(Circular) (Six-base) MAPSORT of: pFN21AE2549.seq Check: 671 from: 1 to: 9214 pFN21AE2549 9214 bp With 182 enzymes: SgfI * August 16, 2021 15:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4160 5155 5280 5542 6421 4160 Size: 995 125 262 879 6953 Fragments arranged by size: 6953 995 879 262 125 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2502 3090 4623 4806 Size: 53 83 186 936 966 588 1533 183 Cuts at: 4806 5631 7024 278 Size: 825 1393 2468 Fragments arranged by size: 2468 1533 1393 966 936 825 588 186 183 83 53 AccI GT'mk_AC Cuts at: 1937 4917 6557 1937 Size: 2980 1640 4594 Fragments arranged by size: 4594 2980 1640 Acc65I G'GTAC_C Cuts at: 6535 6535 Size: 9214 AclI AA'CG_TT Cuts at: 1563 7341 7714 1563 Size: 5778 373 3063 Fragments arranged by size: 5778 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2582 2662 3432 3554 4001 4306 4579 5321 Size: 950 80 770 122 447 305 273 742 Cuts at: 5321 5408 5525 5596 6230 6278 7277 8661 8929 Size: 87 117 71 634 48 999 1384 268 Cuts at: 8929 1632 Size: 1917 Fragments arranged by size: 1917 1384 999 950 770 742 634 447 305 273 268 122 117 87 80 71 48 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9017 AflIII A'CryG_T Cuts at: 1203 2356 8113 9090 1203 Size: 1153 5757 977 1327 Fragments arranged by size: 5757 1327 1153 977 AgeI A'CCGG_T Cuts at: 8045 8045 Size: 9214 AhdI GACnn_n'nnGTC Cuts at: 7943 7943 Size: 9214 AleI CACnn'nnGTG Cuts at: 1166 4124 1166 Size: 2958 6256 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 6017 1184 Size: 4833 4381 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4400 4432 4886 4918 5789 5821 4400 Size: 32 454 32 871 32 7793 Fragments arranged by size: 7793 871 454 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 3151 3250 3697 4114 6494 6957 8529 3151 Size: 99 447 417 2380 463 1572 3836 Fragments arranged by size: 3836 2380 1572 463 447 417 99 ApaI G_GGCC'C Cuts at: 4770 6163 4770 Size: 1393 7821 ApaLI G'TGCA_C Cuts at: 7271 8427 7271 Size: 1156 8058 ApoI r'AATT_y Cuts at: 1493 6523 6749 6901 9010 1493 Size: 5030 226 152 2109 1697 Fragments arranged by size: 5030 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 7768 160 Size: 872 6736 1606 Fragments arranged by size: 6736 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9214 AvaI C'yCGr_G Cuts at: 1870 1943 3004 3070 3635 4685 4951 5030 5435 Size: 73 1061 66 565 1050 266 79 405 Cuts at: 5435 5568 6177 6298 6409 6539 1870 Size: 133 609 121 111 130 4545 Fragments arranged by size: 4545 1061 1050 609 565 405 266 133 130 121 111 79 73 66 BamHI G'GATC_C Cuts at: 3631 6216 6544 6911 3631 Size: 2585 328 367 5934 Fragments arranged by size: 5934 2585 367 328 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3044 3661 4349 5003 5733 Size: 325 217 627 1257 617 688 654 730 Cuts at: 5733 5788 6535 7990 618 Size: 55 747 1455 1842 Fragments arranged by size: 1842 1455 1257 747 730 688 654 627 617 325 217 55 BanII G_rGCy'C Cuts at: 1391 2013 2712 3071 3934 4108 4770 5519 5575 Size: 622 699 359 863 174 662 749 56 Cuts at: 5575 6163 6176 6508 6533 1391 Size: 588 13 332 25 4072 Fragments arranged by size: 4072 863 749 699 662 622 588 359 332 174 56 25 13 BbeI G_GCGC'C Cuts at: 3665 3665 Size: 9214 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2389 3250 4063 4151 4390 4798 5309 Size: 971 490 861 813 88 239 408 511 Cuts at: 5309 5699 928 Size: 390 4443 Fragments arranged by size: 4443 971 861 813 511 490 408 390 239 88 BbvCI CC'TCA_GC Cuts at: 2014 5264 6020 2014 Size: 3250 756 5208 Fragments arranged by size: 5208 3250 756 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3074 3108 3184 3218 7405 7439 1903 Size: 34 1137 34 76 34 4187 34 3678 Fragments arranged by size: 4187 3678 1137 76 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7106 8316 7106 Size: 1210 8004 BclI T'GATC_A Cuts at: 1548 1548 Size: 9214 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2154 2757 3101 Size: 107 122 71 631 605 482 603 344 Cuts at: 3101 4172 4862 5001 7825 136 Size: 1071 690 139 2824 1525 Fragments arranged by size: 2824 1525 1071 690 631 605 603 482 344 139 122 107 71 BglII A'GATC_T Cuts at: 3417 3451 5379 9209 3417 Size: 34 1928 3830 3422 Fragments arranged by size: 3830 3422 1928 34 BlpI GC'TnA_GC Cuts at: 5673 6631 5673 Size: 958 8256 Bme1580I G_kGCm'C Cuts at: 1790 2150 2336 2741 3011 4352 4479 4770 5030 Size: 360 186 405 270 1341 127 291 260 Cuts at: 5030 5778 5793 6163 7275 8431 1790 Size: 748 15 370 1112 1156 2573 Fragments arranged by size: 2573 1341 1156 1112 748 405 370 360 291 270 260 186 127 15 BmgBI CAC'GTC Cuts at: 1318 3289 3787 6367 1318 Size: 1971 498 2580 4165 Fragments arranged by size: 4165 2580 1971 498 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2497 3367 6166 Size: 416 53 83 399 24 1080 870 2799 Cuts at: 6166 6945 7903 442 Size: 779 958 1753 Fragments arranged by size: 2799 1753 1080 958 870 779 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9214 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3830 3830 Size: 9214 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2155 2440 2939 3014 3536 4190 5363 5387 Size: 916 285 499 75 522 654 1173 24 Cuts at: 5387 5564 5903 6464 7874 1239 Size: 177 339 561 1410 2579 Fragments arranged by size: 2579 1410 1173 916 654 561 522 499 339 285 177 75 24 Bpu10I CC'TnA_GC Cuts at: 2014 2408 2684 5264 6020 2014 Size: 394 276 2580 756 5208 Fragments arranged by size: 5208 2580 756 394 276 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5315 7339 8204 8502 5315 Size: 2024 865 298 6027 Fragments arranged by size: 6027 2024 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2098 3173 7877 882 Size: 617 599 1075 4704 2219 Fragments arranged by size: 4704 2219 1075 617 599 BsaAI yAC'GTr Cuts at: 493 1204 3685 4450 5722 7005 493 Size: 711 2481 765 1272 1283 2702 Fragments arranged by size: 2702 2481 1283 1272 765 711 BsaBI GATnn'nnATC Cuts at: 2975 9208 2975 Size: 6233 2981 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2499 2643 3087 3662 Size: 53 83 186 936 966 144 444 575 Cuts at: 3662 4620 4803 5472 5628 7021 7403 8038 275 Size: 958 183 669 156 1393 382 635 1451 Fragments arranged by size: 1451 1393 966 958 936 669 635 575 444 382 186 183 156 144 83 53 BsaWI w'CCGG_w Cuts at: 3234 3816 4086 7647 8045 8319 8466 8877 3234 Size: 582 270 3561 398 274 147 411 3571 Fragments arranged by size: 3571 3561 582 411 398 274 270 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2438 2468 4400 4430 5516 Size: 30 165 30 1035 30 1932 30 1086 Cuts at: 5516 5546 5789 5819 5876 5906 1178 Size: 30 243 30 57 30 4486 Fragments arranged by size: 4486 1932 1086 1035 243 165 57 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2060 2318 2967 4013 4016 4037 4058 4292 Size: 874 258 649 1046 3 21 21 234 Cuts at: 4292 4544 5123 5234 5252 5369 5561 5675 5714 Size: 252 579 111 18 117 192 114 39 Cuts at: 5714 5729 6467 1186 Size: 15 738 3933 Fragments arranged by size: 3933 1046 874 738 649 579 258 252 234 192 117 114 111 39 21 21 18 15 3 BseYI C'CCAG_C Cuts at: 2255 2481 3709 4762 8417 8913 2255 Size: 226 1228 1053 3655 496 2556 Fragments arranged by size: 3655 2556 1228 1053 496 226 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3440 3459 3548 3821 5189 5561 5981 Size: 573 1704 19 89 273 1368 372 420 Cuts at: 5981 6380 1163 Size: 399 3997 Fragments arranged by size: 3997 1704 1368 573 420 399 372 273 89 19 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3757 5233 5856 6664 7425 8453 Size: 275 186 1756 1476 623 808 761 1028 Cuts at: 8453 1540 Size: 2301 Fragments arranged by size: 2301 1756 1476 1028 808 761 623 275 186 BsiHKAI G_wGCw'C Cuts at: 2712 4056 4455 4989 5178 6156 6234 7275 7360 Size: 1344 399 534 189 978 78 1041 85 Cuts at: 7360 8431 2712 Size: 1071 3495 Fragments arranged by size: 3495 1344 1071 1041 978 534 399 189 85 78 BsmI GAATG_Cn' Cuts at: 2222 5731 6725 6818 2222 Size: 3509 994 93 4618 Fragments arranged by size: 4618 3509 994 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5792 6320 5792 Size: 528 8686 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2150 2336 2712 2741 3011 3071 Size: 399 223 137 186 376 29 270 60 Cuts at: 3071 3934 4056 4108 4352 4455 4479 4770 4989 Size: 863 122 52 244 103 24 291 219 Cuts at: 4989 5030 5178 5519 5575 5778 5793 6156 6163 Size: 41 148 341 56 203 15 363 7 Cuts at: 6163 6176 6234 6508 6533 7275 7360 8431 1391 Size: 13 58 274 25 742 85 1071 2174 Fragments arranged by size: 2174 1071 863 742 399 376 363 341 291 274 270 244 223 219 203 186 148 137 122 103 85 60 58 56 52 41 29 25 24 15 13 7 BspEI T'CCGG_A Cuts at: 3234 8877 3234 Size: 5643 3571 BspHI T'CATG_A Cuts at: 2475 2646 7103 8833 2475 Size: 171 4457 1730 2856 Fragments arranged by size: 4457 2856 1730 171 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2644 2837 3117 4160 4664 5109 5155 5280 Size: 1800 193 280 1043 504 445 46 125 Cuts at: 5280 5493 5542 6402 6421 6569 844 Size: 213 49 860 19 148 3489 Fragments arranged by size: 3489 1800 1043 860 504 445 280 213 193 148 125 49 46 19 BsrBI CCG'CTC Cuts at: 1394 1823 7101 1394 Size: 429 5278 3507 Fragments arranged by size: 5278 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3090 3402 7709 7883 65 Size: 1183 1842 312 4307 174 1396 Fragments arranged by size: 4307 1842 1396 1183 312 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2639 3756 4307 5056 7858 8045 1700 Size: 110 829 1117 551 749 2802 187 2869 Fragments arranged by size: 2869 2802 1117 829 749 551 187 110 BsrGI T'GTAC_A Cuts at: 96 6276 96 Size: 6180 3034 BssHII G'CGCG_C Cuts at: 1925 3651 1925 Size: 1726 7488 BssSI C'ACGA_G Cuts at: 2861 3350 7274 8286 2861 Size: 489 3924 1012 3789 Fragments arranged by size: 3924 3789 1012 489 BstAPI GCAn_nnn'nTGC Cuts at: 794 3313 3619 794 Size: 2519 306 6389 Fragments arranged by size: 6389 2519 306 BstBI TT'CG_AA Cuts at: 8018 8018 Size: 9214 BstEII G'GTnAC_C Cuts at: 1184 2521 1184 Size: 1337 7877 BstYI r'GATC_y Cuts at: 1972 2970 3219 3417 3451 3631 3975 4947 5379 Size: 998 249 198 34 180 344 972 432 Cuts at: 5379 6216 6544 6911 7296 7313 8765 8851 8863 Size: 837 328 367 385 17 1452 86 12 Cuts at: 8863 9209 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 998 972 837 432 385 367 346 344 328 249 198 180 86 34 17 12 Bsu36I CC'TnA_GG Cuts at: 2021 3838 5924 2021 Size: 1817 2086 5311 Fragments arranged by size: 5311 2086 1817 BtgI C'CryG_G Cuts at: 513 1065 2004 2555 2772 2878 3657 3858 4248 Size: 552 939 551 217 106 779 201 390 Cuts at: 4248 4356 4389 5227 5475 5520 5655 5859 5868 Size: 108 33 838 248 45 135 204 9 Cuts at: 5868 5944 5985 6054 513 Size: 76 41 69 3673 Fragments arranged by size: 3673 939 838 779 552 551 390 248 217 204 201 135 108 106 76 69 45 41 33 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3934 5122 487 Size: 683 56 2708 1188 4579 Fragments arranged by size: 4579 2708 1188 683 56 BtsI GCAGTG_nn' Cuts at: 3387 3418 3443 4987 6730 7524 7544 3387 Size: 31 25 1544 1743 794 20 5057 Fragments arranged by size: 5057 1743 1544 794 31 25 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9214 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9179 DraI TTT'AAA Cuts at: 6518 6518 Size: 9214 DraIII CAC_nnn'GTG Cuts at: 2340 3685 4080 6066 2340 Size: 1345 395 1986 5488 Fragments arranged by size: 5488 1986 1345 395 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8221 809 Size: 507 6905 1802 Fragments arranged by size: 6905 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2812 2881 2961 Size: 54 1425 50 275 18 982 69 80 Cuts at: 2961 3081 3102 3297 3346 4854 5230 5310 5853 Size: 120 21 195 49 1508 376 80 543 Cuts at: 5853 6236 6333 6661 7550 9179 8 Size: 383 97 328 889 1629 43 Fragments arranged by size: 1629 1508 1425 982 889 543 383 376 328 275 195 120 97 80 80 69 54 50 49 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 5230 5853 6661 1537 Size: 275 3418 623 808 4090 Fragments arranged by size: 4090 3418 808 623 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2129 2558 3515 4092 4325 4517 4550 4643 Size: 776 429 957 577 233 192 33 93 Cuts at: 4643 4823 5111 5285 5501 6095 7144 1353 Size: 180 288 174 216 594 1049 3423 Fragments arranged by size: 3423 1049 957 776 594 577 429 288 233 216 192 180 174 93 33 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4055 5639 6088 7801 8175 8321 Size: 1064 138 2466 1584 449 1713 374 146 Cuts at: 8321 387 Size: 1280 Fragments arranged by size: 2466 1713 1584 1280 1064 449 374 146 138 EcoICRI GAG'CTC Cuts at: 2710 2710 Size: 9214 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2155 2440 2582 2662 2939 3014 3432 Size: 393 523 285 142 80 277 75 418 Cuts at: 3432 3536 3554 4001 4190 4306 4579 5321 5363 Size: 104 18 447 189 116 273 742 42 Cuts at: 5363 5387 5408 5525 5564 5596 5903 6230 6278 Size: 24 21 117 39 32 307 327 48 Cuts at: 6278 6464 7277 7874 8661 8929 1239 Size: 186 813 597 787 268 1524 Fragments arranged by size: 1524 813 787 742 597 523 447 418 393 327 307 285 277 273 268 189 186 142 117 116 104 80 75 48 42 39 32 24 21 18 EcoNI CCTnn'n_nnAGG Cuts at: 2588 4223 5216 6224 6482 2588 Size: 1635 993 1008 258 5320 Fragments arranged by size: 5320 1635 1008 993 258 EcoO109I rG'GnC_Cy Cuts at: 2344 2610 2631 2758 2874 4766 6160 6414 2344 Size: 266 21 127 116 1892 1394 254 5144 Fragments arranged by size: 5144 1892 1394 266 254 127 116 21 EcoRI G'AATT_C Cuts at: 6523 6901 6523 Size: 378 8836 EcoRV GAT'ATC Cuts at: 3229 3229 Size: 9214 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4603 4822 4603 Size: 219 8995 FseI GG_CCGG'CC Cuts at: 5060 5060 Size: 9214 FspI TGC'GCA Cuts at: 7720 7720 Size: 9214 HaeII r_GCGC'y Cuts at: 1678 3643 3665 4037 4888 5206 8361 1678 Size: 1965 22 372 851 318 3155 2531 Fragments arranged by size: 3155 2531 1965 851 372 318 22 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2438 2470 2571 2603 4899 Size: 32 135 32 866 32 101 32 2296 Cuts at: 4899 4931 5876 5908 6184 6216 7855 7887 7929 Size: 32 945 32 276 32 1639 32 42 Cuts at: 7929 7961 1373 Size: 32 2626 Fragments arranged by size: 2626 2296 1639 945 866 276 135 101 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5470 6558 6804 669 Size: 988 281 3532 1088 246 3079 Fragments arranged by size: 3532 3079 1088 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9214 HpaI GTT'AAC Cuts at: 6804 6804 Size: 9214 KasI G'GCGC_C Cuts at: 3661 3661 Size: 9214 KpnI G_GTAC'C Cuts at: 6539 6539 Size: 9214 MfeI C'AATT_G Cuts at: 6813 6813 Size: 9214 MluI A'CGCG_T Cuts at: 9090 9090 Size: 9214 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3510 8328 8512 1305 Size: 2205 4818 184 2007 Fragments arranged by size: 4818 2205 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2814 2963 3104 3299 3348 Size: 54 1425 343 982 149 141 195 49 Cuts at: 3348 4856 10 Size: 1508 4368 Fragments arranged by size: 4368 1508 1425 982 343 195 149 141 54 49 MslI CAynn'nnrTG Cuts at: 518 1166 2861 4124 4781 5141 5724 6272 7172 Size: 648 1695 1263 657 360 583 548 900 Cuts at: 7172 7531 7690 518 Size: 359 159 2042 Fragments arranged by size: 2042 1695 1263 900 657 648 583 548 360 359 159 MspA1I CmG'CkG Cuts at: 1606 2557 3247 3370 3473 3532 3659 3956 4297 Size: 951 690 123 103 59 127 297 341 Cuts at: 4297 4358 5431 5607 5657 6024 6494 6630 7307 Size: 61 1073 176 50 367 470 136 677 Cuts at: 7307 8455 8700 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1073 951 690 677 470 367 341 297 245 176 136 127 123 103 61 59 50 NaeI GCC'GGC Cuts at: 5058 5058 Size: 9214 NarI GG'CG_CC Cuts at: 3662 3662 Size: 9214 NcoI C'CATG_G Cuts at: 513 1065 2004 3858 5475 5520 5859 5868 5985 Size: 552 939 1854 1617 45 339 9 117 Cuts at: 5985 6054 513 Size: 69 3673 Fragments arranged by size: 3673 1854 1617 939 552 339 117 69 45 9 NdeI CA'TA_TG Cuts at: 387 387 Size: 9214 NgoMIV G'CCGG_C Cuts at: 5056 5056 Size: 9214 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9214 NotI GC'GGCC_GC Cuts at: 5853 6661 5853 Size: 808 8406 NspI r_CATG'y Cuts at: 1132 1229 2360 4285 6572 8117 1132 Size: 97 1131 1925 2287 1545 2229 Fragments arranged by size: 2287 2229 1925 1545 1131 97 PasI CC'CwG_GG Cuts at: 1348 5793 1348 Size: 4445 4769 PciI A'CATG_T Cuts at: 2356 8113 2356 Size: 5757 3457 PflMI CCAn_nnn'nTGG Cuts at: 1108 4230 6426 1108 Size: 3122 2196 3896 Fragments arranged by size: 3896 3122 2196 PfoI T'CCnGG_A Cuts at: 2361 3165 3261 3513 6351 2361 Size: 804 96 252 2838 5224 Fragments arranged by size: 5224 2838 804 252 96 PmeI GTTT'AAAC Cuts at: 6518 6518 Size: 9214 PmlI CAC'GTG Cuts at: 3685 5722 3685 Size: 2037 7177 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4400 4432 5789 5821 7283 7315 8796 8828 4400 Size: 32 1357 32 1462 32 1481 32 4786 Fragments arranged by size: 4786 1481 1462 1357 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2344 2631 2874 2344 Size: 287 243 8684 Fragments arranged by size: 8684 287 243 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9214 PsiI TTA'TAA Cuts at: 6784 6784 Size: 9214 PspOMI G'GGCC_C Cuts at: 4766 6159 4766 Size: 1393 7821 PstI C_TGCA'G Cuts at: 830 3615 3675 3971 4161 4341 4661 4994 5277 Size: 2785 60 296 190 180 320 333 283 Cuts at: 5277 6098 6566 830 Size: 821 468 3478 Fragments arranged by size: 3478 2785 821 468 333 320 296 283 190 180 60 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9214 PvuII CAG'CTG Cuts at: 3247 3370 3473 3532 6024 3247 Size: 123 103 59 2492 6437 Fragments arranged by size: 6437 2492 123 103 59 SacI G_AGCT'C Cuts at: 2712 2712 Size: 9214 SacII CC_GC'GG Cuts at: 2558 3660 4359 5658 2558 Size: 1102 699 1299 6114 Fragments arranged by size: 6114 1299 1102 699 SalI G'TCGA_C Cuts at: 1936 6556 1936 Size: 4620 4594 SanDI GG'GwC_CC Cuts at: 2344 2631 2874 2344 Size: 287 243 8684 Fragments arranged by size: 8684 287 243 SapI GCTCTTCn'nnn_ Cuts at: 2558 3515 4517 4550 4643 5111 5285 6095 2558 Size: 957 1002 33 93 468 174 810 5677 Fragments arranged by size: 5677 1002 957 810 468 174 93 33 SbfI CC_TGCA'GG Cuts at: 4661 6566 4661 Size: 1905 7309 ScaI AGT'ACT Cuts at: 7462 9174 7462 Size: 1712 7502 SfcI C'TryA_G Cuts at: 826 1045 2141 3611 3671 3967 4157 4337 4657 Size: 219 1096 1470 60 296 190 180 320 Cuts at: 4657 4990 5273 6094 6562 7697 8378 8569 826 Size: 333 283 821 468 1135 681 191 1471 Fragments arranged by size: 1471 1470 1135 1096 821 681 468 333 320 296 283 219 191 190 180 60 SfiI GGCCn_nnn'nGGCC Cuts at: 4862 4862 Size: 9214 SfoI GGC'GCC Cuts at: 3663 3663 Size: 9214 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9214 SgrAI Cr'CCGG_yG Cuts at: 1700 2639 1700 Size: 939 8275 SmaI CCC'GGG Cuts at: 1872 3006 4687 4953 6179 6541 1872 Size: 1134 1681 266 1226 362 4545 Fragments arranged by size: 4545 1681 1226 1134 362 266 SmlI C'TyrA_G Cuts at: 820 1017 1943 5330 7318 8219 8481 820 Size: 197 926 3387 1988 901 262 1553 Fragments arranged by size: 3387 1988 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 7005 493 Size: 6512 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9214 SphI G_CATG'C Cuts at: 1132 4285 6572 1132 Size: 3153 2287 3774 Fragments arranged by size: 3774 3153 2287 SrfI GCCC'GGGC Cuts at: 3006 3006 Size: 9214 SspI AAT'ATT Cuts at: 5 52 7138 5 Size: 47 7086 2081 Fragments arranged by size: 7086 2081 47 StuI AGG'CCT Cuts at: 1517 2583 3311 4136 4214 1517 Size: 1066 728 825 78 6517 Fragments arranged by size: 6517 1066 825 728 78 StyI C'CwwG_G Cuts at: 513 1065 2004 2815 2943 3858 3960 3993 4176 Size: 552 939 811 128 915 102 33 183 Cuts at: 4176 4224 4518 5475 5520 5859 5868 5985 6054 Size: 48 294 957 45 339 9 117 69 Cuts at: 6054 6653 513 Size: 599 3074 Fragments arranged by size: 3074 957 939 915 811 599 552 339 294 183 128 117 102 69 48 45 33 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4825 7241 7258 7411 7596 8041 325 Size: 1255 3245 2416 17 153 185 445 1498 Fragments arranged by size: 3245 2416 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2793 4590 5904 Size: 274 80 33 51 1443 816 1797 1314 Cuts at: 5904 6276 7460 9172 96 Size: 372 1184 1712 138 Fragments arranged by size: 1797 1712 1443 1314 1184 816 372 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 9174 9206 9174 Size: 32 9182 Tth111I GACn'n_nGTC Cuts at: 1316 2393 1316 Size: 1077 8137 XbaI T'CTAG_A Cuts at: 6550 6550 Size: 9214 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2983 3341 5866 2983 Size: 358 2525 6331 Fragments arranged by size: 6331 2525 358 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9214 XmaI C'CCGG_G Cuts at: 1870 3004 4685 4951 6177 6539 1870 Size: 1134 1681 266 1226 362 4545 Fragments arranged by size: 4545 1681 1226 1134 362 266 XmnI GAAnn'nnTTC Cuts at: 3509 7343 3509 Size: 3834 5380 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2500 3088 4621 4804 Size: 53 83 186 936 966 588 1533 183 Cuts at: 4804 5629 7022 276 Size: 825 1393 2468 Fragments arranged by size: 2468 1533 1393 966 936 825 588 186 183 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I FspAI NruI NsiI PacI PspXI PsrI RsrII SexAI SwaI