(Circular) (Six-base) MAPSORT of: pFN21AE2748.seq Check: 8663 from: 1 to: 12100 pFN21AE2748 12100 bp With 182 enzymes: SgfI * June 20, 2019 11:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 8074 8074 Size: 12100 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 9910 278 Size: 53 83 186 936 8374 2468 Fragments arranged by size: 8374 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2215 2434 7251 9031 9443 1937 Size: 278 219 4817 1780 412 4594 Fragments arranged by size: 4817 4594 1780 412 278 219 Acc65I G'GTAC_C Cuts at: 9421 9421 Size: 12100 AclI AA'CG_TT Cuts at: 1563 5056 10227 10600 1563 Size: 3493 5171 373 3063 Fragments arranged by size: 5171 3493 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2688 2961 3181 3350 3774 3963 5306 5962 Size: 1056 273 220 169 424 189 1343 656 Cuts at: 5962 6245 6746 6825 6866 7341 7694 7889 8790 Size: 283 501 79 41 475 353 195 901 Cuts at: 8790 10163 11547 11815 1632 Size: 1373 1384 268 1917 Fragments arranged by size: 1917 1384 1373 1343 1056 901 656 501 475 424 353 283 273 268 220 195 189 169 79 41 AflII C'TTAA_G Cuts at: 820 1017 4525 6752 820 Size: 197 3508 2227 6168 Fragments arranged by size: 6168 3508 2227 197 AflIII A'CryG_T Cuts at: 1203 6106 6908 10999 11976 1203 Size: 4903 802 4091 977 1327 Fragments arranged by size: 4903 4091 1327 977 802 AgeI A'CCGG_T Cuts at: 10931 10931 Size: 12100 AhdI GACnn_n'nnGTC Cuts at: 10829 10829 Size: 12100 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 12100 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4045 5136 6752 1184 Size: 2861 1091 1616 6532 Fragments arranged by size: 6532 2861 1616 1091 AlwNI CAG_nnn'CTG Cuts at: 3532 3609 4384 5653 6769 7622 9193 9843 11415 Size: 77 775 1269 1116 853 1571 650 1572 Cuts at: 11415 3532 Size: 4217 Fragments arranged by size: 4217 1572 1571 1269 1116 853 775 650 77 ApaI G_GGCC'C Cuts at: 7454 8814 7454 Size: 1360 10740 ApaLI G'TGCA_C Cuts at: 8244 10157 11313 8244 Size: 1913 1156 9031 Fragments arranged by size: 9031 1913 1156 ApoI r'AATT_y Cuts at: 1493 2083 4174 4610 5116 6366 6477 6716 7069 Size: 590 2091 436 506 1250 111 239 353 Cuts at: 7069 7737 8912 9044 9409 9635 9787 11896 1493 Size: 668 1175 132 365 226 152 2109 1697 Fragments arranged by size: 2109 2091 1697 1250 1175 668 590 506 436 365 353 239 226 152 132 111 AseI AT'TA_AT Cuts at: 160 1032 6699 7401 10654 160 Size: 872 5667 702 3253 1606 Fragments arranged by size: 5667 3253 1606 872 702 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 12100 AvaI C'yCGr_G Cuts at: 1870 1943 5335 9425 1870 Size: 73 3392 4090 4545 Fragments arranged by size: 4545 4090 3392 73 BamHI G'GATC_C Cuts at: 7213 8353 9430 9797 7213 Size: 1140 1077 367 9516 Fragments arranged by size: 9516 1140 1077 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 8641 9421 10876 618 Size: 325 217 627 6854 780 1455 1842 Fragments arranged by size: 6854 1842 1455 780 627 325 217 BanII G_rGCy'C Cuts at: 1391 7454 8814 9419 1391 Size: 6063 1360 605 4072 Fragments arranged by size: 6063 4072 1360 605 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2561 2869 3720 4010 4610 7522 7802 Size: 971 662 308 851 290 600 2912 280 Cuts at: 7802 9117 9214 928 Size: 1315 97 3814 Fragments arranged by size: 3814 2912 1315 971 851 662 600 308 290 280 97 BbvCI CC'TCA_GC Cuts at: 4299 4665 4299 Size: 366 11734 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 10291 10325 1903 Size: 34 8354 34 3678 Fragments arranged by size: 8354 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 9992 11202 9992 Size: 1210 10890 BclI T'GATC_A Cuts at: 1548 2802 7305 1548 Size: 1254 4503 6343 Fragments arranged by size: 6343 4503 1254 BfrBI ATG'CAT Cuts at: 2873 6236 8114 8212 2873 Size: 3363 1878 98 6761 Fragments arranged by size: 6761 3363 1878 98 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 10711 136 Size: 107 122 71 631 605 9039 1525 Fragments arranged by size: 9039 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2308 4828 12095 2308 Size: 2520 7267 2313 Fragments arranged by size: 7267 2520 2313 BlpI GC'TnA_GC Cuts at: 6523 6897 8285 9517 6523 Size: 374 1388 1232 9106 Fragments arranged by size: 9106 1388 1232 374 Bme1580I G_kGCm'C Cuts at: 1790 2557 7454 7749 8226 8248 8814 9150 10161 Size: 767 4897 295 477 22 566 336 1011 Cuts at: 10161 11317 1790 Size: 1156 2573 Fragments arranged by size: 4897 2573 1156 1011 767 566 477 336 295 22 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 12100 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3520 4280 4497 Size: 416 53 83 399 24 2103 760 217 Cuts at: 4497 8352 9831 10789 442 Size: 3855 1479 958 1753 Fragments arranged by size: 3855 2103 1753 1479 958 760 416 399 217 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3657 1056 Size: 2601 9499 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2042 2080 3399 3551 3681 4361 5686 6042 Size: 803 38 1319 152 130 680 1325 356 Cuts at: 6042 6107 7803 8849 9332 10760 1239 Size: 65 1696 1046 483 1428 2579 Fragments arranged by size: 2579 1696 1428 1325 1319 1046 803 680 483 356 152 130 65 38 Bpu10I CC'TnA_GC Cuts at: 2292 2837 3096 3623 4299 4665 2292 Size: 545 259 527 676 366 9727 Fragments arranged by size: 9727 676 545 527 366 259 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2701 4261 5389 7444 8643 10225 11090 11388 2701 Size: 1560 1128 2055 1199 1582 865 298 3413 Fragments arranged by size: 3413 2055 1582 1560 1199 1128 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7893 10763 882 Size: 617 6394 2870 2219 Fragments arranged by size: 6394 2870 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2607 9058 9891 493 Size: 711 1403 6451 833 2702 Fragments arranged by size: 6451 2702 1403 833 711 BsaBI GATnn'nnATC Cuts at: 2807 5292 5565 12094 2807 Size: 2485 273 6529 2813 Fragments arranged by size: 6529 2813 2485 273 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 7769 9907 10289 10924 Size: 53 83 186 936 6236 2138 382 635 Cuts at: 10924 275 Size: 1451 Fragments arranged by size: 6236 2138 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2794 6282 6345 10533 10931 11205 11352 11763 2794 Size: 3488 63 4188 398 274 147 411 3131 Fragments arranged by size: 4188 3488 3131 411 398 274 147 63 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4359 4389 5229 5259 5785 Size: 30 165 30 2956 30 840 30 526 Cuts at: 5785 5815 6096 6126 8822 8852 1178 Size: 30 281 30 2696 30 4426 Fragments arranged by size: 4426 2956 2696 840 526 281 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 5105 5704 6234 1186 Size: 3919 599 530 7052 Fragments arranged by size: 7052 3919 599 530 BseYI C'CCAG_C Cuts at: 5357 7764 11303 11799 5357 Size: 2407 3539 496 5658 Fragments arranged by size: 5658 3539 2407 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2729 4379 4500 5575 6414 6951 7084 Size: 573 993 1650 121 1075 839 537 133 Cuts at: 7084 9151 1163 Size: 2067 4112 Fragments arranged by size: 4112 2067 1650 1075 993 839 573 537 133 121 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2795 9550 10311 11339 1540 Size: 275 186 794 6755 761 1028 2301 Fragments arranged by size: 6755 2301 1028 794 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4277 6114 6622 8248 9213 10161 10246 11317 4277 Size: 1837 508 1626 965 948 85 1071 5060 Fragments arranged by size: 5060 1837 1626 1071 965 948 508 85 BsmI GAATG_Cn' Cuts at: 2543 4396 5404 5626 9611 9704 2543 Size: 1853 1008 222 3985 93 4939 Fragments arranged by size: 4939 3985 1853 1008 222 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5573 5573 Size: 12100 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2557 4277 6114 6622 7454 7749 8226 Size: 399 767 1720 1837 508 832 295 477 Cuts at: 8226 8248 8814 9150 9213 9419 10161 10246 11317 Size: 22 566 336 63 206 742 85 1071 Cuts at: 11317 1391 Size: 2174 Fragments arranged by size: 2174 1837 1720 1071 832 767 742 566 508 477 399 336 295 206 85 63 22 BspEI T'CCGG_A Cuts at: 6345 11763 6345 Size: 5418 6682 BspHI T'CATG_A Cuts at: 3739 5749 7608 9989 11719 3739 Size: 2010 1859 2381 1730 4120 Fragments arranged by size: 4120 2381 2010 1859 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 6638 8074 9455 844 Size: 5794 1436 1381 3489 Fragments arranged by size: 5794 3489 1436 1381 BsrBI CCG'CTC Cuts at: 1394 1823 8182 9987 1394 Size: 429 6359 1805 3507 Fragments arranged by size: 6359 3507 1805 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2375 3003 3572 4029 4293 4991 5137 Size: 1183 1127 628 569 457 264 698 146 Cuts at: 5137 7491 8076 8933 10595 10769 65 Size: 2354 585 857 1662 174 1396 Fragments arranged by size: 2354 1662 1396 1183 1127 857 698 628 585 569 457 264 174 146 BsrFI r'CCGG_y Cuts at: 1700 1810 10744 10931 1700 Size: 110 8934 187 2869 Fragments arranged by size: 8934 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2728 5044 6357 8746 96 Size: 2632 2316 1313 2389 3450 Fragments arranged by size: 3450 2632 2389 2316 1313 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 12100 BssSI C'ACGA_G Cuts at: 4977 8457 10160 11172 4977 Size: 3480 1703 1012 5905 Fragments arranged by size: 5905 3480 1703 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2354 5110 7940 8252 9154 9193 794 Size: 1560 2756 2830 312 902 39 3701 Fragments arranged by size: 3701 2830 2756 1560 902 312 39 BstBI TT'CG_AA Cuts at: 3291 10904 3291 Size: 7613 4487 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 12100 BstXI CCAn_nnnn'nTGG Cuts at: 7756 8017 7756 Size: 261 11839 BstYI r'GATC_y Cuts at: 1972 2308 2808 3052 3175 3618 3938 4132 4828 Size: 336 500 244 123 443 320 194 696 Cuts at: 4828 5566 6062 7213 8353 9430 9797 10182 10199 Size: 738 496 1151 1140 1077 367 385 17 Cuts at: 10199 11651 11737 11749 12095 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1151 1140 1077 738 696 500 496 443 385 367 346 336 320 244 194 123 86 17 12 BstZ17I GTA'TAC Cuts at: 9032 9032 Size: 12100 BtgI C'CryG_G Cuts at: 513 1065 4479 5187 513 Size: 552 3414 708 7426 Fragments arranged by size: 7426 3414 708 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 11361 Fragments arranged by size: 11361 683 56 BtsI GCAGTG_nn' Cuts at: 2674 3350 4034 5266 5563 6939 7285 7322 8472 Size: 676 684 1232 297 1376 346 37 1150 Cuts at: 8472 8572 8778 9616 10410 10430 2674 Size: 100 206 838 794 20 4344 Fragments arranged by size: 4344 1376 1232 1150 838 794 684 676 346 297 206 100 37 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 12100 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 12065 DraI TTT'AAA Cuts at: 2625 7650 9049 9404 2625 Size: 5025 1399 355 5321 Fragments arranged by size: 5321 5025 1399 355 DraIII CAC_nnn'GTG Cuts at: 7280 7280 Size: 12100 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5793 11107 809 Size: 507 4477 5314 1802 Fragments arranged by size: 5314 4477 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4476 6273 9547 Size: 54 1425 50 275 18 2646 1797 3274 Cuts at: 9547 10436 12065 8 Size: 889 1629 43 Fragments arranged by size: 3274 2646 1797 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 9547 1537 Size: 275 7735 4090 Fragments arranged by size: 7735 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2010 2346 2762 3027 4268 4537 4673 5571 Size: 657 336 416 265 1241 269 136 898 Cuts at: 5571 5751 5761 6497 6801 6885 9351 10030 1353 Size: 180 10 736 304 84 2466 679 3423 Fragments arranged by size: 3423 2466 1241 898 736 679 657 416 336 304 269 265 180 136 84 10 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 10687 11061 11207 387 Size: 1064 138 9098 374 146 1280 Fragments arranged by size: 9098 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 2080 2688 2961 3181 3350 3399 Size: 393 410 38 608 273 220 169 49 Cuts at: 3399 3551 3681 3774 3963 4361 5306 5686 5962 Size: 152 130 93 189 398 945 380 276 Cuts at: 5962 6042 6107 6245 6746 6825 6866 7341 7694 Size: 80 65 138 501 79 41 475 353 Cuts at: 7694 7803 7889 8790 8849 9332 10163 10760 11547 Size: 109 86 901 59 483 831 597 787 Cuts at: 11547 11815 1239 Size: 268 1524 Fragments arranged by size: 1524 945 901 831 787 608 597 501 483 475 410 398 393 380 353 276 273 268 220 189 169 152 138 130 109 93 86 80 79 65 59 49 41 38 EcoNI CCTnn'n_nnAGG Cuts at: 3099 3626 4574 3099 Size: 527 948 10625 Fragments arranged by size: 10625 948 527 EcoO109I rG'GnC_Cy Cuts at: 2912 3104 7451 8810 8811 2912 Size: 192 4347 1359 1 6201 Fragments arranged by size: 6201 4347 1359 192 1 EcoRI G'AATT_C Cuts at: 7737 9409 9787 7737 Size: 1672 378 10050 Fragments arranged by size: 10050 1672 378 EcoRV GAT'ATC Cuts at: 2118 2118 Size: 12100 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2669 4151 5924 6605 7589 2669 Size: 1482 1773 681 984 7180 Fragments arranged by size: 7180 1773 1482 984 681 FspI TGC'GCA Cuts at: 9176 10606 9176 Size: 1430 10670 HaeII r_GCGC'y Cuts at: 1678 2255 11247 1678 Size: 577 8992 2531 Fragments arranged by size: 8992 2531 577 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2102 2134 2386 2418 3547 Size: 32 135 32 530 32 252 32 1129 Cuts at: 3547 3579 4554 4586 5229 5261 6545 6577 7153 Size: 32 975 32 643 32 1284 32 576 Cuts at: 7153 7185 8045 8077 8259 8291 10741 10773 10815 Size: 32 860 32 182 32 2450 32 42 Cuts at: 10815 10847 1373 Size: 32 2626 Fragments arranged by size: 2626 2450 1284 1129 975 860 643 576 530 252 182 135 42 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4758 9444 9690 669 Size: 988 281 2820 4686 246 3079 Fragments arranged by size: 4686 3079 2820 988 281 246 HindIII A'AGCT_T Cuts at: 748 2633 8334 748 Size: 1885 5701 4514 Fragments arranged by size: 5701 4514 1885 HpaI GTT'AAC Cuts at: 4758 9690 4758 Size: 4932 7168 KpnI G_GTAC'C Cuts at: 9425 9425 Size: 12100 MfeI C'AATT_G Cuts at: 2064 3894 7935 7944 9699 2064 Size: 1830 4041 9 1755 4465 Fragments arranged by size: 4465 4041 1830 1755 9 MluI A'CGCG_T Cuts at: 11976 11976 Size: 12100 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2490 3153 3423 4072 7191 7241 11214 11398 Size: 1185 663 270 649 3119 50 3973 184 Cuts at: 11398 1305 Size: 2007 Fragments arranged by size: 3973 3119 2007 1185 663 649 270 184 50 MscI TGG'CCA Cuts at: 10 64 1489 1832 4478 6275 10 Size: 54 1425 343 2646 1797 5835 Fragments arranged by size: 5835 2646 1797 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2363 3044 5682 6323 6461 7460 7501 Size: 648 1197 681 2638 641 138 999 41 Cuts at: 7501 10058 10417 10576 518 Size: 2557 359 159 2042 Fragments arranged by size: 2638 2557 2042 1197 999 681 648 641 359 159 138 41 MspA1I CmG'CkG Cuts at: 1606 3409 3884 8281 8881 9516 10193 11341 11586 Size: 1803 475 4397 600 635 677 1148 245 Cuts at: 11586 1606 Size: 2120 Fragments arranged by size: 4397 2120 1803 1148 677 635 600 475 245 NcoI C'CATG_G Cuts at: 513 1065 4479 5187 513 Size: 552 3414 708 7426 Fragments arranged by size: 7426 3414 708 552 NdeI CA'TA_TG Cuts at: 387 2263 7302 387 Size: 1876 5039 5185 Fragments arranged by size: 5185 5039 1876 NheI G'CTAG_C Cuts at: 1052 3653 1052 Size: 2601 9499 NotI GC'GGCC_GC Cuts at: 9547 9547 Size: 12100 NsiI A_TGCA'T Cuts at: 2875 6238 8116 8214 2875 Size: 3363 1878 98 6761 Fragments arranged by size: 6761 3363 1878 98 NspI r_CATG'y Cuts at: 1132 1229 2951 3359 4358 4625 4796 6110 6397 Size: 97 1722 408 999 267 171 1314 287 Cuts at: 6397 6460 6896 6912 6922 6966 7708 9458 11003 Size: 63 436 16 10 44 742 1750 1545 Cuts at: 11003 1132 Size: 2229 Fragments arranged by size: 2229 1750 1722 1545 1314 999 742 436 408 287 267 171 97 63 44 16 10 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 12100 PciI A'CATG_T Cuts at: 6106 6908 10999 6106 Size: 802 4091 7207 Fragments arranged by size: 7207 4091 802 PflMI CCAn_nnn'nTGG Cuts at: 1108 2103 2998 7240 1108 Size: 995 895 4242 5968 Fragments arranged by size: 5968 4242 995 895 PfoI T'CCnGG_A Cuts at: 5083 5702 6058 6140 5083 Size: 619 356 82 11043 Fragments arranged by size: 11043 619 356 82 PmeI GTTT'AAAC Cuts at: 9404 9404 Size: 12100 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 10169 10201 11682 11714 10169 Size: 32 1481 32 10555 Fragments arranged by size: 10555 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2912 3104 2912 Size: 192 11908 PshAI GACnn'nnGTC Cuts at: 1613 7544 1613 Size: 5931 6169 PsiI TTA'TAA Cuts at: 4745 9670 4745 Size: 4925 7175 PspOMI G'GGCC_C Cuts at: 7450 8810 7450 Size: 1360 10740 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5626 5658 5626 Size: 32 12068 PstI C_TGCA'G Cuts at: 830 5129 5668 7032 7558 9452 830 Size: 4299 539 1364 526 1894 3478 Fragments arranged by size: 4299 3478 1894 1364 539 526 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 12100 PvuII CAG'CTG Cuts at: 3409 3884 8281 3409 Size: 475 4397 7228 Fragments arranged by size: 7228 4397 475 SalI G'TCGA_C Cuts at: 1936 9442 1936 Size: 7506 4594 SapI GCTCTTCn'nnn_ Cuts at: 2010 4268 2010 Size: 2258 9842 SbfI CC_TGCA'GG Cuts at: 9452 9452 Size: 12100 ScaI AGT'ACT Cuts at: 2486 2701 10348 12060 2486 Size: 215 7647 1712 2526 Fragments arranged by size: 7647 2526 1712 215 SexAI A'CCwGG_T Cuts at: 2230 2428 4452 6657 2230 Size: 198 2024 2205 7673 Fragments arranged by size: 7673 2205 2024 198 SfcI C'TryA_G Cuts at: 826 1045 2435 2548 4116 5125 5664 6192 7028 Size: 219 1390 113 1568 1009 539 528 836 Cuts at: 7028 7554 8164 9448 10583 11264 11455 826 Size: 526 610 1284 1135 681 191 1471 Fragments arranged by size: 1568 1471 1390 1284 1135 1009 836 681 610 539 528 526 219 191 113 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 12100 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 12100 SmaI CCC'GGG Cuts at: 1872 9427 1872 Size: 7555 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2716 4276 4525 5368 6752 7423 Size: 197 926 773 1560 249 843 1384 671 Cuts at: 7423 8658 10204 11105 11367 820 Size: 1235 1546 901 262 1553 Fragments arranged by size: 1560 1553 1546 1384 1235 926 901 843 773 671 262 249 197 SnaBI TAC'GTA Cuts at: 493 9891 493 Size: 9398 2702 SpeI A'CTAG_T Cuts at: 152 4441 6221 152 Size: 4289 1780 6031 Fragments arranged by size: 6031 4289 1780 SphI G_CATG'C Cuts at: 1132 6896 7708 9458 1132 Size: 5764 812 1750 3774 Fragments arranged by size: 5764 3774 1750 812 SspI AAT'ATT Cuts at: 5 52 8206 10024 5 Size: 47 8154 1818 2081 Fragments arranged by size: 8154 2081 1818 47 StuI AGG'CCT Cuts at: 1517 7126 1517 Size: 5609 6491 StyI C'CwwG_G Cuts at: 513 1065 4479 5187 5424 7542 7671 9539 513 Size: 552 3414 708 237 2118 129 1868 3074 Fragments arranged by size: 3414 3074 2118 1868 708 552 237 129 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3852 6990 10127 10144 10297 10482 10927 Size: 1255 2272 3138 3137 17 153 185 445 Cuts at: 10927 325 Size: 1498 Fragments arranged by size: 3138 3137 2272 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2484 2699 2722 Size: 274 80 33 51 1443 507 215 23 Cuts at: 2722 2728 5035 5044 5143 6357 7092 8307 8746 Size: 6 2307 9 99 1214 735 1215 439 Cuts at: 8746 10346 12058 96 Size: 1600 1712 138 Fragments arranged by size: 2307 1712 1600 1443 1215 1214 735 507 439 274 215 138 99 80 51 33 23 9 6 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4359 4391 5206 5238 12060 12092 4359 Size: 32 815 32 6822 32 4367 Fragments arranged by size: 6822 4367 815 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4468 1316 Size: 3152 8948 XbaI T'CTAG_A Cuts at: 3115 5783 6778 9436 3115 Size: 2668 995 2658 5779 Fragments arranged by size: 5779 2668 2658 995 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4652 4652 Size: 12100 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 12100 XmaI C'CCGG_G Cuts at: 1870 9425 1870 Size: 7555 4545 XmnI GAAnn'nnTTC Cuts at: 3544 5319 6533 7590 8949 10229 3544 Size: 1775 1214 1057 1359 1280 5415 Fragments arranged by size: 5415 1775 1359 1280 1214 1057 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 9908 276 Size: 53 83 186 936 8374 2468 Fragments arranged by size: 8374 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI Bsu36I EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI