(Circular) (Six-base) MAPSORT of: pFN21AE2774.seq Check: 9218 from: 1 to: 6820 pFN21AE2774 6820 bp With 182 enzymes: SgfI * February 20, 2021 22:13 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2801 2882 3722 2801 Size: 81 840 5899 Fragments arranged by size: 5899 840 81 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4630 278 Size: 53 83 186 936 3094 2468 Fragments arranged by size: 3094 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4163 1937 Size: 2226 4594 Acc65I G'GTAC_C Cuts at: 4141 4141 Size: 6820 AclI AA'CG_TT Cuts at: 1563 4947 5320 1563 Size: 3384 373 3063 Fragments arranged by size: 3384 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2420 4883 6267 6535 1632 Size: 788 2463 1384 268 1917 Fragments arranged by size: 2463 1917 1384 788 268 AfeI AGC'GCT Cuts at: 2034 2034 Size: 6820 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6623 AflIII A'CryG_T Cuts at: 1203 2529 5719 6696 1203 Size: 1326 3190 977 1327 Fragments arranged by size: 3190 1327 1326 977 AgeI A'CCGG_T Cuts at: 5651 5651 Size: 6820 AhdI GACnn_n'nnGTC Cuts at: 3456 5549 3456 Size: 2093 4727 AleI CACnn'nnGTG Cuts at: 1166 2876 1166 Size: 1710 5110 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6820 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3326 3358 3326 Size: 32 6788 AlwNI CAG_nnn'CTG Cuts at: 2632 2694 3178 4563 6135 2632 Size: 62 484 1385 1572 3317 Fragments arranged by size: 3317 1572 1385 484 62 ApaI G_GGCC'C Cuts at: 3557 3557 Size: 6820 ApaLI G'TGCA_C Cuts at: 4877 6033 4877 Size: 1156 5664 ApoI r'AATT_y Cuts at: 1493 2194 2298 2347 2419 2762 3035 3406 4129 Size: 701 104 49 72 343 273 371 723 Cuts at: 4129 4355 4507 6616 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 723 701 371 343 273 226 152 104 72 49 AseI AT'TA_AT Cuts at: 160 1032 5374 160 Size: 872 4342 1606 Fragments arranged by size: 4342 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6820 AvaI C'yCGr_G Cuts at: 1870 1943 2733 2846 3539 4145 1870 Size: 73 790 113 693 606 4545 Fragments arranged by size: 4545 790 693 606 113 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3724 3757 3724 Size: 33 6787 BamHI G'GATC_C Cuts at: 3166 4014 4150 4517 3166 Size: 848 136 367 5469 Fragments arranged by size: 5469 848 367 136 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2441 2867 4141 5596 618 Size: 325 217 627 654 426 1274 1455 1842 Fragments arranged by size: 1842 1455 1274 654 627 426 325 217 BanII G_rGCy'C Cuts at: 1391 2077 3557 3564 3986 4139 1391 Size: 686 1480 7 422 153 4072 Fragments arranged by size: 4072 1480 686 422 153 7 BbeI G_GCGC'C Cuts at: 2445 2445 Size: 6820 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2970 928 Size: 971 1071 4778 Fragments arranged by size: 4778 1071 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5011 5045 1903 Size: 34 50 34 2990 34 3678 Fragments arranged by size: 3678 2990 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3752 4712 5922 3752 Size: 960 1210 4650 Fragments arranged by size: 4650 1210 960 BclI T'GATC_A Cuts at: 1548 4095 1548 Size: 2547 4273 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2939 5431 136 Size: 107 122 71 631 605 1267 2492 1525 Fragments arranged by size: 2492 1525 1267 631 605 122 107 71 BglII A'GATC_T Cuts at: 6815 6815 Size: 6820 BlpI GC'TnA_GC Cuts at: 4237 4237 Size: 6820 Bme1580I G_kGCm'C Cuts at: 1790 2132 2653 3557 3754 4881 6037 1790 Size: 342 521 904 197 1127 1156 2573 Fragments arranged by size: 2573 1156 1127 904 521 342 197 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6820 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2682 4551 5509 Size: 416 53 83 399 24 1265 1869 958 Cuts at: 5509 442 Size: 1753 Fragments arranged by size: 1869 1753 1265 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6820 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2091 2091 Size: 6820 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2099 2141 3650 5480 1239 Size: 860 42 1509 1830 2579 Fragments arranged by size: 2579 1830 1509 860 42 Bpu10I CC'TnA_GC Cuts at: 2359 3993 4006 2359 Size: 1634 13 5173 Fragments arranged by size: 5173 1634 13 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3347 4945 5810 6108 3347 Size: 1598 865 298 4059 Fragments arranged by size: 4059 1598 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5483 882 Size: 617 3984 2219 Fragments arranged by size: 3984 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2781 2826 4611 493 Size: 711 1577 45 1785 2702 Fragments arranged by size: 2702 1785 1577 711 45 BsaBI GATnn'nnATC Cuts at: 6814 6814 Size: 6820 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2442 2970 3840 4627 Size: 53 83 186 936 909 528 870 787 Cuts at: 4627 5009 5644 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 909 870 787 635 528 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2393 3455 5253 5651 5925 6072 6483 2393 Size: 1062 1798 398 274 147 411 2730 Fragments arranged by size: 2730 1798 1062 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3035 3065 3077 3107 3139 Size: 30 165 30 1632 30 12 30 32 Cuts at: 3139 3169 1178 Size: 30 4829 Fragments arranged by size: 4829 1632 165 32 30 30 30 30 30 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2630 3147 3150 4099 1186 Size: 1444 517 3 949 3907 Fragments arranged by size: 3907 1444 949 517 3 BseYI C'CCAG_C Cuts at: 2601 2652 3549 3985 4045 6023 6519 2601 Size: 51 897 436 60 1978 496 2902 Fragments arranged by size: 2902 1978 897 496 436 60 51 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2234 2628 2707 3073 3700 3859 1163 Size: 573 498 394 79 366 627 159 4124 Fragments arranged by size: 4124 627 573 498 394 366 159 79 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2025 4270 5031 6059 1540 Size: 275 186 24 2245 761 1028 2301 Fragments arranged by size: 2301 2245 1028 761 275 186 24 BsiHKAI G_wGCw'C Cuts at: 2663 3536 4881 4966 6037 2663 Size: 873 1345 85 1071 3446 Fragments arranged by size: 3446 1345 1071 873 85 BsmI GAATG_Cn' Cuts at: 3611 4331 4424 3611 Size: 720 93 6007 Fragments arranged by size: 6007 720 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2763 3580 2763 Size: 817 6003 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2077 2132 2653 2663 3536 3557 3564 Size: 399 287 55 521 10 873 21 7 Cuts at: 3564 3754 3986 4139 4881 4966 6037 1391 Size: 190 232 153 742 85 1071 2174 Fragments arranged by size: 2174 1071 873 742 521 399 287 232 190 153 85 55 21 10 7 BspEI T'CCGG_A Cuts at: 6483 6483 Size: 6820 BspHI T'CATG_A Cuts at: 4709 6439 4709 Size: 1730 5090 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2322 2801 2882 3722 3794 4175 844 Size: 1478 479 81 840 72 381 3489 Fragments arranged by size: 3489 1478 840 479 381 81 72 BsrBI CCG'CTC Cuts at: 1394 1823 3978 4707 1394 Size: 429 2155 729 3507 Fragments arranged by size: 3507 2155 729 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2274 5315 5489 65 Size: 1183 1026 3041 174 1396 Fragments arranged by size: 3041 1396 1183 1026 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3121 3842 5464 5651 1700 Size: 110 1311 721 1622 187 2869 Fragments arranged by size: 2869 1622 1311 721 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6820 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6820 BssSI C'ACGA_G Cuts at: 2104 2920 4880 5892 2104 Size: 816 1960 1012 3032 Fragments arranged by size: 3032 1960 1012 816 BstAPI GCAn_nnn'nTGC Cuts at: 794 3606 3675 794 Size: 2812 69 3939 Fragments arranged by size: 3939 2812 69 BstBI TT'CG_AA Cuts at: 5624 5624 Size: 6820 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6820 