(Linear) (Six-base) MAPSORT of: KIEE2702.seq Check: 5678 from: 1 to: 1287 KIEE2702.orf With 182 enzymes: SgfI * September 11, 2017 11:55 .. AlwNI CAG_nnn'CTG Cuts at: 0 80 1284 1287 Size: 80 1204 3 Fragments arranged by size: 1204 80 3 AseI AT'TA_AT Cuts at: 0 232 1287 Size: 232 1055 BamHI G'GATC_C Cuts at: 0 403 1287 Size: 403 884 BanI G'GyrC_C Cuts at: 0 339 1287 Size: 339 948 BciVI GTATCCnnnnn_n' Cuts at: 0 599 1287 Size: 599 688 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 102 910 1287 Size: 102 808 377 Fragments arranged by size: 808 377 102 Bpu10I CC'TnA_GC Cuts at: 0 1219 1287 Size: 1219 68 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 158 787 1061 1287 Size: 158 629 274 226 Fragments arranged by size: 629 274 226 158 BsaI GGTCTCn'nnnn_ Cuts at: 0 299 1287 Size: 299 988 BsaHI Gr'CG_yC Cuts at: 0 47 1287 Size: 47 1240 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 75 105 589 619 695 725 1287 Size: 75 30 484 30 76 30 562 Fragments arranged by size: 562 484 76 75 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 703 1287 Size: 703 584 BseYI C'CCAG_C Cuts at: 0 149 1287 Size: 149 1138 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 137 1287 Size: 137 1150 BsiEI CG_ry'CG Cuts at: 0 131 1287 Size: 131 1156 BsiHKAI G_wGCw'C Cuts at: 0 685 1287 Size: 685 602 BsmI GAATG_Cn' Cuts at: 0 1173 1287 Size: 1173 114 Bsp1286I G_dGCh'C Cuts at: 0 685 1287 Size: 685 602 BspHI T'CATG_A Cuts at: 0 311 1287 Size: 311 976 BsrBI CCG'CTC Cuts at: 0 1230 1287 Size: 1230 57 BssHII G'CGCG_C Cuts at: 0 36 1287 Size: 36 1251 BstXI CCAn_nnnn'nTGG Cuts at: 0 104 1287 Size: 104 1183 BstYI r'GATC_y Cuts at: 0 403 1287 Size: 403 884 Bsu36I CC'TnA_GG Cuts at: 0 848 1287 Size: 848 439 BtgI C'CryG_G Cuts at: 0 41 97 1287 Size: 41 56 1190 Fragments arranged by size: 1190 56 41 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 333 1287 Size: 333 954 BtsI GCAGTG_nn' Cuts at: 0 808 1287 Size: 808 479 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1077 1112 1287 Size: 1077 35 175 Fragments arranged by size: 1077 175 35 EaeI y'GGCC_r Cuts at: 0 128 1160 1287 Size: 128 1032 127 Fragments arranged by size: 1032 128 127 EagI C'GGCC_G Cuts at: 0 128 1287 Size: 128 1159 EarI CTCTTCn'nnn_ Cuts at: 0 184 298 536 766 1110 1287 Size: 184 114 238 230 344 177 Fragments arranged by size: 344 238 230 184 177 114 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 416 1287 Size: 416 871 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 102 910 1287 Size: 102 808 377 Fragments arranged by size: 808 377 102 EcoO109I rG'GnC_Cy Cuts at: 0 250 952 1155 1287 Size: 250 702 203 132 Fragments arranged by size: 702 250 203 132 HaeII r_GCGC'y Cuts at: 0 863 1287 Size: 863 424 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 75 107 395 427 451 483 1246 1278 Size: 75 32 288 32 24 32 763 32 Cuts at: 1278 1287 Size: 9 Fragments arranged by size: 763 288 75 32 32 32 32 24 9 HincII GTy'rAC Cuts at: 0 828 1287 Size: 828 459 MfeI C'AATT_G Cuts at: 0 536 1287 Size: 536 751 MslI CAynn'nnrTG Cuts at: 0 102 1287 Size: 102 1185 MspA1I CmG'CkG Cuts at: 0 43 66 80 121 127 602 1212 1284 Size: 43 23 14 41 6 475 610 72 Cuts at: 1284 1287 Size: 3 Fragments arranged by size: 610 475 72 43 41 23 14 6 3 NcoI C'CATG_G Cuts at: 0 97 1287 Size: 97 1190 NspI r_CATG'y Cuts at: 0 157 1287 Size: 157 1130 PasI CC'CwG_GG Cuts at: 0 159 1287 Size: 159 1128 PflMI CCAn_nnn'nTGG Cuts at: 0 80 1287 Size: 80 1207 PpuMI rG'GwC_Cy Cuts at: 0 952 1287 Size: 952 335 PstI C_TGCA'G Cuts at: 0 126 1252 1287 Size: 126 1126 35 Fragments arranged by size: 1126 126 35 PvuII CAG'CTG Cuts at: 0 80 121 602 1212 1284 1287 Size: 80 41 481 610 72 3 Fragments arranged by size: 610 481 80 72 41 3 SacII CC_GC'GG Cuts at: 0 44 1287 Size: 44 1243 SapI GCTCTTCn'nnn_ Cuts at: 0 1110 1287 Size: 1110 177 ScaI AGT'ACT Cuts at: 0 895 1072 1287 Size: 895 177 215 Fragments arranged by size: 895 215 177 SfcI C'TryA_G Cuts at: 0 122 1248 1287 Size: 122 1126 39 Fragments arranged by size: 1126 122 39 SmlI C'TyrA_G Cuts at: 0 173 766 1076 1287 Size: 173 593 310 211 Fragments arranged by size: 593 310 211 173 SphI G_CATG'C Cuts at: 0 157 1287 Size: 157 1130 SspI AAT'ATT Cuts at: 0 230 1287 Size: 230 1057 StyI C'CwwG_G Cuts at: 0 97 926 1088 1287 Size: 97 829 162 199 Fragments arranged by size: 829 199 162 97 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 317 1287 Size: 317 970 TatI w'GTAC_w Cuts at: 0 596 893 1070 1287 Size: 596 297 177 217 Fragments arranged by size: 596 297 217 177 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 99 131 573 605 695 727 1287 Size: 99 32 442 32 90 32 560 Fragments arranged by size: 560 442 99 90 32 32 32 Enzymes that do cut: AlwNI AseI BamHI BanI BciVI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BsrBI BssHII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI EaeI EagI EarI EciI Eco57MI EcoO109I HaeII Hin4I HincII MfeI MslI MspA1I NcoI NspI PasI PflMI PpuMI PstI PvuII SacII SapI ScaI SfcI SmlI SphI SspI StyI TaqII TatI TstI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AsiSI AvaI AvrII BaeI BanII BbeI BbsI BbvCI BcgI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BsaAI BsaBI BsaWI BsiWI BsmBI BspEI BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstZ17I ClaI DraI DraIII DrdI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI KpnI MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI