(Circular) (Six-base) MAPSORT of: pFN21AE3356.seq Check: 9719 from: 1 to: 7849 pFN21AE3356 7849 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3727 3727 Size: 7849 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5659 278 Size: 53 83 186 936 4123 2468 Fragments arranged by size: 4123 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4258 5192 1937 Size: 2321 934 4594 Fragments arranged by size: 4594 2321 934 Acc65I G'GTAC_C Cuts at: 2738 5170 2738 Size: 2432 5417 AclI AA'CG_TT Cuts at: 1563 5976 6349 1563 Size: 4413 373 3063 Fragments arranged by size: 4413 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3348 3618 3651 4404 4478 4784 5912 7296 Size: 1716 270 33 753 74 306 1128 1384 Cuts at: 7296 7564 1632 Size: 268 1917 Fragments arranged by size: 1917 1716 1384 1128 753 306 270 268 74 33 AfeI AGC'GCT Cuts at: 3772 3772 Size: 7849 AflII C'TTAA_G Cuts at: 820 1017 3731 820 Size: 197 2714 4938 Fragments arranged by size: 4938 2714 197 AflIII A'CryG_T Cuts at: 1203 2251 2481 3260 6748 7725 1203 Size: 1048 230 779 3488 977 1327 Fragments arranged by size: 3488 1327 1048 977 779 230 AgeI A'CCGG_T Cuts at: 6680 6680 Size: 7849 AhdI GACnn_n'nnGTC Cuts at: 6578 6578 Size: 7849 AleI CACnn'nnGTG Cuts at: 1166 5000 1166 Size: 3834 4015 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4039 1184 Size: 2855 4994 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2192 2224 2338 2370 2192 Size: 32 114 32 7671 Fragments arranged by size: 7671 114 32 32 AlwNI CAG_nnn'CTG Cuts at: 4195 4900 5592 7164 4195 Size: 705 692 1572 4880 Fragments arranged by size: 4880 1572 705 692 ApaLI G'TGCA_C Cuts at: 2618 2714 5906 7062 2618 Size: 96 3192 1156 3405 Fragments arranged by size: 3405 3192 1156 96 ApoI r'AATT_y Cuts at: 1493 2384 3509 3670 4066 4736 5104 5158 5384 Size: 891 1125 161 396 670 368 54 226 Cuts at: 5384 5536 7645 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1125 891 670 396 368 226 161 152 54 AseI AT'TA_AT Cuts at: 160 1032 6403 160 Size: 872 5371 1606 Fragments arranged by size: 5371 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7849 AvaI C'yCGr_G Cuts at: 1870 1943 2785 4574 4799 5174 1870 Size: 73 842 1789 225 375 4545 Fragments arranged by size: 4545 1789 842 375 225 73 BamHI G'GATC_C Cuts at: 3094 5179 5546 3094 Size: 2085 367 5397 Fragments arranged by size: 5397 2085 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2738 3692 4681 5170 6625 Size: 325 217 627 951 954 989 489 1455 Cuts at: 6625 618 Size: 1842 Fragments arranged by size: 1842 1455 989 954 951 627 489 325 217 BanII G_rGCy'C Cuts at: 1391 4084 4391 4749 4928 5168 1391 Size: 2693 307 358 179 240 4072 Fragments arranged by size: 4072 2693 358 307 240 179 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2244 3336 3542 928 Size: 971 345 1092 206 5235 Fragments arranged by size: 5235 1092 971 345 206 BbvCI CC'TCA_GC Cuts at: 2868 2868 Size: 7849 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3018 3052 3575 3609 5010 Size: 34 50 34 997 34 523 34 1401 Cuts at: 5010 5044 6040 6074 1903 Size: 34 996 34 3678 Fragments arranged by size: 3678 1401 997 996 523 50 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4896 5741 6951 4896 Size: 845 1210 5794 Fragments arranged by size: 5794 1210 845 BclI T'GATC_A Cuts at: 1548 1548 Size: 7849 BfrBI ATG'CAT Cuts at: 2862 2862 Size: 7849 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6460 136 Size: 107 122 71 631 605 4788 1525 Fragments arranged by size: 4788 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7844 7844 Size: 7849 BlpI GC'TnA_GC Cuts at: 5266 5266 Size: 7849 Bme1580I G_kGCm'C Cuts at: 1790 2622 2718 4684 5910 7066 1790 Size: 832 96 1966 1226 1156 2573 Fragments arranged by size: 2573 1966 1226 1156 832 96 BmgBI CAC'GTC Cuts at: 1318 3261 1318 Size: 1943 5906 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3102 5580 6538 Size: 416 53 83 399 24 1685 2478 958 Cuts at: 6538 442 Size: 1753 Fragments arranged by size: 2478 1753 1685 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7849 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2653 2653 Size: 7849 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3169 4217 4912 6509 1239 Size: 1930 1048 695 1597 2579 Fragments arranged by size: 2579 1930 1597 1048 695 Bpu10I CC'TnA_GC Cuts at: 2868 4922 2868 Size: 2054 5795 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2836 5974 6839 7137 2836 Size: 3138 865 298 3548 Fragments arranged by size: 3548 3138 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2812 4004 6512 882 Size: 617 1313 1192 2508 2219 Fragments arranged by size: 2508 2219 1313 1192 617 BsaAI yAC'GTr Cuts at: 493 1204 5640 493 Size: 711 4436 2702 Fragments arranged by size: 4436 2702 711 BsaBI GATnn'nnATC Cuts at: 7843 7843 Size: 7849 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5656 6038 6673 275 Size: 53 83 186 936 4123 382 635 1451 Fragments arranged by size: 4123 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3204 3955 4265 4555 6282 6680 6954 7101 7512 Size: 751 310 290 1727 398 274 147 411 Cuts at: 7512 3204 Size: 3541 Fragments arranged by size: 3541 1727 751 411 398 310 290 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2338 2368 2495 2525 2746 Size: 30 165 30 935 30 127 30 221 Cuts at: 2746 2776 3250 3280 3937 3967 1178 Size: 30 474 30 657 30 5060 Fragments arranged by size: 5060 935 657 474 221 165 127 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2629 3939 3942 1186 Size: 1443 1310 3 5093 Fragments arranged by size: 5093 1443 1310 3 BseYI C'CCAG_C Cuts at: 4285 7052 7548 4285 Size: 2767 496 4586 Fragments arranged by size: 4586 2767 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3048 4536 1163 Size: 573 1312 1488 4476 Fragments arranged by size: 4476 1488 1312 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2628 5299 6060 7088 1540 Size: 275 186 627 2671 761 1028 2301 Fragments arranged by size: 2671 2301 1028 761 627 275 186 BsiHKAI G_wGCw'C Cuts at: 2622 2706 2718 2969 4928 5910 5995 7066 2622 Size: 84 12 251 1959 982 85 1071 3405 Fragments arranged by size: 3405 1959 1071 982 251 85 84 12 BsmI GAATG_Cn' Cuts at: 2781 5122 5360 5453 2781 Size: 2341 238 93 5177 Fragments arranged by size: 5177 2341 238 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2622 2706 2718 2969 4084 4391 4684 Size: 399 832 84 12 251 1115 307 293 Cuts at: 4684 4749 4928 5168 5910 5995 7066 1391 Size: 65 179 240 742 85 1071 2174 Fragments arranged by size: 2174 1115 1071 832 742 399 307 293 251 240 179 85 84 65 12 BspEI T'CCGG_A Cuts at: 3955 7512 3955 Size: 3557 4292 BspHI T'CATG_A Cuts at: 5738 7468 5738 Size: 1730 6119 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3727 5204 844 Size: 2883 1477 3489 Fragments arranged by size: 3489 2883 1477 BsrBI CCG'CTC Cuts at: 1394 1823 5736 1394 Size: 429 3913 3507 Fragments arranged by size: 3913 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6344 6518 65 Size: 1183 5096 174 1396 Fragments arranged by size: 5096 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2268 3212 6493 6680 1700 Size: 110 458 944 3281 187 2869 Fragments arranged by size: 3281 2869 944 458 187 110 BsrGI T'GTAC_A Cuts at: 96 2052 96 Size: 1956 5893 BssHII G'CGCG_C Cuts at: 1925 2748 1925 Size: 823 7026 BssSI C'ACGA_G Cuts at: 5909 6921 5909 Size: 1012 6837 BstAPI GCAn_nnn'nTGC Cuts at: 794 2211 4900 794 Size: 1417 2689 3743 Fragments arranged by size: 3743 2689 1417 BstBI TT'CG_AA Cuts at: 6653 6653 Size: 7849 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7849 BstXI CCAn_nnnn'nTGG Cuts at: 3238 3238 Size: 7849 BstYI r'GATC_y Cuts at: 1972 2436 3094 3335 5179 5546 5931 5948 7400 Size: 464 658 241 1844 367 385 17 1452 Cuts at: 7400 7486 7498 7844 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1844 1452 658 464 385 367 346 241 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3067 3084 3303 4074 4676 513 Size: 552 2002 17 219 771 602 3686 Fragments arranged by size: 3686 2002 771 602 552 219 17 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7110 Fragments arranged by size: 7110 683 56 BtsI GCAGTG_nn' Cuts at: 3130 5365 6159 6179 3130 Size: 2235 794 20 4800 Fragments arranged by size: 4800 2235 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7849 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7814 DraI TTT'AAA Cuts at: 4065 5153 4065 Size: 1088 6761 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6856 809 Size: 507 5540 1802 Fragments arranged by size: 5540 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2625 5296 6185 Size: 54 1425 50 275 18 795 2671 889 Cuts at: 6185 7814 8 Size: 1629 43 Fragments arranged by size: 2671 1629 1425 889 795 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2625 5296 1537 Size: 275 813 2671 4090 Fragments arranged by size: 4090 2671 813 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4089 4599 4687 4810 5779 1353 Size: 2736 510 88 123 969 3423 Fragments arranged by size: 3423 2736 969 510 123 88 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3953 6436 6810 6956 387 Size: 1064 138 2364 2483 374 146 1280 Fragments arranged by size: 2483 2364 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4926 4926 Size: 7849 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3169 3348 3618 3651 4217 4404 4478 Size: 393 1537 179 270 33 566 187 74 Cuts at: 4478 4784 4912 5912 6509 7296 7564 1239 Size: 306 128 1000 597 787 268 1524 Fragments arranged by size: 1537 1524 1000 787 597 566 393 306 270 268 187 179 128 74 33 EcoNI CCTnn'n_nnAGG Cuts at: 4398 4398 Size: 7849 EcoO109I rG'GnC_Cy Cuts at: 4408 4408 Size: 7849 EcoRI G'AATT_C Cuts at: 5158 5536 5158 Size: 378 7471 FspI TGC'GCA Cuts at: 3660 6355 3660 Size: 2695 5154 HaeII r_GCGC'y Cuts at: 1678 3080 3774 4901 6996 1678 Size: 1402 694 1127 2095 2531 Fragments arranged by size: 2531 2095 1402 1127 694 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2102 2134 2746 2778 3250 Size: 32 135 32 530 32 612 32 472 Cuts at: 3250 3282 4150 4182 6490 6522 6564 6596 1373 Size: 32 868 32 2308 32 42 32 2626 Fragments arranged by size: 2626 2308 868 612 530 472 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4259 4792 5193 5439 669 Size: 988 281 2321 533 401 246 3079 Fragments arranged by size: 3079 2321 988 533 401 281 246 HindIII A'AGCT_T Cuts at: 748 3150 748 Size: 2402 5447 HpaI GTT'AAC Cuts at: 5439 5439 Size: 7849 KpnI G_GTAC'C Cuts at: 2742 5174 2742 Size: 2432 5417 MfeI C'AATT_G Cuts at: 4090 5448 4090 Size: 1358 6491 MluI A'CGCG_T Cuts at: 7725 7725 Size: 7849 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6963 7147 1305 Size: 5658 184 2007 Fragments arranged by size: 5658 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6027 Fragments arranged by size: 6027 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3236 3486 3502 3816 5000 5807 6166 Size: 648 2070 250 16 314 1184 807 359 Cuts at: 6166 6325 518 Size: 159 2042 Fragments arranged by size: 2070 2042 1184 807 648 359 314 250 159 16 MspA1I CmG'CkG Cuts at: 1606 2135 2905 3172 3650 3824 4019 5265 5942 Size: 529 770 267 478 174 195 1246 677 Cuts at: 5942 7090 7335 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1246 1148 770 677 529 478 267 245 195 174 NaeI GCC'GGC Cuts at: 3214 3214 Size: 7849 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7297 NdeI CA'TA_TG Cuts at: 387 4842 387 Size: 4455 3394 NgoMIV G'CCGG_C Cuts at: 3212 3212 Size: 7849 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7849 NotI GC'GGCC_GC Cuts at: 5296 5296 Size: 7849 NsiI A_TGCA'T Cuts at: 2864 2864 Size: 7849 NspI r_CATG'y Cuts at: 1132 1229 2032 2209 2255 2485 2530 5207 6752 Size: 97 803 177 46 230 45 2677 1545 Cuts at: 6752 1132 Size: 2229 Fragments arranged by size: 2677 2229 1545 803 230 177 97 46 45 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7849 PciI A'CATG_T Cuts at: 2251 2481 6748 2251 Size: 230 4267 3352 Fragments arranged by size: 4267 3352 230 PflMI CCAn_nnn'nTGG Cuts at: 1108 3172 4195 1108 Size: 2064 1023 4762 Fragments arranged by size: 4762 2064 1023 PfoI T'CCnGG_A Cuts at: 4687 4687 Size: 7849 PmeI GTTT'AAAC Cuts at: 5153 5153 Size: 7849 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2338 2370 5918 5950 7431 7463 2338 Size: 32 3548 32 1481 32 2724 Fragments arranged by size: 3548 2724 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 4765 1613 Size: 3152 4697 PsiI TTA'TAA Cuts at: 5419 5419 Size: 7849 PspXI vC'TCGA_Gb Cuts at: 2785 2785 Size: 7849 PstI C_TGCA'G Cuts at: 830 2216 2571 2805 3529 4028 4732 5201 830 Size: 1386 355 234 724 499 704 469 3478 Fragments arranged by size: 3478 1386 724 704 499 469 355 234 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7849 PvuII CAG'CTG Cuts at: 2905 3172 3824 4019 2905 Size: 267 652 195 6735 Fragments arranged by size: 6735 652 267 195 SacI G_AGCT'C Cuts at: 4928 4928 Size: 7849 SalI G'TCGA_C Cuts at: 1936 4257 5191 1936 Size: 2321 934 4594 Fragments arranged by size: 4594 2321 934 SapI GCTCTTCn'nnn_ Cuts at: 4089 4089 Size: 7849 SbfI CC_TGCA'GG Cuts at: 5201 5201 Size: 7849 ScaI AGT'ACT Cuts at: 6097 7809 6097 Size: 1712 6137 SfcI C'TryA_G Cuts at: 826 1045 2212 2567 2801 3525 3925 4024 4728 Size: 219 1167 355 234 724 400 99 704 Cuts at: 4728 5197 6332 7013 7204 826 Size: 469 1135 681 191 1471 Fragments arranged by size: 1471 1167 1135 724 704 681 469 400 355 234 219 191 99 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7849 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7849 SmaI CCC'GGG Cuts at: 1872 4801 5176 1872 Size: 2929 375 4545 Fragments arranged by size: 4545 2929 375 SmlI C'TyrA_G Cuts at: 820 1017 1943 2785 2815 3731 5953 6854 7116 Size: 197 926 842 30 916 2222 901 262 Cuts at: 7116 820 Size: 1553 Fragments arranged by size: 2222 1553 926 916 901 842 262 197 30 SnaBI TAC'GTA Cuts at: 493 5640 493 Size: 5147 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7849 SphI G_CATG'C Cuts at: 1132 5207 1132 Size: 4075 3774 SspI AAT'ATT Cuts at: 5 52 3466 5773 5 Size: 47 3414 2307 2081 Fragments arranged by size: 3414 2307 2081 47 StuI AGG'CCT Cuts at: 1517 3033 5050 1517 Size: 1516 2017 4316 Fragments arranged by size: 4316 2017 1516 StyI C'CwwG_G Cuts at: 513 1065 2196 2358 2507 3933 4492 4913 5288 Size: 552 1131 162 149 1426 559 421 375 Cuts at: 5288 513 Size: 3074 Fragments arranged by size: 3074 1426 1131 559 552 421 375 162 149 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3986 5876 5893 6046 6231 6676 325 Size: 1255 2406 1890 17 153 185 445 1498 Fragments arranged by size: 2406 1890 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2052 6095 7807 Size: 274 80 33 51 1443 75 4043 1712 Cuts at: 7807 96 Size: 138 Fragments arranged by size: 4043 1712 1443 274 138 80 75 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4281 4313 7809 7841 4281 Size: 32 3496 32 4289 Fragments arranged by size: 4289 3496 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7849 XbaI T'CTAG_A Cuts at: 5185 5185 Size: 7849 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3235 4840 3235 Size: 1605 6244 XhoI C'TCGA_G Cuts at: 1943 2785 1943 Size: 842 7007 XmaI C'CCGG_G Cuts at: 1870 4799 5174 1870 Size: 2929 375 4545 Fragments arranged by size: 4545 2929 375 XmnI GAAnn'nnTTC Cuts at: 5978 5978 Size: 7849 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5657 276 Size: 53 83 186 936 4123 2468 Fragments arranged by size: 4123 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI