(Circular) (Six-base) MAPSORT of: pFN21AE2702.seq Check: 1583 from: 1 to: 5992 pFN21AE2702 5992 bp With 182 enzymes: SgfI * September 11, 2017 14:08 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3802 278 Size: 53 83 186 936 2266 2468 Fragments arranged by size: 2468 2266 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3335 1937 Size: 1398 4594 Acc65I G'GTAC_C Cuts at: 3313 3313 Size: 5992 AclI AA'CG_TT Cuts at: 1563 4119 4492 1563 Size: 2556 373 3063 Fragments arranged by size: 3063 2556 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4055 5439 5707 1632 Size: 2423 1384 268 1917 Fragments arranged by size: 2423 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5795 AflIII A'CryG_T Cuts at: 1203 4891 5868 1203 Size: 3688 977 1327 Fragments arranged by size: 3688 1327 977 AgeI A'CCGG_T Cuts at: 4823 4823 Size: 5992 AhdI GACnn_n'nnGTC Cuts at: 4721 4721 Size: 5992 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5992 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5992 AlwNI CAG_nnn'CTG Cuts at: 2085 3289 3735 5307 2085 Size: 1204 446 1572 2770 Fragments arranged by size: 2770 1572 1204 446 ApaLI G'TGCA_C Cuts at: 4049 5205 4049 Size: 1156 4836 ApoI r'AATT_y Cuts at: 1493 3301 3527 3679 5788 1493 Size: 1808 226 152 2109 1697 Fragments arranged by size: 2109 1808 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2237 4546 160 Size: 872 1205 2309 1606 Fragments arranged by size: 2309 1606 1205 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5992 AvaI C'yCGr_G Cuts at: 1870 1943 3317 1870 Size: 73 1374 4545 Fragments arranged by size: 4545 1374 73 BamHI G'GATC_C Cuts at: 2408 3322 3689 2408 Size: 914 367 4711 Fragments arranged by size: 4711 914 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2344 3313 4768 618 Size: 325 217 627 557 969 1455 1842 Fragments arranged by size: 1842 1455 969 627 557 325 217 BanII G_rGCy'C Cuts at: 1391 3311 1391 Size: 1920 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5021 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4183 4217 1903 Size: 34 50 34 2162 34 3678 Fragments arranged by size: 3678 2162 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2604 3884 5094 2604 Size: 1280 1210 3502 Fragments arranged by size: 3502 1280 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5992 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4603 136 Size: 107 122 71 631 605 2931 1525 Fragments arranged by size: 2931 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5987 5987 Size: 5992 BlpI GC'TnA_GC Cuts at: 3409 3409 Size: 5992 Bme1580I G_kGCm'C Cuts at: 1790 4053 5209 1790 Size: 2263 1156 2573 Fragments arranged by size: 2573 2263 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5992 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3723 4681 442 Size: 416 53 83 399 24 2306 958 1753 Fragments arranged by size: 2306 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5992 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2107 2915 4652 1239 Size: 868 808 1737 2579 Fragments arranged by size: 2579 1737 868 808 Bpu10I CC'TnA_GC Cuts at: 3224 3224 Size: 5992 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2163 2792 3066 4117 4982 5280 2163 Size: 629 274 1051 865 298 2875 Fragments arranged by size: 2875 1051 865 629 298 274 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2304 4655 882 Size: 617 805 2351 2219 Fragments arranged by size: 2351 2219 805 617 BsaAI yAC'GTr Cuts at: 493 1204 3783 493 Size: 711 2579 2702 Fragments arranged by size: 2702 2579 711 BsaBI GATnn'nnATC Cuts at: 5986 5986 Size: 5992 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2052 3799 4181 4816 Size: 53 83 186 936 519 1747 382 635 Cuts at: 4816 275 Size: 1451 Fragments arranged by size: 1747 1451 936 635 519 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4425 4823 5097 5244 5655 4425 Size: 398 274 147 411 4762 Fragments arranged by size: 4762 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2080 2110 2594 2624 2700 Size: 30 165 30 677 30 484 30 76 Cuts at: 2700 2730 1178 Size: 30 4440 Fragments arranged by size: 4440 677 484 165 76 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2708 1186 Size: 1522 4470 BseYI C'CCAG_C Cuts at: 2154 5195 5691 2154 Size: 3041 496 2455 Fragments arranged by size: 3041 2455 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2142 1163 Size: 573 406 5013 Fragments arranged by size: 5013 573 406 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2136 3442 4203 5231 1540 Size: 275 186 135 1306 761 1028 2301 Fragments arranged by size: 2301 1306 1028 761 275 186 135 BsiHKAI G_wGCw'C Cuts at: 2690 4053 4138 5209 2690 Size: 1363 85 1071 3473 Fragments arranged by size: 3473 1363 1071 85 BsmI GAATG_Cn' Cuts at: 3178 3503 3596 3178 Size: 325 93 5574 Fragments arranged by size: 5574 325 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2690 3311 4053 4138 5209 1391 Size: 399 900 621 742 85 1071 2174 Fragments arranged by size: 2174 1071 900 742 621 399 85 BspEI T'CCGG_A Cuts at: 5655 5655 Size: 5992 BspHI T'CATG_A Cuts at: 2316 3881 5611 2316 Size: 1565 1730 2697 Fragments arranged by size: 2697 1730 1565 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3347 844 Size: 2503 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3235 3879 1394 Size: 429 1412 644 3507 Fragments arranged by size: 3507 1412 644 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4487 4661 65 Size: 1183 3239 174 1396 Fragments arranged by size: 3239 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4636 4823 1700 Size: 110 2826 187 2869 Fragments arranged by size: 2869 2826 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5992 BssHII G'CGCG_C Cuts at: 1925 2041 1925 Size: 116 5876 BssSI C'ACGA_G Cuts at: 4052 5064 4052 Size: 1012 4980 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5992 BstBI TT'CG_AA Cuts at: 4796 4796 Size: 5992 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5992 BstXI CCAn_nnnn'nTGG Cuts at: 2109 2109 Size: 5992 BstYI r'GATC_y Cuts at: 1972 2408 3322 3689 4074 4091 5543 5629 5641 Size: 436 914 367 385 17 1452 86 12 Cuts at: 5641 5987 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 914 436 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2853 2853 Size: 5992 BtgI C'CryG_G Cuts at: 513 1065 2046 2102 513 Size: 552 981 56 4403 Fragments arranged by size: 4403 981 552 56 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2338 487 Size: 683 56 1112 4141 Fragments arranged by size: 4141 1112 683 56 BtsI GCAGTG_nn' Cuts at: 2813 3508 4302 4322 2813 Size: 695 794 20 4483 Fragments arranged by size: 4483 794 695 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5992 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3082 3117 529 Size: 35 2518 35 3404 Fragments arranged by size: 3404 2518 35 35 DraI TTT'AAA Cuts at: 3296 3296 Size: 5992 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4999 809 Size: 507 3683 1802 Fragments arranged by size: 3683 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2133 3165 3439 Size: 54 1425 50 275 18 303 1032 274 Cuts at: 3439 4328 5957 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1032 889 303 275 274 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2133 3439 1537 Size: 275 321 1306 4090 Fragments arranged by size: 4090 1306 321 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2189 2303 2541 2771 3115 3922 1353 Size: 836 114 238 230 344 807 3423 Fragments arranged by size: 3423 836 807 344 238 230 114 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2421 4579 4953 5099 387 Size: 1064 138 832 2158 374 146 1280 Fragments arranged by size: 2158 1280 1064 832 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2107 2915 4055 4652 5439 5707 1239 Size: 393 475 808 1140 597 787 268 1524 Fragments arranged by size: 1524 1140 808 787 597 475 393 268 EcoO109I rG'GnC_Cy Cuts at: 2255 2957 3160 2255 Size: 702 203 5087 Fragments arranged by size: 5087 702 203 EcoRI G'AATT_C Cuts at: 3301 3679 3301 Size: 378 5614 FspI TGC'GCA Cuts at: 4498 4498 Size: 5992 HaeII r_GCGC'y Cuts at: 1678 2868 5139 1678 Size: 1190 2271 2531 Fragments arranged by size: 2531 2271 1190 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2080 2112 2400 2432 2456 Size: 32 135 32 508 32 288 32 24 Cuts at: 2456 2488 3251 3283 4633 4665 4707 4739 1373 Size: 32 763 32 1350 32 42 32 2626 Fragments arranged by size: 2626 1350 763 508 288 135 42 32 32 32 32 32 32 32 32 24 HincII GTy'rAC Cuts at: 669 1657 1938 2833 3336 3582 669 Size: 988 281 895 503 246 3079 Fragments arranged by size: 3079 988 895 503 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5992 HpaI GTT'AAC Cuts at: 3582 3582 Size: 5992 KpnI G_GTAC'C Cuts at: 3317 3317 Size: 5992 MfeI C'AATT_G Cuts at: 2541 3591 2541 Size: 1050 4942 MluI A'CGCG_T Cuts at: 5868 5868 Size: 5992 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5106 5290 1305 Size: 3801 184 2007 Fragments arranged by size: 3801 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4170 Fragments arranged by size: 4170 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2107 3950 4309 4468 518 Size: 648 941 1843 359 159 2042 Fragments arranged by size: 2042 1843 941 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2048 2071 2085 2126 2132 2607 3217 3289 Size: 442 23 14 41 6 475 610 72 Cuts at: 3289 3408 4085 5233 5478 1606 Size: 119 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 610 475 442 245 119 72 41 23 14 6 NcoI C'CATG_G Cuts at: 513 1065 2102 513 Size: 552 1037 4403 Fragments arranged by size: 4403 1037 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5992 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5992 NotI GC'GGCC_GC Cuts at: 3439 3439 Size: 5992 NspI r_CATG'y Cuts at: 1132 1229 2162 3350 4895 1132 Size: 97 933 1188 1545 2229 Fragments arranged by size: 2229 1545 1188 933 97 PasI CC'CwG_GG Cuts at: 1348 2164 1348 Size: 816 5176 PciI A'CATG_T Cuts at: 4891 4891 Size: 5992 PflMI CCAn_nnn'nTGG Cuts at: 1108 2085 3289 1108 Size: 977 1204 3811 Fragments arranged by size: 3811 1204 977 PmeI GTTT'AAAC Cuts at: 3296 3296 Size: 5992 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4061 4093 5574 5606 4061 Size: 32 1481 32 4447 Fragments arranged by size: 4447 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2957 2957 Size: 5992 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5992 PsiI TTA'TAA Cuts at: 3562 3562 Size: 5992 PstI C_TGCA'G Cuts at: 830 2131 3257 3344 830 Size: 1301 1126 87 3478 Fragments arranged by size: 3478 1301 1126 87 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5992 PvuII CAG'CTG Cuts at: 2085 2126 2607 3217 3289 2085 Size: 41 481 610 72 4788 Fragments arranged by size: 4788 610 481 72 41 SacII CC_GC'GG Cuts at: 2049 2049 Size: 5992 SalI G'TCGA_C Cuts at: 1936 3334 1936 Size: 1398 4594 SapI GCTCTTCn'nnn_ Cuts at: 3115 3115 Size: 5992 SbfI CC_TGCA'GG Cuts at: 3344 3344 Size: 5992 ScaI AGT'ACT Cuts at: 2900 3077 4240 5952 2900 Size: 177 1163 1712 2940 Fragments arranged by size: 2940 1712 1163 177 SfcI C'TryA_G Cuts at: 826 1045 2127 3253 3340 4475 5156 5347 826 Size: 219 1082 1126 87 1135 681 191 1471 Fragments arranged by size: 1471 1135 1126 1082 681 219 191 87 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5992 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5992 SmaI CCC'GGG Cuts at: 1872 3319 1872 Size: 1447 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2178 2771 3081 4096 4997 5259 Size: 197 926 235 593 310 1015 901 262 Cuts at: 5259 820 Size: 1553 Fragments arranged by size: 1553 1015 926 901 593 310 262 235 197 SnaBI TAC'GTA Cuts at: 493 3783 493 Size: 3290 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5992 SphI G_CATG'C Cuts at: 1132 2162 3350 1132 Size: 1030 1188 3774 Fragments arranged by size: 3774 1188 1030 SspI AAT'ATT Cuts at: 5 52 2235 3916 5 Size: 47 2183 1681 2081 Fragments arranged by size: 2183 2081 1681 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5992 StyI C'CwwG_G Cuts at: 513 1065 2102 2931 3093 3431 513 Size: 552 1037 829 162 338 3074 Fragments arranged by size: 3074 1037 829 552 338 162 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2322 4019 4036 4189 4374 4819 325 Size: 1255 742 1697 17 153 185 445 1498 Fragments arranged by size: 1697 1498 1255 742 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2601 2898 3075 Size: 274 80 33 51 1443 624 297 177 Cuts at: 3075 4238 5950 96 Size: 1163 1712 138 Fragments arranged by size: 1712 1443 1163 624 297 274 177 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2104 2136 2578 2610 2700 2732 5952 5984 2104 Size: 32 442 32 90 32 3220 32 2112 Fragments arranged by size: 3220 2112 442 90 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5992 XbaI T'CTAG_A Cuts at: 3328 3328 Size: 5992 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3286 3286 Size: 5992 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5992 XmaI C'CCGG_G Cuts at: 1870 3317 1870 Size: 1447 4545 XmnI GAAnn'nnTTC Cuts at: 4121 4121 Size: 5992 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3800 276 Size: 53 83 186 936 2266 2468 Fragments arranged by size: 2468 2266 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI