################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BpuAI BsaHI BsaI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmFI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HindIII HpaI KasI KpnI Ksp632I KspI MfeI MluI MroI MspA1I MunI MvnI NarI NciI NdeI NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SapI SbfI ScaI SexAI SfiI SfoI SfuI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 18 GTCGAC GT^[AC][GT]AC AcsI 589 AAATTC [GA]^AATT[CT] AlwNI 61 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 589 AAATTC [GA]^AATT[CT] AspI 118 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 455 GGACC G^G[AT]CC BalI 561 TGGCCA TGG^CCA BfaI 503 CTAG C^TAG BlpI 66 GCTGAGC GC^T[ACGT]AGC BmyI 700 GGGCAC G[AGT]GC[ACT]^C BpmI 101 CTGGAG CTGGAG(16/14) Bpu1102I 66 GCTGAGC GC^T[ACGT]AGC BsaAI 412 TACGTG [CT]AC^GT[GA] BsaJI 564 CCATGG C^C[ACGT][ACGT]GG BsaWI 395 TCCGGT [AT]^CCGG[AT] BsmBI 104 GAGACG CGTCTC(1/5) Bsp1286I 700 GGGCAC G[AGT]GC[ACT]^C Bsp1407I 1201 TGTACA T^GTACA BsrFI 27 GCCGGC [GA]^CCGG[CT] BsrGI 1201 TGTACA T^GTACA BstAPI 1119 GCAAAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 66 GCTGAGC GC^T[ACGT]AGC Cfr10I 27 GCCGGC [GA]^CCGG[CT] Cfr13I 455 GGACC G^G[ACGT]CC DsaI 564 CCATGG C^C[GA][CT]GG EcoT14I 564 CCATGG C^C[AT][AT]GG FauI 31 GCGGG CCCGC(4/6) HaeII 1304 GGCGCT [GA]GCGC^[CT] HgaI 118 GACGC GACGC(5/10) HincII 18 GTCGAC GT[CT]^[GA]AC HindII 18 GTCGAC GT[CT]^[GA]AC HphI 742 GGTGA GGTGA(8/7) MaeI 503 CTAG C^TAG MaeII 413 ACGT A^CGT MluNI 561 TGGCCA TGG^CCA MscI 561 TGGCCA TGG^CCA NaeI 27 GCCGGC GCC^GGC NcoI 564 CCATGG C^CATGG NgoMIV 27 GCCGGC G^CCGGC NspI 41 ACATGC [GA]CATG^[CT] PflFI 118 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 1212 GACTC GAGTC(4/5) SalI 18 GTCGAC G^TCGAC Sau96I 455 GGACC G^G[ACGT]CC SgrAI 26 CGCCGGCG C[GA]^CCGG[CT]G SmlI 262 CTCAAG C^T[CT][GA]AG SspBI 1201 TGTACA T^GTACA StyI 564 CCATGG C^C[AT][AT]GG Tth111I 118 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 502 TCTAGA T^CTAGA XcmI 279 CCAACCATTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AciI 31 GCGG CCGC(-3/-1) 49 GCGG BbvI 1265 GCAGC GCAGC(8/12) 1231 GCTGC BsiYI 283 CCATTGTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1295 CCAGTATTTGG BslI 283 CCATTGTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1295 CCAGTATTTGG BsmI 813 GAATGC GAATGC(1/-1) 1268 GCATTC BssKI 187 CCTGG ^CC[ACGT]GG 997 CCTGG BstNI 187 CCTGG CC^[AT]GG 997 CCTGG CfoI 25 GCGC GCG^C 1305 GCGC EaeI 50 CGGCCA [CT]^GGCC[GA] 561 TGGCCA EcoRII 187 CCTGG ^CC[AT]GG 997 CCTGG HapII 28 CCGG C^CGG 396 CCGG HhaI 25 GCGC GCG^C 1305 GCGC HinP1I 25 GCGC G^CGC 1305 GCGC HpaII 28 CCGG C^CGG 396 CCGG MspI 28 CCGG C^CGG 396 CCGG MvaI 187 CCTGG CC^[AT]GG 997 CCTGG PflMI 283 CCATTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1295 CCAGTATTTGG ScrFI 187 CCTGG CC^[ACGT]GG 997 CCTGG SfcI 344 CTGTAG C^T[GA][CT]AG 491 CTGTAG SspI 236 AATATT AAT^ATT 681 AATATT TfiI 826 GAATC G^A[AT]TC 1039 GAATC TseI 1231 GCTGC G^C[AT]GC 1265 GCAGC Van91I 283 CCATTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1295 CCAGTATTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AfaI AluI AlwI BsaBI BsmAI BsrI BstF5I BstYI Cac8I DdeI DpnII Fnu4HI FokI HaeIII HinfI ItaI MaeIII MamI MboI MboII MflI MnlI MseI MslI MwoI NdeII NlaIII RsaI Sau3AI SfaNI TaqI Tru9I Tsp45I Tsp509I TspRI TthHB8I XhoII