(Circular) (Six-base) MAPSORT of: pFN21AE2624.seq Check: 5934 from: 1 to: 5485 pFN21AE2624 5485 bp With 182 enzymes: SgfI * May 26, 2016 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2586 2586 Size: 5485 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3295 278 Size: 53 83 186 936 1759 2468 Fragments arranged by size: 2468 1759 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2828 1937 Size: 891 4594 Acc65I G'GTAC_C Cuts at: 2806 2806 Size: 5485 AclI AA'CG_TT Cuts at: 1563 3612 3985 1563 Size: 2049 373 3063 Fragments arranged by size: 3063 2049 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3548 4932 5200 1632 Size: 1916 1384 268 1917 Fragments arranged by size: 1917 1916 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5288 AflIII A'CryG_T Cuts at: 1203 4384 5361 1203 Size: 3181 977 1327 Fragments arranged by size: 3181 1327 977 AgeI A'CCGG_T Cuts at: 4316 4316 Size: 5485 AhdI GACnn_n'nnGTC Cuts at: 4214 4214 Size: 5485 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5485 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5485 AlwNI CAG_nnn'CTG Cuts at: 2341 2614 2695 3228 4800 2341 Size: 273 81 533 1572 3026 Fragments arranged by size: 3026 1572 533 273 81 ApaI G_GGCC'C Cuts at: 2110 2170 2607 2110 Size: 60 437 4988 Fragments arranged by size: 4988 437 60 ApaLI G'TGCA_C Cuts at: 3542 4698 3542 Size: 1156 4329 ApoI r'AATT_y Cuts at: 1493 2567 2794 3020 3172 5281 1493 Size: 1074 227 226 152 2109 1697 Fragments arranged by size: 2109 1697 1074 227 226 152 AscI GG'CGCG_CC Cuts at: 2179 2179 Size: 5485 AseI AT'TA_AT Cuts at: 160 1032 4039 160 Size: 872 3007 1606 Fragments arranged by size: 3007 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5485 AvaI C'yCGr_G Cuts at: 1870 1943 2779 2810 1870 Size: 73 836 31 4545 Fragments arranged by size: 4545 836 73 31 BamHI G'GATC_C Cuts at: 2815 3182 2815 Size: 367 5118 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2040 2233 2246 2296 2410 Size: 325 217 627 253 193 13 50 114 Cuts at: 2410 2806 4261 618 Size: 396 1455 1842 Fragments arranged by size: 1842 1455 627 396 325 253 217 193 114 50 13 BanII G_rGCy'C Cuts at: 1391 2110 2170 2391 2607 2804 1391 Size: 719 60 221 216 197 4072 Fragments arranged by size: 4072 719 221 216 197 60 BbeI G_GCGC'C Cuts at: 2044 2237 2300 2414 2044 Size: 193 63 114 5115 Fragments arranged by size: 5115 193 114 63 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4514 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3676 3710 1903 Size: 34 1739 34 3678 Fragments arranged by size: 3678 1739 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3377 4587 3377 Size: 1210 4275 BclI T'GATC_A Cuts at: 1548 1548 Size: 5485 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2015 4096 136 Size: 107 122 71 631 605 343 2081 1525 Fragments arranged by size: 2081 1525 631 605 343 122 107 71 BglII A'GATC_T Cuts at: 5480 5480 Size: 5485 BlpI GC'TnA_GC Cuts at: 2902 2902 Size: 5485 Bme1580I G_kGCm'C Cuts at: 1790 2110 2170 2607 3546 4702 1790 Size: 320 60 437 939 1156 2573 Fragments arranged by size: 2573 1156 939 437 320 60 BmgBI CAC'GTC Cuts at: 1318 2500 1318 Size: 1182 4303 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3216 4174 442 Size: 416 53 83 399 24 1799 958 1753 Fragments arranged by size: 1799 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5485 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2288 4145 1239 Size: 1049 1857 2579 Fragments arranged by size: 2579 1857 1049 Bpu10I CC'TnA_GC Cuts at: 2744 2744 Size: 5485 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3610 4475 4773 3610 Size: 865 298 4322 Fragments arranged by size: 4322 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2618 4148 882 Size: 617 1119 1530 2219 Fragments arranged by size: 2219 1530 1119 617 BsaAI yAC'GTr Cuts at: 493 1204 3276 493 Size: 711 2072 2702 Fragments arranged by size: 2702 2072 711 BsaBI GATnn'nnATC Cuts at: 5479 5479 Size: 5485 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2041 2234 2273 2297 Size: 53 83 186 936 508 193 39 24 Cuts at: 2297 2411 2421 3292 3674 4309 275 Size: 114 10 871 382 635 1451 Fragments arranged by size: 1451 936 871 635 508 382 193 186 114 83 53 39 24 10 BsaWI w'CCGG_w Cuts at: 3918 4316 4590 4737 5148 3918 Size: 398 274 147 411 4255 Fragments arranged by size: 4255 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5260 Fragments arranged by size: 5260 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2038 2270 2717 1186 Size: 852 232 447 3954 Fragments arranged by size: 3954 852 447 232 BseYI C'CCAG_C Cuts at: 2095 2203 2237 2527 2607 4688 5184 2095 Size: 108 34 290 80 2081 496 2396 Fragments arranged by size: 2396 2081 496 290 108 80 34 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2354 2558 2786 1163 Size: 573 618 204 228 3862 Fragments arranged by size: 3862 618 573 228 204 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2174 2488 2935 3696 4724 1540 Size: 275 186 173 314 447 761 1028 2301 Fragments arranged by size: 2301 1028 761 447 314 275 186 173 BsiHKAI G_wGCw'C Cuts at: 2670 3546 3631 4702 2670 Size: 876 85 1071 3453 Fragments arranged by size: 3453 1071 876 85 BsmI GAATG_Cn' Cuts at: 2617 2996 3089 2617 Size: 379 93 5013 Fragments arranged by size: 5013 379 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2110 2170 2391 2607 2670 2804 3546 Size: 399 320 60 221 216 63 134 742 Cuts at: 3546 3631 4702 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 320 221 216 134 85 63 60 BspEI T'CCGG_A Cuts at: 5148 5148 Size: 5485 BspHI T'CATG_A Cuts at: 3374 5104 3374 Size: 1730 3755 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2466 2586 2698 2840 844 Size: 1622 120 112 142 3489 Fragments arranged by size: 3489 1622 142 120 112 BsrBI CCG'CTC Cuts at: 1394 1823 2635 3372 1394 Size: 429 812 737 3507 Fragments arranged by size: 3507 812 737 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3980 4154 65 Size: 1183 2732 174 1396 Fragments arranged by size: 2732 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2299 2581 4129 4316 1700 Size: 110 489 282 1548 187 2869 Fragments arranged by size: 2869 1548 489 282 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5485 BssHII G'CGCG_C Cuts at: 1925 2112 2179 1925 Size: 187 67 5231 Fragments arranged by size: 5231 187 67 BssSI C'ACGA_G Cuts at: 3545 4557 3545 Size: 1012 4473 BstAPI GCAn_nnn'nTGC Cuts at: 794 2341 2713 794 Size: 1547 372 3566 Fragments arranged by size: 3566 1547 372 BstBI TT'CG_AA Cuts at: 4289 4289 Size: 5485 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5485 BstYI r'GATC_y Cuts at: 1972 2815 3182 3567 3584 5036 5122 5134 5480 Size: 843 367 385 17 1452 86 12 346 Cuts at: 5480 1972 Size: 1977 Fragments arranged by size: 1977 1452 843 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2010 2359 513 Size: 552 939 6 349 3639 Fragments arranged by size: 3639 939 552 349 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4746 Fragments arranged by size: 4746 683 56 BtsI GCAGTG_nn' Cuts at: 3001 3795 3815 3001 Size: 794 20 4671 Fragments arranged by size: 4671 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5485 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5450 DraI TTT'AAA Cuts at: 2789 2789 Size: 5485 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4492 809 Size: 507 3176 1802 Fragments arranged by size: 3176 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2171 2301 Size: 54 1425 50 275 18 177 164 130 Cuts at: 2301 2362 2485 2932 3821 5450 8 Size: 61 123 447 889 1629 43 Fragments arranged by size: 1629 1425 889 447 275 177 164 130 123 61 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2171 2485 2932 1537 Size: 275 359 314 447 4090 Fragments arranged by size: 4090 447 359 314 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2645 3415 1353 Size: 1292 770 3423 Fragments arranged by size: 3423 1292 770 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2171 2786 4072 4446 4592 387 Size: 1064 138 582 615 1286 374 146 1280 Fragments arranged by size: 1286 1280 1064 615 582 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2288 3548 4145 4932 5200 1239 Size: 393 656 1260 597 787 268 1524 Fragments arranged by size: 1524 1260 787 656 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2438 2747 2438 Size: 309 5176 EcoO109I rG'GnC_Cy Cuts at: 2100 2166 2280 2491 2532 2604 2776 2100 Size: 66 114 211 41 72 172 4809 Fragments arranged by size: 4809 211 172 114 72 66 41 EcoRI G'AATT_C Cuts at: 2567 2794 3172 2567 Size: 227 378 4880 Fragments arranged by size: 4880 378 227 FspI TGC'GCA Cuts at: 3991 3991 Size: 5485 HaeII r_GCGC'y Cuts at: 1678 2044 2237 2264 2300 2414 2696 4632 1678 Size: 366 193 27 36 114 282 1936 2531 Fragments arranged by size: 2531 1936 366 282 193 114 36 27 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4126 4158 4200 4232 1373 Size: 32 135 32 2554 32 42 32 2626 Fragments arranged by size: 2626 2554 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2380 2829 3075 669 Size: 988 281 442 449 246 3079 Fragments arranged by size: 3079 988 449 442 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5485 HpaI GTT'AAC Cuts at: 3075 3075 Size: 5485 KasI G'GCGC_C Cuts at: 2040 2233 2296 2410 2040 Size: 193 63 114 5115 Fragments arranged by size: 5115 193 114 63 KpnI G_GTAC'C Cuts at: 2810 2810 Size: 5485 MfeI C'AATT_G Cuts at: 3084 3084 Size: 5485 MluI A'CGCG_T Cuts at: 5361 5361 Size: 5485 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4599 4783 1305 Size: 3294 184 2007 Fragments arranged by size: 3294 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3663 Fragments arranged by size: 3663 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3443 3802 3961 518 Size: 648 2277 359 159 2042 Fragments arranged by size: 2277 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2012 2186 2469 2614 2901 3578 4726 4971 Size: 406 174 283 145 287 677 1148 245 Cuts at: 4971 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 406 287 283 245 174 145 NaeI GCC'GGC Cuts at: 2301 2583 2301 Size: 282 5203 NarI GG'CG_CC Cuts at: 2041 2234 2297 2411 2041 Size: 193 63 114 5115 Fragments arranged by size: 5115 193 114 63 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3994 Fragments arranged by size: 3994 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5485 NgoMIV G'CCGG_C Cuts at: 2299 2581 2299 Size: 282 5203 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5485 NotI GC'GGCC_GC Cuts at: 2932 2932 Size: 5485 NspI r_CATG'y Cuts at: 1132 1229 2843 4388 1132 Size: 97 1614 1545 2229 Fragments arranged by size: 2229 1614 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5485 PciI A'CATG_T Cuts at: 4384 4384 Size: 5485 PflMI CCAn_nnn'nTGG Cuts at: 1108 2761 1108 Size: 1653 3832 PfoI T'CCnGG_A Cuts at: 2121 2493 2121 Size: 372 5113 PmeI GTTT'AAAC Cuts at: 2789 2789 Size: 5485 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3554 3586 5067 5099 3554 Size: 32 1481 32 3940 Fragments arranged by size: 3940 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2100 2280 2491 2100 Size: 180 211 5094 Fragments arranged by size: 5094 211 180 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5485 PsiI TTA'TAA Cuts at: 3055 3055 Size: 5485 PspOMI G'GGCC_C Cuts at: 2106 2166 2603 2106 Size: 60 437 4988 Fragments arranged by size: 4988 437 60 PstI C_TGCA'G Cuts at: 830 2144 2463 2837 830 Size: 1314 319 374 3478 Fragments arranged by size: 3478 1314 374 319 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5485 PvuII CAG'CTG Cuts at: 2614 2614 Size: 5485 SacII CC_GC'GG Cuts at: 2013 2013 Size: 5485 SalI G'TCGA_C Cuts at: 1936 2827 1936 Size: 891 4594 SanDI GG'GwC_CC Cuts at: 2280 2280 Size: 5485 SapI GCTCTTCn'nnn_ Cuts at: 2645 2645 Size: 5485 SbfI CC_TGCA'GG Cuts at: 2144 2837 2144 Size: 693 4792 ScaI AGT'ACT Cuts at: 3733 5445 3733 Size: 1712 3773 SfcI C'TryA_G Cuts at: 826 1045 2140 2459 2833 3968 4649 4840 826 Size: 219 1095 319 374 1135 681 191 1471 Fragments arranged by size: 1471 1135 1095 681 374 319 219 191 SfoI GGC'GCC Cuts at: 2042 2235 2298 2412 2042 Size: 193 63 114 5115 Fragments arranged by size: 5115 193 114 63 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5485 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5485 SmaI CCC'GGG Cuts at: 1872 2812 1872 Size: 940 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3589 4490 4752 820 Size: 197 926 1646 901 262 1553 Fragments arranged by size: 1646 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3276 493 Size: 2783 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5485 SphI G_CATG'C Cuts at: 1132 2843 1132 Size: 1711 3774 SspI AAT'ATT Cuts at: 5 52 3409 5 Size: 47 3357 2081 Fragments arranged by size: 3357 2081 47 StuI AGG'CCT Cuts at: 1517 2138 1517 Size: 621 4864 StyI C'CwwG_G Cuts at: 513 1065 2004 2924 513 Size: 552 939 920 3074 Fragments arranged by size: 3074 939 920 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2489 2512 3512 3529 3682 3867 4312 Size: 1255 909 23 1000 17 153 185 445 Cuts at: 4312 325 Size: 1498 Fragments arranged by size: 1498 1255 1000 909 445 185 153 23 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2652 3731 5443 Size: 274 80 33 51 1443 675 1079 1712 Cuts at: 5443 96 Size: 138 Fragments arranged by size: 1712 1443 1079 675 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5445 5477 5445 Size: 32 5453 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5485 XbaI T'CTAG_A Cuts at: 2821 2821 Size: 5485 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5485 XmaI C'CCGG_G Cuts at: 1870 2810 1870 Size: 940 4545 XmnI GAAnn'nnTTC Cuts at: 3614 3614 Size: 5485 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3293 276 Size: 53 83 186 936 1759 2468 Fragments arranged by size: 2468 1759 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SexAI SfiI SrfI SwaI XcmI