(Linear) (Six-base) MAPSORT of: KIEE2578.seq Check: 678 from: 1 to: 3504 KIEE2578.orf With 182 enzymes: SgfI * June 25, 2015 11:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 94 360 998 2349 2372 2434 2733 3271 Size: 94 266 638 1351 23 62 299 538 Cuts at: 3271 3504 Size: 233 Fragments arranged by size: 1351 638 538 299 266 233 94 62 23 AccI GT'mk_AC Cuts at: 0 737 1355 2752 3504 Size: 737 618 1397 752 Fragments arranged by size: 1397 752 737 618 AflIII A'CryG_T Cuts at: 0 1616 2279 3504 Size: 1616 663 1225 Fragments arranged by size: 1616 1225 663 AleI CACnn'nnGTG Cuts at: 0 490 1337 3504 Size: 490 847 2167 Fragments arranged by size: 2167 847 490 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 884 1197 2131 3504 Size: 884 313 934 1373 Fragments arranged by size: 1373 934 884 313 AlwNI CAG_nnn'CTG Cuts at: 0 1197 1569 3504 Size: 1197 372 1935 Fragments arranged by size: 1935 1197 372 ApaI G_GGCC'C Cuts at: 0 178 3271 3496 3504 Size: 178 3093 225 8 Fragments arranged by size: 3093 225 178 8 ApaLI G'TGCA_C Cuts at: 0 1182 1438 3504 Size: 1182 256 2066 Fragments arranged by size: 2066 1182 256 ApoI r'AATT_y Cuts at: 0 2331 3504 Size: 2331 1173 AvaI C'yCGr_G Cuts at: 0 98 1316 1550 1715 2885 3504 Size: 98 1218 234 165 1170 619 Fragments arranged by size: 1218 1170 619 234 165 98 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 269 302 1549 1582 3504 Size: 269 33 1247 33 1922 Fragments arranged by size: 1922 1247 269 33 33 BamHI G'GATC_C Cuts at: 0 1995 3504 Size: 1995 1509 BanI G'GyrC_C Cuts at: 0 86 185 318 330 356 719 868 1660 Size: 86 99 133 12 26 363 149 792 Cuts at: 1660 1837 2413 3504 Size: 177 576 1091 Fragments arranged by size: 1091 792 576 363 177 149 133 99 86 26 12 BanII G_rGCy'C Cuts at: 0 178 581 1220 2540 3271 3297 3496 3504 Size: 178 403 639 1320 731 26 199 8 Fragments arranged by size: 1320 731 639 403 199 178 26 8 BbeI G_GCGC'C Cuts at: 0 90 322 360 1664 3504 Size: 90 232 38 1304 1840 Fragments arranged by size: 1840 1304 232 90 38 BbsI GAAGACnn'nnnn_ Cuts at: 0 1073 1094 2028 2478 3504 Size: 1073 21 934 450 1026 Fragments arranged by size: 1073 1026 934 450 21 BbvCI CC'TCA_GC Cuts at: 0 311 527 784 3504 Size: 311 216 257 2720 Fragments arranged by size: 2720 311 257 216 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 985 1019 1696 1730 3504 Size: 985 34 677 34 1774 Fragments arranged by size: 1774 985 677 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 270 3504 Size: 270 3234 BclI T'GATC_A Cuts at: 0 1911 3504 Size: 1911 1593 BglI GCCn_nnn'nGGC Cuts at: 0 541 1532 1604 1696 1805 3504 Size: 541 991 72 92 109 1699 Fragments arranged by size: 1699 991 541 109 92 72 BglII A'GATC_T Cuts at: 0 1106 1271 3504 Size: 1106 165 2233 Fragments arranged by size: 2233 1106 165 BlpI GC'TnA_GC Cuts at: 0 2738 3504 Size: 2738 766 Bme1580I G_kGCm'C Cuts at: 0 178 190 335 724 1186 1442 1840 2028 Size: 178 12 145 389 462 256 398 188 Cuts at: 2028 2220 2416 3271 3496 3504 Size: 192 196 855 225 8 Fragments arranged by size: 855 462 398 389 256 225 196 192 188 178 145 12 8 BmgBI CAC'GTC Cuts at: 0 2865 3504 Size: 2865 639 BmrI ACTGGGnnnn_n' Cuts at: 0 1173 3265 3504 Size: 1173 2092 239 Fragments arranged by size: 2092 1173 239 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1389 3504 Size: 1389 2115 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 427 3004 3448 3504 Size: 427 2577 444 56 Fragments arranged by size: 2577 444 427 56 Bpu10I CC'TnA_GC Cuts at: 0 311 527 784 1882 3504 Size: 311 216 257 1098 1622 Fragments arranged by size: 1622 1098 311 257 216 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1609 1990 3504 Size: 1609 381 1514 Fragments arranged by size: 1609 1514 381 BsaI GGTCTCn'nnnn_ Cuts at: 0 952 2629 3504 Size: 952 1677 875 Fragments arranged by size: 1677 952 875 BsaAI yAC'GTr Cuts at: 0 639 1384 3504 Size: 639 745 2120 Fragments arranged by size: 2120 745 639 BsaBI GATnn'nnATC Cuts at: 0 1270 3504 Size: 1270 2234 BsaHI Gr'CG_yC Cuts at: 0 87 203 319 357 1448 1661 2609 3504 Size: 87 116 116 38 1091 213 948 895 Fragments arranged by size: 1091 948 895 213 116 116 87 38 BsaWI w'CCGG_w Cuts at: 0 212 2831 3504 Size: 212 2619 673 Fragments arranged by size: 2619 673 212 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 400 430 2000 2030 3504 Size: 400 30 1570 30 1474 Fragments arranged by size: 1570 1474 400 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 745 1696 2818 2827 3076 3504 Size: 745 951 1122 9 249 428 Fragments arranged by size: 1122 951 745 428 249 9 BseYI C'CCAG_C Cuts at: 0 157 243 645 1040 1748 2562 2571 3496 Size: 157 86 402 395 708 814 9 925 Cuts at: 3496 3504 Size: 8 Fragments arranged by size: 925 814 708 402 395 157 86 9 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 99 388 685 876 897 2336 2476 3504 Size: 99 289 297 191 21 1439 140 1028 Fragments arranged by size: 1439 1028 297 289 191 140 99 21 BsiEI CG_ry'CG Cuts at: 0 3372 3504 Size: 3372 132 BsiHKAI G_wGCw'C Cuts at: 0 1186 1220 1442 3227 3504 Size: 1186 34 222 1785 277 Fragments arranged by size: 1785 1186 277 222 34 Bsp1286I G_dGCh'C Cuts at: 0 178 190 335 581 724 1186 1220 1442 Size: 178 12 145 246 143 462 34 222 Cuts at: 1442 1840 2028 2220 2416 2540 3227 3271 3297 Size: 398 188 192 196 124 687 44 26 Cuts at: 3297 3496 3504 Size: 199 8 Fragments arranged by size: 687 462 398 246 222 199 196 192 188 178 145 143 124 44 34 26 12 8 BspEI T'CCGG_A Cuts at: 0 212 3504 Size: 212 3292 BspHI T'CATG_A Cuts at: 0 1202 3504 Size: 1202 2302 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 94 360 657 998 2349 2372 2434 2733 Size: 94 266 297 341 1351 23 62 299 Cuts at: 2733 3271 3504 Size: 538 233 Fragments arranged by size: 1351 538 341 299 297 266 233 94 62 23 BsrBI CCG'CTC Cuts at: 0 1017 2210 3066 3158 3504 Size: 1017 1193 856 92 346 Fragments arranged by size: 1193 1017 856 346 92 BsrDI GCAATG_nn' Cuts at: 0 72 2270 2904 2951 3504 Size: 72 2198 634 47 553 Fragments arranged by size: 2198 634 553 72 47 BsrFI r'CCGG_y Cuts at: 0 596 1937 3504 Size: 596 1341 1567 Fragments arranged by size: 1567 1341 596 BstEII G'GTnAC_C Cuts at: 0 3381 3504 Size: 3381 123 BstXI CCAn_nnnn'nTGG Cuts at: 0 627 1377 1512 1728 2865 3504 Size: 627 750 135 216 1137 639 Fragments arranged by size: 1137 750 639 627 216 135 BstYI r'GATC_y Cuts at: 0 1106 1271 1965 1995 2096 3504 Size: 1106 165 694 30 101 1408 Fragments arranged by size: 1408 1106 694 165 101 30 Bsu36I CC'TnA_GG Cuts at: 0 1261 1859 2174 3120 3504 Size: 1261 598 315 946 384 Fragments arranged by size: 1261 946 598 384 315 BtgI C'CryG_G Cuts at: 0 90 837 963 1052 1599 1727 2613 3386 Size: 90 747 126 89 547 128 886 773 Cuts at: 3386 3504 Size: 118 Fragments arranged by size: 886 773 747 547 128 126 118 90 89 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 726 3018 3504 Size: 726 2292 486 Fragments arranged by size: 2292 726 486 BtsI GCAGTG_nn' Cuts at: 0 263 668 1184 1254 1332 1440 1558 2351 Size: 263 405 516 70 78 108 118 793 Cuts at: 2351 3167 3504 Size: 816 337 Fragments arranged by size: 816 793 516 405 337 263 118 108 78 70 ClaI AT'CG_AT Cuts at: 0 1895 3504 Size: 1895 1609 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2565 2600 3504 Size: 2565 35 904 Fragments arranged by size: 2565 904 35 EaeI y'GGCC_r Cuts at: 0 398 1502 1533 1697 2474 2516 3504 Size: 398 1104 31 164 777 42 988 Fragments arranged by size: 1104 988 777 398 164 42 31 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 598 1786 3504 Size: 598 1188 1718 Fragments arranged by size: 1718 1188 598 EcoICRI GAG'CTC Cuts at: 0 1218 3504 Size: 1218 2286 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 427 3004 3448 3504 Size: 427 2577 444 56 Fragments arranged by size: 2577 444 427 56 EcoNI CCTnn'n_nnAGG Cuts at: 0 2407 3397 3504 Size: 2407 990 107 Fragments arranged by size: 2407 990 107 EcoO109I rG'GnC_Cy Cuts at: 0 175 2178 2566 3363 3492 3493 3504 Size: 175 2003 388 797 129 1 11 Fragments arranged by size: 2003 797 388 175 129 11 1 FspI TGC'GCA Cuts at: 0 1190 3504 Size: 1190 2314 HaeII r_GCGC'y Cuts at: 0 90 322 360 414 680 945 1664 2725 Size: 90 232 38 54 266 265 719 1061 Cuts at: 2725 2895 3504 Size: 170 609 Fragments arranged by size: 1061 719 609 266 265 232 170 90 54 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1468 1500 1950 1982 3428 3460 3504 Size: 1468 32 450 32 1446 32 44 Fragments arranged by size: 1468 1446 450 44 32 32 32 HincII GTy'rAC Cuts at: 0 2753 3504 Size: 2753 751 KasI G'GCGC_C Cuts at: 0 86 318 356 1660 3504 Size: 86 232 38 1304 1844 Fragments arranged by size: 1844 1304 232 86 38 MluI A'CGCG_T Cuts at: 0 1616 3504 Size: 1616 1888 MscI TGG'CCA Cuts at: 0 1504 1699 2476 2518 3504 Size: 1504 195 777 42 986 Fragments arranged by size: 1504 986 777 195 42 MslI CAynn'nnrTG Cuts at: 0 490 586 625 766 1057 1127 1179 1337 Size: 490 96 39 141 291 70 52 158 Cuts at: 1337 1414 2086 3109 3218 3504 Size: 77 672 1023 109 286 Fragments arranged by size: 1023 672 490 291 286 158 141 109 96 77 70 52 39 MspA1I CmG'CkG Cuts at: 0 298 423 490 788 965 1430 1710 1917 Size: 298 125 67 298 177 465 280 207 Cuts at: 1917 2662 3078 3426 3504 Size: 745 416 348 78 Fragments arranged by size: 745 465 416 348 298 298 280 207 177 125 78 67 NarI GG'CG_CC Cuts at: 0 87 319 357 1661 3504 Size: 87 232 38 1304 1843 Fragments arranged by size: 1843 1304 232 87 38 NcoI C'CATG_G Cuts at: 0 1052 1727 2613 3504 Size: 1052 675 886 891 Fragments arranged by size: 1052 891 886 675 NspI r_CATG'y Cuts at: 0 771 1413 2283 2460 2737 3504 Size: 771 642 870 177 277 767 Fragments arranged by size: 870 771 767 642 277 177 PasI CC'CwG_GG Cuts at: 0 264 3504 Size: 264 3240 PciI A'CATG_T Cuts at: 0 2279 3504 Size: 2279 1225 PflMI CCAn_nnn'nTGG Cuts at: 0 1600 2619 2880 3504 Size: 1600 1019 261 624 Fragments arranged by size: 1600 1019 624 261 PfoI T'CCnGG_A Cuts at: 0 140 1771 1984 3504 Size: 140 1631 213 1520 Fragments arranged by size: 1631 1520 213 140 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 3052 3084 3504 Size: 3052 32 420 Fragments arranged by size: 3052 420 32 PpuMI rG'GwC_Cy Cuts at: 0 2566 3363 3504 Size: 2566 797 141 Fragments arranged by size: 2566 797 141 PspOMI G'GGCC_C Cuts at: 0 174 3267 3492 3504 Size: 174 3093 225 12 Fragments arranged by size: 3093 225 174 12 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1564 1596 3504 Size: 1564 32 1908 Fragments arranged by size: 1908 1564 32 PstI C_TGCA'G Cuts at: 0 995 1565 1709 2369 2431 2667 3504 Size: 995 570 144 660 62 236 837 Fragments arranged by size: 995 837 660 570 236 144 62 PvuII CAG'CTG Cuts at: 0 1917 2662 3078 3426 3504 Size: 1917 745 416 348 78 Fragments arranged by size: 1917 745 416 348 78 SacI G_AGCT'C Cuts at: 0 1220 3504 Size: 1220 2284 SacII CC_GC'GG Cuts at: 0 966 3504 Size: 966 2538 SalI G'TCGA_C Cuts at: 0 2751 3504 Size: 2751 753 SanDI GG'GwC_CC Cuts at: 0 2566 3363 3504 Size: 2566 797 141 Fragments arranged by size: 2566 797 141 ScaI AGT'ACT Cuts at: 0 1981 2443 3504 Size: 1981 462 1061 Fragments arranged by size: 1981 1061 462 SexAI A'CCwGG_T Cuts at: 0 3398 3504 Size: 3398 106 SfcI C'TryA_G Cuts at: 0 991 1561 1705 2365 2427 2663 2782 2818 Size: 991 570 144 660 62 236 119 36 Cuts at: 2818 3504 Size: 686 Fragments arranged by size: 991 686 660 570 236 144 119 62 36 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1532 1696 3504 Size: 1532 164 1808 Fragments arranged by size: 1808 1532 164 SfoI GGC'GCC Cuts at: 0 88 320 358 1662 3504 Size: 88 232 38 1304 1842 Fragments arranged by size: 1842 1304 232 88 38 SmaI CCC'GGG Cuts at: 0 1318 2887 3504 Size: 1318 1569 617 Fragments arranged by size: 1569 1318 617 SmlI C'TyrA_G Cuts at: 0 1624 1969 3504 Size: 1624 345 1535 Fragments arranged by size: 1624 1535 345 SphI G_CATG'C Cuts at: 0 771 2737 3504 Size: 771 1966 767 Fragments arranged by size: 1966 771 767 SrfI GCCC'GGGC Cuts at: 0 1318 3504 Size: 1318 2186 StuI AGG'CCT Cuts at: 0 949 1792 3504 Size: 949 843 1712 Fragments arranged by size: 1712 949 843 StyI C'CwwG_G Cuts at: 0 1052 1452 1727 1778 1787 2613 2858 3413 Size: 1052 400 275 51 9 826 245 555 Cuts at: 3413 3504 Size: 91 Fragments arranged by size: 1052 826 555 400 275 245 91 51 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 171 615 1855 3386 3504 Size: 171 444 1240 1531 118 Fragments arranged by size: 1531 1240 444 171 118 TatI w'GTAC_w Cuts at: 0 1979 2150 2441 3504 Size: 1979 171 291 1063 Fragments arranged by size: 1979 1063 291 171 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 607 639 906 938 3504 Size: 607 32 267 32 2566 Fragments arranged by size: 2566 607 267 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1129 3504 Size: 1129 2375 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 3195 3250 3504 Size: 3195 55 254 Fragments arranged by size: 3195 254 55 XmaI C'CCGG_G Cuts at: 0 1316 2885 3504 Size: 1316 1569 619 Fragments arranged by size: 1569 1316 619 XmnI GAAnn'nnTTC Cuts at: 0 388 3504 Size: 388 3116 Enzymes that do cut: AarI AccI AflIII AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII KasI MluI MscI MslI MspA1I NarI NcoI NspI PasI PciI PflMI PfoI PpiI PpuMI PspOMI PsrI PstI PvuII SacI SacII SalI SanDI ScaI SexAI SfcI SfiI SfoI SmaI SmlI SphI SrfI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI AloI AscI AseI AsiSI AvrII BfrBI BmtI BsiWI BsmI BsmBI BsrGI BssHII BssSI BstAPI BstBI BstZ17I DraI DraIII DrdI EagI EarI EcoRI EcoRV FalI FseI FspAI HindIII HpaI KpnI MfeI MmeI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PshAI PsiI PspXI PvuI RsrII SapI SbfI SgfI SgrAI SnaBI SpeI SspI SwaI XbaI XhoI ZraI