################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## AatII AclI AcsI AflII AflIII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp700I AspEI AviII BbvCI BciVI BclI BfrI BglII BsaBI BsiWI BsmI Bsp1407I BspLU11I BsrBI BsrGI Bst1107I BstBI BstZ17I Bsu36I CpoI DraI DrdI Eam1105I Eco47III Eco81I EcoRI EcoT22I FbaI FspI HindIII HpaI MamI MfeI MluI MseI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI ScaI SfuI SgrAI SnaBI SpeI SspBI SspI SwaI Tru9I Tsp509I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 2001 GGTACC G^GTACC AccI 190 GTCTAC GT^[AC][GT]AC AccIII 237 TCCGGA T^CCGGA Alw44I 1993 GTGCAC G^TGCAC ApaLI 1993 GTGCAC G^TGCAC Asp718I 2001 GGTACC G^GTACC AspI 493 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 744 CCTAGG C^CTAGG BalI 2568 TGGCCA TGG^CCA BbrPI 2558 CACGTG CAC^GTG BbsI 2386 GTCTTC GAAGAC(2/6) BcgI 1583 GCAAGAAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 744 CCTAGG C^CTAGG BpuAI 2386 GTCTTC GAAGAC(2/6) BsaAI 2558 CACGTG [CT]AC^GT[GA] BseAI 237 TCCGGA T^CCGGA BspDI 2803 ATCGAT AT^CGAT BspEI 237 TCCGGA T^CCGGA BspHI 2018 TCATGA T^CATGA BsrDI 2156 GCAATG GCAATG(2/0) BstEII 291 GGTGACC G^GT[ACGT]ACC BstPI 291 GGTGACC G^GT[ACGT]ACC ClaI 2803 ATCGAT AT^CGAT EcoO65I 291 GGTGACC G^GT[ACGT]ACC EcoRV 841 GATATC GAT^ATC FseI 1290 GGCCGGCC GGCCGG^CC HincII 2494 GTTGAC GT[CT]^[GA]AC HindII 2494 GTTGAC GT[CT]^[GA]AC KpnI 2001 GGTACC GGTAC^C MluNI 2568 TGGCCA TGG^CCA MroI 237 TCCGGA T^CCGGA MscI 2568 TGGCCA TGG^CCA NcoI 1911 CCATGG C^CATGG PflFI 493 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 2558 CACGTG CAC^GTG PmlI 2558 CACGTG CAC^GTG RcaI 2018 TCATGA T^CATGA SapI 1878 GAAGAGC GCTCTTC(1/4) SfiI 1260 GGCCGCGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 2297 GCATGC GCATG^C StuI 531 AGGCCT AGG^CCT TfiI 1061 GAATC G^A[AT]TC Tth111I 493 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### ApaI 69 GGGCCC GGGCC^C 2809 GGGCCC BsaI 1507 GAGACC GGTCTC(1/5) 2638 GAGACC BsaWI 237 TCCGGA [AT]^CCGG[AT] 1706 ACCGGA BssSI 1633 CTCGTG CACGAG(-5/-1) 2564 CTCGTG BstAPI 1896 GCACCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2646 GCAGGCCCTGC EarI 64 GAAGAG CTCTTC(1/4) 1878 GAAGAG Ksp632I 64 GAAGAG CTCTTC(1/4) 1878 GAAGAG MaeIII 292 GTGAC ^GT[ACGT]AC 581 GTGAC MslI 1906 CACCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2656 CACGACCGTG NspI 368 ACATGC [GA]CATG^[CT] 2297 GCATGC PpuMI 21 GGGTCCC [GA]G^G[AT]CC[CT] 53 AGGACCT SacI 2398 GAGCTC GAGCT^C 2482 GAGCTC SexAI 1997 ACCAGGT A^CC[AT]GGT 2207 ACCTGGT SmlI 1762 CTCAAG C^T[CT][GA]AG 2401 CTCAAG Tsp45I 292 GTGAC ^GT[GC]AC 581 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BamHI BanI BanII BbeI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssHII BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EagI EclXI Eco52I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI KspI MaeI MaeII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV PflMI PleI PstI PvuII RsaI SacII Sau3AI Sau96I SbfI ScrFI SfaNI SfcI SfoI SmaI Sse8387I StyI TaqI TseI TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI