################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI Alw44I ApaLI AscI AseI AsnI Asp700I Asp718I AviII AvrII BalI BbvCI BcgI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BsrBI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraIII EarI EcoO65I EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccII 1279 CGCG CG^CG AflIII 524 ACATGT A^C[GA][CT]GT AhdI 1446 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1690 CCTCAGG CC^T[ACGT]AGG Aor51HI 27 AGCGCT AGC^GCT ApaI 1548 GGGCCC GGGCC^C AspEI 1446 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 1161 GGATCC G^GATCC BbeI 436 GGCGCC GGCGC^C BbrPI 774 CACGTG CAC^GTG BbsI 958 GAAGAC GAAGAC(2/6) BciVI 1736 GTATCC GTATCC(6/5) BfaI 1267 CTAG C^TAG BglI 928 GCCTGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 958 GAAGAC GAAGAC(2/6) BsiEI 17 CGGCCG CG[GA][CT]^CG BsmI 1600 GAATGC GAATGC(1/-1) BspLU11I 524 ACATGT A^CATGT BsrDI 270 CATTGC GCAATG(2/0) BstUI 1279 CGCG CG^CG BstYI 1161 GGATCC [GA]^GATC[CT] Bsu36I 1690 CCTCAGG CC^T[ACGT]AGG DraI 1033 TTTAAA TTT^AAA DrdI 739 GACTGCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 669 CCGTGG C^C[GA][CT]GG EagI 17 CGGCCG C^GGCCG Eam1105I 1446 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 17 CGGCCG C^GGCCG Eco47III 27 AGCGCT AGC^GCT Eco52I 17 CGGCCG C^GGCCG Eco81I 1690 CCTCAGG CC^T[ACGT]AGG EcoRI 293 GAATTC G^AATTC FauI 492 GCGGG CCCGC(4/6) KasI 436 GGCGCC G^GCGCC MaeI 1267 CTAG C^TAG MflI 1161 GGATCC [GA]^GATC[CT] MvnI 1279 CGCG CG^CG NaeI 1837 GCCGGC GCC^GGC NarI 436 GGCGCC GG^CGCC NgoMIV 1837 GCCGGC G^CCGGC NspI 524 ACATGT [GA]CATG^[CT] PflMI 246 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 774 CACGTG CAC^GTG PmlI 774 CACGTG CAC^GTG PpuMI 1444 GGGACCT [GA]G^G[AT]CC[CT] SfoI 436 GGCGCC GGC^GCC SmaI 728 CCCGGG CCC^GGG SmlI 1357 CTCAAG C^T[CT][GA]AG SspI 1063 AATATT AAT^ATT TaqI 529 TCGA T^CGA TfiI 1218 GATTC G^A[AT]TC TthHB8I 529 TCGA T^CGA Van91I 246 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 1161 GGATCC [GA]^GATC[CT] XmaCI 728 CCCGGG C^CCGGG XmaI 728 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AspHI 654 GTGCTC G[AT]GC[AT]^C 1527 GTGCTC BanI 436 GGCGCC G^G[CT][GA]CC 862 GGTGCC BsaAI 774 CACGTG [CT]AC^GT[GA] 819 CACGTA BsaWI 388 ACCGGA [AT]^CCGG[AT] 1450 TCCGGT BsiHKAI 654 GTGCTC G[AT]GC[AT]^C 1527 GTGCTC BsmAI 764 GAGAC GTCTC(1/5) 1570 GTCTC BsmBI 1569 CGTCTC CGTCTC(1/5) 764 GAGACG BsrFI 1116 GCCGGT [GA]^CCGG[CT] 1837 GCCGGC BssSI 99 CACGAG CACGAG(-5/-1) 915 CTCGTG BstAPI 1595 GCAGGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1664 GCATACAGTGC Cfr10I 1116 GCCGGT [GA]^CCGG[CT] 1837 GCCGGC EaeI 17 CGGCCG [CT]^GGCC[GA] 1839 CGGCCA EcoNI 1620 CCTTCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1686 CCTGCCTCAGG MslI 525 CATGTCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 867 CACCTGCGTG MspA1I 555 CAGCTG C[AC]G^C[GT]G 1491 CAGCTG PstI 310 CTGCAG CTGCA^G 710 CTGCAG PvuII 555 CAGCTG CAG^CTG 1491 CAGCTG SfaNI 376 GATGC GCATC(5/9) 482 GCATC SfcI 310 CTGCAG C^T[GA][CT]AG 710 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspI AvaI AvaII BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflFI PleI RsaI Sau3AI Sau96I ScrFI StyI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I XcmI