BstYI r'GATC_y Cuts at: 1972 3166 4014 4150 4517 4902 4919 6371 6457 Size: 1194 848 136 367 385 17 1452 86 Cuts at: 6457 6469 6815 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1194 848 385 367 346 136 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3696 3696 Size: 6820 BtgI C'CryG_G Cuts at: 513 1065 2674 513 Size: 552 1609 4659 Fragments arranged by size: 4659 1609 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6081 Fragments arranged by size: 6081 683 56 BtsI GCAGTG_nn' Cuts at: 2518 2663 3772 4336 5130 5150 2518 Size: 145 1109 564 794 20 4188 Fragments arranged by size: 4188 1109 794 564 145 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6820 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6785 DraI TTT'AAA Cuts at: 3040 3928 4124 3040 Size: 888 196 5736 Fragments arranged by size: 5736 888 196 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2750 5827 809 Size: 507 1434 3077 1802 Fragments arranged by size: 3077 1802 1434 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2022 3844 4267 Size: 54 1425 50 275 18 192 1822 423 Cuts at: 4267 5156 6785 8 Size: 889 1629 43 Fragments arranged by size: 1822 1629 1425 889 423 275 192 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2022 4267 1537 Size: 275 210 2245 4090 Fragments arranged by size: 4090 2245 275 210 EarI CTCTTCn'nnn_ Cuts at: 1353 4029 4750 1353 Size: 2676 721 3423 Fragments arranged by size: 3423 2676 721 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5407 5781 5927 387 Size: 1064 138 3818 374 146 1280 Fragments arranged by size: 3818 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2099 2141 2420 3650 4883 5480 6267 Size: 393 467 42 279 1230 1233 597 787 Cuts at: 6267 6535 1239 Size: 268 1524 Fragments arranged by size: 1524 1233 1230 787 597 467 393 279 268 42 EcoNI CCTnn'n_nnAGG Cuts at: 3629 3695 3629 Size: 66 6754 EcoO109I rG'GnC_Cy Cuts at: 2729 2996 3450 3553 2729 Size: 267 454 103 5996 Fragments arranged by size: 5996 454 267 103 EcoRI G'AATT_C Cuts at: 2298 4129 4507 2298 Size: 1831 378 4611 Fragments arranged by size: 4611 1831 378 FspI TGC'GCA Cuts at: 5326 5326 Size: 6820 HaeII r_GCGC'y Cuts at: 1678 2036 2045 2445 2671 5967 1678 Size: 358 9 400 226 3296 2531 Fragments arranged by size: 3296 2531 400 358 226 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2903 2935 3035 3067 3442 Size: 32 135 32 1331 32 100 32 375 Cuts at: 3442 3474 3560 3592 4006 4038 5461 5493 5535 Size: 32 86 32 414 32 1423 32 42 Cuts at: 5535 5567 1373 Size: 32 2626 Fragments arranged by size: 2626 1423 1331 414 375 135 100 86 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4164 4410 669 Size: 988 281 2226 246 3079 Fragments arranged by size: 3079 2226 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6820 HpaI GTT'AAC Cuts at: 4410 4410 Size: 6820 KasI G'GCGC_C Cuts at: 2441 2441 Size: 6820 KpnI G_GTAC'C Cuts at: 4145 4145 Size: 6820 MfeI C'AATT_G Cuts at: 4419 4419 Size: 6820 MluI A'CGCG_T Cuts at: 6696 6696 Size: 6820 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5934 6118 1305 Size: 4629 184 2007 Fragments arranged by size: 4629 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4998 Fragments arranged by size: 4998 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2534 2876 4071 4778 5137 5296 518 Size: 648 1368 342 1195 707 359 159 2042 Fragments arranged by size: 2042 1368 1195 707 648 359 342 159 MspA1I CmG'CkG Cuts at: 1606 2562 3498 3989 4045 4236 4913 6061 6306 Size: 956 936 491 56 191 677 1148 245 Cuts at: 6306 1606 Size: 2120 Fragments arranged by size: 2120 1148 956 936 677 491 245 191 56 NaeI GCC'GGC Cuts at: 3844 3844 Size: 6820 NarI GG'CG_CC Cuts at: 2442 2442 Size: 6820 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6268 NdeI CA'TA_TG Cuts at: 387 387 Size: 6820 NgoMIV G'CCGG_C Cuts at: 3842 3842 Size: 6820 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6820 NotI GC'GGCC_GC Cuts at: 4267 4267 Size: 6820 NspI r_CATG'y Cuts at: 1132 1229 2533 4178 5723 1132 Size: 97 1304 1645 1545 2229 Fragments arranged by size: 2229 1645 1545 1304 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6820 PciI A'CATG_T Cuts at: 2529 5719 2529 Size: 3190 3630 PflMI CCAn_nnn'nTGG Cuts at: 1108 2257 1108 Size: 1149 5671 PfoI T'CCnGG_A Cuts at: 2015 2250 2015 Size: 235 6585 PmeI GTTT'AAAC Cuts at: 4124 4124 Size: 6820 PmlI CAC'GTG Cuts at: 2781 2781 Size: 6820 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4889 4921 6402 6434 4889 Size: 32 1481 32 5275 Fragments arranged by size: 5275 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3450 3450 Size: 6820 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6820 PsiI TTA'TAA Cuts at: 3476 4390 3476 Size: 914 5906 PspOMI G'GGCC_C Cuts at: 3553 3553 Size: 6820 PstI C_TGCA'G Cuts at: 830 2319 2719 4172 830 Size: 1489 400 1453 3478 Fragments arranged by size: 3478 1489 1453 400 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6820 PvuII CAG'CTG Cuts at: 2562 3498 3989 4045 2562 Size: 936 491 56 5337 Fragments arranged by size: 5337 936 491 56 SalI G'TCGA_C Cuts at: 1936 4162 1936 Size: 2226 4594 SbfI CC_TGCA'GG Cuts at: 4172 4172 Size: 6820 ScaI AGT'ACT Cuts at: 5068 6780 5068 Size: 1712 5108 SfcI C'TryA_G Cuts at: 826 1045 2315 2715 4168 5303 5984 6175 826 Size: 219 1270 400 1453 1135 681 191 1471 Fragments arranged by size: 1471 1453 1270 1135 681 400 219 191 SfoI GGC'GCC Cuts at: 2443 2443 Size: 6820 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6820 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6820 SmaI CCC'GGG Cuts at: 1872 2735 4147 1872 Size: 863 1412 4545 Fragments arranged by size: 4545 1412 863 SmlI C'TyrA_G Cuts at: 820 1017 1943 3362 4924 5825 6087 820 Size: 197 926 1419 1562 901 262 1553 Fragments arranged by size: 1562 1553 1419 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4611 493 Size: 4118 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6820 SphI G_CATG'C Cuts at: 1132 4178 1132 Size: 3046 3774 SspI AAT'ATT Cuts at: 5 52 3070 4744 5 Size: 47 3018 1674 2081 Fragments arranged by size: 3018 2081 1674 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6820 StyI C'CwwG_G Cuts at: 513 1065 2098 2188 3345 3564 3641 4259 513 Size: 552 1033 90 1157 219 77 618 3074 Fragments arranged by size: 3074 1157 1033 618 552 219 90 77 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3696 4847 4864 5017 5202 5647 325 Size: 1255 2116 1151 17 153 185 445 1498 Fragments arranged by size: 2116 1498 1255 1151 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2337 5066 6778 Size: 274 80 33 51 1443 360 2729 1712 Cuts at: 6778 96 Size: 138 Fragments arranged by size: 2729 1712 1443 360 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3139 3171 6780 6812 3139 Size: 32 3609 32 3147 Fragments arranged by size: 3609 3147 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2504 2969 3572 1316 Size: 1188 465 603 4564 Fragments arranged by size: 4564 1188 603 465 XbaI T'CTAG_A Cuts at: 4156 4156 Size: 6820 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2254 2258 2830 2254 Size: 4 572 6244 Fragments arranged by size: 6244 572 4 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6820 XmaI C'CCGG_G Cuts at: 1870 2733 4145 1870 Size: 863 1412 4545 Fragments arranged by size: 4545 1412 863 XmnI GAAnn'nnTTC Cuts at: 4949 4949 Size: 6820 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4628 276 Size: 53 83 186 936 3094 2468 Fragments arranged by size: 3094 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BbvCI BfrBI BsiWI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI