(Circular) (Six-base) MAPSORT of: pFN21AE2775.seq Check: 9046 from: 1 to: 6589 pFN21AE2775 6589 bp With 182 enzymes: SgfI * February 20, 2021 22:13 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2801 2882 3722 2801 Size: 81 840 5668 Fragments arranged by size: 5668 840 81 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4399 278 Size: 53 83 186 936 2863 2468 Fragments arranged by size: 2863 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3932 1937 Size: 1995 4594 Acc65I G'GTAC_C Cuts at: 3910 3910 Size: 6589 AclI AA'CG_TT Cuts at: 1563 4716 5089 1563 Size: 3153 373 3063 Fragments arranged by size: 3153 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2420 4652 6036 6304 1632 Size: 788 2232 1384 268 1917 Fragments arranged by size: 2232 1917 1384 788 268 AfeI AGC'GCT Cuts at: 2034 2034 Size: 6589 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6392 AflIII A'CryG_T Cuts at: 1203 2529 5488 6465 1203 Size: 1326 2959 977 1327 Fragments arranged by size: 2959 1327 1326 977 AgeI A'CCGG_T Cuts at: 5420 5420 Size: 6589 AhdI GACnn_n'nnGTC Cuts at: 3456 5318 3456 Size: 1862 4727 AleI CACnn'nnGTG Cuts at: 1166 2876 1166 Size: 1710 4879 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6589 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3326 3358 3326 Size: 32 6557 AlwNI CAG_nnn'CTG Cuts at: 2632 2694 3178 4332 5904 2632 Size: 62 484 1154 1572 3317 Fragments arranged by size: 3317 1572 1154 484 62 ApaI G_GGCC'C Cuts at: 3557 3557 Size: 6589 ApaLI G'TGCA_C Cuts at: 4646 5802 4646 Size: 1156 5433 ApoI r'AATT_y Cuts at: 1493 2194 2298 2347 2419 2762 3035 3406 3898 Size: 701 104 49 72 343 273 371 492 Cuts at: 3898 4124 4276 6385 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 701 492 371 343 273 226 152 104 72 49 AseI AT'TA_AT Cuts at: 160 1032 5143 160 Size: 872 4111 1606 Fragments arranged by size: 4111 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6589 AvaI C'yCGr_G Cuts at: 1870 1943 2733 2846 3539 3914 1870 Size: 73 790 113 693 375 4545 Fragments arranged by size: 4545 790 693 375 113 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3724 3757 3724 Size: 33 6556 BamHI G'GATC_C Cuts at: 3166 3919 4286 3166 Size: 753 367 5469 Fragments arranged by size: 5469 753 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2441 2867 3910 5365 618 Size: 325 217 627 654 426 1043 1455 1842 Fragments arranged by size: 1842 1455 1043 654 627 426 325 217 BanII G_rGCy'C Cuts at: 1391 2077 3557 3564 3908 1391 Size: 686 1480 7 344 4072 Fragments arranged by size: 4072 1480 686 344 7 BbeI G_GCGC'C Cuts at: 2445 2445 Size: 6589 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2970 928 Size: 971 1071 4547 Fragments arranged by size: 4547 1071 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4780 4814 1903 Size: 34 50 34 2759 34 3678 Fragments arranged by size: 3678 2759 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3752 4481 5691 3752 Size: 729 1210 4650 Fragments arranged by size: 4650 1210 729 BclI T'GATC_A Cuts at: 1548 1548 Size: 6589 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2939 5200 136 Size: 107 122 71 631 605 1267 2261 1525 Fragments arranged by size: 2261 1525 1267 631 605 122 107 71 BglII A'GATC_T Cuts at: 6584 6584 Size: 6589 BlpI GC'TnA_GC Cuts at: 4006 4006 Size: 6589 Bme1580I G_kGCm'C Cuts at: 1790 2132 2653 3557 3754 4650 5806 1790 Size: 342 521 904 197 896 1156 2573 Fragments arranged by size: 2573 1156 904 896 521 342 197 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6589 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2682 4320 5278 Size: 416 53 83 399 24 1265 1638 958 Cuts at: 5278 442 Size: 1753 Fragments arranged by size: 1753 1638 1265 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6589 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2091 2091 Size: 6589 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2099 2141 3650 5249 1239 Size: 860 42 1509 1599 2579 Fragments arranged by size: 2579 1599 1509 860 42 Bpu10I CC'TnA_GC Cuts at: 2359 2359 Size: 6589 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3347 4714 5579 5877 3347 Size: 1367 865 298 4059 Fragments arranged by size: 4059 1367 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5252 882 Size: 617 3753 2219 Fragments arranged by size: 3753 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2781 2826 4380 493 Size: 711 1577 45 1554 2702 Fragments arranged by size: 2702 1577 1554 711 45 BsaBI GATnn'nnATC Cuts at: 6583 6583 Size: 6589 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2442 2970 3840 4396 Size: 53 83 186 936 909 528 870 556 Cuts at: 4396 4778 5413 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 909 870 635 556 528 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2393 3455 5022 5420 5694 5841 6252 2393 Size: 1062 1567 398 274 147 411 2730 Fragments arranged by size: 2730 1567 1062 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3035 3065 3077 3107 3139 Size: 30 165 30 1632 30 12 30 32 Cuts at: 3139 3169 1178 Size: 30 4598 Fragments arranged by size: 4598 1632 165 32 30 30 30 30 30 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2630 3147 3150 1186 Size: 1444 517 3 4625 Fragments arranged by size: 4625 1444 517 3 BseYI C'CCAG_C Cuts at: 2601 2652 3549 5792 6288 2601 Size: 51 897 2243 496 2902 Fragments arranged by size: 2902 2243 897 496 51 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2234 2628 2707 3073 3700 3859 1163 Size: 573 498 394 79 366 627 159 3893 Fragments arranged by size: 3893 627 573 498 394 366 159 79 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2025 4039 4800 5828 1540 Size: 275 186 24 2014 761 1028 2301 Fragments arranged by size: 2301 2014 1028 761 275 186 24 BsiHKAI G_wGCw'C Cuts at: 2663 3536 4650 4735 5806 2663 Size: 873 1114 85 1071 3446 Fragments arranged by size: 3446 1114 1071 873 85 BsmI GAATG_Cn' Cuts at: 3611 4100 4193 3611 Size: 489 93 6007 Fragments arranged by size: 6007 489 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2763 3580 2763 Size: 817 5772 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2077 2132 2653 2663 3536 3557 3564 Size: 399 287 55 521 10 873 21 7 Cuts at: 3564 3754 3908 4650 4735 5806 1391 Size: 190 154 742 85 1071 2174 Fragments arranged by size: 2174 1071 873 742 521 399 287 190 154 85 55 21 10 7 BspEI T'CCGG_A Cuts at: 6252 6252 Size: 6589 BspHI T'CATG_A Cuts at: 4478 6208 4478 Size: 1730 4859 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2322 2801 2882 3722 3794 3944 844 Size: 1478 479 81 840 72 150 3489 Fragments arranged by size: 3489 1478 840 479 150 81 72 BsrBI CCG'CTC Cuts at: 1394 1823 4476 1394 Size: 429 2653 3507 Fragments arranged by size: 3507 2653 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2274 5084 5258 65 Size: 1183 1026 2810 174 1396 Fragments arranged by size: 2810 1396 1183 1026 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3121 3842 5233 5420 1700 Size: 110 1311 721 1391 187 2869 Fragments arranged by size: 2869 1391 1311 721 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6589 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6589 BssSI C'ACGA_G Cuts at: 2104 2920 4649 5661 2104 Size: 816 1729 1012 3032 Fragments arranged by size: 3032 1729 1012 816 BstAPI GCAn_nnn'nTGC Cuts at: 794 3606 3675 794 Size: 2812 69 3708 Fragments arranged by size: 3708 2812 69 BstBI TT'CG_AA Cuts at: 5393 5393 Size: 6589 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6589 BstYI r'GATC_y Cuts at: 1972 3166 3919 4286 4671 4688 6140 6226 6238 Size: 1194 753 367 385 17 1452 86 12 Cuts at: 6238 6584 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1194 753 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3696 3696 Size: 6589 BtgI C'CryG_G Cuts at: 513 1065 2674 513 Size: 552 1609 4428 Fragments arranged by size: 4428 1609 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5850 Fragments arranged by size: 5850 683 56 BtsI GCAGTG_nn' Cuts at: 2518 2663 3772 4105 4899 4919 2518 Size: 145 1109 333 794 20 4188 Fragments arranged by size: 4188 1109 794 333 145 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6589 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6554 DraI TTT'AAA Cuts at: 3040 3893 3040 Size: 853 5736 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2750 5596 809 Size: 507 1434 2846 1802 Fragments arranged by size: 2846 1802 1434 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2022 3844 4036 Size: 54 1425 50 275 18 192 1822 192 Cuts at: 4036 4925 6554 8 Size: 889 1629 43 Fragments arranged by size: 1822 1629 1425 889 275 192 192 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2022 4036 1537 Size: 275 210 2014 4090 Fragments arranged by size: 4090 2014 275 210 EarI CTCTTCn'nnn_ Cuts at: 1353 4519 1353 Size: 3166 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5176 5550 5696 387 Size: 1064 138 3587 374 146 1280 Fragments arranged by size: 3587 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2099 2141 2420 3650 4652 5249 6036 Size: 393 467 42 279 1230 1002 597 787 Cuts at: 6036 6304 1239 Size: 268 1524 Fragments arranged by size: 1524 1230 1002 787 597 467 393 279 268 42 EcoNI CCTnn'n_nnAGG Cuts at: 3629 3695 3629 Size: 66 6523 EcoO109I rG'GnC_Cy Cuts at: 2729 2996 3450 3553 2729 Size: 267 454 103 5765 Fragments arranged by size: 5765 454 267 103 EcoRI G'AATT_C Cuts at: 2298 3898 4276 2298 Size: 1600 378 4611 Fragments arranged by size: 4611 1600 378 FspI TGC'GCA Cuts at: 5095 5095 Size: 6589 HaeII r_GCGC'y Cuts at: 1678 2036 2045 2445 2671 5736 1678 Size: 358 9 400 226 3065 2531 Fragments arranged by size: 3065 2531 400 358 226 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2903 2935 3035 3067 3442 Size: 32 135 32 1331 32 100 32 375 Cuts at: 3442 3474 3560 3592 5230 5262 5304 5336 1373 Size: 32 86 32 1638 32 42 32 2626 Fragments arranged by size: 2626 1638 1331 375 135 100 86 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3933 4179 669 Size: 988 281 1995 246 3079 Fragments arranged by size: 3079 1995 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6589 HpaI GTT'AAC Cuts at: 4179 4179 Size: 6589 KasI G'GCGC_C Cuts at: 2441 2441 Size: 6589 KpnI G_GTAC'C Cuts at: 3914 3914 Size: 6589 MfeI C'AATT_G Cuts at: 4188 4188 Size: 6589 MluI A'CGCG_T Cuts at: 6465 6465 Size: 6589 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5703 5887 1305 Size: 4398 184 2007 Fragments arranged by size: 4398 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4767 Fragments arranged by size: 4767 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2534 2876 4547 4906 5065 518 Size: 648 1368 342 1671 359 159 2042 Fragments arranged by size: 2042 1671 1368 648 359 342 159 MspA1I CmG'CkG Cuts at: 1606 2562 3498 4005 4682 5830 6075 1606 Size: 956 936 507 677 1148 245 2120 Fragments arranged by size: 2120 1148 956 936 677 507 245 NaeI GCC'GGC Cuts at: 3844 3844 Size: 6589 NarI GG'CG_CC Cuts at: 2442 2442 Size: 6589 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6037 NdeI CA'TA_TG Cuts at: 387 387 Size: 6589 NgoMIV G'CCGG_C Cuts at: 3842 3842 Size: 6589 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6589 NotI GC'GGCC_GC Cuts at: 4036 4036 Size: 6589 NspI r_CATG'y Cuts at: 1132 1229 2533 3947 5492 1132 Size: 97 1304 1414 1545 2229 Fragments arranged by size: 2229 1545 1414 1304 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6589 PciI A'CATG_T Cuts at: 2529 5488 2529 Size: 2959 3630 PflMI CCAn_nnn'nTGG Cuts at: 1108 2257 1108 Size: 1149 5440 PfoI T'CCnGG_A Cuts at: 2015 2250 2015 Size: 235 6354 PmeI GTTT'AAAC Cuts at: 3893 3893 Size: 6589 PmlI CAC'GTG Cuts at: 2781 2781 Size: 6589 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4658 4690 6171 6203 4658 Size: 32 1481 32 5044 Fragments arranged by size: 5044 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3450 3450 Size: 6589 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6589 PsiI TTA'TAA Cuts at: 3476 4159 3476 Size: 683 5906 PspOMI G'GGCC_C Cuts at: 3553 3553 Size: 6589 PstI C_TGCA'G Cuts at: 830 2319 2719 3941 830 Size: 1489 400 1222 3478 Fragments arranged by size: 3478 1489 1222 400 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6589 PvuII CAG'CTG Cuts at: 2562 3498 2562 Size: 936 5653 SalI G'TCGA_C Cuts at: 1936 3931 1936 Size: 1995 4594 SbfI CC_TGCA'GG Cuts at: 3941 3941 Size: 6589 ScaI AGT'ACT Cuts at: 4837 6549 4837 Size: 1712 4877 SfcI C'TryA_G Cuts at: 826 1045 2315 2715 3937 5072 5753 5944 826 Size: 219 1270 400 1222 1135 681 191 1471 Fragments arranged by size: 1471 1270 1222 1135 681 400 219 191 SfoI GGC'GCC Cuts at: 2443 2443 Size: 6589 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6589 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6589 SmaI CCC'GGG Cuts at: 1872 2735 3916 1872 Size: 863 1181 4545 Fragments arranged by size: 4545 1181 863 SmlI C'TyrA_G Cuts at: 820 1017 1943 3362 4693 5594 5856 820 Size: 197 926 1419 1331 901 262 1553 Fragments arranged by size: 1553 1419 1331 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4380 493 Size: 3887 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6589 SphI G_CATG'C Cuts at: 1132 3947 1132 Size: 2815 3774 SspI AAT'ATT Cuts at: 5 52 3070 4513 5 Size: 47 3018 1443 2081 Fragments arranged by size: 3018 2081 1443 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6589 StyI C'CwwG_G Cuts at: 513 1065 2098 2188 3345 3564 3641 4028 513 Size: 552 1033 90 1157 219 77 387 3074 Fragments arranged by size: 3074 1157 1033 552 387 219 90 77 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3696 4616 4633 4786 4971 5416 325 Size: 1255 2116 920 17 153 185 445 1498 Fragments arranged by size: 2116 1498 1255 920 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2337 4835 6547 Size: 274 80 33 51 1443 360 2498 1712 Cuts at: 6547 96 Size: 138 Fragments arranged by size: 2498 1712 1443 360 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3139 3171 6549 6581 3139 Size: 32 3378 32 3147 Fragments arranged by size: 3378 3147 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2504 2969 3572 1316 Size: 1188 465 603 4333 Fragments arranged by size: 4333 1188 603 465 XbaI T'CTAG_A Cuts at: 3925 3925 Size: 6589 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2254 2258 2830 2254 Size: 4 572 6013 Fragments arranged by size: 6013 572 4 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6589 XmaI C'CCGG_G Cuts at: 1870 2733 3914 1870 Size: 863 1181 4545 Fragments arranged by size: 4545 1181 863 XmnI GAAnn'nnTTC Cuts at: 4718 4718 Size: 6589 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4397 276 Size: 53 83 186 936 2863 2468 Fragments arranged by size: 2863 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BbvCI BfrBI BsiWI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI