(Circular) (Six-base) MAPSORT of: pFN21AE2711.seq Check: 922 from: 1 to: 7498 pFN21AE2711 7498 bp With 182 enzymes: SgfI * January 19, 2018 17:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5308 278 Size: 53 83 186 936 3772 2468 Fragments arranged by size: 3772 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3360 4841 1937 Size: 1423 1481 4594 Fragments arranged by size: 4594 1481 1423 Acc65I G'GTAC_C Cuts at: 4541 4819 4541 Size: 278 7220 AclI AA'CG_TT Cuts at: 1563 5625 5998 1563 Size: 4062 373 3063 Fragments arranged by size: 4062 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2642 2787 2916 3222 4748 5561 6945 7213 Size: 1010 145 129 306 1526 813 1384 268 Cuts at: 7213 1632 Size: 1917 Fragments arranged by size: 1917 1526 1384 1010 813 306 268 145 129 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7301 AflIII A'CryG_T Cuts at: 1203 6397 7374 1203 Size: 5194 977 1327 Fragments arranged by size: 5194 1327 977 AgeI A'CCGG_T Cuts at: 3666 6329 3666 Size: 2663 4835 AhdI GACnn_n'nnGTC Cuts at: 6227 6227 Size: 7498 AleI CACnn'nnGTG Cuts at: 1166 4127 1166 Size: 2961 4537 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7498 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3039 3071 3039 Size: 32 7466 AlwNI CAG_nnn'CTG Cuts at: 5241 6813 5241 Size: 1572 5926 ApaLI G'TGCA_C Cuts at: 5555 6711 5555 Size: 1156 6342 ApoI r'AATT_y Cuts at: 1493 2068 2726 3011 3347 4807 5033 5185 7294 Size: 575 658 285 336 1460 226 152 2109 Cuts at: 7294 1493 Size: 1697 Fragments arranged by size: 2109 1697 1460 658 575 336 285 226 152 AseI AT'TA_AT Cuts at: 160 1032 6052 160 Size: 872 5020 1606 Fragments arranged by size: 5020 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7498 AvaI C'yCGr_G Cuts at: 1870 1943 2100 4199 4581 4823 1870 Size: 73 157 2099 382 242 4545 Fragments arranged by size: 4545 2099 382 242 157 73 BamHI G'GATC_C Cuts at: 4828 5195 4828 Size: 367 7131 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4025 4541 4819 6274 618 Size: 325 217 627 2238 516 278 1455 1842 Fragments arranged by size: 2238 1842 1455 627 516 325 278 217 BanII G_rGCy'C Cuts at: 1391 2963 3082 3276 3387 4817 1391 Size: 1572 119 194 111 1430 4072 Fragments arranged by size: 4072 1572 1430 194 119 111 BbeI G_GCGC'C Cuts at: 4029 4029 Size: 7498 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2909 4084 4484 928 Size: 971 1010 1175 400 3942 Fragments arranged by size: 3942 1175 1010 971 400 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4180 4214 5689 5723 1903 Size: 34 2243 34 1475 34 3678 Fragments arranged by size: 3678 2243 1475 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2525 5390 6600 2525 Size: 2865 1210 3423 Fragments arranged by size: 3423 2865 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7498 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3152 6109 136 Size: 107 122 71 631 605 1480 2957 1525 Fragments arranged by size: 2957 1525 1480 631 605 122 107 71 BglII A'GATC_T Cuts at: 7493 7493 Size: 7498 BlpI GC'TnA_GC Cuts at: 3659 4915 3659 Size: 1256 6242 Bme1580I G_kGCm'C Cuts at: 1790 4273 5559 6715 1790 Size: 2483 1286 1156 2573 Fragments arranged by size: 2573 2483 1286 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7498 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2650 3087 3539 Size: 416 53 83 399 24 1233 437 452 Cuts at: 3539 4787 5229 6187 442 Size: 1248 442 958 1753 Fragments arranged by size: 1753 1248 1233 958 452 442 437 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7498 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2575 3830 3980 2575 Size: 1255 150 6093 Fragments arranged by size: 6093 1255 150 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2232 4547 6158 1239 Size: 993 2315 1611 2579 Fragments arranged by size: 2579 2315 1611 993 Bpu10I CC'TnA_GC Cuts at: 3948 3948 Size: 7498 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2999 3571 5623 6488 6786 2999 Size: 572 2052 865 298 3711 Fragments arranged by size: 3711 2052 865 572 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2258 4591 6161 882 Size: 617 759 2333 1570 2219 Fragments arranged by size: 2333 2219 1570 759 617 BsaAI yAC'GTr Cuts at: 493 1204 4182 5289 493 Size: 711 2978 1107 2702 Fragments arranged by size: 2978 2702 1107 711 BsaBI GATnn'nnATC Cuts at: 2210 7492 2210 Size: 5282 2216 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4026 5305 5687 6322 Size: 53 83 186 936 2493 1279 382 635 Cuts at: 6322 275 Size: 1451 Fragments arranged by size: 2493 1451 1279 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3368 3666 5931 6329 6603 6750 7161 3368 Size: 298 2265 398 274 147 411 3705 Fragments arranged by size: 3705 2265 411 398 298 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2152 2182 3311 3341 3819 Size: 30 165 30 749 30 1129 30 478 Cuts at: 3819 3849 1178 Size: 30 4827 Fragments arranged by size: 4827 1129 749 478 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2047 3366 3569 3572 3969 1186 Size: 861 1319 203 3 397 4715 Fragments arranged by size: 4715 1319 861 397 203 3 BseYI C'CCAG_C Cuts at: 2761 3107 3427 4255 6701 7197 2761 Size: 346 320 828 2446 496 3062 Fragments arranged by size: 3062 2446 828 496 346 320 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6925 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3715 4054 4948 5709 6737 1540 Size: 275 186 1714 339 894 761 1028 2301 Fragments arranged by size: 2301 1714 1028 894 761 339 275 186 BsiHKAI G_wGCw'C Cuts at: 3106 3276 5559 5644 6715 3106 Size: 170 2283 85 1071 3889 Fragments arranged by size: 3889 2283 1071 170 85 BsmI GAATG_Cn' Cuts at: 4191 5009 5102 4191 Size: 818 93 6587 Fragments arranged by size: 6587 818 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3023 4691 3023 Size: 1668 5830 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2963 3082 3106 3276 3387 4273 4817 Size: 399 1173 119 24 170 111 886 544 Cuts at: 4817 5559 5644 6715 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1173 1071 886 742 544 399 170 119 111 85 24 BspEI T'CCGG_A Cuts at: 3368 7161 3368 Size: 3793 3705 BspHI T'CATG_A Cuts at: 5387 7117 5387 Size: 1730 5768 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3952 4853 844 Size: 3108 901 3489 Fragments arranged by size: 3489 3108 901 BsrBI CCG'CTC Cuts at: 1394 1823 2246 3327 5385 1394 Size: 429 423 1081 2058 3507 Fragments arranged by size: 3507 2058 1081 429 423 BsrDI GCAATG_nn' Cuts at: 65 1248 5993 6167 65 Size: 1183 4745 174 1396 Fragments arranged by size: 4745 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3666 6142 6329 1700 Size: 110 1856 2476 187 2869 Fragments arranged by size: 2869 2476 1856 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7498 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7498 BssSI C'ACGA_G Cuts at: 3275 3380 5558 6570 3275 Size: 105 2178 1012 4203 Fragments arranged by size: 4203 2178 1012 105 BstAPI GCAn_nnn'nTGC Cuts at: 794 3476 794 Size: 2682 4816 BstBI TT'CG_AA Cuts at: 6302 6302 Size: 7498 BstEII G'GTnAC_C Cuts at: 1184 2329 3538 4322 1184 Size: 1145 1209 784 4360 Fragments arranged by size: 4360 1209 1145 784 BstXI CCAn_nnnn'nTGG Cuts at: 3181 3301 3181 Size: 120 7378 BstYI r'GATC_y Cuts at: 1972 4530 4645 4828 5195 5580 5597 7049 7135 Size: 2558 115 183 367 385 17 1452 86 Cuts at: 7135 7147 7493 1972 Size: 12 346 1977 Fragments arranged by size: 2558 1977 1452 385 367 346 183 115 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2582 3180 3331 4565 513 Size: 552 939 578 598 151 1234 3446 Fragments arranged by size: 3446 1234 939 598 578 552 151 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6759 Fragments arranged by size: 6759 683 56 BtsI GCAGTG_nn' Cuts at: 2972 3078 4005 5014 5808 5828 2972 Size: 106 927 1009 794 20 4642 Fragments arranged by size: 4642 1009 927 794 106 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7498 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2833 2868 2959 2994 4137 4172 529 Size: 35 2269 35 91 35 1143 35 3855 Fragments arranged by size: 3855 2269 1143 91 35 35 35 35 DraI TTT'AAA Cuts at: 2725 4802 2725 Size: 2077 5421 DraIII CAC_nnn'GTG Cuts at: 2531 2531 Size: 7498 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2911 3764 6505 809 Size: 507 1595 853 2741 1802 Fragments arranged by size: 2741 1802 1595 853 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2473 2746 Size: 54 1425 50 275 18 177 466 273 Cuts at: 2746 2787 3132 3712 3726 4701 4945 5834 7463 Size: 41 345 580 14 975 244 889 1629 Cuts at: 7463 8 Size: 43 Fragments arranged by size: 1629 1425 975 889 580 466 345 275 273 244 177 54 50 43 41 18 14 EagI C'GGCC_G Cuts at: 1537 1812 3712 4945 1537 Size: 275 1900 1233 4090 Fragments arranged by size: 4090 1900 1233 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2045 2951 5428 1353 Size: 692 906 2477 3423 Fragments arranged by size: 3423 2477 906 692 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6085 6459 6605 387 Size: 1064 138 4496 374 146 1280 Fragments arranged by size: 4496 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3274 3274 Size: 7498 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2232 2642 2787 2916 3222 4547 4748 Size: 393 600 410 145 129 306 1325 201 Cuts at: 4748 5561 6158 6945 7213 1239 Size: 813 597 787 268 1524 Fragments arranged by size: 1524 1325 813 787 600 597 410 393 306 268 201 145 129 EcoNI CCTnn'n_nnAGG Cuts at: 2093 2093 Size: 7498 EcoO109I rG'GnC_Cy Cuts at: 3035 3154 3591 3035 Size: 119 437 6942 Fragments arranged by size: 6942 437 119 EcoRI G'AATT_C Cuts at: 3011 3347 4807 5185 3011 Size: 336 1460 378 5324 Fragments arranged by size: 5324 1460 378 336 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4729 4729 Size: 7498 FspI TGC'GCA Cuts at: 3439 6004 3439 Size: 2565 4933 HaeII r_GCGC'y Cuts at: 1678 4029 6645 1678 Size: 2351 2616 2531 Fragments arranged by size: 2616 2531 2351 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2152 2184 2879 2911 3604 Size: 32 135 32 580 32 695 32 693 Cuts at: 3604 3636 3819 3851 3861 3893 6139 6171 6213 Size: 32 183 32 10 32 2246 32 42 Cuts at: 6213 6245 1373 Size: 32 2626 Fragments arranged by size: 2626 2246 695 693 580 183 135 42 32 32 32 32 32 32 32 32 32 10 HincII GTy'rAC Cuts at: 669 1657 1938 2461 3361 4278 4423 4498 4842 Size: 988 281 523 900 917 145 75 344 Cuts at: 4842 5088 669 Size: 246 3079 Fragments arranged by size: 3079 988 917 900 523 344 281 246 145 75 HindIII A'AGCT_T Cuts at: 748 748 Size: 7498 HpaI GTT'AAC Cuts at: 5088 5088 Size: 7498 KasI G'GCGC_C Cuts at: 4025 4025 Size: 7498 KpnI G_GTAC'C Cuts at: 4545 4823 4545 Size: 278 7220 MfeI C'AATT_G Cuts at: 5097 5097 Size: 7498 MluI A'CGCG_T Cuts at: 7374 7374 Size: 7498 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6612 6796 1305 Size: 5307 184 2007 Fragments arranged by size: 5307 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2475 2789 10 Size: 54 1425 343 643 314 4719 Fragments arranged by size: 4719 1425 643 343 314 54 MslI CAynn'nnrTG Cuts at: 518 1166 2433 2552 3737 4127 5456 5815 5974 Size: 648 1267 119 1185 390 1329 359 159 Cuts at: 5974 518 Size: 2042 Fragments arranged by size: 2042 1329 1267 1185 648 390 359 159 119 MspA1I CmG'CkG Cuts at: 1606 2252 2620 2637 2765 4914 5591 6739 6984 Size: 646 368 17 128 2149 677 1148 245 Cuts at: 6984 1606 Size: 2120 Fragments arranged by size: 2149 2120 1148 677 646 368 245 128 17 NarI GG'CG_CC Cuts at: 4026 4026 Size: 7498 NcoI C'CATG_G Cuts at: 513 1065 2004 3180 3331 513 Size: 552 939 1176 151 4680 Fragments arranged by size: 4680 1176 939 552 151 NdeI CA'TA_TG Cuts at: 387 2174 387 Size: 1787 5711 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7498 NotI GC'GGCC_GC Cuts at: 4945 4945 Size: 7498 NspI r_CATG'y Cuts at: 1132 1229 4856 6401 1132 Size: 97 3627 1545 2229 Fragments arranged by size: 3627 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7498 PciI A'CATG_T Cuts at: 6397 6397 Size: 7498 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7498 PfoI T'CCnGG_A Cuts at: 3863 4472 4533 3863 Size: 609 61 6828 Fragments arranged by size: 6828 609 61 PmeI GTTT'AAAC Cuts at: 4802 4802 Size: 7498 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3374 3406 5567 5599 7080 7112 3374 Size: 32 2161 32 1481 32 3760 Fragments arranged by size: 3760 2161 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3591 3591 Size: 7498 PshAI GACnn'nnGTC Cuts at: 1613 4493 1613 Size: 2880 4618 PsiI TTA'TAA Cuts at: 5068 5068 Size: 7498 PstI C_TGCA'G Cuts at: 830 2911 4049 4293 4850 830 Size: 2081 1138 244 557 3478 Fragments arranged by size: 3478 2081 1138 557 244 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7498 PvuII CAG'CTG Cuts at: 2252 2620 2765 2252 Size: 368 145 6985 Fragments arranged by size: 6985 368 145 SacI G_AGCT'C Cuts at: 3276 3276 Size: 7498 SalI G'TCGA_C Cuts at: 1936 3359 4840 1936 Size: 1423 1481 4594 Fragments arranged by size: 4594 1481 1423 SapI GCTCTTCn'nnn_ Cuts at: 2951 2951 Size: 7498 SbfI CC_TGCA'GG Cuts at: 4850 4850 Size: 7498 ScaI AGT'ACT Cuts at: 5746 7458 5746 Size: 1712 5786 SexAI A'CCwGG_T Cuts at: 2527 2527 Size: 7498 SfcI C'TryA_G Cuts at: 826 1045 2149 2566 2907 3889 4045 4225 4289 Size: 219 1104 417 341 982 156 180 64 Cuts at: 4289 4846 5981 6662 6853 826 Size: 557 1135 681 191 1471 Fragments arranged by size: 1471 1135 1104 982 681 557 417 341 219 191 180 156 64 SfoI GGC'GCC Cuts at: 4027 4027 Size: 7498 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7498 SgrAI Cr'CCGG_yG Cuts at: 1700 3666 1700 Size: 1966 5532 SmaI CCC'GGG Cuts at: 1872 4583 4825 1872 Size: 2711 242 4545 Fragments arranged by size: 4545 2711 242 SmlI C'TyrA_G Cuts at: 820 1017 1943 2978 3586 5602 6503 6765 820 Size: 197 926 1035 608 2016 901 262 1553 Fragments arranged by size: 2016 1553 1035 926 901 608 262 197 SnaBI TAC'GTA Cuts at: 493 5289 493 Size: 4796 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7498 SphI G_CATG'C Cuts at: 1132 4856 1132 Size: 3724 3774 SspI AAT'ATT Cuts at: 5 52 2083 5422 5 Size: 47 2031 3339 2081 Fragments arranged by size: 3339 2081 2031 47 StuI AGG'CCT Cuts at: 1517 4725 1517 Size: 3208 4290 StyI C'CwwG_G Cuts at: 513 1065 2004 2094 2844 3180 3331 4125 4336 Size: 552 939 90 750 336 151 794 211 Cuts at: 4336 4431 4937 513 Size: 95 506 3074 Fragments arranged by size: 3074 939 794 750 552 506 336 211 151 95 90 SwaI ATTT'AAAT Cuts at: 2725 2725 Size: 7498 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3293 3752 5525 5542 5695 5880 6325 Size: 1255 1713 459 1773 17 153 185 445 Cuts at: 6325 325 Size: 1498 Fragments arranged by size: 1773 1713 1498 1255 459 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5744 7456 96 Size: 274 80 33 51 1443 3767 1712 138 Fragments arranged by size: 3767 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3781 3813 7458 7490 3781 Size: 32 3645 32 3789 Fragments arranged by size: 3789 3645 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3982 1316 Size: 2666 4832 XbaI T'CTAG_A Cuts at: 4834 4834 Size: 7498 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2341 3115 3407 4433 4552 2341 Size: 774 292 1026 119 5287 Fragments arranged by size: 5287 1026 774 292 119 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7498 XmaI C'CCGG_G Cuts at: 1870 4581 4823 1870 Size: 2711 242 4545 Fragments arranged by size: 4545 2711 242 XmnI GAAnn'nnTTC Cuts at: 4712 5627 4712 Size: 915 6583 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5306 276 Size: 53 83 186 936 3772 2468 Fragments arranged by size: 3772 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SrfI