(Linear) (Six-base) MAPSORT of: KIEE2565.seq Check: 2302 from: 1 to: 3456 KIEE2565.orf With 182 enzymes: SgfI * March 23, 2015 09:39 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 754 971 1970 2216 2815 3344 3362 3456 Size: 754 217 999 246 599 529 18 94 Fragments arranged by size: 999 754 599 529 246 217 94 18 AflIII A'CryG_T Cuts at: 0 1514 3456 Size: 1514 1942 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1865 3456 Size: 1865 1591 AlwNI CAG_nnn'CTG Cuts at: 0 622 2377 2440 3340 3456 Size: 622 1755 63 900 116 Fragments arranged by size: 1755 900 622 116 63 ApaLI G'TGCA_C Cuts at: 0 2948 3456 Size: 2948 508 ApoI r'AATT_y Cuts at: 0 304 3456 Size: 304 3152 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 965 998 3456 Size: 965 33 2458 Fragments arranged by size: 2458 965 33 BamHI G'GATC_C Cuts at: 0 3214 3456 Size: 3214 242 BanI G'GyrC_C Cuts at: 0 750 3349 3456 Size: 750 2599 107 Fragments arranged by size: 2599 750 107 BanII G_rGCy'C Cuts at: 0 2078 2476 3456 Size: 2078 398 980 Fragments arranged by size: 2078 980 398 BbeI G_GCGC'C Cuts at: 0 3353 3456 Size: 3353 103 BbsI GAAGACnn'nnnn_ Cuts at: 0 742 3265 3456 Size: 742 2523 191 Fragments arranged by size: 2523 742 191 BbvCI CC'TCA_GC Cuts at: 0 638 3456 Size: 638 2818 BfrBI ATG'CAT Cuts at: 0 25 3456 Size: 25 3431 BglI GCCn_nnn'nGGC Cuts at: 0 2688 3377 3456 Size: 2688 689 79 Fragments arranged by size: 2688 689 79 BglII A'GATC_T Cuts at: 0 2392 3456 Size: 2392 1064 BlpI GC'TnA_GC Cuts at: 0 2758 3456 Size: 2758 698 Bme1580I G_kGCm'C Cuts at: 0 2952 3456 Size: 2952 504 BmgBI CAC'GTC Cuts at: 0 2310 3456 Size: 2310 1146 BmrI ACTGGGnnnn_n' Cuts at: 0 1414 3456 Size: 1414 2042 BmtI G_CTAG'C Cuts at: 0 667 3456 Size: 667 2789 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1088 1166 3456 Size: 1088 78 2290 Fragments arranged by size: 2290 1088 78 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 26 450 1553 1595 1697 2373 2867 3074 Size: 26 424 1103 42 102 676 494 207 Cuts at: 3074 3456 Size: 382 Fragments arranged by size: 1103 676 494 424 382 207 102 42 26 Bpu10I CC'TnA_GC Cuts at: 0 386 638 1676 2174 3456 Size: 386 252 1038 498 1282 Fragments arranged by size: 1282 1038 498 386 252 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2592 3456 Size: 2592 864 BsaI GGTCTCn'nnnn_ Cuts at: 0 299 1984 3108 3456 Size: 299 1685 1124 348 Fragments arranged by size: 1685 1124 348 299 BsaHI Gr'CG_yC Cuts at: 0 3350 3456 Size: 3350 106 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 107 137 230 260 279 309 1551 1581 Size: 107 30 93 30 19 30 1242 30 Cuts at: 1581 2769 2799 3143 3173 3239 3269 3456 Size: 1188 30 344 30 66 30 187 Fragments arranged by size: 1242 1188 344 187 107 93 66 30 30 30 30 30 30 30 19 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 626 1933 2864 3096 3167 3275 3328 3456 Size: 626 1307 931 232 71 108 53 128 Fragments arranged by size: 1307 931 626 232 128 108 71 53 BseYI C'CCAG_C Cuts at: 0 1029 1215 1647 2382 2656 2737 3307 3456 Size: 1029 186 432 735 274 81 570 149 Fragments arranged by size: 1029 735 570 432 274 186 149 81 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1835 1923 3456 Size: 1835 88 1533 Fragments arranged by size: 1835 1533 88 BsiEI CG_ry'CG Cuts at: 0 1916 3456 Size: 1916 1540 BsiHKAI G_wGCw'C Cuts at: 0 202 2078 2410 2590 2952 3456 Size: 202 1876 332 180 362 504 Fragments arranged by size: 1876 504 362 332 202 180 Bsp1286I G_dGCh'C Cuts at: 0 202 2078 2410 2476 2590 2952 3456 Size: 202 1876 332 66 114 362 504 Fragments arranged by size: 1876 504 362 332 202 114 66 BspHI T'CATG_A Cuts at: 0 1862 3456 Size: 1862 1594 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2133 2535 2970 3249 3456 Size: 2133 402 435 279 207 Fragments arranged by size: 2133 435 402 279 207 BsrBI CCG'CTC Cuts at: 0 2807 3456 Size: 2807 649 BsrDI GCAATG_nn' Cuts at: 0 74 2324 3456 Size: 74 2250 1132 Fragments arranged by size: 2250 1132 74 BsrFI r'CCGG_y Cuts at: 0 1911 2932 3318 3456 Size: 1911 1021 386 138 Fragments arranged by size: 1911 1021 386 138 BstAPI GCAn_nnn'nTGC Cuts at: 0 3250 3456 Size: 3250 206 BstEII G'GTnAC_C Cuts at: 0 3403 3456 Size: 3403 53 BstXI CCAn_nnnn'nTGG Cuts at: 0 789 999 1107 1185 1227 1473 3456 Size: 789 210 108 78 42 246 1983 Fragments arranged by size: 1983 789 246 210 108 78 42 BstYI r'GATC_y Cuts at: 0 2392 3214 3437 3456 Size: 2392 822 223 19 Fragments arranged by size: 2392 822 223 19 Bsu36I CC'TnA_GG Cuts at: 0 560 1995 3456 Size: 560 1435 1461 Fragments arranged by size: 1461 1435 560 BtgI C'CryG_G Cuts at: 0 884 2822 3456 Size: 884 1938 634 Fragments arranged by size: 1938 884 634 BtsI GCAGTG_nn' Cuts at: 0 1351 2501 3456 Size: 1351 1150 955 Fragments arranged by size: 1351 1150 955 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2178 2213 3129 3164 3456 Size: 2178 35 916 35 292 Fragments arranged by size: 2178 916 292 35 35 DrdI GACnn_nn'nnGTC Cuts at: 0 2553 3456 Size: 2553 903 EaeI y'GGCC_r Cuts at: 0 355 863 1337 1463 1697 1913 2111 2729 Size: 355 508 474 126 234 216 198 618 Cuts at: 2729 2768 3320 3378 3456 Size: 39 552 58 78 Fragments arranged by size: 618 552 508 474 355 234 216 198 126 78 58 39 EagI C'GGCC_G Cuts at: 0 1913 3456 Size: 1913 1543 EarI CTCTTCn'nnn_ Cuts at: 0 308 686 3456 Size: 308 378 2770 Fragments arranged by size: 2770 378 308 EcoICRI GAG'CTC Cuts at: 0 2076 3456 Size: 2076 1380 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 26 450 754 971 1553 1595 1697 1970 Size: 26 424 304 217 582 42 102 273 Cuts at: 1970 2216 2373 2815 2867 3074 3344 3362 3456 Size: 246 157 442 52 207 270 18 94 Fragments arranged by size: 582 442 424 304 273 270 246 217 207 157 102 94 52 42 26 18 EcoNI CCTnn'n_nnAGG Cuts at: 0 781 907 949 1633 1675 2280 2849 3149 Size: 781 126 42 684 42 605 569 300 Cuts at: 3149 3245 3456 Size: 96 211 Fragments arranged by size: 781 684 605 569 300 211 126 96 42 42 EcoO109I rG'GnC_Cy Cuts at: 0 1644 3298 3456 Size: 1644 1654 158 Fragments arranged by size: 1654 1644 158 EcoRI G'AATT_C Cuts at: 0 304 3456 Size: 304 3152 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 87 1923 3201 3456 Size: 87 1836 1278 255 Fragments arranged by size: 1836 1278 255 87 HaeII r_GCGC'y Cuts at: 0 3340 3353 3456 Size: 3340 13 103 Fragments arranged by size: 3340 103 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 214 246 820 852 1054 1086 1132 1164 Size: 214 32 574 32 202 32 46 32 Cuts at: 1164 1258 1290 1551 1583 1623 1655 3143 3156 Size: 94 32 261 32 40 32 1488 13 Cuts at: 3156 3175 3188 3456 Size: 19 13 268 Fragments arranged by size: 1488 574 268 261 214 202 94 46 40 32 32 32 32 32 32 32 19 13 13 HincII GTy'rAC Cuts at: 0 1969 3456 Size: 1969 1487 KasI G'GCGC_C Cuts at: 0 3349 3456 Size: 3349 107 MfeI C'AATT_G Cuts at: 0 2939 3456 Size: 2939 517 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 105 322 405 3456 Size: 105 217 83 3051 Fragments arranged by size: 3051 217 105 83 MscI TGG'CCA Cuts at: 0 357 865 1339 1699 2113 2770 3380 3456 Size: 357 508 474 360 414 657 610 76 Fragments arranged by size: 657 610 508 474 414 360 357 76 MslI CAynn'nnrTG Cuts at: 0 437 2677 3456 Size: 437 2240 779 Fragments arranged by size: 2240 779 437 MspA1I CmG'CkG Cuts at: 0 478 550 1849 2248 2437 2566 2641 2887 Size: 478 72 1299 399 189 129 75 246 Cuts at: 2887 3456 Size: 569 Fragments arranged by size: 1299 569 478 399 246 189 129 75 72 NaeI GCC'GGC Cuts at: 0 1913 2934 3320 3456 Size: 1913 1021 386 136 Fragments arranged by size: 1913 1021 386 136 NarI GG'CG_CC Cuts at: 0 3350 3456 Size: 3350 106 NcoI C'CATG_G Cuts at: 0 884 3456 Size: 884 2572 NgoMIV G'CCGG_C Cuts at: 0 1911 2932 3318 3456 Size: 1911 1021 386 138 Fragments arranged by size: 1911 1021 386 138 NheI G'CTAG_C Cuts at: 0 663 3456 Size: 663 2793 NsiI A_TGCA'T Cuts at: 0 27 3456 Size: 27 3429 NspI r_CATG'y Cuts at: 0 876 1518 3049 3456 Size: 876 642 1531 407 Fragments arranged by size: 1531 876 642 407 PciI A'CATG_T Cuts at: 0 1514 3456 Size: 1514 1942 PflMI CCAn_nnn'nTGG Cuts at: 0 3426 3456 Size: 3426 30 PfoI T'CCnGG_A Cuts at: 0 149 3440 3456 Size: 149 3291 16 Fragments arranged by size: 3291 149 16 PpuMI rG'GwC_Cy Cuts at: 0 1644 3298 3456 Size: 1644 1654 158 Fragments arranged by size: 1654 1644 158 PshAI GACnn'nnGTC Cuts at: 0 11 2573 3456 Size: 11 2562 883 Fragments arranged by size: 2562 883 11 PstI C_TGCA'G Cuts at: 0 1832 2532 2646 3246 3456 Size: 1832 700 114 600 210 Fragments arranged by size: 1832 700 600 210 114 PvuII CAG'CTG Cuts at: 0 478 550 1849 2248 2437 2566 2887 3456 Size: 478 72 1299 399 189 129 321 569 Fragments arranged by size: 1299 569 478 399 321 189 129 72 SacI G_AGCT'C Cuts at: 0 2078 3456 Size: 2078 1378 SanDI GG'GwC_CC Cuts at: 0 3298 3456 Size: 3298 158 SbfI CC_TGCA'GG Cuts at: 0 3246 3456 Size: 3246 210 SfcI C'TryA_G Cuts at: 0 287 1828 2528 2642 3000 3242 3456 Size: 287 1541 700 114 358 242 214 Fragments arranged by size: 1541 700 358 287 242 214 114 SfiI GGCCn_nnn'nGGCC Cuts at: 0 3377 3456 Size: 3377 79 SfoI GGC'GCC Cuts at: 0 3351 3456 Size: 3351 105 SmlI C'TyrA_G Cuts at: 0 2571 3456 Size: 2571 885 SspI AAT'ATT Cuts at: 0 491 3039 3456 Size: 491 2548 417 Fragments arranged by size: 2548 491 417 StuI AGG'CCT Cuts at: 0 97 2025 2286 3456 Size: 97 1928 261 1170 Fragments arranged by size: 1928 1170 261 97 StyI C'CwwG_G Cuts at: 0 668 782 866 884 1034 1100 1178 1220 Size: 668 114 84 18 150 66 78 42 Cuts at: 1220 1382 1466 2810 3029 3206 3425 3456 Size: 162 84 1344 219 177 219 31 Fragments arranged by size: 1344 668 219 219 177 162 150 114 84 84 78 66 42 31 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 855 1131 1209 2271 2691 3456 Size: 855 276 78 1062 420 765 Fragments arranged by size: 1062 855 765 420 276 78 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 586 618 2012 2044 3239 3271 3456 Size: 586 32 1394 32 1195 32 185 Fragments arranged by size: 1394 1195 586 185 32 32 32 XmnI GAAnn'nnTTC Cuts at: 0 1583 3456 Size: 1583 1873 Enzymes that do cut: AcuI AflIII AlfI AlwNI ApaLI ApoI BaeI BamHI BanI BanII BbeI BbsI BbvCI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII KasI MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI PciI PflMI PfoI PpuMI PshAI PstI PvuII SacI SanDI SbfI SfcI SfiI SfoI SmlI SspI StuI StyI TaqII TstI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AloI ApaI AscI AseI AsiSI AvaI AvrII BcgI BciVI BclI BsaAI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BsrGI BssHII BssSI BstBI BstZ17I BtgZI ClaI DraI DraIII EciI EcoRV FseI FspI FspAI HindIII HpaI KpnI MluI NdeI NotI NruI PacI PasI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SapI ScaI SexAI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TatI Tth111I XbaI XcmI XhoI XmaI ZraI