Restriction map for
pFN21AE2775
(Linear) (Six-base) MAPSORT of: KIEE2775.seq Check: 4889 from: 1 to: 1884
KIEE2775.orf
With 182 enzymes: SgfI *
February 19, 2021 20:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 796 877 1717 1884
Size: 796 81 840 167
Fragments arranged by size:
840 796 167 81
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 1884
Size: 415 1469
AfeI AGC'GCT
Cuts at: 0 29 1884
Size: 29 1855
AflIII A'CryG_T
Cuts at: 0 524 1884
Size: 524 1360
AhdI GACnn_n'nnGTC
Cuts at: 0 1451 1884
Size: 1451 433
AleI CACnn'nnGTG
Cuts at: 0 871 1884
Size: 871 1013
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1321 1353 1884
Size: 1321 32 531
Fragments arranged by size:
1321 531 32
AlwNI CAG_nnn'CTG
Cuts at: 0 627 689 1173 1884
Size: 627 62 484 711
Fragments arranged by size:
711 627 484 62
ApaI G_GGCC'C
Cuts at: 0 1552 1884
Size: 1552 332
ApoI r'AATT_y
Cuts at: 0 189 293 342 414 757 1030 1401 1884
Size: 189 104 49 72 343 273 371 483
Fragments arranged by size:
483 371 343 273 189 104 72 49
AvaI C'yCGr_G
Cuts at: 0 728 841 1534 1884
Size: 728 113 693 350
Fragments arranged by size:
728 693 350 113
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1719 1752 1884
Size: 1719 33 132
Fragments arranged by size:
1719 132 33
BamHI G'GATC_C
Cuts at: 0 1161 1884
Size: 1161 723
BanI G'GyrC_C
Cuts at: 0 436 862 1884
Size: 436 426 1022
Fragments arranged by size:
1022 436 426
BanII G_rGCy'C
Cuts at: 0 72 1552 1559 1884
Size: 72 1480 7 325
Fragments arranged by size:
1480 325 72 7
BbeI G_GCGC'C
Cuts at: 0 440 1884
Size: 440 1444
BbsI GAAGACnn'nnnn_
Cuts at: 0 965 1884
Size: 965 919
BciVI GTATCCnnnnn_n'
Cuts at: 0 1747 1884
Size: 1747 137
BglI GCCn_nnn'nGGC
Cuts at: 0 934 1884
Size: 934 950
Bme1580I G_kGCm'C
Cuts at: 0 127 648 1552 1749 1884
Size: 127 521 904 197 135
Fragments arranged by size:
904 521 197 135 127
BmrI ACTGGGnnnn_n'
Cuts at: 0 677 1884
Size: 677 1207
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 86 1884
Size: 86 1798
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 136 1645 1884
Size: 94 42 1509 239
Fragments arranged by size:
1509 239 94 42
Bpu10I CC'TnA_GC
Cuts at: 0 354 1884
Size: 354 1530
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1342 1884
Size: 1342 542
BsaAI yAC'GTr
Cuts at: 0 776 821 1884
Size: 776 45 1063
Fragments arranged by size:
1063 776 45
BsaHI Gr'CG_yC
Cuts at: 0 437 965 1835 1884
Size: 437 528 870 49
Fragments arranged by size:
870 528 437 49
BsaWI w'CCGG_w
Cuts at: 0 388 1450 1884
Size: 388 1062 434
Fragments arranged by size:
1062 434 388
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1030 1060 1072 1102 1134 1164 1884
Size: 1030 30 12 30 32 30 720
Fragments arranged by size:
1030 720 32 30 30 30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 625 1142 1145 1884
Size: 625 517 3 739
Fragments arranged by size:
739 625 517 3
BseYI C'CCAG_C
Cuts at: 0 596 647 1544 1884
Size: 596 51 897 340
Fragments arranged by size:
897 596 340 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 623 702 1068 1695 1854 1884
Size: 229 394 79 366 627 159 30
Fragments arranged by size:
627 394 366 229 159 79 30
BsiEI CG_ry'CG
Cuts at: 0 20 1884
Size: 20 1864
BsiHKAI G_wGCw'C
Cuts at: 0 658 1531 1884
Size: 658 873 353
Fragments arranged by size:
873 658 353
BsmI GAATG_Cn'
Cuts at: 0 1606 1884
Size: 1606 278
BsmBI CGTCTCn'nnnn_
Cuts at: 0 758 1575 1884
Size: 758 817 309
Fragments arranged by size:
817 758 309
Bsp1286I G_dGCh'C
Cuts at: 0 72 127 648 658 1531 1552 1559 1749
Size: 72 55 521 10 873 21 7 190
Cuts at: 1749 1884
Size: 135
Fragments arranged by size:
873 521 190 135 72 55 21 10
7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 317 796 877 1717 1789 1884
Size: 317 479 81 840 72 95
Fragments arranged by size:
840 479 317 95 81 72
BsrDI GCAATG_nn'
Cuts at: 0 269 1884
Size: 269 1615
BsrFI r'CCGG_y
Cuts at: 0 1116 1837 1884
Size: 1116 721 47
Fragments arranged by size:
1116 721 47
BssSI C'ACGA_G
Cuts at: 0 99 915 1884
Size: 99 816 969
Fragments arranged by size:
969 816 99
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1601 1670 1884
Size: 1601 69 214
Fragments arranged by size:
1601 214 69
BstYI r'GATC_y
Cuts at: 0 1161 1884
Size: 1161 723
Bsu36I CC'TnA_GG
Cuts at: 0 1691 1884
Size: 1691 193
BtgI C'CryG_G
Cuts at: 0 669 1884
Size: 669 1215
BtsI GCAGTG_nn'
Cuts at: 0 513 658 1767 1884
Size: 513 145 1109 117
Fragments arranged by size:
1109 513 145 117
DraI TTT'AAA
Cuts at: 0 1035 1884
Size: 1035 849
DrdI GACnn_nn'nnGTC
Cuts at: 0 745 1884
Size: 745 1139
EaeI y'GGCC_r
Cuts at: 0 17 1839 1884
Size: 17 1822 45
Fragments arranged by size:
1822 45 17
EagI C'GGCC_G
Cuts at: 0 17 1884
Size: 17 1867
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 136 415 1645 1884
Size: 94 42 279 1230 239
Fragments arranged by size:
1230 279 239 94 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1624 1690 1884
Size: 1624 66 194
Fragments arranged by size:
1624 194 66
EcoO109I rG'GnC_Cy
Cuts at: 0 724 991 1445 1548 1884
Size: 724 267 454 103 336
Fragments arranged by size:
724 454 336 267 103
EcoRI G'AATT_C
Cuts at: 0 293 1884
Size: 293 1591
HaeII r_GCGC'y
Cuts at: 0 31 40 440 666 1884
Size: 31 9 400 226 1218
Fragments arranged by size:
1218 400 226 31 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 898 930 1030 1062 1437 1469 1555 1587
Size: 898 32 100 32 375 32 86 32
Cuts at: 1587 1884
Size: 297
Fragments arranged by size:
898 375 297 100 86 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 436 1884
Size: 436 1448
MslI CAynn'nnrTG
Cuts at: 0 529 871 1884
Size: 529 342 1013
Fragments arranged by size:
1013 529 342
MspA1I CmG'CkG
Cuts at: 0 557 1493 1884
Size: 557 936 391
Fragments arranged by size:
936 557 391
NaeI GCC'GGC
Cuts at: 0 1839 1884
Size: 1839 45
NarI GG'CG_CC
Cuts at: 0 437 1884
Size: 437 1447
NgoMIV G'CCGG_C
Cuts at: 0 1837 1884
Size: 1837 47
NspI r_CATG'y
Cuts at: 0 528 1884
Size: 528 1356
PciI A'CATG_T
Cuts at: 0 524 1884
Size: 524 1360
PflMI CCAn_nnn'nTGG
Cuts at: 0 252 1884
Size: 252 1632
PfoI T'CCnGG_A
Cuts at: 0 10 245 1884
Size: 10 235 1639
Fragments arranged by size:
1639 235 10
PmlI CAC'GTG
Cuts at: 0 776 1884
Size: 776 1108
PpuMI rG'GwC_Cy
Cuts at: 0 1445 1884
Size: 1445 439
PsiI TTA'TAA
Cuts at: 0 1471 1884
Size: 1471 413
PspOMI G'GGCC_C
Cuts at: 0 1548 1884
Size: 1548 336
PstI C_TGCA'G
Cuts at: 0 314 714 1884
Size: 314 400 1170
Fragments arranged by size:
1170 400 314
PvuII CAG'CTG
Cuts at: 0 557 1493 1884
Size: 557 936 391
Fragments arranged by size:
936 557 391
SfcI C'TryA_G
Cuts at: 0 310 710 1884
Size: 310 400 1174
Fragments arranged by size:
1174 400 310
SfoI GGC'GCC
Cuts at: 0 438 1884
Size: 438 1446
SmaI CCC'GGG
Cuts at: 0 730 1884
Size: 730 1154
SmlI C'TyrA_G
Cuts at: 0 1357 1884
Size: 1357 527
SspI AAT'ATT
Cuts at: 0 1065 1884
Size: 1065 819
StyI C'CwwG_G
Cuts at: 0 93 183 1340 1559 1636 1884
Size: 93 90 1157 219 77 248
Fragments arranged by size:
1157 248 219 93 90 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1691 1884
Size: 1691 193
TatI w'GTAC_w
Cuts at: 0 332 1884
Size: 332 1552
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1134 1166 1884
Size: 1134 32 718
Fragments arranged by size:
1134 718 32
Tth111I GACn'n_nGTC
Cuts at: 0 499 964 1567 1884
Size: 499 465 603 317
Fragments arranged by size:
603 499 465 317
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 249 253 825 1884
Size: 249 4 572 1059
Fragments arranged by size:
1059 572 249 4
XmaI C'CCGG_G
Cuts at: 0 728 1884
Size: 728 1156
Enzymes that do cut:
AarI AcuI AfeI AflIII AhdI AleI AloI AlwNI
ApaI ApoI AvaI BaeI BamHI BanI BanII BbeI
BbsI BciVI BglI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BsrFI
BssSI BstAPI BstYI Bsu36I BtgI BtsI DraI DrdI
EaeI EagI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I
KasI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PflMI PfoI PmlI PpuMI PsiI PspOMI PstI PvuII
SfcI SfoI SmaI SmlI SspI StyI TaqII TatI
TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AlfI ApaLI
AscI AseI AsiSI AvrII BbvCI BcgI BclI BfrBI
BglII BlpI BmgBI BmtI BsaI BsaBI BsiWI BspEI
BspHI BsrBI BsrGI BssHII BstBI BstEII BstXI BstZ17I
BtgZI ClaI CspCI DraIII EarI EciI EcoICRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PmeI PpiI PshAI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI StuI SwaI XbaI XhoI XmnI ZraI