(Circular) (Six-base) MAPSORT of: pFN21AE2776.seq Check: 9648 from: 1 to: 5920 pFN21AE2776 5920 bp With 182 enzymes: SgfI * February 20, 2021 22:13 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2801 2882 2801 Size: 81 5839 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3730 278 Size: 53 83 186 936 2194 2468 Fragments arranged by size: 2468 2194 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3263 1937 Size: 1326 4594 Acc65I G'GTAC_C Cuts at: 3241 3241 Size: 5920 AclI AA'CG_TT Cuts at: 1563 4047 4420 1563 Size: 2484 373 3063 Fragments arranged by size: 3063 2484 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2420 3983 5367 5635 1632 Size: 788 1563 1384 268 1917 Fragments arranged by size: 1917 1563 1384 788 268 AfeI AGC'GCT Cuts at: 2034 2034 Size: 5920 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5723 AflIII A'CryG_T Cuts at: 1203 2529 4819 5796 1203 Size: 1326 2290 977 1327 Fragments arranged by size: 2290 1327 1326 977 AgeI A'CCGG_T Cuts at: 4751 4751 Size: 5920 AhdI GACnn_n'nnGTC Cuts at: 4649 4649 Size: 5920 AleI CACnn'nnGTG Cuts at: 1166 2876 1166 Size: 1710 4210 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5920 AlwNI CAG_nnn'CTG Cuts at: 2632 2694 3178 3663 5235 2632 Size: 62 484 485 1572 3317 Fragments arranged by size: 3317 1572 485 484 62 ApaLI G'TGCA_C Cuts at: 3977 5133 3977 Size: 1156 4764 ApoI r'AATT_y Cuts at: 1493 2194 2298 2347 2419 2762 3035 3229 3455 Size: 701 104 49 72 343 273 194 226 Cuts at: 3455 3607 5716 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 701 343 273 226 194 152 104 72 49 AseI AT'TA_AT Cuts at: 160 1032 4474 160 Size: 872 3442 1606 Fragments arranged by size: 3442 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5920 AvaI C'yCGr_G Cuts at: 1870 1943 2733 2846 3245 1870 Size: 73 790 113 399 4545 Fragments arranged by size: 4545 790 399 113 73 BamHI G'GATC_C Cuts at: 3166 3250 3617 3166 Size: 84 367 5469 Fragments arranged by size: 5469 367 84 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2441 2867 3241 4696 618 Size: 325 217 627 654 426 374 1455 1842 Fragments arranged by size: 1842 1455 654 627 426 374 325 217 BanII G_rGCy'C Cuts at: 1391 2077 3239 1391 Size: 686 1162 4072 Fragments arranged by size: 4072 1162 686 BbeI G_GCGC'C Cuts at: 2445 2445 Size: 5920 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2970 928 Size: 971 1071 3878 Fragments arranged by size: 3878 1071 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4111 4145 1903 Size: 34 50 34 2090 34 3678 Fragments arranged by size: 3678 2090 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3812 5022 3812 Size: 1210 4710 BclI T'GATC_A Cuts at: 1548 1548 Size: 5920 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2939 4531 136 Size: 107 122 71 631 605 1267 1592 1525 Fragments arranged by size: 1592 1525 1267 631 605 122 107 71 BglII A'GATC_T Cuts at: 5915 5915 Size: 5920 BlpI GC'TnA_GC Cuts at: 3337 3337 Size: 5920 Bme1580I G_kGCm'C Cuts at: 1790 2132 2653 3981 5137 1790 Size: 342 521 1328 1156 2573 Fragments arranged by size: 2573 1328 1156 521 342 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5920 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2682 3651 4609 Size: 416 53 83 399 24 1265 969 958 Cuts at: 4609 442 Size: 1753 Fragments arranged by size: 1753 1265 969 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5920 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2091 2091 Size: 5920 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2099 2141 4580 1239 Size: 860 42 2439 2579 Fragments arranged by size: 2579 2439 860 42 Bpu10I CC'TnA_GC Cuts at: 2359 2359 Size: 5920 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4045 4910 5208 4045 Size: 865 298 4757 Fragments arranged by size: 4757 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4583 882 Size: 617 3084 2219 Fragments arranged by size: 3084 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2781 2826 3711 493 Size: 711 1577 45 885 2702 Fragments arranged by size: 2702 1577 885 711 45 BsaBI GATnn'nnATC Cuts at: 5914 5914 Size: 5920 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2442 2970 3727 4109 Size: 53 83 186 936 909 528 757 382 Cuts at: 4109 4744 275 Size: 635 1451 Fragments arranged by size: 1451 936 909 757 635 528 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2393 4353 4751 5025 5172 5583 2393 Size: 1960 398 274 147 411 2730 Fragments arranged by size: 2730 1960 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3035 3065 3077 3107 3139 Size: 30 165 30 1632 30 12 30 32 Cuts at: 3139 3169 1178 Size: 30 3929 Fragments arranged by size: 3929 1632 165 32 30 30 30 30 30 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2630 3147 3150 1186 Size: 1444 517 3 3956 Fragments arranged by size: 3956 1444 517 3 BseYI C'CCAG_C Cuts at: 2601 2652 5123 5619 2601 Size: 51 2471 496 2902 Fragments arranged by size: 2902 2471 496 51 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2234 2628 2707 3073 1163 Size: 573 498 394 79 366 4010 Fragments arranged by size: 4010 573 498 394 366 79 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2025 3370 4131 5159 1540 Size: 275 186 24 1345 761 1028 2301 Fragments arranged by size: 2301 1345 1028 761 275 186 24 BsiHKAI G_wGCw'C Cuts at: 2663 3981 4066 5137 2663 Size: 1318 85 1071 3446 Fragments arranged by size: 3446 1318 1071 85 BsmI GAATG_Cn' Cuts at: 3431 3524 3431 Size: 93 5827 BsmBI CGTCTCn'nnnn_ Cuts at: 2763 2763 Size: 5920 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2077 2132 2653 2663 3239 3981 4066 Size: 399 287 55 521 10 576 742 85 Cuts at: 4066 5137 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 576 521 399 287 85 55 10 BspEI T'CCGG_A Cuts at: 5583 5583 Size: 5920 BspHI T'CATG_A Cuts at: 3809 5539 3809 Size: 1730 4190 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2322 2801 2882 3275 844 Size: 1478 479 81 393 3489 Fragments arranged by size: 3489 1478 479 393 81 BsrBI CCG'CTC Cuts at: 1394 1823 3807 1394 Size: 429 1984 3507 Fragments arranged by size: 3507 1984 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2274 4415 4589 65 Size: 1183 1026 2141 174 1396 Fragments arranged by size: 2141 1396 1183 1026 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3121 4564 4751 1700 Size: 110 1311 1443 187 2869 Fragments arranged by size: 2869 1443 1311 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5920 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5920 BssSI C'ACGA_G Cuts at: 2104 2920 3980 4992 2104 Size: 816 1060 1012 3032 Fragments arranged by size: 3032 1060 1012 816 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5920 BstBI TT'CG_AA Cuts at: 4724 4724 Size: 5920 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5920 BstYI r'GATC_y Cuts at: 1972 3166 3250 3617 4002 4019 5471 5557 5569 Size: 1194 84 367 385 17 1452 86 12 Cuts at: 5569 5915 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1194 385 367 346 86 84 17 12 BtgI C'CryG_G Cuts at: 513 1065 2674 513 Size: 552 1609 3759 Fragments arranged by size: 3759 1609 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5181 Fragments arranged by size: 5181 683 56 BtsI GCAGTG_nn' Cuts at: 2518 2663 3436 4230 4250 2518 Size: 145 773 794 20 4188 Fragments arranged by size: 4188 794 773 145 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5920 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5885 DraI TTT'AAA Cuts at: 3040 3224 3040 Size: 184 5736 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2750 4927 809 Size: 507 1434 2177 1802 Fragments arranged by size: 2177 1802 1434 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2022 3367 4256 Size: 54 1425 50 275 18 192 1345 889 Cuts at: 4256 5885 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1345 889 275 192 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2022 3367 1537 Size: 275 210 1345 4090 Fragments arranged by size: 4090 1345 275 210 EarI CTCTTCn'nnn_ Cuts at: 1353 3850 1353 Size: 2497 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4507 4881 5027 387 Size: 1064 138 2918 374 146 1280 Fragments arranged by size: 2918 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2099 2141 2420 3983 4580 5367 5635 Size: 393 467 42 279 1563 597 787 268 Cuts at: 5635 1239 Size: 1524 Fragments arranged by size: 1563 1524 787 597 467 393 279 268 42 EcoO109I rG'GnC_Cy Cuts at: 2729 2996 2729 Size: 267 5653 EcoRI G'AATT_C Cuts at: 2298 3229 3607 2298 Size: 931 378 4611 Fragments arranged by size: 4611 931 378 FspI TGC'GCA Cuts at: 4426 4426 Size: 5920 HaeII r_GCGC'y Cuts at: 1678 2036 2045 2445 2671 5067 1678 Size: 358 9 400 226 2396 2531 Fragments arranged by size: 2531 2396 400 358 226 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2903 2935 3035 3067 4561 Size: 32 135 32 1331 32 100 32 1494 Cuts at: 4561 4593 4635 4667 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1494 1331 135 100 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3264 3510 669 Size: 988 281 1326 246 3079 Fragments arranged by size: 3079 1326 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5920 HpaI GTT'AAC Cuts at: 3510 3510 Size: 5920 KasI G'GCGC_C Cuts at: 2441 2441 Size: 5920 KpnI G_GTAC'C Cuts at: 3245 3245 Size: 5920 MfeI C'AATT_G Cuts at: 3519 3519 Size: 5920 MluI A'CGCG_T Cuts at: 5796 5796 Size: 5920 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5034 5218 1305 Size: 3729 184 2007 Fragments arranged by size: 3729 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4098 Fragments arranged by size: 4098 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2534 2876 3878 4237 4396 518 Size: 648 1368 342 1002 359 159 2042 Fragments arranged by size: 2042 1368 1002 648 359 342 159 MspA1I CmG'CkG Cuts at: 1606 2562 3336 4013 5161 5406 1606 Size: 956 774 677 1148 245 2120 Fragments arranged by size: 2120 1148 956 774 677 245 NarI GG'CG_CC Cuts at: 2442 2442 Size: 5920 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5368 NdeI CA'TA_TG Cuts at: 387 387 Size: 5920 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5920 NotI GC'GGCC_GC Cuts at: 3367 3367 Size: 5920 NspI r_CATG'y Cuts at: 1132 1229 2533 3278 4823 1132 Size: 97 1304 745 1545 2229 Fragments arranged by size: 2229 1545 1304 745 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5920 PciI A'CATG_T Cuts at: 2529 4819 2529 Size: 2290 3630 PflMI CCAn_nnn'nTGG Cuts at: 1108 2257 1108 Size: 1149 4771 PfoI T'CCnGG_A Cuts at: 2015 2250 2015 Size: 235 5685 PmeI GTTT'AAAC Cuts at: 3224 3224 Size: 5920 PmlI CAC'GTG Cuts at: 2781 2781 Size: 5920 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3989 4021 5502 5534 3989 Size: 32 1481 32 4375 Fragments arranged by size: 4375 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5920 PsiI TTA'TAA Cuts at: 3490 3490 Size: 5920 PstI C_TGCA'G Cuts at: 830 2319 2719 3272 830 Size: 1489 400 553 3478 Fragments arranged by size: 3478 1489 553 400 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5920 PvuII CAG'CTG Cuts at: 2562 2562 Size: 5920 SalI G'TCGA_C Cuts at: 1936 3262 1936 Size: 1326 4594 SbfI CC_TGCA'GG Cuts at: 3272 3272 Size: 5920 ScaI AGT'ACT Cuts at: 4168 5880 4168 Size: 1712 4208 SfcI C'TryA_G Cuts at: 826 1045 2315 2715 3268 4403 5084 5275 826 Size: 219 1270 400 553 1135 681 191 1471 Fragments arranged by size: 1471 1270 1135 681 553 400 219 191 SfoI GGC'GCC Cuts at: 2443 2443 Size: 5920 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5920 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5920 SmaI CCC'GGG Cuts at: 1872 2735 3247 1872 Size: 863 512 4545 Fragments arranged by size: 4545 863 512 SmlI C'TyrA_G Cuts at: 820 1017 1943 4024 4925 5187 820 Size: 197 926 2081 901 262 1553 Fragments arranged by size: 2081 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3711 493 Size: 3218 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5920 SphI G_CATG'C Cuts at: 1132 3278 1132 Size: 2146 3774 SspI AAT'ATT Cuts at: 5 52 3070 3844 5 Size: 47 3018 774 2081 Fragments arranged by size: 3018 2081 774 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5920 StyI C'CwwG_G Cuts at: 513 1065 2098 2188 3359 513 Size: 552 1033 90 1171 3074 Fragments arranged by size: 3074 1171 1033 552 90 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3947 3964 4117 4302 4747 325 Size: 1255 2367 17 153 185 445 1498 Fragments arranged by size: 2367 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2337 4166 5878 Size: 274 80 33 51 1443 360 1829 1712 Cuts at: 5878 96 Size: 138 Fragments arranged by size: 1829 1712 1443 360 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3139 3171 5880 5912 3139 Size: 32 2709 32 3147 Fragments arranged by size: 3147 2709 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2504 2969 1316 Size: 1188 465 4267 Fragments arranged by size: 4267 1188 465 XbaI T'CTAG_A Cuts at: 3256 3256 Size: 5920 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2254 2258 2830 2254 Size: 4 572 5344 Fragments arranged by size: 5344 572 4 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5920 XmaI C'CCGG_G Cuts at: 1870 2733 3245 1870 Size: 863 512 4545 Fragments arranged by size: 4545 863 512 XmnI GAAnn'nnTTC Cuts at: 4049 4049 Size: 5920 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3728 276 Size: 53 83 186 936 2194 2468 Fragments arranged by size: 2468 2194 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI