################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## AatII AclI AflII AflIII AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AvrII BamHI BbrPI BbvCI BclI BfrI BglII BlnI BsgI BsiWI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI Eam1105I Eco47III Eco81I EcoRV EcoT22I FbaI FseI HindIII HpaI KspI MfeI MluI MunI NaeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SbfI ScaI SfiI SfuI SnaBI SpeI SphI Sse8387I SspBI Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### Acc65I 2536 GGTACC G^GTACC AccI 1354 GTCGAC GT^[AC][GT]AC AccII 514 CGCG CG^CG AccIII 1363 TCCGGA T^CCGGA AcyI 2020 GGCGCC G[GA]^CG[CT]C AgeI 1661 ACCGGT A^CCGGT Asp700I 2703 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2536 GGTACC G^GTACC AspI 1974 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1432 TGCGCA TGC^GCA BbeI 2020 GGCGCC GGCGC^C BcgI 2186 GCATTCGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 509 GTATCC GTATCC(6/5) BglI 1141 GCCATGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1653 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1653 GCTGAGC GC^T[ACGT]AGC BsaAI 2175 TACGTG [CT]AC^GT[GA] BsaBI 201 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2020 GGCGCC G[GA]^CG[CT]C BseAI 1363 TCCGGA T^CCGGA BsmI 2186 GCATTC GAATGC(1/-1) BspEI 1363 TCCGGA T^CCGGA BspMI 1938 ACCTGC ACCTGC(4/8) BsrFI 1661 ACCGGT [GA]^CCGG[CT] BstAPI 1465 GCAGCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 514 CGCG CG^CG CelII 1653 GCTGAGC GC^T[ACGT]AGC Cfr10I 1661 ACCGGT [GA]^CCGG[CT] DraI 718 TTTAAA TTT^AAA DraIII 521 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 1707 CGGCCG C^GGCCG EclXI 1707 CGGCCG C^GGCCG Eco52I 1707 CGGCCG C^GGCCG EcoNI 84 CCTTACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 1432 TGCGCA TGC^GCA HaeII 2020 GGCGCC [GA]GCGC^[CT] HgaI 2489 GCGTC GACGC(5/10) Hin1I 2020 GGCGCC G[GA]^CG[CT]C KasI 2020 GGCGCC G^GCGCC KpnI 2536 GGTACC GGTAC^C MamI 201 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1363 TCCGGA T^CCGGA MvnI 514 CGCG CG^CG NarI 2020 GGCGCC GG^CGCC NdeI 168 CATATG CA^TATG PflFI 1974 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 1661 ACCGGT A^CCGGT PpuMI 1585 AGGTCCC [GA]G^G[AT]CC[CT] PshAI 2484 GACAAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 1267 GAGCTC GAGCT^C SalI 1354 GTCGAC G^TCGAC SapI 951 GAAGAGC GCTCTTC(1/4) SexAI 522 ACCAGGT A^CC[AT]GGT SfoI 2020 GGCGCC GGC^GCC SgrAI 1660 CACCGGTG C[GA]^CCGG[CT]G SmaI 2576 CCCGGG CCC^GGG SspI 76 AATATT AAT^ATT StuI 2718 AGGCCT AGG^CCT SwaI 717 ATTTAAAT ATTT^AAAT Tth111I 1974 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 2576 CCCGGG C^CCGGG XmaI 2576 CCCGGG C^CCGGG XmnI 2703 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AlwI 2526 GATCC GGATC(4/5) 2640 GGATC AspHI 1097 GTGCTC G[AT]GC[AT]^C 1267 GAGCTC BalI 468 TGGCCA TGG^CCA 782 TGGCCA BanI 2020 GGCGCC G^G[CT][GA]CC 2536 GGTACC BfaI 659 CTAG C^TAG 823 CTAG BpmI 242 CTCCAG CTGGAG(16/14) 2521 CTGGAG BsaI 259 GAGACC GGTCTC(1/5) 2580 GGTCTC BsaWI 1363 TCCGGA [AT]^CCGG[AT] 1661 ACCGGT BsiEI 1707 CGGCCG CG[GA][CT]^CG 2046 CGACCG BsiHKAI 1097 GTGCTC G[AT]GC[AT]^C 1267 GAGCTC BsmBI 1012 CGTCTC CGTCTC(1/5) 2692 GAGACG BsrBI 239 CCGCTC CCGCTC(-3/-3) 1320 CCGCTC BssSI 1375 CACGAG CACGAG(-5/-1) 1270 CTCGTG BstXI 1169 CCAACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1289 CCAACCCTGTGG BstYI 2525 AGATCC [GA]^GATC[CT] 2640 GGATCT DrdI 900 GACTGCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1753 GACATGGGTGTC EarI 34 CTCTTC CTCTTC(1/4) 951 GAAGAG EcoRI 1006 GAATTC G^AATTC 1342 GAATTC Ksp632I 34 CTCTTC CTCTTC(1/4) 951 GAAGAG MaeI 659 CTAG C^TAG 823 CTAG MaeII 2176 ACGT A^CGT 2246 ACGT MflI 2525 AGATCC [GA]^GATC[CT] 2640 GGATCT MluNI 468 TGGCCA TGG^CCA 782 TGGCCA MscI 468 TGGCCA TGG^CCA 782 TGGCCA NcoI 1175 CCATGG C^CATGG 1326 CCATGG SmlI 973 CTTGAG C^T[CT][GA]AG 1581 CTCAAG XhoII 2525 AGATCC [GA]^GATC[CT] 2640 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AvaI AvaII BanII BbsI BbvI BcnI BmyI BpuAI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstEII BstF5I BstNI BstPI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI