(Circular) (Six-base) MAPSORT of: pFN21AE2578.seq Check: 4974 from: 1 to: 8209 pFN21AE2578 8209 bp With 182 enzymes: SgfI * June 25, 2015 16:09 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2099 2365 3003 4354 4377 4439 4738 5276 2099 Size: 266 638 1351 23 62 299 538 5032 Fragments arranged by size: 5032 1351 638 538 299 266 62 23 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6019 278 Size: 53 83 186 936 4483 2468 Fragments arranged by size: 4483 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2742 3360 4757 5552 1937 Size: 805 618 1397 795 4594 Fragments arranged by size: 4594 1397 805 795 618 Acc65I G'GTAC_C Cuts at: 5530 5530 Size: 8209 AclI AA'CG_TT Cuts at: 1563 6336 6709 1563 Size: 4773 373 3063 Fragments arranged by size: 4773 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 6272 7656 7924 1632 Size: 4640 1384 268 1917 Fragments arranged by size: 4640 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8012 AflIII A'CryG_T Cuts at: 1203 3621 4284 7108 8085 1203 Size: 2418 663 2824 977 1327 Fragments arranged by size: 2824 2418 1327 977 663 AgeI A'CCGG_T Cuts at: 7040 7040 Size: 8209 AhdI GACnn_n'nnGTC Cuts at: 6938 6938 Size: 8209 AleI CACnn'nnGTG Cuts at: 1166 2495 3342 1166 Size: 1329 847 6033 Fragments arranged by size: 6033 1329 847 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2889 3202 4136 1184 Size: 1705 313 934 5257 Fragments arranged by size: 5257 1705 934 313 AlwNI CAG_nnn'CTG Cuts at: 3202 3574 5952 7524 3202 Size: 372 2378 1572 3887 Fragments arranged by size: 3887 2378 1572 372 ApaI G_GGCC'C Cuts at: 2183 5276 5501 2183 Size: 3093 225 4891 Fragments arranged by size: 4891 3093 225 ApaLI G'TGCA_C Cuts at: 3187 3443 6266 7422 3187 Size: 256 2823 1156 3974 Fragments arranged by size: 3974 2823 1156 256 ApoI r'AATT_y Cuts at: 1493 4336 5518 5744 5896 8005 1493 Size: 2843 1182 226 152 2109 1697 Fragments arranged by size: 2843 2109 1697 1182 226 152 AseI AT'TA_AT Cuts at: 160 1032 6763 160 Size: 872 5731 1606 Fragments arranged by size: 5731 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8209 AvaI C'yCGr_G Cuts at: 1870 1943 2103 3321 3555 3720 4890 5534 1870 Size: 73 160 1218 234 165 1170 644 4545 Fragments arranged by size: 4545 1218 1170 644 234 165 160 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2274 2307 3554 3587 2274 Size: 33 1247 33 6896 Fragments arranged by size: 6896 1247 33 33 BamHI G'GATC_C Cuts at: 4000 5539 5906 4000 Size: 1539 367 6303 Fragments arranged by size: 6303 1539 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2091 2190 2323 2335 2361 Size: 325 217 627 304 99 133 12 26 Cuts at: 2361 2724 2873 3665 3842 4418 5530 6985 618 Size: 363 149 792 177 576 1112 1455 1842 Fragments arranged by size: 1842 1455 1112 792 627 576 363 325 304 217 177 149 133 99 26 12 BanII G_rGCy'C Cuts at: 1391 2183 2586 3225 4545 5276 5302 5501 5528 Size: 792 403 639 1320 731 26 199 27 Cuts at: 5528 1391 Size: 4072 Fragments arranged by size: 4072 1320 792 731 639 403 199 27 26 BbeI G_GCGC'C Cuts at: 2095 2327 2365 3669 2095 Size: 232 38 1304 6635 Fragments arranged by size: 6635 1304 232 38 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3078 3099 4033 4483 928 Size: 971 1179 21 934 450 4654 Fragments arranged by size: 4654 1179 971 934 450 21 BbvCI CC'TCA_GC Cuts at: 2316 2532 2789 2316 Size: 216 257 7736 Fragments arranged by size: 7736 257 216 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2990 3024 3701 3735 6400 6434 1903 Size: 34 1053 34 677 34 2665 34 3678 Fragments arranged by size: 3678 2665 1053 677 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2275 6101 7311 2275 Size: 3826 1210 3173 Fragments arranged by size: 3826 3173 1210 BclI T'GATC_A Cuts at: 1548 3916 1548 Size: 2368 5841 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2546 3537 3609 Size: 107 122 71 631 605 874 991 72 Cuts at: 3609 3701 3810 6820 136 Size: 92 109 3010 1525 Fragments arranged by size: 3010 1525 991 874 631 605 122 109 107 92 72 71 BglII A'GATC_T Cuts at: 3111 3276 8204 3111 Size: 165 4928 3116 Fragments arranged by size: 4928 3116 165 BlpI GC'TnA_GC Cuts at: 4743 5626 4743 Size: 883 7326 Bme1580I G_kGCm'C Cuts at: 1790 2183 2195 2340 2729 3191 3447 3845 4033 Size: 393 12 145 389 462 256 398 188 Cuts at: 4033 4225 4421 5276 5501 6270 7426 1790 Size: 192 196 855 225 769 1156 2573 Fragments arranged by size: 2573 1156 855 769 462 398 393 389 256 225 196 192 188 145 12 BmgBI CAC'GTC Cuts at: 1318 4870 1318 Size: 3552 4657 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3178 5270 5940 Size: 416 53 83 399 24 1761 2092 670 Cuts at: 5940 6898 442 Size: 958 1753 Fragments arranged by size: 2092 1761 1753 958 670 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8209 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3394 3394 Size: 8209 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2432 5009 5453 6869 1239 Size: 1193 2577 444 1416 2579 Fragments arranged by size: 2579 2577 1416 1193 444 Bpu10I CC'TnA_GC Cuts at: 2316 2532 2789 3887 2316 Size: 216 257 1098 6638 Fragments arranged by size: 6638 1098 257 216 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3614 3995 6334 7199 7497 3614 Size: 381 2339 865 298 4326 Fragments arranged by size: 4326 2339 865 381 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2957 4634 6872 882 Size: 617 1458 1677 2238 2219 Fragments arranged by size: 2238 2219 1677 1458 617 BsaAI yAC'GTr Cuts at: 493 1204 2644 3389 6000 493 Size: 711 1440 745 2611 2702 Fragments arranged by size: 2702 2611 1440 745 711 BsaBI GATnn'nnATC Cuts at: 3275 8203 3275 Size: 4928 3281 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2092 2208 2324 2362 Size: 53 83 186 936 559 116 116 38 Cuts at: 2362 3453 3666 4614 6016 6398 7033 275 Size: 1091 213 948 1402 382 635 1451 Fragments arranged by size: 1451 1402 1091 948 936 635 559 382 213 186 116 116 83 53 38 BsaWI w'CCGG_w Cuts at: 2217 4836 6642 7040 7314 7461 7872 2217 Size: 2619 1806 398 274 147 411 2554 Fragments arranged by size: 2619 2554 1806 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2405 2435 4005 4035 1178 Size: 30 165 30 1002 30 1570 30 5352 Fragments arranged by size: 5352 1570 1002 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2750 3701 4823 4832 5081 1186 Size: 1564 951 1122 9 249 4314 Fragments arranged by size: 4314 1564 1122 951 249 9 BseYI C'CCAG_C Cuts at: 2162 2248 2650 3045 3753 4567 4576 5501 7412 Size: 86 402 395 708 814 9 925 1911 Cuts at: 7412 7908 2162 Size: 496 2463 Fragments arranged by size: 2463 1911 925 814 708 496 402 395 86 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2104 2393 2690 2881 2902 4341 4481 Size: 573 368 289 297 191 21 1439 140 Cuts at: 4481 1163 Size: 4891 Fragments arranged by size: 4891 1439 573 368 297 289 191 140 21 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5377 5659 6420 7448 1540 Size: 275 186 3376 282 761 1028 2301 Fragments arranged by size: 3376 2301 1028 761 282 275 186 BsiHKAI G_wGCw'C Cuts at: 3191 3225 3447 5232 6270 6355 7426 3191 Size: 34 222 1785 1038 85 1071 3974 Fragments arranged by size: 3974 1785 1071 1038 222 85 34 BsmI GAATG_Cn' Cuts at: 5720 5813 5720 Size: 93 8116 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2183 2195 2340 2586 2729 3191 3225 Size: 399 393 12 145 246 143 462 34 Cuts at: 3225 3447 3845 4033 4225 4421 4545 5232 5276 Size: 222 398 188 192 196 124 687 44 Cuts at: 5276 5302 5501 5528 6270 6355 7426 1391 Size: 26 199 27 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 687 462 399 398 393 246 222 199 196 192 188 145 143 124 85 44 34 27 26 12 BspEI T'CCGG_A Cuts at: 2217 7872 2217 Size: 5655 2554 BspHI T'CATG_A Cuts at: 3207 6098 7828 3207 Size: 2891 1730 3588 Fragments arranged by size: 3588 2891 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2099 2365 2662 3003 4354 4377 4439 4738 Size: 1255 266 297 341 1351 23 62 299 Cuts at: 4738 5276 5564 844 Size: 538 288 3489 Fragments arranged by size: 3489 1351 1255 538 341 299 297 288 266 62 23 BsrBI CCG'CTC Cuts at: 1394 1823 3022 4215 5071 5163 6096 1394 Size: 429 1199 1193 856 92 933 3507 Fragments arranged by size: 3507 1199 1193 933 856 429 92 BsrDI GCAATG_nn' Cuts at: 65 1248 2077 4275 4909 4956 6704 6878 65 Size: 1183 829 2198 634 47 1748 174 1396 Fragments arranged by size: 2198 1748 1396 1183 829 634 174 47 BsrFI r'CCGG_y Cuts at: 1700 1810 2601 3942 6853 7040 1700 Size: 110 791 1341 2911 187 2869 Fragments arranged by size: 2911 2869 1341 791 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8209 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8209 BssSI C'ACGA_G Cuts at: 6269 7281 6269 Size: 1012 7197 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8209 BstBI TT'CG_AA Cuts at: 7013 7013 Size: 8209 BstEII G'GTnAC_C Cuts at: 1184 5386 1184 Size: 4202 4007 BstXI CCAn_nnnn'nTGG Cuts at: 2632 3382 3517 3733 4870 2632 Size: 750 135 216 1137 5971 Fragments arranged by size: 5971 1137 750 216 135 BstYI r'GATC_y Cuts at: 1972 3111 3276 3970 4000 4101 5539 5906 6291 Size: 1139 165 694 30 101 1438 367 385 Cuts at: 6291 6308 7760 7846 7858 8204 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1438 1139 694 385 367 346 165 101 86 30 17 12 Bsu36I CC'TnA_GG Cuts at: 3266 3864 4179 5125 3266 Size: 598 315 946 6350 Fragments arranged by size: 6350 946 598 315 BtgI C'CryG_G Cuts at: 513 1065 2095 2842 2968 3057 3604 3732 4618 Size: 552 1030 747 126 89 547 128 886 Cuts at: 4618 5391 513 Size: 773 3331 Fragments arranged by size: 3331 1030 886 773 747 552 547 128 126 89 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2731 5023 487 Size: 683 56 1505 2292 3673 Fragments arranged by size: 3673 2292 1505 683 56 BtsI GCAGTG_nn' Cuts at: 2268 2673 3189 3259 3337 3445 3563 4356 5172 Size: 405 516 70 78 108 118 793 816 Cuts at: 5172 5512 5725 6519 6539 2268 Size: 340 213 794 20 3938 Fragments arranged by size: 3938 816 794 793 516 405 340 213 118 108 78 70 20 ClaI AT'CG_AT Cuts at: 1554 3900 1554 Size: 2346 5863 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4570 4605 529 Size: 35 4006 35 4133 Fragments arranged by size: 4133 4006 35 35 DraI TTT'AAA Cuts at: 5513 5513 Size: 8209 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7216 809 Size: 507 5900 1802 Fragments arranged by size: 5900 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2403 3507 3538 Size: 54 1425 50 275 18 573 1104 31 Cuts at: 3538 3702 4479 4521 5656 6545 8174 8 Size: 164 777 42 1135 889 1629 43 Fragments arranged by size: 1629 1425 1135 1104 889 777 573 275 164 54 50 43 42 31 18 EagI C'GGCC_G Cuts at: 1537 1812 5656 1537 Size: 275 3844 4090 Fragments arranged by size: 4090 3844 275 EarI CTCTTCn'nnn_ Cuts at: 1353 6139 1353 Size: 4786 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2603 3791 6796 7170 7316 387 Size: 1064 138 1014 1188 3005 374 146 1280 Fragments arranged by size: 3005 1280 1188 1064 1014 374 146 138 EcoICRI GAG'CTC Cuts at: 3223 3223 Size: 8209 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2432 5009 5453 6272 6869 7656 7924 Size: 393 800 2577 444 819 597 787 268 Cuts at: 7924 1239 Size: 1524 Fragments arranged by size: 2577 1524 819 800 787 597 444 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 4412 5402 4412 Size: 990 7219 EcoO109I rG'GnC_Cy Cuts at: 2180 4183 4571 5368 5497 5498 2180 Size: 2003 388 797 129 1 4891 Fragments arranged by size: 4891 2003 797 388 129 1 EcoRI G'AATT_C Cuts at: 5518 5896 5518 Size: 378 7831 FspI TGC'GCA Cuts at: 3195 6715 3195 Size: 3520 4689 HaeII r_GCGC'y Cuts at: 1678 2095 2327 2365 2419 2685 2950 3669 4730 Size: 417 232 38 54 266 265 719 1061 Cuts at: 4730 4900 7356 1678 Size: 170 2456 2531 Fragments arranged by size: 2531 2456 1061 719 417 266 265 232 170 54 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3473 3505 3955 3987 5433 Size: 32 135 32 1901 32 450 32 1446 Cuts at: 5433 5465 6850 6882 6924 6956 1373 Size: 32 1385 32 42 32 2626 Fragments arranged by size: 2626 1901 1446 1385 450 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4758 5553 5799 669 Size: 988 281 2820 795 246 3079 Fragments arranged by size: 3079 2820 988 795 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8209 HpaI GTT'AAC Cuts at: 5799 5799 Size: 8209 KasI G'GCGC_C Cuts at: 2091 2323 2361 3665 2091 Size: 232 38 1304 6635 Fragments arranged by size: 6635 1304 232 38 KpnI G_GTAC'C Cuts at: 5534 5534 Size: 8209 MfeI C'AATT_G Cuts at: 5808 5808 Size: 8209 MluI A'CGCG_T Cuts at: 3621 8085 3621 Size: 4464 3745 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 7323 7507 1305 Size: 6018 184 2007 Fragments arranged by size: 6018 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3509 3704 4481 4523 10 Size: 54 1425 343 1677 195 777 42 3696 Fragments arranged by size: 3696 1677 1425 777 343 195 54 42 MslI CAynn'nnrTG Cuts at: 518 1166 2495 2591 2630 2771 3062 3132 3184 Size: 648 1329 96 39 141 291 70 52 Cuts at: 3184 3342 3419 4091 5114 5223 6167 6526 6685 Size: 158 77 672 1023 109 944 359 159 Cuts at: 6685 518 Size: 2042 Fragments arranged by size: 2042 1329 1023 944 672 648 359 291 159 158 141 109 96 77 70 52 39 MspA1I CmG'CkG Cuts at: 1606 2303 2428 2495 2793 2970 3435 3715 3922 Size: 697 125 67 298 177 465 280 207 Cuts at: 3922 4667 5083 5431 5625 6302 7450 7695 1606 Size: 745 416 348 194 677 1148 245 2120 Fragments arranged by size: 2120 1148 745 697 677 465 416 348 298 280 245 207 194 177 125 67 NarI GG'CG_CC Cuts at: 2092 2324 2362 3666 2092 Size: 232 38 1304 6635 Fragments arranged by size: 6635 1304 232 38 NcoI C'CATG_G Cuts at: 513 1065 3057 3732 4618 513 Size: 552 1992 675 886 4104 Fragments arranged by size: 4104 1992 886 675 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8209 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8209 NotI GC'GGCC_GC Cuts at: 5656 5656 Size: 8209 NspI r_CATG'y Cuts at: 1132 1229 2776 3418 4288 4465 4742 5567 7112 Size: 97 1547 642 870 177 277 825 1545 Cuts at: 7112 1132 Size: 2229 Fragments arranged by size: 2229 1547 1545 870 825 642 277 177 97 PasI CC'CwG_GG Cuts at: 1348 2269 1348 Size: 921 7288 PciI A'CATG_T Cuts at: 4284 7108 4284 Size: 2824 5385 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 3605 4624 4885 1108 Size: 902 1595 1019 261 4432 Fragments arranged by size: 4432 1595 1019 902 261 PfoI T'CCnGG_A Cuts at: 2145 3776 3989 2145 Size: 1631 213 6365 Fragments arranged by size: 6365 1631 213 PmeI GTTT'AAAC Cuts at: 5513 5513 Size: 8209 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5057 5089 6278 6310 7791 7823 5057 Size: 32 1189 32 1481 32 5443 Fragments arranged by size: 5443 1481 1189 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4571 5368 4571 Size: 797 7412 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8209 PsiI TTA'TAA Cuts at: 5779 5779 Size: 8209 PspOMI G'GGCC_C Cuts at: 2179 5272 5497 2179 Size: 3093 225 4891 Fragments arranged by size: 4891 3093 225 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3569 3601 3569 Size: 32 8177 PstI C_TGCA'G Cuts at: 830 3000 3570 3714 4374 4436 4672 5561 830 Size: 2170 570 144 660 62 236 889 3478 Fragments arranged by size: 3478 2170 889 660 570 236 144 62 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8209 PvuII CAG'CTG Cuts at: 3922 4667 5083 5431 3922 Size: 745 416 348 6700 Fragments arranged by size: 6700 745 416 348 SacI G_AGCT'C Cuts at: 3225 3225 Size: 8209 SacII CC_GC'GG Cuts at: 2971 2971 Size: 8209 SalI G'TCGA_C Cuts at: 1936 4756 5551 1936 Size: 2820 795 4594 Fragments arranged by size: 4594 2820 795 SanDI GG'GwC_CC Cuts at: 4571 5368 4571 Size: 797 7412 SbfI CC_TGCA'GG Cuts at: 5561 5561 Size: 8209 ScaI AGT'ACT Cuts at: 3986 4448 6457 8169 3986 Size: 462 2009 1712 4026 Fragments arranged by size: 4026 2009 1712 462 SexAI A'CCwGG_T Cuts at: 5403 5403 Size: 8209 SfcI C'TryA_G Cuts at: 826 1045 2996 3566 3710 4370 4432 4668 4787 Size: 219 1951 570 144 660 62 236 119 Cuts at: 4787 4823 5557 6692 7373 7564 826 Size: 36 734 1135 681 191 1471 Fragments arranged by size: 1951 1471 1135 734 681 660 570 236 219 191 144 119 62 36 SfiI GGCCn_nnn'nGGCC Cuts at: 3537 3701 3537 Size: 164 8045 SfoI GGC'GCC Cuts at: 2093 2325 2363 3667 2093 Size: 232 38 1304 6635 Fragments arranged by size: 6635 1304 232 38 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8209 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8209 SmaI CCC'GGG Cuts at: 1872 3323 4892 5536 1872 Size: 1451 1569 644 4545 Fragments arranged by size: 4545 1569 1451 644 SmlI C'TyrA_G Cuts at: 820 1017 1943 3629 3974 6313 7214 7476 820 Size: 197 926 1686 345 2339 901 262 1553 Fragments arranged by size: 2339 1686 1553 926 901 345 262 197 SnaBI TAC'GTA Cuts at: 493 6000 493 Size: 5507 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8209 SphI G_CATG'C Cuts at: 1132 2776 4742 5567 1132 Size: 1644 1966 825 3774 Fragments arranged by size: 3774 1966 1644 825 SrfI GCCC'GGGC Cuts at: 3323 3323 Size: 8209 SspI AAT'ATT Cuts at: 5 52 6133 5 Size: 47 6081 2081 Fragments arranged by size: 6081 2081 47 StuI AGG'CCT Cuts at: 1517 2954 3797 1517 Size: 1437 843 5929 Fragments arranged by size: 5929 1437 843 StyI C'CwwG_G Cuts at: 513 1065 3057 3457 3732 3783 3792 4618 4863 Size: 552 1992 400 275 51 9 826 245 Cuts at: 4863 5418 5648 513 Size: 555 230 3074 Fragments arranged by size: 3074 1992 826 555 552 400 275 245 230 51 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2176 2620 3860 5391 6236 6253 6406 Size: 1255 596 444 1240 1531 845 17 153 Cuts at: 6406 6591 7036 325 Size: 185 445 1498 Fragments arranged by size: 1531 1498 1255 1240 845 596 445 444 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3984 4155 4446 Size: 274 80 33 51 1443 2007 171 291 Cuts at: 4446 6455 8167 96 Size: 2009 1712 138 Fragments arranged by size: 2009 2007 1712 1443 291 274 171 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2612 2644 2911 2943 8169 8201 2612 Size: 32 267 32 5226 32 2620 Fragments arranged by size: 5226 2620 267 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3134 1316 Size: 1818 6391 XbaI T'CTAG_A Cuts at: 5545 5545 Size: 8209 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5200 5255 5200 Size: 55 8154 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8209 XmaI C'CCGG_G Cuts at: 1870 3321 4890 5534 1870 Size: 1451 1569 644 4545 Fragments arranged by size: 4545 1569 1451 644 XmnI GAAnn'nnTTC Cuts at: 2393 6338 2393 Size: 3945 4264 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6017 276 Size: 53 83 186 936 4483 2468 Fragments arranged by size: 4483 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI RsrII SapI SwaI