################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AgeI AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BsrBI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraIII Eam1105I EarI Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I KspI MaeI MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TfiI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AflIII 524 ACATGT A^C[GA][CT]GT Aor51HI 27 AGCGCT AGC^GCT AspHI 654 GTGCTC G[AT]GC[AT]^C AvaII 180 GGTCC G^G[AT]CC BamHI 1161 GGATCC G^GATCC BanII 68 GGGCTC G[GA]GC[CT]^C BbeI 436 GGCGCC GGCGC^C BbrPI 774 CACGTG CAC^GTG BbsI 958 GAAGAC GAAGAC(2/6) BglI 928 GCCTGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 958 GAAGAC GAAGAC(2/6) BsaWI 388 ACCGGA [AT]^CCGG[AT] BsiEI 17 CGGCCG CG[GA][CT]^CG BsiHKAI 654 GTGCTC G[AT]GC[AT]^C BsmAI 764 GAGAC GTCTC(1/5) BsmBI 764 GAGACG CGTCTC(1/5) BspLU11I 524 ACATGT A^CATGT BsrDI 270 CATTGC GCAATG(2/0) BsrFI 1116 GCCGGT [GA]^CCGG[CT] BstYI 1161 GGATCC [GA]^GATC[CT] Cfr10I 1116 GCCGGT [GA]^CCGG[CT] DraI 1033 TTTAAA TTT^AAA DrdI 739 GACTGCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 669 CCGTGG C^C[GA][CT]GG EaeI 17 CGGCCG [CT]^GGCC[GA] EagI 17 CGGCCG C^GGCCG EclXI 17 CGGCCG C^GGCCG Eco47III 27 AGCGCT AGC^GCT Eco52I 17 CGGCCG C^GGCCG EcoRI 293 GAATTC G^AATTC FauI 492 GCGGG CCCGC(4/6) KasI 436 GGCGCC G^GCGCC MflI 1161 GGATCC [GA]^GATC[CT] MspA1I 555 CAGCTG C[AC]G^C[GT]G NarI 436 GGCGCC GG^CGCC NspI 524 ACATGT [GA]CATG^[CT] PflMI 246 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 774 CACGTG CAC^GTG PmlI 774 CACGTG CAC^GTG PvuII 555 CAGCTG CAG^CTG SfoI 436 GGCGCC GGC^GCC SmaI 728 CCCGGG CCC^GGG SspI 1063 AATATT AAT^ATT TaqI 529 TCGA T^CGA TthHB8I 529 TCGA T^CGA Van91I 246 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 1161 GGATCC [GA]^GATC[CT] XmaCI 728 CCCGGG C^CCGGG XmaI 728 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcyI 436 GGCGCC G[GA]^CG[CT]C 964 GGCGTC AfaI 333 GTAC GT^AC 822 GTAC AspI 496 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC 961 GACGGCGTC AvaI 728 CCCGGG C^[CT]CG[GA]G 841 CCCGAG BanI 436 GGCGCC G^G[CT][GA]CC 862 GGTGCC BpmI 151 CTCCAG CTGGAG(16/14) 73 CTGGAG BsaAI 774 CACGTG [CT]AC^GT[GA] 819 CACGTA BsaHI 436 GGCGCC G[GA]^CG[CT]C 964 GGCGTC BsoBI 728 CCCGGG C^[CT]CG[GA]G 841 CCCGAG BssSI 99 CACGAG CACGAG(-5/-1) 915 CTCGTG Cac8I 42 GCTGGC GC[ACGT]^[ACGT]GC 712 GCAGGC DraII 723 AGGCCCC [GA]G^G[ACGT]CC[CT] 990 AGGGCCT EcoO109I 723 AGGCCCC [GA]G^G[ACGT]CC[CT] 990 AGGGCCT EcoT14I 93 CCAAGG C^C[AT][AT]GG 183 CCTTGG HgaI 920 GCGTC GACGC(5/10) 965 GCGTC Hin1I 436 GGCGCC G[GA]^CG[CT]C 964 GGCGTC MslI 525 CATGTCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 867 CACCTGCGTG PflFI 496 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC 961 GACGGCGTC PstI 310 CTGCAG CTGCA^G 710 CTGCAG RsaI 333 GTAC GT^AC 822 GTAC SfaNI 376 GATGC GCATC(5/9) 482 GCATC SfcI 310 CTGCAG C^T[GA][CT]AG 710 CTGCAG StyI 93 CCAAGG C^C[AT][AT]GG 183 CCTTGG Tth111I 496 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC 961 GACGGCGTC ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AciI AcsI AluI AlwI AlwNI ApoI BbvI BcnI BmyI BsaJI BseRI BsgI BsiYI BslI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI CfoI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI Tru9I TseI Tsp45I Tsp509I TspRI XcmI