################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BanII BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrDI BsrFI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HapII HindIII HpaI HpaII KasI KpnI MamI MluI MluNI MroI MscI MspI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacI SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AcyI 46 GGCGTC G[GA]^CG[CT]C AocI 847 CCTCAGG CC^T[ACGT]AGG AseI 231 ATTAAT AT^TAAT AsnI 231 ATTAAT AT^TAAT AspHI 681 GAGCAC G[AT]GC[AT]^C BamHI 403 GGATCC G^GATCC BanI 339 GGCACC G^G[CT][GA]CC BciVI 588 GTATCC GTATCC(6/5) BfaI 562 CTAG C^TAG BmyI 681 GAGCAC G[AGT]GC[ACT]^C BsaHI 46 GGCGTC G[GA]^CG[CT]C BsaI 305 GAGACC GGTCTC(1/5) BseRI 712 CTCCTC GAGGAG(10/8) BsgI 116 GTGCAG GTGCAG(16/14) BsiEI 128 CGGCCG CG[GA][CT]^CG BsiHKAI 681 GAGCAC G[AT]GC[AT]^C BsmI 1173 GCATTC GAATGC(1/-1) Bsp1286I 681 GAGCAC G[AGT]GC[ACT]^C BspHI 311 TCATGA T^CATGA BsrBI 1228 CCGCTC CCGCTC(-3/-3) BssHII 36 GCGCGC G^CGCGC BstXI 97 CCATGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 403 GGATCC [GA]^GATC[CT] Bsu36I 847 CCTCAGG CC^T[ACGT]AGG EagI 128 CGGCCG C^GGCCG EclXI 128 CGGCCG C^GGCCG Eco52I 128 CGGCCG C^GGCCG Eco81I 847 CCTCAGG CC^T[ACGT]AGG FauI 43 GCGGG CCCGC(4/6) HaeII 859 AGCGCC [GA]GCGC^[CT] HgaI 47 GCGTC GACGC(5/10) Hin1I 46 GGCGTC G[GA]^CG[CT]C HincII 826 GTCAAC GT[CT]^[GA]AC HindII 826 GTCAAC GT[CT]^[GA]AC KspI 41 CCGCGG CCGC^GG MaeI 562 CTAG C^TAG MaeII 512 ACGT A^CGT MfeI 536 CAATTG C^AATTG MflI 403 GGATCC [GA]^GATC[CT] MslI 98 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 536 CAATTG C^AATTG NcoI 97 CCATGG C^CATGG NspI 153 GCATGC [GA]CATG^[CT] PflMI 74 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 951 GGGTCCT [GA]G^G[AT]CC[CT] PshBI 231 ATTAAT AT^TAAT RcaI 311 TCATGA T^CATGA SacII 41 CCGCGG CCGC^GG SapI 1115 GAAGAGC GCTCTTC(1/4) SphI 153 GCATGC GCATG^C SspI 228 AATATT AAT^ATT TaqI 1067 TCGA T^CGA TthHB8I 1067 TCGA T^CGA Van91I 74 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 403 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AlwNI 75 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 1279 CAGCAGCTG BpmI 81 CTGGAG CTGGAG(16/14) 889 CTGGAG BsmFI 657 GGGAC GGGAC(10/14) 468 GTCCC DsaI 41 CCGCGG C^C[GA][CT]GG 97 CCATGG EaeI 128 CGGCCG [CT]^GGCC[GA] 1160 TGGCCG MaeIII 133 GTCAC ^GT[ACGT]AC 1034 GTGAC PstI 122 CTGCAG CTGCA^G 1248 CTGCAG ScaI 893 AGTACT AGT^ACT 1070 AGTACT SfaNI 939 GATGC GCATC(5/9) 70 GCATC SfcI 122 CTGCAG C^T[GA][CT]AG 1248 CTGCAG TfiI 372 GATTC G^A[AT]TC 967 GAATC Tsp45I 133 GTCAC ^GT[GC]AC 1034 GTGAC Tsp509I 464 AATT ^AATT 537 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AvaII BbvI BsaJI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI Ksp632I MboI MboII MnlI MseI MspA1I MvaI MvnI MwoI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SmlI StyI Tru9I TseI TspRI