(Linear) (Six-base) MAPSORT of: KIEE2517.seq Check: 2726 from: 1 to: 1323 KIEE2517.orf With 182 enzymes: SgfI * February 25, 2014 15:45 .. AccI GT'mk_AC Cuts at: 0 19 1323 Size: 19 1304 AleI CACnn'nnGTG Cuts at: 0 673 1323 Size: 673 650 AlwNI CAG_nnn'CTG Cuts at: 0 66 1323 Size: 66 1257 ApoI r'AATT_y Cuts at: 0 589 1323 Size: 589 734 BlpI GC'TnA_GC Cuts at: 0 67 1323 Size: 67 1256 Bme1580I G_kGCm'C Cuts at: 0 704 1323 Size: 704 619 BmrI ACTGGGnnnn_n' Cuts at: 0 1028 1323 Size: 1028 295 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 497 1323 Size: 497 826 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 122 1323 Size: 122 1201 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 247 1323 Size: 247 1076 BsaAI yAC'GTr Cuts at: 0 414 1323 Size: 414 909 BsaBI GATnn'nnATC Cuts at: 0 608 731 839 1323 Size: 608 123 108 484 Fragments arranged by size: 608 484 123 108 BsaWI w'CCGG_w Cuts at: 0 395 1323 Size: 395 928 BseYI C'CCAG_C Cuts at: 0 1307 1323 Size: 1307 16 BsmI GAATG_Cn' Cuts at: 0 819 1268 1323 Size: 819 449 55 Fragments arranged by size: 819 449 55 BsmBI CGTCTCn'nnnn_ Cuts at: 0 98 1323 Size: 98 1225 Bsp1286I G_dGCh'C Cuts at: 0 704 1323 Size: 704 619 BsrFI r'CCGG_y Cuts at: 0 27 1323 Size: 27 1296 BsrGI T'GTAC_A Cuts at: 0 1201 1323 Size: 1201 122 BstAPI GCAn_nnn'nTGC Cuts at: 0 1125 1323 Size: 1125 198 BstYI r'GATC_y Cuts at: 0 324 499 603 1182 1323 Size: 324 175 104 579 141 Fragments arranged by size: 579 324 175 141 104 BtgI C'CryG_G Cuts at: 0 564 1323 Size: 564 759 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 951 1323 Size: 951 372 BtsI GCAGTG_nn' Cuts at: 0 78 547 1323 Size: 78 469 776 Fragments arranged by size: 776 469 78 EaeI y'GGCC_r Cuts at: 0 50 561 1323 Size: 50 511 762 Fragments arranged by size: 762 511 50 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 122 1323 Size: 122 1201 HaeII r_GCGC'y Cuts at: 0 1308 1323 Size: 1308 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 510 542 654 686 1323 Size: 510 32 112 32 637 Fragments arranged by size: 637 510 112 32 32 HincII GTy'rAC Cuts at: 0 20 1323 Size: 20 1303 MscI TGG'CCA Cuts at: 0 563 1323 Size: 563 760 MslI CAynn'nnrTG Cuts at: 0 569 673 707 1223 1323 Size: 569 104 34 516 100 Fragments arranged by size: 569 516 104 100 34 NaeI GCC'GGC Cuts at: 0 29 1323 Size: 29 1294 NcoI C'CATG_G Cuts at: 0 564 1323 Size: 564 759 NgoMIV G'CCGG_C Cuts at: 0 27 1323 Size: 27 1296 NspI r_CATG'y Cuts at: 0 45 1323 Size: 45 1278 PflMI CCAn_nnn'nTGG Cuts at: 0 289 1301 1323 Size: 289 1012 22 Fragments arranged by size: 1012 289 22 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 698 730 1323 Size: 698 32 593 Fragments arranged by size: 698 593 32 PsiI TTA'TAA Cuts at: 0 839 1323 Size: 839 484 SalI G'TCGA_C Cuts at: 0 18 1323 Size: 18 1305 SfcI C'TryA_G Cuts at: 0 344 491 1323 Size: 344 147 832 Fragments arranged by size: 832 344 147 SgrAI Cr'CCGG_yG Cuts at: 0 27 1323 Size: 27 1296 SmlI C'TyrA_G Cuts at: 0 262 1323 Size: 262 1061 SspI AAT'ATT Cuts at: 0 238 683 1323 Size: 238 445 640 Fragments arranged by size: 640 445 238 StyI C'CwwG_G Cuts at: 0 564 1323 Size: 564 759 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 730 1323 Size: 730 593 TatI w'GTAC_w Cuts at: 0 1201 1323 Size: 1201 122 Tth111I GACn'n_nGTC Cuts at: 0 121 1323 Size: 121 1202 XbaI T'CTAG_A Cuts at: 0 502 1323 Size: 502 821 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 286 1323 Size: 286 1037 Enzymes that do cut: AccI AleI AlwNI ApoI BlpI Bme1580I BmrI BplI BpmI BpuEI BsaAI BsaBI BsaWI BseYI BsmI BsmBI Bsp1286I BsrFI BsrGI BstAPI BstYI BtgI BtgZI BtsI EaeI Eco57MI HaeII Hin4I HincII MscI MslI NaeI NcoI NgoMIV NspI PflMI PpiI PsiI SalI SfcI SgrAI SmlI SspI StyI TaqII TatI Tth111I XbaI XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BmgBI BmtI Bpu10I BsaI BsaHI BsaXI BseRI BsgI BsiEI BsiHKAI BsiWI BspEI BspHI BspMI BsrBI BsrDI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI MspA1I NarI NdeI NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SmaI SnaBI SpeI SphI SrfI StuI SwaI TstI XhoI XmaI XmnI ZraI