################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## AatII Acc65I AccII AgeI AhdI AocI Aor51HI AscI Asp718I AspEI AvrII BbeI BbrPI BcgI BciVI BcnI BglI BlnI BsiWI BspDI BsrFI BssHII BstEII BstPI BstUI Bsu36I Cfr10I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I FseI KasI KpnI KspI MluI MvnI NaeI NarI NciI NgoMIV NotI NruI PacI PaeR7I PinAI PmaCI PmeI PmlI PvuI RsrII SacI SacII SalI SbfI SfiI SfoI SgrAI SmaI SnaBI Sse8387I SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccIII 4340 TCCGGA T^CCGGA AclI 3050 AACGTT AA^CGTT AcyI 5763 GGCGTC G[GA]^CG[CT]C Alw44I 6239 GTGCAC G^TGCAC ApaLI 6239 GTGCAC G^TGCAC AspI 2460 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 3330 CTCGGG C^[CT]CG[GA]G AviII 7169 TGCGCA TGC^GCA BanI 6636 GGTGCC G^G[CT][GA]CC BsaHI 5763 GGCGTC G[GA]^CG[CT]C BsaI 5894 GAGACC GGTCTC(1/5) BseAI 4340 TCCGGA T^CCGGA BsiEI 787 CGACCG CG[GA][CT]^CG BsmBI 3574 GAGACG CGTCTC(1/5) BsoBI 3330 CTCGGG C^[CT]CG[GA]G BspEI 4340 TCCGGA T^CCGGA BsrBI 6175 GAGCGG CCGCTC(-3/-3) Bst1107I 7025 GTATAC GTA^TAC BstBI 1285 TTCGAA TT^CGAA BstZ17I 7025 GTATAC GTA^TAC DraIII 5270 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 3782 GACAAAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 5732 GAATTC G^AATTC EcoRV 111 GATATC GAT^ATC FauI 6177 GCGGG CCCGC(4/6) FspI 7169 TGCGCA TGC^GCA HaeII 246 GGCGCT [GA]GCGC^[CT] HgaI 5764 GCGTC GACGC(5/10) Hin1I 5763 GGCGTC G[GA]^CG[CT]C HincII 2751 GTTAAC GT[CT]^[GA]AC HindII 2751 GTTAAC GT[CT]^[GA]AC HpaI 2751 GTTAAC GTT^AAC MroI 4340 TCCGGA T^CCGGA NheI 1648 GCTAGC G^CTAGC NspV 1285 TTCGAA TT^CGAA PflFI 2460 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 5535 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 3050 AACGTT AA^CGTT SfuI 1285 TTCGAA TT^CGAA SspI 6199 AATATT AAT^ATT StuI 5119 AGGCCT AGG^CCT Tth111I 2460 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 2640 CCAGTTGGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AflII 2520 CTTAAG C^TTAAG 4747 CTTAAG AflIII 4101 ACATGT A^C[GA][CT]GT 4903 ACATGT ApaI 5445 GGGCCC GGGCC^C 6805 GGGCCC AseI 4693 ATTAAT AT^TAAT 5395 ATTAAT AsnI 4693 ATTAAT AT^TAAT 5395 ATTAAT BalI 2471 TGGCCA TGG^CCA 4268 TGGCCA BamHI 5208 GGATCC G^GATCC 6348 GGATCC BanII 5445 GGGCCC G[GA]GC[CT]^C 6805 GGGCCC BbvCI 2659 CCTCAGC CCTCAGC(-5/-2) 2293 GCTGAGG BclI 797 TGATCA T^GATCA 5300 TGATCA BfrI 2520 CTTAAG C^TTAAG 4747 CTTAAG BglII 303 AGATCT A^GATCT 2823 AGATCT BsaAI 600 CACGTA [CT]AC^GT[GA] 7051 TACGTG BspLU11I 4101 ACATGT A^CATGT 4903 ACATGT BspMI 6060 ACCTGC ACCTGC(4/8) 4642 GCAGGT BssSI 2972 CACGAG CACGAG(-5/-1) 6452 CTCGTG BstXI 5744 CCAATTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 6005 CCAAAATAATGG CfoI 247 GCGC GCG^C 7170 GCGC DsaI 2474 CCATGG C^C[GA][CT]GG 3182 CCATGG EaeI 2471 TGGCCA [CT]^GGCC[GA] 4268 TGGCCA FbaI 797 TGATCA T^GATCA 5300 TGATCA HhaI 247 GCGC GCG^C 7170 GCGC HinP1I 247 GCGC G^CGC 7170 GCGC HindIII 628 AAGCTT A^AGCTT 6329 AAGCTT MluNI 2471 TGGCCA TGG^CCA 4268 TGGCCA MscI 2471 TGGCCA TGG^CCA 4268 TGGCCA NcoI 2474 CCATGG C^CATGG 3182 CCATGG NdeI 257 CATATG CA^TATG 5296 CATATG PpuMI 906 AGGACCC [GA]G^G[AT]CC[CT] 1098 AGGACCT PshBI 4693 ATTAAT AT^TAAT 5395 ATTAAT SapI 10 GAAGAGC GCTCTTC(1/4) 2256 GCTCTTC ScaI 479 AGTACT AGT^ACT 694 AGTACT SpeI 2436 ACTAGT A^CTAGT 4216 ACTAGT SphI 4887 GCATGC GCATG^C 5699 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BbsI BbvI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaBI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspHI BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CelII Cfr13I DdeI DpnII DraI DraII EarI EcoNI EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MfeI MflI MnlI MseI MslI MspA1I MspI MunI MvaI MwoI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XhoII XmnI