Restriction map for
pFN21AE2629
(Circular) (Six-base) MAPSORT of: pFN21AE2629.seq Check: 2234 from: 1 to: 7555
pFN21AE2629 7555 bp
With 182 enzymes: SgfI *
June 10, 2016 17:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3019 3214 4034 4620 3019
Size: 195 820 586 5954
Fragments arranged by size:
5954 820 586 195
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5365 278
Size: 53 83 186 936 3829 2468
Fragments arranged by size:
3829 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2196 4898 1937
Size: 259 2702 4594
Fragments arranged by size:
4594 2702 259
Acc65I G'GTAC_C
Cuts at: 4006 4876 4006
Size: 870 6685
AclI AA'CG_TT
Cuts at: 1563 5682 6055 1563
Size: 4119 373 3063
Fragments arranged by size:
4119 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5618 7002 7270 1632
Size: 3986 1384 268 1917
Fragments arranged by size:
3986 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7358
AflIII A'CryG_T
Cuts at: 1203 6454 7431 1203
Size: 5251 977 1327
Fragments arranged by size:
5251 1327 977
AgeI A'CCGG_T
Cuts at: 6386 6386
Size: 7555
AhdI GACnn_n'nnGTC
Cuts at: 6284 6284
Size: 7555
AleI CACnn'nnGTG
Cuts at: 1166 4665 1166
Size: 3499 4056
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2348 4184 1184
Size: 1164 1836 4555
Fragments arranged by size:
4555 1836 1164
AlwNI CAG_nnn'CTG
Cuts at: 2662 2877 3562 4612 4621 4657 4801 5298 6870
Size: 215 685 1050 9 36 144 497 1572
Cuts at: 6870 2662
Size: 3347
Fragments arranged by size:
3347 1572 1050 685 497 215 144 36
9
ApaI G_GGCC'C
Cuts at: 2078 4818 2078
Size: 2740 4815
ApaLI G'TGCA_C
Cuts at: 3998 5612 6768 3998
Size: 1614 1156 4785
Fragments arranged by size:
4785 1614 1156
ApoI r'AATT_y
Cuts at: 1493 4864 5090 5242 7351 1493
Size: 3371 226 152 2109 1697
Fragments arranged by size:
3371 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6109 160
Size: 872 5077 1606
Fragments arranged by size:
5077 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7555
AvaI C'yCGr_G
Cuts at: 1870 1943 2030 2165 2181 2365 2420 4110 4880
Size: 73 87 135 16 184 55 1690 770
Cuts at: 4880 1870
Size: 4545
Fragments arranged by size:
4545 1690 770 184 135 87 73 55
16
AvrII C'CTAG_G
Cuts at: 2749 2749
Size: 7555
BamHI G'GATC_C
Cuts at: 2017 2159 4479 4885 5252 2017
Size: 142 2320 406 367 4320
Fragments arranged by size:
4320 2320 406 367 142
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2130 2266 2410 3156 3324
Size: 325 217 627 343 136 144 746 168
Cuts at: 3324 3499 4006 4193 4318 4705 4876 6331 618
Size: 175 507 187 125 387 171 1455 1842
Fragments arranged by size:
1842 1455 746 627 507 387 343 325
217 187 175 171 168 144 136 125
BanII G_rGCy'C
Cuts at: 1391 2054 2078 2093 2318 2366 3627 3760 4407
Size: 663 24 15 225 48 1261 133 647
Cuts at: 4407 4491 4728 4818 4874 1391
Size: 84 237 90 56 4072
Fragments arranged by size:
4072 1261 663 647 237 225 133 90
84 56 48 24 15
BbeI G_GCGC'C
Cuts at: 2134 3328 3503 2134
Size: 1194 175 6186
Fragments arranged by size:
6186 1194 175
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4384 928
Size: 971 2485 4099
Fragments arranged by size:
4099 2485 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3577 3611 5746 5780 1903
Size: 34 50 34 1556 34 2135 34 3678
Fragments arranged by size:
3678 2135 1556 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5447 6657 5447
Size: 1210 6345
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7555
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2049 2722 3272
Size: 107 122 71 631 605 377 673 550
Cuts at: 3272 3284 3488 4312 4785 6166 136
Size: 12 204 824 473 1381 1525
Fragments arranged by size:
1525 1381 824 673 631 605 550 473
377 204 122 107 71 12
BglII A'GATC_T
Cuts at: 7550 7550
Size: 7555
BlpI GC'TnA_GC
Cuts at: 2616 3110 3876 4972 2616
Size: 494 766 1096 5199
Fragments arranged by size:
5199 1096 766 494
Bme1580I G_kGCm'C
Cuts at: 1790 2078 2271 3957 4002 4196 4818 5616 6772
Size: 288 193 1686 45 194 622 798 1156
Cuts at: 6772 1790
Size: 2573
Fragments arranged by size:
2573 1686 1156 798 622 288 194 193
45
BmgBI CAC'GTC
Cuts at: 1318 2140 4207 1318
Size: 822 2067 4666
Fragments arranged by size:
4666 2067 822
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2057 3193 3386
Size: 416 53 83 399 24 640 1136 193
Cuts at: 3386 3664 3802 5286 6244 442
Size: 278 138 1484 958 1753
Fragments arranged by size:
1753 1484 1136 958 640 416 399 278
193 138 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7555
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2084 2366 2654 2981 2990 3203 3311 3422
Size: 845 282 288 327 9 213 108 111
Cuts at: 3422 3446 3464 3611 3989 4052 4505 4712 6215
Size: 24 18 147 378 63 453 207 1503
Cuts at: 6215 1239
Size: 2579
Fragments arranged by size:
2579 1503 845 453 378 327 288 282
213 207 147 111 108 63 24 18
9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3752 4391 5680 6545 6843 3752
Size: 639 1289 865 298 4464
Fragments arranged by size:
4464 1289 865 639 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3506 4637 6218 882
Size: 617 2007 1131 1581 2219
Fragments arranged by size:
2219 2007 1581 1131 617
BsaAI yAC'GTr
Cuts at: 493 1204 4565 5346 493
Size: 711 3361 781 2702
Fragments arranged by size:
3361 2702 781 711
BsaBI GATnn'nnATC
Cuts at: 7549 7549
Size: 7555
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2131 2905 3325 3500
Size: 53 83 186 936 598 774 420 175
Cuts at: 3500 3582 4587 5362 5744 6379 275
Size: 82 1005 775 382 635 1451
Fragments arranged by size:
1451 1005 936 775 774 635 598 420
382 186 175 83 82 53
BsaWI w'CCGG_w
Cuts at: 2242 3711 5988 6386 6660 6807 7218 2242
Size: 1469 2277 398 274 147 411 2579
Fragments arranged by size:
2579 2277 1469 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2987 3017 3149 3179 3437
Size: 30 165 30 1584 30 132 30 258
Cuts at: 3437 3467 1178
Size: 30 5266
Fragments arranged by size:
5266 1584 258 165 132 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2987 2990 2993 3230 3737 4709 4736 1186
Size: 1801 3 3 237 507 972 27 4005
Fragments arranged by size:
4005 1801 972 507 237 27 3 3
BseYI C'CCAG_C
Cuts at: 2321 2667 2926 3015 3469 3856 3894 4150 4155
Size: 346 259 89 454 387 38 256 5
Cuts at: 4155 4421 4605 4754 4783 4817 6758 7254 2321
Size: 266 184 149 29 34 1941 496 2622
Fragments arranged by size:
2622 1941 496 454 387 346 266 259
256 184 149 89 38 34 29 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2894 2952 3617 3884 4068 4643 1163
Size: 573 1158 58 665 267 184 575 4075
Fragments arranged by size:
4075 1158 665 575 573 267 184 58
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3261 3375 3483 3530 4666 5005
Size: 275 186 1260 114 108 47 1136 339
Cuts at: 5005 5766 6794 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1260 1136 1028 761 339 275 186
114 108 47
BsiHKAI G_wGCw'C
Cuts at: 3711 3813 4002 4095 4203 4407 4491 4570 5616
Size: 102 189 93 108 204 84 79 1046
Cuts at: 5616 5701 6772 3711
Size: 85 1071 4494
Fragments arranged by size:
4494 1071 1046 204 189 108 102 93
85 84 79
BsmI GAATG_Cn'
Cuts at: 5066 5159 5066
Size: 93 7462
BsmBI CGTCTCn'nnnn_
Cuts at: 2786 4283 4778 2786
Size: 1497 495 5563
Fragments arranged by size:
5563 1497 495
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2078 2093 2271 2318 2366 3627
Size: 399 264 24 15 178 47 48 1261
Cuts at: 3627 3711 3760 3813 3957 4002 4095 4196 4203
Size: 84 49 53 144 45 93 101 7
Cuts at: 4203 4407 4491 4570 4728 4818 4874 5616 5701
Size: 204 84 79 158 90 56 742 85
Cuts at: 5701 6772 1391
Size: 1071 2174
Fragments arranged by size:
2174 1261 1071 742 399 264 204 178
158 144 101 93 90 85 84 84
79 56 53 49 48 47 45 24
15 7
BspEI T'CCGG_A
Cuts at: 2242 7218 2242
Size: 4976 2579
BspHI T'CATG_A
Cuts at: 4023 5444 7174 4023
Size: 1421 1730 4404
Fragments arranged by size:
4404 1730 1421
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2603 2845 3019 3214 4034 4476 4512 4620
Size: 1759 242 174 195 820 442 36 108
Cuts at: 4620 4910 844
Size: 290 3489
Fragments arranged by size:
3489 1759 820 442 290 242 195 174
108 36
BsrBI CCG'CTC
Cuts at: 1394 1823 5442 1394
Size: 429 3619 3507
Fragments arranged by size:
3619 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4168 6050 6224 65
Size: 1183 2920 1882 174 1396
Fragments arranged by size:
2920 1882 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2640 3296 3374 3475 6199 6386 1700
Size: 110 830 656 78 101 2724 187 2869
Fragments arranged by size:
2869 2724 830 656 187 110 101 78
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7555
BssHII G'CGCG_C
Cuts at: 1925 3438 3533 3611 1925
Size: 1513 95 78 5869
Fragments arranged by size:
5869 1513 95 78
BssSI C'ACGA_G
Cuts at: 3638 4569 5615 6627 3638
Size: 931 1046 1012 4566
Fragments arranged by size:
4566 1046 1012 931
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3907 4657 794
Size: 3113 750 3692
Fragments arranged by size:
3692 3113 750
BstBI TT'CG_AA
Cuts at: 6359 6359
Size: 7555
BstEII G'GTnAC_C
Cuts at: 1184 2296 1184
Size: 1112 6443
BstXI CCAn_nnnn'nTGG
Cuts at: 2062 2675 3871 4315 2062
Size: 613 1196 444 5302
Fragments arranged by size:
5302 1196 613 444
BstYI r'GATC_y
Cuts at: 1972 2017 2159 4479 4885 5252 5637 5654 7106
Size: 45 142 2320 406 367 385 17 1452
Cuts at: 7106 7192 7204 7550 1972
Size: 86 12 346 1977
Fragments arranged by size:
2320 1977 1452 406 385 367 346 142
86 45 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2257 2915 3136 3464 3916 4666 513
Size: 552 1192 658 221 328 452 750 3402
Fragments arranged by size:
3402 1192 750 658 552 452 328 221
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6816
Fragments arranged by size:
6816 683 56
BtsI GCAGTG_nn'
Cuts at: 2940 3104 4056 4741 5071 5865 5885 2940
Size: 164 952 685 330 794 20 4610
Fragments arranged by size:
4610 952 794 685 330 164 20
ClaI AT'CG_AT
Cuts at: 1554 4809 1554
Size: 3255 4300
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7520
DraI TTT'AAA
Cuts at: 4859 4859
Size: 7555
DraIII CAC_nnn'GTG
Cuts at: 3095 3640 4207 3095
Size: 545 567 6443
Fragments arranged by size:
6443 567 545
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6562 809
Size: 507 5246 1802
Fragments arranged by size:
5246 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2260 2554 3057
Size: 54 1425 50 275 18 430 294 503
Cuts at: 3057 3258 3273 3372 3480 4573 5002 5891 7520
Size: 201 15 99 108 1093 429 889 1629
Cuts at: 7520 8
Size: 43
Fragments arranged by size:
1629 1425 1093 889 503 430 429 294
275 201 108 99 54 50 43 18
15
EagI C'GGCC_G
Cuts at: 1537 1812 3258 3372 3480 5002 1537
Size: 275 1446 114 108 1522 4090
Fragments arranged by size:
4090 1522 1446 275 114 108
EarI CTCTTCn'nnn_
Cuts at: 1353 2064 3878 5485 1353
Size: 711 1814 1607 3423
Fragments arranged by size:
3423 1814 1607 711
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2306 4766 6142 6516 6662 387
Size: 1064 138 717 2460 1376 374 146 1280
Fragments arranged by size:
2460 1376 1280 1064 717 374 146 138
EcoICRI GAG'CTC
Cuts at: 4405 4489 4405
Size: 84 7471
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2366 2654 2981 2990 3203 3311
Size: 393 452 282 288 327 9 213 108
Cuts at: 3311 3422 3446 3464 3611 3989 4052 4505 4712
Size: 111 24 18 147 378 63 453 207
Cuts at: 4712 5618 6215 7002 7270 1239
Size: 906 597 787 268 1524
Fragments arranged by size:
1524 906 787 597 453 452 393 378
327 288 282 268 213 207 147 111
108 63 24 18 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2993 3212 3545 4793 2993
Size: 219 333 1248 5755
Fragments arranged by size:
5755 1248 333 219
EcoO109I rG'GnC_Cy
Cuts at: 2027 2059 2074 2075 2479 2549 2836 4618 4654
Size: 32 15 1 404 70 287 1782 36
Cuts at: 4654 4699 4798 2027
Size: 45 99 4784
Fragments arranged by size:
4784 1782 404 287 99 70 45 36
32 15 1
EcoRI G'AATT_C
Cuts at: 4864 5242 4864
Size: 378 7177
EcoRV GAT'ATC
Cuts at: 2848 2848
Size: 7555
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2255 2255
Size: 7555
FseI GG_CCGG'CC
Cuts at: 3300 3300
Size: 7555
FspI TGC'GCA
Cuts at: 6061 6061
Size: 7555
HaeII r_GCGC'y
Cuts at: 1678 2134 2884 2957 3194 3328 3503 3576 6702
Size: 456 750 73 237 134 175 73 3126
Cuts at: 6702 1678
Size: 2531
Fragments arranged by size:
3126 2531 750 456 237 175 134 73
73
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 6196 6228 6270 6302 1373
Size: 32 135 32 4624 32 42 32 2626
Fragments arranged by size:
4624 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4501 4899 5145 669
Size: 988 281 2563 398 246 3079
Fragments arranged by size:
3079 2563 988 398 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7555
HpaI GTT'AAC
Cuts at: 5145 5145
Size: 7555
KasI G'GCGC_C
Cuts at: 2130 3324 3499 2130
Size: 1194 175 6186
Fragments arranged by size:
6186 1194 175
KpnI G_GTAC'C
Cuts at: 4010 4880 4010
Size: 870 6685
MfeI C'AATT_G
Cuts at: 5154 5154
Size: 7555
MluI A'CGCG_T
Cuts at: 7431 7431
Size: 7555
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3065 4278 6669 6853 1305
Size: 1760 1213 2391 184 2007
Fragments arranged by size:
2391 2007 1760 1213 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4575 10
Size: 54 1425 343 2743 2990
Fragments arranged by size:
2990 2743 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3915 4665 5513 5872 6031 518
Size: 648 2749 750 848 359 159 2042
Fragments arranged by size:
2749 2042 848 750 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2259 2917 3019 3138 3371 3466 3505 3559
Size: 653 658 102 119 233 95 39 54
Cuts at: 3559 3631 3860 3898 4359 4971 5648 6796 7041
Size: 72 229 38 461 612 677 1148 245
Cuts at: 7041 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 658 653 612 461 245
233 229 119 102 95 72 54 39
38
NaeI GCC'GGC
Cuts at: 3298 3376 3477 3298
Size: 78 101 7376
Fragments arranged by size:
7376 101 78
NarI GG'CG_CC
Cuts at: 2131 3325 3500 2131
Size: 1194 175 6186
Fragments arranged by size:
6186 1194 175
NcoI C'CATG_G
Cuts at: 513 1065 3916 513
Size: 552 2851 4152
Fragments arranged by size:
4152 2851 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7555
NgoMIV G'CCGG_C
Cuts at: 3296 3374 3475 3296
Size: 78 101 7376
Fragments arranged by size:
7376 101 78
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7555
NotI GC'GGCC_GC
Cuts at: 5002 5002
Size: 7555
NspI r_CATG'y
Cuts at: 1132 1229 2377 4306 4913 6458 1132
Size: 97 1148 1929 607 1545 2229
Fragments arranged by size:
2229 1929 1545 1148 607 97
PasI CC'CwG_GG
Cuts at: 1348 2079 2474 2839 1348
Size: 731 395 365 6064
Fragments arranged by size:
6064 731 395 365
PciI A'CATG_T
Cuts at: 6454 6454
Size: 7555
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2140 4145 4582 4801 1108
Size: 1032 2005 437 219 3862
Fragments arranged by size:
3862 2005 1032 437 219
PfoI T'CCnGG_A
Cuts at: 2490 4100 4482 2490
Size: 1610 382 5563
Fragments arranged by size:
5563 1610 382
PmeI GTTT'AAAC
Cuts at: 4859 4859
Size: 7555
PmlI CAC'GTG
Cuts at: 4565 4565
Size: 7555
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5624 5656 7137 7169 5624
Size: 32 1481 32 6010
Fragments arranged by size:
6010 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2027 2059 2027
Size: 32 7523
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7555
PsiI TTA'TAA
Cuts at: 5125 5125
Size: 7555
PspOMI G'GGCC_C
Cuts at: 2074 4814 2074
Size: 2740 4815
PstI C_TGCA'G
Cuts at: 830 2217 2229 2856 3851 4617 4653 4736 4907
Size: 1387 12 627 995 766 36 83 171
Cuts at: 4907 830
Size: 3478
Fragments arranged by size:
3478 1387 995 766 627 171 83 36
12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7555
PvuII CAG'CTG
Cuts at: 3019 3559 3631 3860 3898 3019
Size: 540 72 229 38 6676
Fragments arranged by size:
6676 540 229 72 38
SacI G_AGCT'C
Cuts at: 4407 4491 4407
Size: 84 7471
SacII CC_GC'GG
Cuts at: 2260 2918 3139 3467 2260
Size: 658 221 328 6348
Fragments arranged by size:
6348 658 328 221
SalI G'TCGA_C
Cuts at: 1936 4897 1936
Size: 2961 4594
SanDI GG'GwC_CC
Cuts at: 2027 2027
Size: 7555
SapI GCTCTTCn'nnn_
Cuts at: 3878 3878
Size: 7555
SbfI CC_TGCA'GG
Cuts at: 2856 4617 4653 4907 2856
Size: 1761 36 254 5504
Fragments arranged by size:
5504 1761 254 36
ScaI AGT'ACT
Cuts at: 5803 7515 5803
Size: 1712 5843
SexAI A'CCwGG_T
Cuts at: 4002 4212 4002
Size: 210 7345
SfcI C'TryA_G
Cuts at: 826 1045 2203 2213 2225 2852 3847 4613 4649
Size: 219 1158 10 12 627 995 766 36
Cuts at: 4649 4732 4903 6038 6719 6910 826
Size: 83 171 1135 681 191 1471
Fragments arranged by size:
1471 1158 1135 995 766 681 627 219
191 171 83 36 12 10
SfiI GGCCn_nnn'nGGCC
Cuts at: 3272 3272
Size: 7555
SfoI GGC'GCC
Cuts at: 2132 3326 3501 2132
Size: 1194 175 6186
Fragments arranged by size:
6186 1194 175
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7555
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7555
SmaI CCC'GGG
Cuts at: 1872 2183 2367 2422 4882 1872
Size: 311 184 55 2460 4545
Fragments arranged by size:
4545 2460 311 184 55
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3767 4406 5659 6560 6822 820
Size: 197 926 1824 639 1253 901 262 1553
Fragments arranged by size:
1824 1553 1253 926 901 639 262 197
SnaBI TAC'GTA
Cuts at: 493 5346 493
Size: 4853 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7555
SphI G_CATG'C
Cuts at: 1132 4306 4913 1132
Size: 3174 607 3774
Fragments arranged by size:
3774 3174 607
SrfI GCCC'GGGC
Cuts at: 2183 2183
Size: 7555
SspI AAT'ATT
Cuts at: 5 52 5479 5
Size: 47 5427 2081
Fragments arranged by size:
5427 2081 47
StuI AGG'CCT
Cuts at: 1517 2538 1517
Size: 1021 6534
StyI C'CwwG_G
Cuts at: 513 1065 2055 2454 2749 3916 4994 513
Size: 552 990 399 295 1167 1078 3074
Fragments arranged by size:
3074 1167 1078 990 552 399 295
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2831 2918 3908 4043 4152 4445 5582
Size: 1255 1251 87 990 135 109 293 1137
Cuts at: 5582 5599 5752 5937 6382 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1251 1137 990 445 293 185
153 135 109 87 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5801 7513 96
Size: 274 80 33 51 1443 3824 1712 138
Fragments arranged by size:
3824 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2266 2298 3079 3111 3149 3181 3437 3469 3650
Size: 32 781 32 38 32 256 32 181
Cuts at: 3650 3682 7515 7547 2266
Size: 32 3833 32 2274
Fragments arranged by size:
3833 2274 781 256 181 38 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2501 1316
Size: 1185 6370
XbaI T'CTAG_A
Cuts at: 4891 4891
Size: 7555
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2527 4142 4174 2011
Size: 516 1615 32 5392
Fragments arranged by size:
5392 1615 516 32
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7555
XmaI C'CCGG_G
Cuts at: 1870 2181 2365 2420 4880 1870
Size: 311 184 55 2460 4545
Fragments arranged by size:
4545 2460 311 184 55
XmnI GAAnn'nnTTC
Cuts at: 5684 5684
Size: 7555
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5363 276
Size: 53 83 186 936 3829 2468
Fragments arranged by size:
3829 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BstZ17I Bsu36I FspAI NruI NsiI PacI PspXI
PsrI RsrII SwaI