################################## # NO CUT ENZYMES ( 26 enzymes) # ################################## AscI AseI AsnI BsiWI BssHII BstBI CpoI FseI MfeI MunI NaeI NgoMIV NheI NotI NruI NspV PacI PmeI PshBI PvuI RsrII SfuI SgrAI SnaBI SpeI SwaI ################################### # ONE CUT ENZYMES ( 16 enzymes) # ################################### AclI 2446 AACGTT AA^CGTT AgeI 661 ACCGGT A^CCGGT BsaBI 13803 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BspLU11I 13705 ACATGT A^CATGT DrdI 8761 GACATCCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC HindIII 8981 AAGCTT A^AGCTT HpaI 13116 GTTAAC GTT^AAC MamI 13803 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 10715 ACGCGT A^CGCGT NdeI 7513 CATATG CA^TATG PinAI 661 ACCGGT A^CCGGT Psp1406I 2446 AACGTT AA^CGTT SalI 7087 GTCGAC G^TCGAC SfiI 424 GGCCCCACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SspI 12455 AATATT AAT^ATT XbaI 14073 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccIII 6881 TCCGGA T^CCGGA 10334 TCCGGA AhdI 2535 GACTGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 12211 GACCTGGTGTC AspEI 2535 GACTGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 12211 GACCTGGTGTC AvrII 3112 CCTAGG C^CTAGG 11802 CCTAGG BbeI 2077 GGCGCC GGCGC^C 6109 GGCGCC BlnI 3112 CCTAGG C^CTAGG 11802 CCTAGG BseAI 6881 TCCGGA T^CCGGA 10334 TCCGGA BsiEI 7655 CGGCCG CG[GA][CT]^CG 12894 CGGCCG BspDI 9765 ATCGAT AT^CGAT 10324 ATCGAT BspEI 6881 TCCGGA T^CCGGA 10334 TCCGGA Bst1107I 7693 GTATAC GTA^TAC 15144 GTATAC BstZ17I 7693 GTATAC GTA^TAC 15144 GTATAC ClaI 9765 ATCGAT AT^CGAT 10324 ATCGAT EagI 7655 CGGCCG C^GGCCG 12894 CGGCCG Eam1105I 2535 GACTGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 12211 GACCTGGTGTC EclXI 7655 CGGCCG C^GGCCG 12894 CGGCCG Eco52I 7655 CGGCCG C^GGCCG 12894 CGGCCG EcoRI 8845 GAATTC G^AATTC 12124 GAATTC KasI 2077 GGCGCC G^GCGCC 6109 GGCGCC MroI 6881 TCCGGA T^CCGGA 10334 TCCGGA NarI 2077 GGCGCC GG^CGCC 6109 GGCGCC PaeR7I 5866 CTCGAG C^TCGAG 13036 CTCGAG SbfI 519 CCTGCAGG CCTGCA^GG 6392 CCTGCAGG SfoI 2077 GGCGCC GGC^GCC 6109 GGCGCC Sse8387I 519 CCTGCAGG CCTGCA^GG 6392 CCTGCAGG XhoI 5866 CTCGAG C^TCGAG 13036 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 190 enzymes) # ######################################### AatII Acc65I AccI AccII AciI AcsI AcyI AfaI AflII AflIII AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI Asp700I Asp718I AspHI AspI AvaI AvaII AviII BalI BamHI BanI BanII BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssKI BssSI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KpnI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PflFI PflMI PleI PmaCI PmlI PpuMI PshAI PstI PvuII RcaI RsaI SacI SacII SapI Sau3AI Sau96I ScaI ScrFI SexAI SfaNI SfcI SmaI SmlI SphI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmaCI XmaI XmnI