(Linear) (Six-base) MAPSORT of: KIEE2629.seq Check: 7195 from: 1 to: 2850 KIEE2629.orf With 182 enzymes: SgfI * June 10, 2016 16:09 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1014 1209 2029 2615 2850 Size: 1014 195 820 586 235 Fragments arranged by size: 1014 820 586 235 195 AccI GT'mk_AC Cuts at: 0 191 2850 Size: 191 2659 Acc65I G'GTAC_C Cuts at: 0 2001 2850 Size: 2001 849 AleI CACnn'nnGTG Cuts at: 0 2660 2850 Size: 2660 190 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 343 2179 2850 Size: 343 1836 671 Fragments arranged by size: 1836 671 343 AlwNI CAG_nnn'CTG Cuts at: 0 657 872 1557 2607 2616 2652 2796 2850 Size: 657 215 685 1050 9 36 144 54 Fragments arranged by size: 1050 685 657 215 144 54 36 9 ApaI G_GGCC'C Cuts at: 0 73 2813 2850 Size: 73 2740 37 Fragments arranged by size: 2740 73 37 ApaLI G'TGCA_C Cuts at: 0 1993 2850 Size: 1993 857 AvaI C'yCGr_G Cuts at: 0 25 160 176 360 415 2105 2850 Size: 25 135 16 184 55 1690 745 Fragments arranged by size: 1690 745 184 135 55 25 16 AvrII C'CTAG_G Cuts at: 0 744 2850 Size: 744 2106 BamHI G'GATC_C Cuts at: 0 12 154 2474 2850 Size: 12 142 2320 376 Fragments arranged by size: 2320 376 142 12 BanI G'GyrC_C Cuts at: 0 125 261 405 1151 1319 1494 2001 2188 Size: 125 136 144 746 168 175 507 187 Cuts at: 2188 2313 2700 2850 Size: 125 387 150 Fragments arranged by size: 746 507 387 187 175 168 150 144 136 125 125 BanII G_rGCy'C Cuts at: 0 49 73 88 313 361 1622 1755 2402 Size: 49 24 15 225 48 1261 133 647 Cuts at: 2402 2486 2723 2813 2850 Size: 84 237 90 37 Fragments arranged by size: 1261 647 237 225 133 90 84 49 48 37 24 15 BbeI G_GCGC'C Cuts at: 0 129 1323 1498 2850 Size: 129 1194 175 1352 Fragments arranged by size: 1352 1194 175 129 BbsI GAAGACnn'nnnn_ Cuts at: 0 2379 2850 Size: 2379 471 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1572 1606 2850 Size: 1572 34 1244 Fragments arranged by size: 1572 1244 34 BglI GCCn_nnn'nGGC Cuts at: 0 44 717 1267 1279 1483 2307 2780 2850 Size: 44 673 550 12 204 824 473 70 Fragments arranged by size: 824 673 550 473 204 70 44 12 BlpI GC'TnA_GC Cuts at: 0 611 1105 1871 2850 Size: 611 494 766 979 Fragments arranged by size: 979 766 611 494 Bme1580I G_kGCm'C Cuts at: 0 73 266 1952 1997 2191 2813 2850 Size: 73 193 1686 45 194 622 37 Fragments arranged by size: 1686 622 194 193 73 45 37 BmgBI CAC'GTC Cuts at: 0 135 2202 2850 Size: 135 2067 648 Fragments arranged by size: 2067 648 135 BmrI ACTGGGnnnn_n' Cuts at: 0 52 1188 1381 1659 1797 2850 Size: 52 1136 193 278 138 1053 Fragments arranged by size: 1136 1053 278 193 138 52 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 79 361 649 976 985 1198 1306 1417 Size: 79 282 288 327 9 213 108 111 Cuts at: 1417 1441 1459 1606 1984 2047 2500 2707 2850 Size: 24 18 147 378 63 453 207 143 Fragments arranged by size: 453 378 327 288 282 213 207 147 143 111 108 79 63 24 18 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1747 2386 2850 Size: 1747 639 464 Fragments arranged by size: 1747 639 464 BsaI GGTCTCn'nnnn_ Cuts at: 0 1501 2632 2850 Size: 1501 1131 218 Fragments arranged by size: 1501 1131 218 BsaAI yAC'GTr Cuts at: 0 2560 2850 Size: 2560 290 BsaHI Gr'CG_yC Cuts at: 0 126 900 1320 1495 1577 2582 2850 Size: 126 774 420 175 82 1005 268 Fragments arranged by size: 1005 774 420 268 175 126 82 BsaWI w'CCGG_w Cuts at: 0 237 1706 2850 Size: 237 1469 1144 Fragments arranged by size: 1469 1144 237 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 982 1012 1144 1174 1432 1462 2850 Size: 982 30 132 30 258 30 1388 Fragments arranged by size: 1388 982 258 132 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 982 985 988 1225 1732 2704 2731 2850 Size: 982 3 3 237 507 972 27 119 Fragments arranged by size: 982 972 507 237 119 27 3 3 BseYI C'CCAG_C Cuts at: 0 316 662 921 1010 1464 1851 1889 2145 Size: 316 346 259 89 454 387 38 256 Cuts at: 2145 2150 2416 2600 2749 2778 2812 2850 Size: 5 266 184 149 29 34 38 Fragments arranged by size: 454 387 346 316 266 259 256 184 149 89 38 38 34 29 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 889 947 1612 1879 2063 2638 2850 Size: 889 58 665 267 184 575 212 Fragments arranged by size: 889 665 575 267 212 184 58 BsiEI CG_ry'CG Cuts at: 0 1256 1370 1478 1525 2661 2850 Size: 1256 114 108 47 1136 189 Fragments arranged by size: 1256 1136 189 114 108 47 BsiHKAI G_wGCw'C Cuts at: 0 1706 1808 1997 2090 2198 2402 2486 2565 Size: 1706 102 189 93 108 204 84 79 Cuts at: 2565 2850 Size: 285 Fragments arranged by size: 1706 285 204 189 108 102 93 84 79 BsmBI CGTCTCn'nnnn_ Cuts at: 0 781 2278 2773 2850 Size: 781 1497 495 77 Fragments arranged by size: 1497 781 495 77 Bsp1286I G_dGCh'C Cuts at: 0 49 73 88 266 313 361 1622 1706 Size: 49 24 15 178 47 48 1261 84 Cuts at: 1706 1755 1808 1952 1997 2090 2191 2198 2402 Size: 49 53 144 45 93 101 7 204 Cuts at: 2402 2486 2565 2723 2813 2850 Size: 84 79 158 90 37 Fragments arranged by size: 1261 204 178 158 144 101 93 90 84 84 79 53 49 49 48 47 45 37 24 15 7 BspEI T'CCGG_A Cuts at: 0 237 2850 Size: 237 2613 BspHI T'CATG_A Cuts at: 0 2018 2850 Size: 2018 832 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 598 840 1014 1209 2029 2471 2507 2615 Size: 598 242 174 195 820 442 36 108 Cuts at: 2615 2850 Size: 235 Fragments arranged by size: 820 598 442 242 235 195 174 108 36 BsrDI GCAATG_nn' Cuts at: 0 2163 2850 Size: 2163 687 BsrFI r'CCGG_y Cuts at: 0 635 1291 1369 1470 2850 Size: 635 656 78 101 1380 Fragments arranged by size: 1380 656 635 101 78 BssHII G'CGCG_C Cuts at: 0 1433 1528 1606 2850 Size: 1433 95 78 1244 Fragments arranged by size: 1433 1244 95 78 BssSI C'ACGA_G Cuts at: 0 1633 2564 2850 Size: 1633 931 286 Fragments arranged by size: 1633 931 286 BstAPI GCAn_nnn'nTGC Cuts at: 0 1902 2652 2850 Size: 1902 750 198 Fragments arranged by size: 1902 750 198 BstEII G'GTnAC_C Cuts at: 0 291 2850 Size: 291 2559 BstXI CCAn_nnnn'nTGG Cuts at: 0 57 670 1866 2310 2850 Size: 57 613 1196 444 540 Fragments arranged by size: 1196 613 540 444 57 BstYI r'GATC_y Cuts at: 0 12 154 2474 2850 Size: 12 142 2320 376 Fragments arranged by size: 2320 376 142 12 BtgI C'CryG_G Cuts at: 0 252 910 1131 1459 1911 2661 2850 Size: 252 658 221 328 452 750 189 Fragments arranged by size: 750 658 452 328 252 221 189 BtsI GCAGTG_nn' Cuts at: 0 935 1099 2051 2736 2850 Size: 935 164 952 685 114 Fragments arranged by size: 952 935 685 164 114 ClaI AT'CG_AT Cuts at: 0 2804 2850 Size: 2804 46 DraIII CAC_nnn'GTG Cuts at: 0 1090 1635 2202 2850 Size: 1090 545 567 648 Fragments arranged by size: 1090 648 567 545 EaeI y'GGCC_r Cuts at: 0 255 549 1052 1253 1268 1367 1475 2568 Size: 255 294 503 201 15 99 108 1093 Cuts at: 2568 2850 Size: 282 Fragments arranged by size: 1093 503 294 282 255 201 108 99 15 EagI C'GGCC_G Cuts at: 0 1253 1367 1475 2850 Size: 1253 114 108 1375 Fragments arranged by size: 1375 1253 114 108 EarI CTCTTCn'nnn_ Cuts at: 0 59 1873 2850 Size: 59 1814 977 Fragments arranged by size: 1814 977 59 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 301 2761 2850 Size: 301 2460 89 Fragments arranged by size: 2460 301 89 EcoICRI GAG'CTC Cuts at: 0 2400 2484 2850 Size: 2400 84 366 Fragments arranged by size: 2400 366 84 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 79 361 649 976 985 1198 1306 1417 Size: 79 282 288 327 9 213 108 111 Cuts at: 1417 1441 1459 1606 1984 2047 2500 2707 2850 Size: 24 18 147 378 63 453 207 143 Fragments arranged by size: 453 378 327 288 282 213 207 147 143 111 108 79 63 24 18 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 988 1207 1540 2788 2850 Size: 988 219 333 1248 62 Fragments arranged by size: 1248 988 333 219 62 EcoO109I rG'GnC_Cy Cuts at: 0 22 54 69 70 474 544 831 2613 Size: 22 32 15 1 404 70 287 1782 Cuts at: 2613 2649 2694 2793 2850 Size: 36 45 99 57 Fragments arranged by size: 1782 404 287 99 70 57 45 36 32 22 15 1 EcoRV GAT'ATC Cuts at: 0 843 2850 Size: 843 2007 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 250 2850 Size: 250 2600 FseI GG_CCGG'CC Cuts at: 0 1295 2850 Size: 1295 1555 HaeII r_GCGC'y Cuts at: 0 129 879 952 1189 1323 1498 1571 2850 Size: 129 750 73 237 134 175 73 1279 Fragments arranged by size: 1279 750 237 175 134 129 73 73 HincII GTy'rAC Cuts at: 0 2496 2850 Size: 2496 354 KasI G'GCGC_C Cuts at: 0 125 1319 1494 2850 Size: 125 1194 175 1356 Fragments arranged by size: 1356 1194 175 125 KpnI G_GTAC'C Cuts at: 0 2005 2850 Size: 2005 845 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1060 2273 2850 Size: 1060 1213 577 Fragments arranged by size: 1213 1060 577 MscI TGG'CCA Cuts at: 0 2570 2850 Size: 2570 280 MslI CAynn'nnrTG Cuts at: 0 1910 2660 2850 Size: 1910 750 190 Fragments arranged by size: 1910 750 190 MspA1I CmG'CkG Cuts at: 0 254 912 1014 1133 1366 1461 1500 1554 Size: 254 658 102 119 233 95 39 54 Cuts at: 1554 1626 1855 1893 2354 2850 Size: 72 229 38 461 496 Fragments arranged by size: 658 496 461 254 233 229 119 102 95 72 54 39 38 NaeI GCC'GGC Cuts at: 0 1293 1371 1472 2850 Size: 1293 78 101 1378 Fragments arranged by size: 1378 1293 101 78 NarI GG'CG_CC Cuts at: 0 126 1320 1495 2850 Size: 126 1194 175 1355 Fragments arranged by size: 1355 1194 175 126 NcoI C'CATG_G Cuts at: 0 1911 2850 Size: 1911 939 NgoMIV G'CCGG_C Cuts at: 0 1291 1369 1470 2850 Size: 1291 78 101 1380 Fragments arranged by size: 1380 1291 101 78 NspI r_CATG'y Cuts at: 0 372 2301 2850 Size: 372 1929 549 Fragments arranged by size: 1929 549 372 PasI CC'CwG_GG Cuts at: 0 74 469 834 2850 Size: 74 395 365 2016 Fragments arranged by size: 2016 395 365 74 PflMI CCAn_nnn'nTGG Cuts at: 0 135 2140 2577 2796 2850 Size: 135 2005 437 219 54 Fragments arranged by size: 2005 437 219 135 54 PfoI T'CCnGG_A Cuts at: 0 485 2095 2477 2850 Size: 485 1610 382 373 Fragments arranged by size: 1610 485 382 373 PmlI CAC'GTG Cuts at: 0 2560 2850 Size: 2560 290 PpuMI rG'GwC_Cy Cuts at: 0 22 54 2850 Size: 22 32 2796 Fragments arranged by size: 2796 32 22 PspOMI G'GGCC_C Cuts at: 0 69 2809 2850 Size: 69 2740 41 Fragments arranged by size: 2740 69 41 PstI C_TGCA'G Cuts at: 0 212 224 851 1846 2612 2648 2731 2850 Size: 212 12 627 995 766 36 83 119 Fragments arranged by size: 995 766 627 212 119 83 36 12 PvuII CAG'CTG Cuts at: 0 1014 1554 1626 1855 1893 2850 Size: 1014 540 72 229 38 957 Fragments arranged by size: 1014 957 540 229 72 38 SacI G_AGCT'C Cuts at: 0 2402 2486 2850 Size: 2402 84 364 Fragments arranged by size: 2402 364 84 SacII CC_GC'GG Cuts at: 0 255 913 1134 1462 2850 Size: 255 658 221 328 1388 Fragments arranged by size: 1388 658 328 255 221 SanDI GG'GwC_CC Cuts at: 0 22 2850 Size: 22 2828 SapI GCTCTTCn'nnn_ Cuts at: 0 1873 2850 Size: 1873 977 SbfI CC_TGCA'GG Cuts at: 0 851 2612 2648 2850 Size: 851 1761 36 202 Fragments arranged by size: 1761 851 202 36 SexAI A'CCwGG_T Cuts at: 0 1997 2207 2850 Size: 1997 210 643 Fragments arranged by size: 1997 643 210 SfcI C'TryA_G Cuts at: 0 198 208 220 847 1842 2608 2644 2727 Size: 198 10 12 627 995 766 36 83 Cuts at: 2727 2850 Size: 123 Fragments arranged by size: 995 766 627 198 123 83 36 12 10 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1267 2850 Size: 1267 1583 SfoI GGC'GCC Cuts at: 0 127 1321 1496 2850 Size: 127 1194 175 1354 Fragments arranged by size: 1354 1194 175 127 SmaI CCC'GGG Cuts at: 0 178 362 417 2850 Size: 178 184 55 2433 Fragments arranged by size: 2433 184 178 55 SmlI C'TyrA_G Cuts at: 0 1762 2401 2850 Size: 1762 639 449 Fragments arranged by size: 1762 639 449 SphI G_CATG'C Cuts at: 0 2301 2850 Size: 2301 549 SrfI GCCC'GGGC Cuts at: 0 178 2850 Size: 178 2672 StuI AGG'CCT Cuts at: 0 533 2850 Size: 533 2317 StyI C'CwwG_G Cuts at: 0 50 449 744 1911 2850 Size: 50 399 295 1167 939 Fragments arranged by size: 1167 939 399 295 50 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 826 913 1903 2038 2147 2440 2850 Size: 826 87 990 135 109 293 410 Fragments arranged by size: 990 826 410 293 135 109 87 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 261 293 1074 1106 1144 1176 1432 1464 Size: 261 32 781 32 38 32 256 32 Cuts at: 1464 1645 1677 2850 Size: 181 32 1173 Fragments arranged by size: 1173 781 261 256 181 38 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 496 2850 Size: 496 2354 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 522 2137 2169 2850 Size: 522 1615 32 681 Fragments arranged by size: 1615 681 522 32 XmaI C'CCGG_G Cuts at: 0 176 360 415 2850 Size: 176 184 55 2435 Fragments arranged by size: 2435 184 176 55 Enzymes that do cut: AarI AccI Acc65I AleI AlfI AlwNI ApaI ApaLI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BglI BlpI Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI BtgI BtsI ClaI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FseI HaeII HincII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SacI SacII SanDI SapI SbfI SexAI SfcI SfiI SfoI SmaI SmlI SphI SrfI StuI StyI TaqII TstI Tth111I XcmI XmaI Enzymes that do not cut: AatII AclI AcuI AfeI AflII AflIII AgeI AhdI AloI ApoI AscI AseI AsiSI BaeI BbvCI BciVI BclI BfrBI BglII BmtI BplI Bpu10I BsaBI BsiWI BsmI BsrBI BsrGI BstBI BstZ17I Bsu36I BtgZI CspCI DraI DrdI EcoRI FspI FspAI Hin4I HindIII HpaI MfeI MluI NdeI NheI NotI NruI NsiI PacI PciI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI ScaI SgfI SgrAI SnaBI SpeI SspI SwaI TatI XbaI XhoI XmnI ZraI