(Circular) (Six-base) MAPSORT of: pFN21AE5738.seq Check: 6729 from: 1 to: 6814 pFN21AE5738 6814 bp With 182 enzymes: SgfI * December 8, 2014 13:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4624 278 Size: 53 83 186 936 3088 2468 Fragments arranged by size: 3088 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4157 1937 Size: 2220 4594 Acc65I G'GTAC_C Cuts at: 4135 4135 Size: 6814 AclI AA'CG_TT Cuts at: 1563 4113 4941 5314 1563 Size: 2550 828 373 3063 Fragments arranged by size: 3063 2550 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2834 3927 4877 6261 6529 1632 Size: 1202 1093 950 1384 268 1917 Fragments arranged by size: 1917 1384 1202 1093 950 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6617 AflIII A'CryG_T Cuts at: 1203 2763 5713 6690 1203 Size: 1560 2950 977 1327 Fragments arranged by size: 2950 1560 1327 977 AgeI A'CCGG_T Cuts at: 2724 5645 2724 Size: 2921 3893 AhdI GACnn_n'nnGTC Cuts at: 5543 5543 Size: 6814 AleI CACnn'nnGTG Cuts at: 1166 3089 1166 Size: 1923 4891 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6814 AlwNI CAG_nnn'CTG Cuts at: 4557 6129 4557 Size: 1572 5242 ApaLI G'TGCA_C Cuts at: 4871 6027 4871 Size: 1156 5658 ApoI r'AATT_y Cuts at: 1493 2861 3052 3240 3863 4123 4349 4501 6610 Size: 1368 191 188 623 260 226 152 2109 Cuts at: 6610 1493 Size: 1697 Fragments arranged by size: 2109 1697 1368 623 260 226 191 188 152 AseI AT'TA_AT Cuts at: 160 1032 5368 160 Size: 872 4336 1606 Fragments arranged by size: 4336 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6814 AvaI C'yCGr_G Cuts at: 1870 1943 2577 4139 1870 Size: 73 634 1562 4545 Fragments arranged by size: 4545 1562 634 73 BamHI G'GATC_C Cuts at: 4144 4511 4144 Size: 367 6447 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4135 5590 618 Size: 325 217 627 2348 1455 1842 Fragments arranged by size: 2348 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4133 1391 Size: 2742 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2234 928 Size: 971 335 5508 Fragments arranged by size: 5508 971 335 BbvCI CC'TCA_GC Cuts at: 3062 3374 3062 Size: 312 6502 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5005 5039 1903 Size: 34 50 34 2984 34 3678 Fragments arranged by size: 3678 2984 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3584 4706 5916 3584 Size: 1122 1210 4482 Fragments arranged by size: 4482 1210 1122 BclI T'GATC_A Cuts at: 1548 1548 Size: 6814 BfrBI ATG'CAT Cuts at: 3070 3224 3070 Size: 154 6660 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5425 136 Size: 107 122 71 631 605 3753 1525 Fragments arranged by size: 3753 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3784 6809 3784 Size: 3025 3789 BlpI GC'TnA_GC Cuts at: 4231 4231 Size: 6814 Bme1580I G_kGCm'C Cuts at: 1790 4875 6031 1790 Size: 3085 1156 2573 Fragments arranged by size: 3085 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6814 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2047 3323 3573 Size: 416 53 83 399 24 630 1276 250 Cuts at: 3573 4545 5503 442 Size: 972 958 1753 Fragments arranged by size: 1753 1276 972 958 630 416 399 250 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6814 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3796 3796 Size: 6814 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3534 3666 3943 5474 1239 Size: 2295 132 277 1531 2579 Fragments arranged by size: 2579 2295 1531 277 132 Bpu10I CC'TnA_GC Cuts at: 3062 3374 3062 Size: 312 6502 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4939 5804 6102 4939 Size: 865 298 5651 Fragments arranged by size: 5651 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3954 5477 882 Size: 617 2455 1523 2219 Fragments arranged by size: 2455 2219 1523 617 BsaAI yAC'GTr Cuts at: 493 1204 2764 3628 4605 493 Size: 711 1560 864 977 2702 Fragments arranged by size: 2702 1560 977 864 711 BsaBI GATnn'nnATC Cuts at: 3572 6808 3572 Size: 3236 3578 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4621 5003 5638 275 Size: 53 83 186 936 3088 382 635 1451 Fragments arranged by size: 3088 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2724 5247 5645 5919 6066 6477 2724 Size: 2523 398 274 147 411 3061 Fragments arranged by size: 3061 2523 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6589 Fragments arranged by size: 6589 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2096 3249 4034 1186 Size: 910 1153 785 3966 Fragments arranged by size: 3966 1153 910 785 BseYI C'CCAG_C Cuts at: 2702 2990 6017 6513 2702 Size: 288 3027 496 3003 Fragments arranged by size: 3027 3003 496 288 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2667 1163 Size: 573 931 5310 Fragments arranged by size: 5310 931 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4264 5025 6053 1540 Size: 275 186 2263 761 1028 2301 Fragments arranged by size: 2301 2263 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3627 4875 4960 6031 3627 Size: 1248 85 1071 4410 Fragments arranged by size: 4410 1248 1071 85 BsmI GAATG_Cn' Cuts at: 2427 3563 4325 4418 2427 Size: 1136 762 93 4823 Fragments arranged by size: 4823 1136 762 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3627 4133 4875 4960 6031 1391 Size: 399 1837 506 742 85 1071 2174 Fragments arranged by size: 2174 1837 1071 742 506 399 85 BspEI T'CCGG_A Cuts at: 6477 6477 Size: 6814 BspHI T'CATG_A Cuts at: 2437 4703 6433 2437 Size: 2266 1730 2818 Fragments arranged by size: 2818 2266 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4169 844 Size: 3325 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4701 1394 Size: 429 2878 3507 Fragments arranged by size: 3507 2878 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2292 2752 2985 3320 5309 5483 65 Size: 1183 1044 460 233 335 1989 174 1396 Fragments arranged by size: 1989 1396 1183 1044 460 335 233 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2724 5458 5645 1700 Size: 110 914 2734 187 2869 Fragments arranged by size: 2869 2734 914 187 110 BsrGI T'GTAC_A Cuts at: 96 2901 96 Size: 2805 4009 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6814 BssSI C'ACGA_G Cuts at: 4874 5886 4874 Size: 1012 5802 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6814 BstBI TT'CG_AA Cuts at: 5618 5618 Size: 6814 BstEII G'GTnAC_C Cuts at: 1184 2399 1184 Size: 1215 5599 BstYI r'GATC_y Cuts at: 1972 2471 3784 4144 4511 4896 4913 6365 6451 Size: 499 1313 360 367 385 17 1452 86 Cuts at: 6451 6463 6809 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1313 499 385 367 360 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2772 3474 513 Size: 552 1707 702 3853 Fragments arranged by size: 3853 1707 702 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6075 Fragments arranged by size: 6075 683 56 BtsI GCAGTG_nn' Cuts at: 2283 2425 2877 3162 3979 4330 5124 5144 2283 Size: 142 452 285 817 351 794 20 3953 Fragments arranged by size: 3953 817 794 452 351 285 142 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6814 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6779 DraI TTT'AAA Cuts at: 3907 4118 3907 Size: 211 6603 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5821 809 Size: 507 4505 1802 Fragments arranged by size: 4505 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4261 5150 6779 Size: 54 1425 50 275 18 2431 889 1629 Cuts at: 6779 8 Size: 43 Fragments arranged by size: 2431 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4261 1537 Size: 275 2449 4090 Fragments arranged by size: 4090 2449 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2266 2810 3480 4011 4744 1353 Size: 913 544 670 531 733 3423 Fragments arranged by size: 3423 913 733 670 544 531 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5401 5775 5921 387 Size: 1064 138 3812 374 146 1280 Fragments arranged by size: 3812 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2834 3534 3666 3927 3943 4877 5474 Size: 393 1202 700 132 261 16 934 597 Cuts at: 5474 6261 6529 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1202 934 787 700 597 393 268 261 132 16 EcoNI CCTnn'n_nnAGG Cuts at: 3043 3043 Size: 6814 EcoRI G'AATT_C Cuts at: 4123 4501 4123 Size: 378 6436 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3867 3867 Size: 6814 FspI TGC'GCA Cuts at: 5320 5320 Size: 6814 HaeII r_GCGC'y Cuts at: 1678 5961 1678 Size: 4283 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2560 2592 3009 3041 3434 Size: 32 135 32 988 32 417 32 393 Cuts at: 3434 3466 3577 3609 5455 5487 5529 5561 1373 Size: 32 111 32 1846 32 42 32 2626 Fragments arranged by size: 2626 1846 988 417 393 135 111 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3664 4158 4404 669 Size: 988 281 1726 494 246 3079 Fragments arranged by size: 3079 1726 988 494 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6814 HpaI GTT'AAC Cuts at: 4404 4404 