(Circular) (Six-base) MAPSORT of: pFN21AE2582.seq Check: 6590 from: 1 to: 7573 pFN21AE2582 7573 bp With 182 enzymes: SgfI * June 20, 2019 11:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5383 278 Size: 53 83 186 936 3847 2468 Fragments arranged by size: 3847 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2460 3470 4916 1937 Size: 523 1010 1446 4594 Fragments arranged by size: 4594 1446 1010 523 Acc65I G'GTAC_C Cuts at: 3093 4894 3093 Size: 1801 5772 AclI AA'CG_TT Cuts at: 1563 5700 6073 1563 Size: 4137 373 3063 Fragments arranged by size: 4137 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2590 2774 2783 3365 3416 5636 7020 7288 Size: 958 184 9 582 51 2220 1384 268 Cuts at: 7288 1632 Size: 1917 Fragments arranged by size: 2220 1917 1384 958 582 268 184 51 9 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7376 AflIII A'CryG_T Cuts at: 1203 6472 7449 1203 Size: 5269 977 1327 Fragments arranged by size: 5269 1327 977 AgeI A'CCGG_T Cuts at: 6404 6404 Size: 7573 AhdI GACnn_n'nnGTC Cuts at: 6302 6302 Size: 7573 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7573 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4511 4545 4841 1184 Size: 3327 34 296 3916 Fragments arranged by size: 3916 3327 296 34 AlwNI CAG_nnn'CTG Cuts at: 2308 3850 4124 4133 4406 5316 6888 2308 Size: 1542 274 9 273 910 1572 2993 Fragments arranged by size: 2993 1572 1542 910 274 273 9 ApaI G_GGCC'C Cuts at: 2886 2886 Size: 7573 ApaLI G'TGCA_C Cuts at: 5630 6786 5630 Size: 1156 6417 ApoI r'AATT_y Cuts at: 1493 3438 3659 4882 5108 5260 7369 1493 Size: 1945 221 1223 226 152 2109 1697 Fragments arranged by size: 2109 1945 1697 1223 226 221 152 AseI AT'TA_AT Cuts at: 160 1032 6127 160 Size: 872 5095 1606 Fragments arranged by size: 5095 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7573 AvaI C'yCGr_G Cuts at: 1870 1943 2848 2922 4835 4898 1870 Size: 73 905 74 1913 63 4545 Fragments arranged by size: 4545 1913 905 74 73 63 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2924 2957 2924 Size: 33 7540 BamHI G'GATC_C Cuts at: 4032 4903 5270 4032 Size: 871 367 6335 Fragments arranged by size: 6335 871 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2333 2672 3093 4894 6349 Size: 325 217 627 546 339 421 1801 1455 Cuts at: 6349 618 Size: 1842 Fragments arranged by size: 1842 1801 1455 627 546 421 339 325 217 BanII G_rGCy'C Cuts at: 1391 2886 3328 4190 4659 4892 1391 Size: 1495 442 862 469 233 4072 Fragments arranged by size: 4072 1495 862 469 442 233 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2109 2451 3538 3624 4355 4478 928 Size: 971 210 342 1087 86 731 123 4023 Fragments arranged by size: 4023 1087 971 731 342 210 123 86 BbvCI CC'TCA_GC Cuts at: 2547 3060 4452 4558 2547 Size: 513 1392 106 5562 Fragments arranged by size: 5562 1392 513 106 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4313 4347 5764 5798 1903 Size: 34 2376 34 1417 34 3678 Fragments arranged by size: 3678 2376 1417 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5465 6675 5465 Size: 1210 6363 BclI T'GATC_A Cuts at: 1548 1548 Size: 7573 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4046 6184 136 Size: 107 122 71 631 605 2374 2138 1525 Fragments arranged by size: 2374 2138 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7568 7568 Size: 7573 BlpI GC'TnA_GC Cuts at: 2786 4700 4990 2786 Size: 1914 290 5369 Fragments arranged by size: 5369 1914 290 Bme1580I G_kGCm'C Cuts at: 1790 2675 2886 4603 5634 6790 1790 Size: 885 211 1717 1031 1156 2573 Fragments arranged by size: 2573 1717 1156 1031 885 211 BmgBI CAC'GTC Cuts at: 1318 2776 1318 Size: 1458 6115 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2073 2966 4416 Size: 416 53 83 399 24 656 893 1450 Cuts at: 4416 5304 6262 442 Size: 888 958 1753 Fragments arranged by size: 1753 1450 958 893 888 656 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7573 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4543 4543 Size: 7573 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2834 3935 4064 4100 4214 4345 4787 6233 Size: 1595 1101 129 36 114 131 442 1446 Cuts at: 6233 1239 Size: 2579 Fragments arranged by size: 2579 1595 1446 1101 442 131 129 114 36 Bpu10I CC'TnA_GC Cuts at: 2547 3060 4452 4558 2547 Size: 513 1392 106 5562 Fragments arranged by size: 5562 1392 513 106 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2424 5698 6563 6861 2424 Size: 3274 865 298 3136 Fragments arranged by size: 3274 3136 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3546 6236 882 Size: 617 2047 2690 2219 Fragments arranged by size: 2690 2219 2047 617 BsaAI yAC'GTr Cuts at: 493 1204 5364 493 Size: 711 4160 2702 Fragments arranged by size: 4160 2702 711 BsaBI GATnn'nnATC Cuts at: 7567 7567 Size: 7573 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5380 5762 6397 275 Size: 53 83 186 936 3847 382 635 1451 Fragments arranged by size: 3847 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2532 6006 6404 6678 6825 7236 2532 Size: 3474 398 274 147 411 2869 Fragments arranged by size: 3474 2869 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2213 2243 3203 3233 4058 Size: 30 165 30 810 30 960 30 825 Cuts at: 4058 4088 1178 Size: 30 4663 Fragments arranged by size: 4663 960 825 810 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2594 3048 3368 3809 4040 4097 4229 4232 Size: 1408 454 320 441 231 57 132 3 Cuts at: 4232 4235 4348 1186 Size: 3 113 4411 Fragments arranged by size: 4411 1408 454 441 320 231 132 113 57 3 3 BseYI C'CCAG_C Cuts at: 2895 3281 3327 3855 3942 3994 4501 4667 4685 Size: 386 46 528 87 52 507 166 18 Cuts at: 4685 4738 6776 7272 2895 Size: 53 2038 496 3196 Fragments arranged by size: 3196 2038 528 507 496 386 166 87 53 52 46 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2409 3194 4250 4811 1163 Size: 573 673 785 1056 561 3925 Fragments arranged by size: 3925 1056 785 673 573 561 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4696 5023 5784 6812 1540 Size: 275 186 2695 327 761 1028 2301 Fragments arranged by size: 2695 2301 1028 761 327 275 186 BsiHKAI G_wGCw'C Cuts at: 2994 3256 4091 4190 4515 4659 4706 5634 5719 Size: 262 835 99 325 144 47 928 85 Cuts at: 5719 6790 2994 Size: 1071 3777 Fragments arranged by size: 3777 1071 928 835 325 262 144 99 85 47 BsmI GAATG_Cn' Cuts at: 4117 5084 5177 4117 Size: 967 93 6513 Fragments arranged by size: 6513 967 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2675 2886 2994 3256 3328 4091 4190 Size: 399 885 211 108 262 72 763 99 Cuts at: 4190 4515 4603 4659 4706 4892 5634 5719 6790 Size: 325 88 56 47 186 742 85 1071 Cuts at: 6790 1391 Size: 2174 Fragments arranged by size: 2174 1071 885 763 742 399 325 262 211 186 108 99 88 85 72 56 47 BspEI T'CCGG_A Cuts at: 7236 7236 Size: 7573 BspHI T'CATG_A Cuts at: 3538 5462 7192 3538 Size: 1924 1730 3919 Fragments arranged by size: 3919 1924 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2894 4928 844 Size: 2050 2034 3489 Fragments arranged by size: 3489 2050 2034 BsrBI CCG'CTC Cuts at: 1394 1823 2781 3895 4030 4380 5460 1394 Size: 429 958 1114 135 350 1080 3507 Fragments arranged by size: 3507 1114 1080 958 429 350 135 BsrDI GCAATG_nn' Cuts at: 65 1248 6068 6242 65 Size: 1183 4820 174 1396 Fragments arranged by size: 4820 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4695 6217 6404 1700 Size: 110 2885 1522 187 2869 Fragments arranged by size: 2885 2869 1522 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7573 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7573 BssSI C'ACGA_G Cuts at: 3032 5633 6645 3032 Size: 2601 1012 3960 Fragments arranged by size: 3960 2601 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4124 794 Size: 3330 4243 BstBI TT'CG_AA Cuts at: 6377 6377 Size: 7573 BstEII G'GTnAC_C Cuts at: 1184 3008 1184 Size: 1824 5749 BstXI CCAn_nnnn'nTGG Cuts at: 3020 3293 3020 Size: 273 7300 BstYI r'GATC_y Cuts at: 1972 2209 2247 3741 4032 4903 5270 5655 5672 Size: 237 38 1494 291 871 367 385 17 Cuts at: 5672 7124 7210 7222 7568 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1494 1452 871 385 367 346 291 237 86 38 17 12 Bsu36I CC'TnA_GG Cuts at: 2243 2243 Size: 7573 BtgI C'CryG_G Cuts at: 513 1065 2424 2650 4690 4783 513 Size: 552 1359 226 2040 93 3303 Fragments arranged by size: 3303 2040 1359 552 226 93 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6834 Fragments arranged by size: 6834 683 56 BtsI GCAGTG_nn' Cuts at: 3206 3708 4122 4535 4603 5089 5883 5903 3206 Size: 502 414 413 68 486 794 20 4876 Fragments arranged by size: 4876 794 502 486 414 413 68 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7573 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7538 DraI TTT'AAA Cuts at: 4877 4877 Size: 7573 DraIII CAC_nnn'GTG Cuts at: 2503 2503 Size: 7573 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6580 809 Size: 507 5264 1802 Fragments arranged by size: 5264 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2361 3081 3961 Size: 54 1425 50 275 18 531 720 880 Cuts at: 3961 4693 4743 4780 5020 5909 7538 8 Size: 732 50 37 240 889 1629 43 Fragments arranged by size: 1629 1425 889 880 732 720 531 275 240 54 50 50 43 37 18 EagI C'GGCC_G Cuts at: 1537 1812 4693 5020 1537 Size: 275 2881 327 4090 Fragments arranged by size: 4090 2881 327 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3196 3786 4461 4664 5503 1353 Size: 1843 590 675 203 839 3423 Fragments arranged by size: 3423 1843 839 675 590 203 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3184 6160 6534 6680 387 Size: 1064 138 1595 2976 374 146 1280 Fragments arranged by size: 2976 1595 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4188 4657 4188 Size: 469 7104 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2590 2774 2783 2834 3365 3416 3935 Size: 393 958 184 9 51 531 51 519 Cuts at: 3935 4064 4100 4214 4345 4787 5636 6233 7020 Size: 129 36 114 131 442 849 597 787 Cuts at: 7020 7288 1239 Size: 268 1524 Fragments arranged by size: 1524 958 849 787 597 531 519 442 393 268 184 131 129 114 51 51 36 9 EcoNI CCTnn'n_nnAGG Cuts at: 3749 3749 Size: 7573 EcoO109I rG'GnC_Cy Cuts at: 2055 2882 2883 2927 3847 4315 2055 Size: 827 1 44 920 468 5313 Fragments arranged by size: 5313 920 827 468 44 1 EcoRI G'AATT_C Cuts at: 3659 4882 5260 3659 Size: 1223 378 5972 Fragments arranged by size: 5972 1223 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2231 2231 Size: 7573 FspI TGC'GCA Cuts at: 6079 6079 Size: 7573 HaeII r_GCGC'y Cuts at: 1678 2753 6720 1678 Size: 1075 3967 2531 Fragments arranged by size: 3967 2531 1075 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4402 4434 4699 4731 4797 Size: 32 135 32 2830 32 265 32 66 Cuts at: 4797 4829 6214 6246 6288 6320 1373 Size: 32 1385 32 42 32 2626 Fragments arranged by size: 2830 2626 1385 265 135 66 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4917 5163 669 Size: 988 281 2979 246 3079 Fragments arranged by size: 3079 2979 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7573 HpaI GTT'AAC Cuts at: 5163 5163 Size: 7573 KpnI G_GTAC'C Cuts at: 3097 4898 3097 Size: 1801 5772 MfeI C'AATT_G Cuts at: 5172 5172 Size: 7573 MluI A'CGCG_T Cuts at: 7449 7449 Size: 7573 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4120 6687 6871 1305 Size: 2815 2567 184 2007 Fragments arranged by size: 2815 2567 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2363 3963 4782 10 Size: 54 1425 343 531 1600 819 2801 Fragments arranged by size: 2801 1600 1425 819 531 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3957 4521 5531 5890 6049 518 Size: 648 2791 564 1010 359 159 2042 Fragments arranged by size: 2791 2042 1010 648 564 359 159 MspA1I CmG'CkG Cuts at: 1606 2288 2305 2806 3201 3859 4133 4447 4692 Size: 682 17 501 395 658 274 314 245 Cuts at: 4692 4742 4989 5666 6814 7059 1606 Size: 50 247 677 1148 245 2120 Fragments arranged by size: 2120 1148 682 677 658 501 395 314 274 247 245 245 50 17 NaeI GCC'GGC Cuts at: 4697 4697 Size: 7573 NcoI C'CATG_G Cuts at: 513 1065 2650 4783 513 Size: 552 1585 2133 3303 Fragments arranged by size: 3303 2133 1585 552 NdeI CA'TA_TG Cuts at: 387 3817 387 Size: 3430 4143 NgoMIV G'CCGG_C Cuts at: 4695 4695 Size: 7573 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7573 NotI GC'GGCC_GC Cuts at: 5020 5020 Size: 7573 NspI r_CATG'y Cuts at: 1132 1229 3873 3979 4526 4931 6476 1132 Size: 97 2644 106 547 405 1545 2229 Fragments arranged by size: 2644 2229 1545 547 405 106 97 PasI CC'CwG_GG Cuts at: 1348 3020 3247 1348 Size: 1672 227 5674 Fragments arranged by size: 5674 1672 227 PciI A'CATG_T Cuts at: 6472 6472 Size: 7573 PflMI CCAn_nnn'nTGG Cuts at: 1108 3037 3334 1108 Size: 1929 297 5347 Fragments arranged by size: 5347 1929 297 PfoI T'CCnGG_A Cuts at: 2313 3744 4002 2313 Size: 1431 258 5884 Fragments arranged by size: 5884 1431 258 PmeI GTTT'AAAC Cuts at: 4877 4877 Size: 7573 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5642 5674 7155 7187 5642 Size: 32 1481 32 6028 Fragments arranged by size: 6028 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2055 2927 4315 2055 Size: 872 1388 5313 Fragments arranged by size: 5313 1388 872 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7573 PsiI TTA'TAA Cuts at: 5143 5143 Size: 7573 PspOMI G'GGCC_C Cuts at: 2882 2882 Size: 7573 PstI C_TGCA'G Cuts at: 830 2905 3631 3843 3864 4129 4323 4925 830 Size: 2075 726 212 21 265 194 602 3478 Fragments arranged by size: 3478 2075 726 602 265 212 194 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7573 PvuII CAG'CTG Cuts at: 2288 2806 3859 4133 4447 2288 Size: 518 1053 274 314 5414 Fragments arranged by size: 5414 1053 518 314 274 SacI G_AGCT'C Cuts at: 4190 4659 4190 Size: 469 7104 SacII CC_GC'GG Cuts at: 4693 4693 Size: 7573 SalI G'TCGA_C Cuts at: 1936 4915 1936 Size: 2979 4594 SanDI GG'GwC_CC Cuts at: 2055 2055 Size: 7573 SapI GCTCTTCn'nnn_ Cuts at: 4664 4664 Size: 7573 SbfI CC_TGCA'GG Cuts at: 2905 4925 2905 Size: 2020 5553 ScaI AGT'ACT Cuts at: 5821 7533 5821 Size: 1712 5861 SexAI A'CCwGG_T Cuts at: 2499 2499 Size: 7573 SfcI C'TryA_G Cuts at: 826 1045 2901 3627 3839 3860 4125 4319 4921 Size: 219 1856 726 212 21 265 194 602 Cuts at: 4921 6056 6737 6928 826 Size: 1135 681 191 1471 Fragments arranged by size: 1856 1471 1135 726 681 602 265 219 212 194 191 21 SfiI GGCCn_nnn'nGGCC Cuts at: 4046 4046 Size: 7573 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7573 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7573 SmaI CCC'GGG Cuts at: 1872 4900 1872 Size: 3028 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2439 5677 6578 6840 820 Size: 197 926 496 3238 901 262 1553 Fragments arranged by size: 3238 1553 926 901 496 262 197 SnaBI TAC'GTA Cuts at: 493 5364 493 Size: 4871 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7573 SphI G_CATG'C Cuts at: 1132 4526 4931 1132 Size: 3394 405 3774 Fragments arranged by size: 3774 3394 405 SspI AAT'ATT Cuts at: 5 52 5497 5 Size: 47 5445 2081 Fragments arranged by size: 5445 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7573 StyI C'CwwG_G Cuts at: 513 1065 2650 4783 5012 513 Size: 552 1585 2133 229 3074 Fragments arranged by size: 3074 2133 1585 552 229 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3239 4567 4752 5600 5617 5770 5955 Size: 1255 1659 1328 185 848 17 153 185 Cuts at: 5955 6400 325 Size: 445 1498 Fragments arranged by size: 1659 1498 1328 1255 848 445 185 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5819 7531 96 Size: 274 80 33 51 1443 3842 1712 138 Fragments arranged by size: 3842 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3203 3235 3700 3732 7533 7565 3203 Size: 32 465 32 3801 32 3211 Fragments arranged by size: 3801 3211 465 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7573 XbaI T'CTAG_A Cuts at: 2098 4909 2098 Size: 2811 4762 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3950 4166 4444 3950 Size: 216 278 7079 Fragments arranged by size: 7079 278 216 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7573 XmaI C'CCGG_G Cuts at: 1870 4898 1870 Size: 3028 4545 XmnI GAAnn'nnTTC Cuts at: 5702 5702 Size: 7573 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5381 276 Size: 53 83 186 936 3847 2468 Fragments arranged by size: 3847 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SfoI SrfI SwaI