(Linear) (Six-base) MAPSORT of: KIEE2697.seq Check: 7066 from: 1 to: 1896 KIEE2697.orf With 182 enzymes: SgfI * June 16, 2017 16:55 .. AccI GT'mk_AC Cuts at: 0 1280 1419 1896 Size: 1280 139 477 Fragments arranged by size: 1280 477 139 Acc65I G'GTAC_C Cuts at: 0 1194 1896 Size: 1194 702 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 866 1451 1502 1717 1896 Size: 866 585 51 215 179 Fragments arranged by size: 866 585 215 179 51 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 437 1896 Size: 437 1459 AlwNI CAG_nnn'CTG Cuts at: 0 1510 1896 Size: 1510 386 ApoI r'AATT_y Cuts at: 0 838 1005 1896 Size: 838 167 891 Fragments arranged by size: 891 838 167 AvaI C'yCGr_G Cuts at: 0 648 1896 Size: 648 1248 BamHI G'GATC_C Cuts at: 0 1726 1896 Size: 1726 170 BanI G'GyrC_C Cuts at: 0 463 1194 1896 Size: 463 731 702 Fragments arranged by size: 731 702 463 BanII G_rGCy'C Cuts at: 0 31 1896 Size: 31 1865 BbsI GAAGACnn'nnnn_ Cuts at: 0 410 1896 Size: 410 1486 BglI GCCn_nnn'nGGC Cuts at: 0 117 430 1896 Size: 117 313 1466 Fragments arranged by size: 1466 313 117 BglII A'GATC_T Cuts at: 0 337 681 1896 Size: 337 344 1215 Fragments arranged by size: 1215 344 337 Bme1580I G_kGCm'C Cuts at: 0 1442 1896 Size: 1442 454 BmgBI CAC'GTC Cuts at: 0 1636 1896 Size: 1636 260 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1543 1896 Size: 1543 353 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 646 1779 1896 Size: 646 1133 117 Fragments arranged by size: 1133 646 117 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1159 1896 Size: 1159 737 BsaAI yAC'GTr Cuts at: 0 1690 1896 Size: 1690 206 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1651 1681 1896 Size: 1651 30 215 Fragments arranged by size: 1651 215 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 19 1598 1655 1896 Size: 19 1579 57 241 Fragments arranged by size: 1579 241 57 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 762 1896 Size: 762 1134 BsiHKAI G_wGCw'C Cuts at: 0 543 1896 Size: 543 1353 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1514 1580 1896 Size: 1514 66 316 Fragments arranged by size: 1514 316 66 Bsp1286I G_dGCh'C Cuts at: 0 31 543 1442 1896 Size: 31 512 899 454 Fragments arranged by size: 899 512 454 31 BspHI T'CATG_A Cuts at: 0 69 132 657 1896 Size: 69 63 525 1239 Fragments arranged by size: 1239 525 69 63 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 366 784 1896 Size: 366 418 1112 Fragments arranged by size: 1112 418 366 BssSI C'ACGA_G Cuts at: 0 1879 1896 Size: 1879 17 BstAPI GCAn_nnn'nTGC Cuts at: 0 298 463 1492 1896 Size: 298 165 1029 404 Fragments arranged by size: 1029 404 298 165 BstYI r'GATC_y Cuts at: 0 337 681 1726 1896 Size: 337 344 1045 170 Fragments arranged by size: 1045 344 337 170 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1212 1896 Size: 1212 684 BtsI GCAGTG_nn' Cuts at: 0 749 818 1896 Size: 749 69 1078 Fragments arranged by size: 1078 749 69 DraI TTT'AAA Cuts at: 0 450 1271 1896 Size: 450 821 625 Fragments arranged by size: 821 625 450 EarI CTCTTCn'nnn_ Cuts at: 0 980 1553 1608 1623 1896 Size: 980 573 55 15 273 Fragments arranged by size: 980 573 273 55 15 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 646 866 1451 1502 1717 1779 1896 Size: 646 220 585 51 215 62 117 Fragments arranged by size: 646 585 220 215 117 62 51 EcoNI CCTnn'n_nnAGG Cuts at: 0 155 1896 Size: 155 1741 FspI TGC'GCA Cuts at: 0 577 1579 1816 1896 Size: 577 1002 237 80 Fragments arranged by size: 1002 577 237 80 HaeII r_GCGC'y Cuts at: 0 41 1896 Size: 41 1855 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1651 1683 1896 Size: 1651 32 213 Fragments arranged by size: 1651 213 32 HincII GTy'rAC Cuts at: 0 1746 1896 Size: 1746 150 HindIII A'AGCT_T Cuts at: 0 828 1896 Size: 828 1068 KpnI G_GTAC'C Cuts at: 0 1198 1896 Size: 1198 698 MfeI C'AATT_G Cuts at: 0 323 1896 Size: 323 1573 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1014 1573 1896 Size: 1014 559 323 Fragments arranged by size: 1014 559 323 MslI CAynn'nnrTG Cuts at: 0 791 1896 Size: 791 1105 MspA1I CmG'CkG Cuts at: 0 624 1896 Size: 624 1272 NdeI CA'TA_TG Cuts at: 0 545 1896 Size: 545 1351 NspI r_CATG'y Cuts at: 0 296 561 1896 Size: 296 265 1335 Fragments arranged by size: 1335 296 265 PfoI T'CCnGG_A Cuts at: 0 1455 1896 Size: 1455 441 PsiI TTA'TAA Cuts at: 0 164 1896 Size: 164 1732 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 50 82 845 877 1896 Size: 50 32 763 32 1019 Fragments arranged by size: 1019 763 50 32 32 PvuII CAG'CTG Cuts at: 0 624 1896 Size: 624 1272 SfcI C'TryA_G Cuts at: 0 155 1896 Size: 155 1741 SmlI C'TyrA_G Cuts at: 0 648 1174 1896 Size: 648 526 722 Fragments arranged by size: 722 648 526 SspI AAT'ATT Cuts at: 0 720 1896 Size: 720 1176 StyI C'CwwG_G Cuts at: 0 865 1896 Size: 865 1031 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1102 1896 Size: 1102 794 TatI w'GTAC_w Cuts at: 0 1108 1896 Size: 1108 788 Tth111I GACn'n_nGTC Cuts at: 0 1451 1896 Size: 1451 445 XbaI T'CTAG_A Cuts at: 0 762 1342 1896 Size: 762 580 554 Fragments arranged by size: 762 580 554 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1245 1896 Size: 1245 651 XhoI C'TCGA_G Cuts at: 0 648 1896 Size: 648 1248 XmnI GAAnn'nnTTC Cuts at: 0 1476 1896 Size: 1476 420 Enzymes that do cut: AccI Acc65I AcuI AlfI AlwNI ApoI AvaI BamHI BanI BanII BbsI BglI BglII Bme1580I BmgBI BplI BpmI BpuEI BsaAI BsaXI BseRI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BssSI BstAPI BstYI BtgZI BtsI DraI EarI Eco57MI EcoNI FspI HaeII Hin4I HincII HindIII KpnI MfeI MmeI MslI MspA1I NdeI NspI PfoI PsiI PsrI PvuII SfcI SmlI SspI StyI TaqII TatI Tth111I XbaI XcmI XhoI XmnI Enzymes that do not cut: AarI AatII AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI BclI BfrBI BlpI BmrI BmtI Bpu10I BsaI BsaBI BsaHI BsaWI BseYI BsiEI BsiWI BsmI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BstBI BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII DrdI EaeI EagI EciI EcoICRI EcoO109I EcoRI EcoRV FalI FseI FspAI HpaI KasI MluI MscI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI TstI XmaI ZraI