Size: 6814 KpnI G_GTAC'C Cuts at: 4139 4139 Size: 6814 MfeI C'AATT_G Cuts at: 4413 4413 Size: 6814 MluI A'CGCG_T Cuts at: 6690 6690 Size: 6814 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2107 5928 6112 1305 Size: 802 3821 184 2007 Fragments arranged by size: 3821 2007 802 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4992 Fragments arranged by size: 4992 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2514 2940 3075 3086 3089 3566 4772 Size: 648 1348 426 135 11 3 477 1206 Cuts at: 4772 5131 5290 518 Size: 359 159 2042 Fragments arranged by size: 2042 1348 1206 648 477 426 359 159 135 11 3 MspA1I CmG'CkG Cuts at: 1606 3169 3554 3703 4230 4907 6055 6300 1606 Size: 1563 385 149 527 677 1148 245 2120 Fragments arranged by size: 2120 1563 1148 677 527 385 245 149 NcoI C'CATG_G Cuts at: 513 1065 3474 513 Size: 552 2409 3853 Fragments arranged by size: 3853 2409 552 NdeI CA'TA_TG Cuts at: 387 2808 387 Size: 2421 4393 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6814 NotI GC'GGCC_GC Cuts at: 4261 4261 Size: 6814 NsiI A_TGCA'T Cuts at: 3072 3226 3072 Size: 154 6660 NspI r_CATG'y Cuts at: 1132 1229 2418 2897 3827 4172 5717 1132 Size: 97 1189 479 930 345 1545 2229 Fragments arranged by size: 2229 1545 1189 930 479 345 97 PasI CC'CwG_GG Cuts at: 1348 2038 3537 1348 Size: 690 1499 4625 Fragments arranged by size: 4625 1499 690 PciI A'CATG_T Cuts at: 5713 5713 Size: 6814 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6814 PmeI GTTT'AAAC Cuts at: 4118 4118 Size: 6814 PmlI CAC'GTG Cuts at: 2764 3628 2764 Size: 864 5950 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2733 2765 4883 4915 6396 6428 2733 Size: 32 2118 32 1481 32 3119 Fragments arranged by size: 3119 2118 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6814 PsiI TTA'TAA Cuts at: 4384 4384 Size: 6814 PstI C_TGCA'G Cuts at: 830 4166 830 Size: 3336 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6814 PvuII CAG'CTG Cuts at: 3169 3554 3703 3169 Size: 385 149 6280 Fragments arranged by size: 6280 385 149 SalI G'TCGA_C Cuts at: 1936 4156 1936 Size: 2220 4594 SapI GCTCTTCn'nnn_ Cuts at: 2266 2266 Size: 6814 SbfI CC_TGCA'GG Cuts at: 4166 4166 Size: 6814 ScaI AGT'ACT Cuts at: 5062 6774 5062 Size: 1712 5102 SfcI C'TryA_G Cuts at: 826 1045 4162 5297 5978 6169 826 Size: 219 3117 1135 681 191 1471 Fragments arranged by size: 3117 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6814 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6814 SmaI CCC'GGG Cuts at: 1872 4141 1872 Size: 2269 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2577 4918 5819 6081 820 Size: 197 926 634 2341 901 262 1553 Fragments arranged by size: 2341 1553 926 901 634 262 197 SnaBI TAC'GTA Cuts at: 493 4605 493 Size: 4112 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6814 SphI G_CATG'C Cuts at: 1132 2418 3827 4172 1132 Size: 1286 1409 345 3774 Fragments arranged by size: 3774 1409 1286 345 SspI AAT'ATT Cuts at: 5 52 2607 2656 4738 5 Size: 47 2555 49 2082 2081 Fragments arranged by size: 2555 2082 2081 49 47 StuI AGG'CCT Cuts at: 1517 3919 3956 1517 Size: 2402 37 4375 Fragments arranged by size: 4375 2402 37 StyI C'CwwG_G Cuts at: 513 1065 3474 4253 513 Size: 552 2409 779 3074 Fragments arranged by size: 3074 2409 779 552 SwaI ATTT'AAAT Cuts at: 3907 3907 Size: 6814 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2030 2457 3453 4841 4858 5011 5196 Size: 1255 450 427 996 1388 17 153 185 Cuts at: 5196 5641 325 Size: 445 1498 Fragments arranged by size: 1498 1388 1255 996 450 445 427 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2901 5060 6772 Size: 274 80 33 51 1443 924 2159 1712 Cuts at: 6772 96 Size: 138 Fragments arranged by size: 2159 1712 1443 924 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3141 3173 6774 6806 3141 Size: 32 3601 32 3149 Fragments arranged by size: 3601 3149 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6814 XbaI T'CTAG_A Cuts at: 4150 4150 Size: 6814 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2312 4076 2312 Size: 1764 5050 XhoI C'TCGA_G Cuts at: 1943 2577 1943 Size: 634 6180 XmaI C'CCGG_G Cuts at: 1870 4139 1870 Size: 2269 4545 XmnI GAAnn'nnTTC Cuts at: 2070 4943 2070 Size: 2873 3941 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4622 276 Size: 53 83 186 936 3088 2468 Fragments arranged by size: 3088 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI