(Circular) (Six-base) MAPSORT of: pFN21AE2517.seq Check: 2753 from: 1 to: 6028 pFN21AE2517 6028 bp With 182 enzymes: SgfI * February 25, 2014 17:19 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3838 278 Size: 53 83 186 936 2302 2468 Fragments arranged by size: 2468 2302 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2024 3371 1937 Size: 87 1347 4594 Fragments arranged by size: 4594 1347 87 Acc65I G'GTAC_C Cuts at: 3349 3349 Size: 6028 AclI AA'CG_TT Cuts at: 1563 4155 4528 1563 Size: 2592 373 3063 Fragments arranged by size: 3063 2592 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4091 5475 5743 1632 Size: 2459 1384 268 1917 Fragments arranged by size: 2459 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5831 AflIII A'CryG_T Cuts at: 1203 4927 5904 1203 Size: 3724 977 1327 Fragments arranged by size: 3724 1327 977 AgeI A'CCGG_T Cuts at: 4859 4859 Size: 6028 AhdI GACnn_n'nnGTC Cuts at: 4757 4757 Size: 6028 AleI CACnn'nnGTG Cuts at: 1166 2678 1166 Size: 1512 4516 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6028 AlwNI CAG_nnn'CTG Cuts at: 2071 3771 5343 2071 Size: 1700 1572 2756 Fragments arranged by size: 2756 1700 1572 ApaLI G'TGCA_C Cuts at: 4085 5241 4085 Size: 1156 4872 ApoI r'AATT_y Cuts at: 1493 2594 3337 3563 3715 5824 1493 Size: 1101 743 226 152 2109 1697 Fragments arranged by size: 2109 1697 1101 743 226 152 AseI AT'TA_AT Cuts at: 160 1032 4582 160 Size: 872 3550 1606 Fragments arranged by size: 3550 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6028 AvaI C'yCGr_G Cuts at: 1870 1943 3353 1870 Size: 73 1410 4545 Fragments arranged by size: 4545 1410 73 BamHI G'GATC_C Cuts at: 3358 3725 3358 Size: 367 5661 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3349 4804 618 Size: 325 217 627 1562 1455 1842 Fragments arranged by size: 1842 1562 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3347 1391 Size: 1956 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5057 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4219 4253 1903 Size: 34 2282 34 3678 Fragments arranged by size: 3678 2282 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3920 5130 3920 Size: 1210 4818 BclI T'GATC_A Cuts at: 1548 1548 Size: 6028 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4639 136 Size: 107 122 71 631 605 2967 1525 Fragments arranged by size: 2967 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6023 6023 Size: 6028 BlpI GC'TnA_GC Cuts at: 2072 3445 2072 Size: 1373 4655 Bme1580I G_kGCm'C Cuts at: 1790 2709 4089 5245 1790 Size: 919 1380 1156 2573 Fragments arranged by size: 2573 1380 1156 919 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6028 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3033 3759 4717 Size: 416 53 83 399 24 1616 726 958 Cuts at: 4717 442 Size: 1753 Fragments arranged by size: 1753 1616 958 726 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6028 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2502 2502 Size: 6028 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2127 4688 1239 Size: 888 2561 2579 Fragments arranged by size: 2579 2561 888 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2252 4153 5018 5316 2252 Size: 1901 865 298 2964 Fragments arranged by size: 2964 1901 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4691 882 Size: 617 3192 2219 Fragments arranged by size: 3192 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2419 3819 493 Size: 711 1215 1400 2702 Fragments arranged by size: 2702 1400 1215 711 BsaBI GATnn'nnATC Cuts at: 2613 2736 2844 6022 2613 Size: 123 108 3178 2619 Fragments arranged by size: 3178 2619 123 108 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3835 4217 4852 275 Size: 53 83 186 936 2302 382 635 1451 Fragments arranged by size: 2302 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2400 4461 4859 5133 5280 5691 2400 Size: 2061 398 274 147 411 2737 Fragments arranged by size: 2737 2061 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5803 Fragments arranged by size: 5803 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6028 BseYI C'CCAG_C Cuts at: 3312 5231 5727 3312 Size: 1919 496 3613 Fragments arranged by size: 3613 1919 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5455 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3478 4239 5267 1540 Size: 275 186 1477 761 1028 2301 Fragments arranged by size: 2301 1477 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4089 4174 5245 4089 Size: 85 1071 4872 Fragments arranged by size: 4872 1071 85 BsmI GAATG_Cn' Cuts at: 2824 3273 3539 3632 2824 Size: 449 266 93 5220 Fragments arranged by size: 5220 449 266 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2103 2103 Size: 6028 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2709 3347 4089 4174 5245 1391 Size: 399 919 638 742 85 1071 2174 Fragments arranged by size: 2174 1071 919 742 638 399 85 BspEI T'CCGG_A Cuts at: 5691 5691 Size: 6028 BspHI T'CATG_A Cuts at: 3917 5647 3917 Size: 1730 4298 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3383 844 Size: 2539 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3915 1394 Size: 429 2092 3507 Fragments arranged by size: 3507 2092 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4523 4697 65 Size: 1183 3275 174 1396 Fragments arranged by size: 3275 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2032 4672 4859 1700 Size: 110 222 2640 187 2869 Fragments arranged by size: 2869 2640 222 187 110 BsrGI T'GTAC_A Cuts at: 96 3206 96 Size: 3110 2918 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6028 BssSI C'ACGA_G Cuts at: 4088 5100 4088 Size: 1012 5016 BstAPI GCAn_nnn'nTGC Cuts at: 794 3130 794 Size: 2336 3692 BstBI TT'CG_AA Cuts at: 4832 4832 Size: 6028 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6028 BstYI r'GATC_y Cuts at: 1972 2329 2504 2608 3187 3358 3725 4110 4127 Size: 357 175 104 579 171 367 385 17 Cuts at: 4127 5579 5665 5677 6023 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 579 385 367 357 346 175 171 104 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2569 513 Size: 552 1504 3972 Fragments arranged by size: 3972 1504 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2956 487 Size: 683 56 1730 3559 Fragments arranged by size: 3559 1730 683 56 BtsI GCAGTG_nn' Cuts at: 2083 2552 3544 4338 4358 2083 Size: 469 992 794 20 3753 Fragments arranged by size: 3753 992 794 469 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6028 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5993 DraI TTT'AAA Cuts at: 3332 3332 Size: 6028 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5035 809 Size: 507 3719 1802 Fragments arranged by size: 3719 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2566 3475 Size: 54 1425 50 275 18 225 511 909 Cuts at: 3475 4364 5993 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 909 889 511 275 225 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3475 1537 Size: 275 1663 4090 Fragments arranged by size: 4090 1663 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3958 1353 Size: 2605 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4615 4989 5135 387 Size: 1064 138 3026 374 146 1280 Fragments arranged by size: 3026 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2127 4091 4688 5475 5743 1239 Size: 393 495 1964 597 787 268 1524 Fragments arranged by size: 1964 1524 787 597 495 393 268 EcoRI G'AATT_C Cuts at: 3337 3715 3337 Size: 378 5650 FspI TGC'GCA Cuts at: 4534 4534 Size: 6028 HaeII r_GCGC'y Cuts at: 1678 3313 5175 1678 Size: 1635 1862 2531 Fragments arranged by size: 2531 1862 1635 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2515 2547 2659 2691 4669 Size: 32 135 32 943 32 112 32 1978 Cuts at: 4669 4701 4743 4775 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1978 943 135 112 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2025 3372 3618 669 Size: 988 281 87 1347 246 3079 Fragments arranged by size: 3079 1347 988 281 246 87 HindIII A'AGCT_T Cuts at: 748 748 Size: 6028 HpaI GTT'AAC Cuts at: 3618 3618 Size: 6028 KpnI G_GTAC'C Cuts at: 3353 3353 Size: 6028 MfeI C'AATT_G Cuts at: 3627 3627 Size: 6028 MluI A'CGCG_T Cuts at: 5904 5904 Size: 6028 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5142 5326 1305 Size: 3837 184 2007 Fragments arranged by size: 3837 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2568 10 Size: 54 1425 343 736 3470 Fragments arranged by size: 3470 1425 736 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2574 2678 2712 3228 3986 4345 4504 Size: 648 1408 104 34 516 758 359 159 Cuts at: 4504 518 Size: 2042 Fragments arranged by size: 2042 1408 758 648 516 359 159 104 34 MspA1I CmG'CkG Cuts at: 1606 3444 4121 5269 5514 1606 Size: 1838 677 1148 245 2120 Fragments arranged by size: 2120 1838 1148 677 245 NaeI GCC'GGC Cuts at: 2034 2034 Size: 6028 NcoI C'CATG_G Cuts at: 513 1065 2569 513 Size: 552 1504 3972 Fragments arranged by size: 3972 1504 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6028 NgoMIV G'CCGG_C Cuts at: 2032 2032 Size: 6028 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6028 NotI GC'GGCC_GC Cuts at: 3475 3475 Size: 6028 NspI r_CATG'y Cuts at: 1132 1229 2050 3386 4931 1132 Size: 97 821 1336 1545 2229 Fragments arranged by size: 2229 1545 1336 821 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6028 PciI A'CATG_T Cuts at: 4927 4927 Size: 6028 PflMI CCAn_nnn'nTGG Cuts at: 1108 2294 3306 1108 Size: 1186 1012 3830 Fragments arranged by size: 3830 1186 1012 PmeI GTTT'AAAC Cuts at: 3332 3332 Size: 6028 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2703 2735 4097 4129 5610 5642 2703 Size: 32 1362 32 1481 32 3089 Fragments arranged by size: 3089 1481 1362 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6028 PsiI TTA'TAA Cuts at: 2844 3598 2844 Size: 754 5274 PstI C_TGCA'G Cuts at: 830 3380 830 Size: 2550 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6028 SalI G'TCGA_C Cuts at: 1936 2023 3370 1936 Size: 87 1347 4594 Fragments arranged by size: 4594 1347 87 SbfI CC_TGCA'GG Cuts at: 3380 3380 Size: 6028 ScaI AGT'ACT Cuts at: 4276 5988 4276 Size: 1712 4316 SfcI C'TryA_G Cuts at: 826 1045 2349 2496 3376 4511 5192 5383 826 Size: 219 1304 147 880 1135 681 191 1471 Fragments arranged by size: 1471 1304 1135 880 681 219 191 147 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6028 SgrAI Cr'CCGG_yG Cuts at: 1700 2032 1700 Size: 332 5696 SmaI CCC'GGG Cuts at: 1872 3355 1872 Size: 1483 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2267 4132 5033 5295 820 Size: 197 926 324 1865 901 262 1553 Fragments arranged by size: 1865 1553 926 901 324 262 197 SnaBI TAC'GTA Cuts at: 493 3819 493 Size: 3326 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6028 SphI G_CATG'C Cuts at: 1132 3386 1132 Size: 2254 3774 SspI AAT'ATT Cuts at: 5 52 2243 2688 3952 5 Size: 47 2191 445 1264 2081 Fragments arranged by size: 2191 2081 1264 445 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6028 StyI C'CwwG_G Cuts at: 513 1065 2569 3467 513 Size: 552 1504 898 3074 Fragments arranged by size: 3074 1504 898 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2735 4055 4072 4225 4410 4855 325 Size: 1255 1155 1320 17 153 185 445 1498 Fragments arranged by size: 1498 1320 1255 1155 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3206 4274 5986 Size: 274 80 33 51 1443 1229 1068 1712 Cuts at: 5986 96 Size: 138 Fragments arranged by size: 1712 1443 1229 1068 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5988 6020 5988 Size: 32 5996 Tth111I GACn'n_nGTC Cuts at: 1316 2126 1316 Size: 810 5218 XbaI T'CTAG_A Cuts at: 2507 3364 2507 Size: 857 5171 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2291 2291 Size: 6028 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6028 XmaI C'CCGG_G Cuts at: 1870 3353 1870 Size: 1483 4545 XmnI GAAnn'nnTTC Cuts at: 4157 4157 Size: 6028 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3836 276 Size: 53 83 186 936 2302 2468 Fragments arranged by size: 2468 2302 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI