'AAAC Cuts at: 3905 3905 Size: 6601 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4670 4702 6183 6215 4670 Size: 32 1481 32 5056 Fragments arranged by size: 5056 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6601 PsiI TTA'TAA Cuts at: 2169 4171 2169 Size: 2002 4599 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2055 2087 2850 2882 2055 Size: 32 763 32 5774 Fragments arranged by size: 5774 763 32 32 PstI C_TGCA'G Cuts at: 830 3953 830 Size: 3123 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6601 PvuII CAG'CTG Cuts at: 2629 2629 Size: 6601 SalI G'TCGA_C Cuts at: 1936 3943 1936 Size: 2007 4594 SbfI CC_TGCA'GG Cuts at: 3953 3953 Size: 6601 ScaI AGT'ACT Cuts at: 4849 6561 4849 Size: 1712 4889 SfcI C'TryA_G Cuts at: 826 1045 2160 3949 5084 5765 5956 826 Size: 219 1115 1789 1135 681 191 1471 Fragments arranged by size: 1789 1471 1135 1115 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6601 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6601 SmaI CCC'GGG Cuts at: 1872 3928 1872 Size: 2056 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2653 3179 4705 5606 5868 820 Size: 197 926 710 526 1526 901 262 1553 Fragments arranged by size: 1553 1526 926 901 710 526 262 197 SnaBI TAC'GTA Cuts at: 493 4392 493 Size: 3899 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6601 SphI G_CATG'C Cuts at: 1132 3959 1132 Size: 2827 3774 SspI AAT'ATT Cuts at: 5 52 2725 4525 5 Size: 47 2673 1800 2081 Fragments arranged by size: 2673 2081 1800 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6601 StyI C'CwwG_G Cuts at: 513 1065 2004 2870 4040 513 Size: 552 939 866 1170 3074 Fragments arranged by size: 3074 1170 939 866 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3107 4628 4645 4798 4983 5428 325 Size: 1255 1527 1521 17 153 185 445 1498 Fragments arranged by size: 1527 1521 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3113 4847 6559 Size: 274 80 33 51 1443 1136 1734 1712 Cuts at: 6559 96 Size: 138 Fragments arranged by size: 1734 1712 1443 1136 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6561 6593 6561 Size: 32 6569 Tth111I GACn'n_nGTC Cuts at: 1316 3456 1316 Size: 2140 4461 XbaI T'CTAG_A Cuts at: 2767 3347 3937 2767 Size: 580 590 5431 Fragments arranged by size: 5431 590 580 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 3250 2011 Size: 1239 5362 XhoI C'TCGA_G Cuts at: 1943 2653 1943 Size: 710 5891 XmaI C'CCGG_G Cuts at: 1870 3926 1870 Size: 2056 4545 XmnI GAAnn'nnTTC Cuts at: 3481 4730 3481 Size: 1249 5352 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4409 276 Size: 53 83 186 936 2875 2468 Fragments arranged by size: 2875 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI