Restriction map for
pFN21AE2549
(Linear) (Six-base) MAPSORT of: KIEE2549.seq Check: 437 from: 1 to: 4509
KIEE2549.orf
With 182 enzymes: SgfI *
August 16, 2021 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2155 3150 3275 3537 4416 4509
Size: 2155 995 125 262 879 93
Fragments arranged by size:
2155 995 879 262 125 93
AatII G_ACGT'C
Cuts at: 0 497 1085 2618 2801 3626 4509
Size: 497 588 1533 183 825 883
Fragments arranged by size:
1533 883 825 588 497 183
AccI GT'mk_AC
Cuts at: 0 2912 4509
Size: 2912 1597
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 577 657 1427 1549 1996 2301 2574 3316
Size: 577 80 770 122 447 305 273 742
Cuts at: 3316 3403 3520 3591 4225 4273 4509
Size: 87 117 71 634 48 236
Fragments arranged by size:
770 742 634 577 447 305 273 236
122 117 87 80 71 48
AflIII A'CryG_T
Cuts at: 0 351 4509
Size: 351 4158
AleI CACnn'nnGTG
Cuts at: 0 2119 4509
Size: 2119 2390
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4012 4509
Size: 4012 497
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2395 2427 2881 2913 3784 3816 4509
Size: 2395 32 454 32 871 32 693
Fragments arranged by size:
2395 871 693 454 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1146 1245 1692 2109 4489 4509
Size: 1146 99 447 417 2380 20
Fragments arranged by size:
2380 1146 447 417 99 20
ApaI G_GGCC'C
Cuts at: 0 2765 4158 4509
Size: 2765 1393 351
Fragments arranged by size:
2765 1393 351
AvaI C'yCGr_G
Cuts at: 0 999 1065 1630 2680 2946 3025 3430 3563
Size: 999 66 565 1050 266 79 405 133
Cuts at: 3563 4172 4293 4404 4509
Size: 609 121 111 105
Fragments arranged by size:
1050 999 609 565 405 266 133 121
111 105 79 66
BamHI G'GATC_C
Cuts at: 0 1626 4211 4509
Size: 1626 2585 298
Fragments arranged by size:
2585 1626 298
BanI G'GyrC_C
Cuts at: 0 1039 1656 2344 2998 3728 3783 4509
Size: 1039 617 688 654 730 55 726
Fragments arranged by size:
1039 730 726 688 654 617 55
BanII G_rGCy'C
Cuts at: 0 8 707 1066 1929 2103 2765 3514 3570
Size: 8 699 359 863 174 662 749 56
Cuts at: 3570 4158 4171 4503 4509
Size: 588 13 332 6
Fragments arranged by size:
863 749 699 662 588 359 332 174
56 13 8 6
BbeI G_GCGC'C
Cuts at: 0 1660 4509
Size: 1660 2849
BbsI GAAGACnn'nnnn_
Cuts at: 0 384 1245 2058 2146 2385 2793 3304 3694
Size: 384 861 813 88 239 408 511 390
Cuts at: 3694 4509
Size: 815
Fragments arranged by size:
861 815 813 511 408 390 384 239
88
BbvCI CC'TCA_GC
Cuts at: 0 9 3259 4015 4509
Size: 9 3250 756 494
Fragments arranged by size:
3250 756 494 9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1069 1103 1179 1213 4509
Size: 1069 34 76 34 3296
Fragments arranged by size:
3296 1069 76 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 149 752 1096 2167 2857 2996 4509
Size: 149 603 344 1071 690 139 1513
Fragments arranged by size:
1513 1071 690 603 344 149 139
BglII A'GATC_T
Cuts at: 0 1412 1446 3374 4509
Size: 1412 34 1928 1135
Fragments arranged by size:
1928 1412 1135 34
BlpI GC'TnA_GC
Cuts at: 0 3668 4509
Size: 3668 841
Bme1580I G_kGCm'C
Cuts at: 0 145 331 736 1006 2347 2474 2765 3025
Size: 145 186 405 270 1341 127 291 260
Cuts at: 3025 3773 3788 4158 4509
Size: 748 15 370 351
Fragments arranged by size:
1341 748 405 370 351 291 270 260
186 145 127 15
BmgBI CAC'GTC
Cuts at: 0 1284 1782 4362 4509
Size: 1284 498 2580 147
Fragments arranged by size:
2580 1284 498 147
BmrI ACTGGGnnnn_n'
Cuts at: 0 492 1362 4161 4509
Size: 492 870 2799 348
Fragments arranged by size:
2799 870 492 348
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1825 4509
Size: 1825 2684
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 150 435 934 1009 1531 2185 3358 3382
Size: 150 285 499 75 522 654 1173 24
Cuts at: 3382 3559 3898 4459 4509
Size: 177 339 561 50
Fragments arranged by size:
1173 654 561 522 499 339 285 177
150 75 50 24
Bpu10I CC'TnA_GC
Cuts at: 0 9 403 679 3259 4015 4509
Size: 9 394 276 2580 756 494
Fragments arranged by size:
2580 756 494 394 276 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3310 4509
Size: 3310 1199
BsaI GGTCTCn'nnnn_
Cuts at: 0 93 1168 4509
Size: 93 1075 3341
Fragments arranged by size:
3341 1075 93
BsaAI yAC'GTr
Cuts at: 0 1680 2445 3717 4509
Size: 1680 765 1272 792
Fragments arranged by size:
1680 1272 792 765
BsaBI GATnn'nnATC
Cuts at: 0 970 4509
Size: 970 3539
BsaHI Gr'CG_yC
Cuts at: 0 494 638 1082 1657 2615 2798 3467 3623
Size: 494 144 444 575 958 183 669 156
Cuts at: 3623 4509
Size: 886
Fragments arranged by size:
958 886 669 575 494 444 183 156
144
BsaWI w'CCGG_w
Cuts at: 0 1229 1811 2081 4509
Size: 1229 582 270 2428
Fragments arranged by size:
2428 1229 582 270
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 433 463 2395 2425 3511 3541 3784 3814
Size: 433 30 1932 30 1086 30 243 30
Cuts at: 3814 3871 3901 4509
Size: 57 30 608
Fragments arranged by size:
1932 1086 608 433 243 57 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 313 962 2008 2011 2032 2053 2287
Size: 55 258 649 1046 3 21 21 234
Cuts at: 2287 2539 3118 3229 3247 3364 3556 3670 3709
Size: 252 579 111 18 117 192 114 39
Cuts at: 3709 3724 4462 4509
Size: 15 738 47
Fragments arranged by size:
1046 738 649 579 258 252 234 192
117 114 111 55 47 39 21 21
18 15 3
BseYI C'CCAG_C
Cuts at: 0 250 476 1704 2757 4509
Size: 250 226 1228 1053 1752
Fragments arranged by size:
1752 1228 1053 250 226
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1435 1454 1543 1816 3184 3556 3976 4375
Size: 1435 19 89 273 1368 372 420 399
Cuts at: 4375 4509
Size: 134
Fragments arranged by size:
1435 1368 420 399 372 273 134 89
19
BsiEI CG_ry'CG
Cuts at: 0 1752 3228 3851 4509
Size: 1752 1476 623 658
Fragments arranged by size:
1752 1476 658 623
BsiHKAI G_wGCw'C
Cuts at: 0 707 2051 2450 2984 3173 4151 4229 4509
Size: 707 1344 399 534 189 978 78 280
Fragments arranged by size:
1344 978 707 534 399 280 189 78
BsmI GAATG_Cn'
Cuts at: 0 217 3726 4509
Size: 217 3509 783
Fragments arranged by size:
3509 783 217
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3787 4315 4509
Size: 3787 528 194
Fragments arranged by size:
3787 528 194
Bsp1286I G_dGCh'C
Cuts at: 0 8 145 331 707 736 1006 1066 1929
Size: 8 137 186 376 29 270 60 863
Cuts at: 1929 2051 2103 2347 2450 2474 2765 2984 3025
Size: 122 52 244 103 24 291 219 41
Cuts at: 3025 3173 3514 3570 3773 3788 4151 4158 4171
Size: 148 341 56 203 15 363 7 13
Cuts at: 4171 4229 4503 4509
Size: 58 274 6
Fragments arranged by size:
863 376 363 341 291 274 270 244
219 203 186 148 137 122 103 60
58 56 52 41 29 24 15 13
8 7 6
BspEI T'CCGG_A
Cuts at: 0 1229 4509
Size: 1229 3280
BspHI T'CATG_A
Cuts at: 0 470 641 4509
Size: 470 171 3868
Fragments arranged by size:
3868 470 171
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 639 832 1112 2155 2659 3104 3150 3275
Size: 639 193 280 1043 504 445 46 125
Cuts at: 3275 3488 3537 4397 4416 4509
Size: 213 49 860 19 93
Fragments arranged by size:
1043 860 639 504 445 280 213 193
125 93 49 46 19
BsrDI GCAATG_nn'
Cuts at: 0 1085 1397 4509
Size: 1085 312 3112
Fragments arranged by size:
3112 1085 312
BsrFI r'CCGG_y
Cuts at: 0 634 1751 2302 3051 4509
Size: 634 1117 551 749 1458
Fragments arranged by size:
1458 1117 749 634 551
BsrGI T'GTAC_A
Cuts at: 0 4271 4509
Size: 4271 238
BssHII G'CGCG_C
Cuts at: 0 1646 4509
Size: 1646 2863
BssSI C'ACGA_G
Cuts at: 0 856 1345 4509
Size: 856 489 3164
Fragments arranged by size:
3164 856 489
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1308 1614 4509
Size: 1308 306 2895
Fragments arranged by size:
2895 1308 306
BstEII G'GTnAC_C
Cuts at: 0 516 4509
Size: 516 3993
BstYI r'GATC_y
Cuts at: 0 965 1214 1412 1446 1626 1970 2942 3374
Size: 965 249 198 34 180 344 972 432
Cuts at: 3374 4211 4509
Size: 837 298
Fragments arranged by size:
972 965 837 432 344 298 249 198
180 34
Bsu36I CC'TnA_GG
Cuts at: 0 16 1833 3919 4509
Size: 16 1817 2086 590
Fragments arranged by size:
2086 1817 590 16
BtgI C'CryG_G
Cuts at: 0 550 767 873 1652 1853 2243 2351 2384
Size: 550 217 106 779 201 390 108 33
Cuts at: 2384 3222 3470 3515 3650 3854 3863 3939 3980
Size: 838 248 45 135 204 9 76 41
Cuts at: 3980 4049 4509
Size: 69 460
Fragments arranged by size:
838 779 550 460 390 248 217 204
201 135 108 106 76 69 45 41
33 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1929 3117 4509
Size: 1929 1188 1392
Fragments arranged by size:
1929 1392 1188
BtsI GCAGTG_nn'
Cuts at: 0 1382 1413 1438 2982 4509
Size: 1382 31 25 1544 1527
Fragments arranged by size:
1544 1527 1382 31 25
DraIII CAC_nnn'GTG
Cuts at: 0 335 1680 2075 4061 4509
Size: 335 1345 395 1986 448
Fragments arranged by size:
1986 1345 448 395 335
EaeI y'GGCC_r
Cuts at: 0 807 876 956 1076 1097 1292 1341 2849
Size: 807 69 80 120 21 195 49 1508
Cuts at: 2849 3225 3305 3848 4231 4328 4509
Size: 376 80 543 383 97 181
Fragments arranged by size:
1508 807 543 383 376 195 181 120
97 80 80 69 49 21
EagI C'GGCC_G
Cuts at: 0 3225 3848 4509
Size: 3225 623 661
Fragments arranged by size:
3225 661 623
EarI CTCTTCn'nnn_
Cuts at: 0 124 553 1510 2087 2320 2512 2545 2638
Size: 124 429 957 577 233 192 33 93
Cuts at: 2638 2818 3106 3280 3496 4090 4509
Size: 180 288 174 216 594 419
Fragments arranged by size:
957 594 577 429 419 288 233 216
192 180 174 124 93 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2050 3634 4083 4509
Size: 2050 1584 449 426
Fragments arranged by size:
2050 1584 449 426
EcoICRI GAG'CTC
Cuts at: 0 705 4509
Size: 705 3804
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 150 435 577 657 934 1009 1427 1531
Size: 150 285 142 80 277 75 418 104
Cuts at: 1531 1549 1996 2185 2301 2574 3316 3358 3382
Size: 18 447 189 116 273 742 42 24
Cuts at: 3382 3403 3520 3559 3591 3898 4225 4273 4459
Size: 21 117 39 32 307 327 48 186
Cuts at: 4459 4509
Size: 50
Fragments arranged by size:
742 447 418 327 307 285 277 273
189 186 150 142 117 116 104 80
75 50 48 42 39 32 24 21
18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 583 2218 3211 4219 4477 4509
Size: 583 1635 993 1008 258 32
Fragments arranged by size:
1635 1008 993 583 258 32
EcoO109I rG'GnC_Cy
Cuts at: 0 339 605 626 753 869 2761 4155 4409
Size: 339 266 21 127 116 1892 1394 254
Cuts at: 4409 4509
Size: 100
Fragments arranged by size:
1892 1394 339 266 254 127 116 100
21
EcoRV GAT'ATC
Cuts at: 0 1224 4509
Size: 1224 3285
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2598 2817 4509
Size: 2598 219 1692
Fragments arranged by size:
2598 1692 219
FseI GG_CCGG'CC
Cuts at: 0 3055 4509
Size: 3055 1454
HaeII r_GCGC'y
Cuts at: 0 1638 1660 2032 2883 3201 4509
Size: 1638 22 372 851 318 1308
Fragments arranged by size:
1638 1308 851 372 318 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 433 465 566 598 2894 2926 3871 3903
Size: 433 32 101 32 2296 32 945 32
Cuts at: 3903 4179 4211 4509
Size: 276 32 298
Fragments arranged by size:
2296 945 433 298 276 101 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 3465 4509
Size: 3465 1044
KasI G'GCGC_C
Cuts at: 0 1656 4509
Size: 1656 2853
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1505 4509
Size: 1505 3004
MscI TGG'CCA
Cuts at: 0 809 958 1099 1294 1343 2851 4509
Size: 809 149 141 195 49 1508 1658
Fragments arranged by size:
1658 1508 809 195 149 141 49
MslI CAynn'nnrTG
Cuts at: 0 856 2119 2776 3136 3719 4267 4509
Size: 856 1263 657 360 583 548 242
Fragments arranged by size:
1263 856 657 583 548 360 242
MspA1I CmG'CkG
Cuts at: 0 552 1242 1365 1468 1527 1654 1951 2292
Size: 552 690 123 103 59 127 297 341
Cuts at: 2292 2353 3426 3602 3652 4019 4489 4509
Size: 61 1073 176 50 367 470 20
Fragments arranged by size:
1073 690 552 470 367 341 297 176
127 123 103 61 59 50 20
NaeI GCC'GGC
Cuts at: 0 3053 4509
Size: 3053 1456
NarI GG'CG_CC
Cuts at: 0 1657 4509
Size: 1657 2852
NcoI C'CATG_G
Cuts at: 0 1853 3470 3515 3854 3863 3980 4049 4509
Size: 1853 1617 45 339 9 117 69 460
Fragments arranged by size:
1853 1617 460 339 117 69 45 9
NgoMIV G'CCGG_C
Cuts at: 0 3051 4509
Size: 3051 1458
NotI GC'GGCC_GC
Cuts at: 0 3848 4509
Size: 3848 661
NspI r_CATG'y
Cuts at: 0 355 2280 4509
Size: 355 1925 2229
Fragments arranged by size:
2229 1925 355
PasI CC'CwG_GG
Cuts at: 0 3788 4509
Size: 3788 721
PciI A'CATG_T
Cuts at: 0 351 4509
Size: 351 4158
PflMI CCAn_nnn'nTGG
Cuts at: 0 2225 4421 4509
Size: 2225 2196 88
Fragments arranged by size:
2225 2196 88
PfoI T'CCnGG_A
Cuts at: 0 356 1160 1256 1508 4346 4509
Size: 356 804 96 252 2838 163
Fragments arranged by size:
2838 804 356 252 163 96
PmlI CAC'GTG
Cuts at: 0 1680 3717 4509
Size: 1680 2037 792
Fragments arranged by size:
2037 1680 792
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2395 2427 3784 3816 4509
Size: 2395 32 1357 32 693
Fragments arranged by size:
2395 1357 693 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 339 626 869 4509
Size: 339 287 243 3640
Fragments arranged by size:
3640 339 287 243
PspOMI G'GGCC_C
Cuts at: 0 2761 4154 4509
Size: 2761 1393 355
Fragments arranged by size:
2761 1393 355
PstI C_TGCA'G
Cuts at: 0 1610 1670 1966 2156 2336 2656 2989 3272
Size: 1610 60 296 190 180 320 333 283
Cuts at: 3272 4093 4509
Size: 821 416
Fragments arranged by size:
1610 821 416 333 320 296 283 190
180 60
PvuII CAG'CTG
Cuts at: 0 1242 1365 1468 1527 4019 4509
Size: 1242 123 103 59 2492 490
Fragments arranged by size:
2492 1242 490 123 103 59
SacI G_AGCT'C
Cuts at: 0 707 4509
Size: 707 3802
SacII CC_GC'GG
Cuts at: 0 553 1655 2354 3653 4509
Size: 553 1102 699 1299 856
Fragments arranged by size:
1299 1102 856 699 553
SanDI GG'GwC_CC
Cuts at: 0 339 626 869 4509
Size: 339 287 243 3640
Fragments arranged by size:
3640 339 287 243
SapI GCTCTTCn'nnn_
Cuts at: 0 553 1510 2512 2545 2638 3106 3280 4090
Size: 553 957 1002 33 93 468 174 810
Cuts at: 4090 4509
Size: 419
Fragments arranged by size:
1002 957 810 553 468 419 174 93
33
SbfI CC_TGCA'GG
Cuts at: 0 2656 4509
Size: 2656 1853
SfcI C'TryA_G
Cuts at: 0 136 1606 1666 1962 2152 2332 2652 2985
Size: 136 1470 60 296 190 180 320 333
Cuts at: 2985 3268 4089 4509
Size: 283 821 420
Fragments arranged by size:
1470 821 420 333 320 296 283 190
180 136 60
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2857 4509
Size: 2857 1652
SfoI GGC'GCC
Cuts at: 0 1658 4509
Size: 1658 2851
SgrAI Cr'CCGG_yG
Cuts at: 0 634 4509
Size: 634 3875
SmaI CCC'GGG
Cuts at: 0 1001 2682 2948 4174 4509
Size: 1001 1681 266 1226 335
Fragments arranged by size:
1681 1226 1001 335 266
SmlI C'TyrA_G
Cuts at: 0 3325 4509
Size: 3325 1184
SphI G_CATG'C
Cuts at: 0 2280 4509
Size: 2280 2229
SrfI GCCC'GGGC
Cuts at: 0 1001 4509
Size: 1001 3508
StuI AGG'CCT
Cuts at: 0 578 1306 2131 2209 4509
Size: 578 728 825 78 2300
Fragments arranged by size:
2300 825 728 578 78
StyI C'CwwG_G
Cuts at: 0 810 938 1853 1955 1988 2171 2219 2513
Size: 810 128 915 102 33 183 48 294
Cuts at: 2513 3470 3515 3854 3863 3980 4049 4509
Size: 957 45 339 9 117 69 460
Fragments arranged by size:
957 915 810 460 339 294 183 128
117 102 69 48 45 33 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2820 4509
Size: 2820 1689
TatI w'GTAC_w
Cuts at: 0 788 2585 3899 4271 4509
Size: 788 1797 1314 372 238
Fragments arranged by size:
1797 1314 788 372 238
Tth111I GACn'n_nGTC
Cuts at: 0 388 4509
Size: 388 4121
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 978 1336 3861 4509
Size: 978 358 2525 648
Fragments arranged by size:
2525 978 648 358
XmaI C'CCGG_G
Cuts at: 0 999 2680 2946 4172 4509
Size: 999 1681 266 1226 337
Fragments arranged by size:
1681 1226 999 337 266
XmnI GAAnn'nnTTC
Cuts at: 0 1504 4509
Size: 1504 3005
ZraI GAC'GTC
Cuts at: 0 495 1083 2616 2799 3624 4509
Size: 495 588 1533 183 825 885
Fragments arranged by size:
1533 885 825 588 495 183
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AleI AlfI AloI
AlwNI ApaI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstEII BstYI Bsu36I BtgI BtgZI
BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV FalI FseI HaeII Hin4I HincII
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PasI PciI PflMI PfoI PmlI
PpiI PpuMI PspOMI PstI PvuII SacI SacII SanDI
SapI SbfI SfcI SfiI SfoI SgrAI SmaI SmlI
SphI SrfI StuI StyI TaqII TatI Tth111I XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BciVI BclI BfrBI
BmtI BsiWI BsrBI BstBI BstXI BstZ17I ClaI CspCI
DraI DrdI EcoRI FspI FspAI HindIII HpaI KpnI
MfeI MluI NdeI NheI NruI NsiI PacI PmeI
PshAI PsiI PspXI PsrI PvuI RsrII SalI ScaI
SexAI SgfI SnaBI SpeI SspI SwaI TstI XbaI
XhoI