(Circular) (Six-base) MAPSORT of: pFN21AE3555.seq Check: 6064 from: 1 to: 20326 pFN21AE3555 20326 bp With 182 enzymes: SgfI * April 17, 2013 11:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 6057 7976 8405 11922 16197 6057 Size: 1919 429 3517 4275 10186 Fragments arranged by size: 10186 4275 3517 1919 429 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8318 9039 11472 11655 Size: 53 83 186 936 6782 721 2433 183 Cuts at: 11655 18136 278 Size: 6481 2468 Fragments arranged by size: 6782 6481 2468 2433 936 721 186 183 83 53 AccI GT'mk_AC Cuts at: 1937 7545 7677 9093 9699 14214 16689 17150 17669 Size: 5608 132 1416 606 4515 2475 461 519 Cuts at: 17669 1937 Size: 4594 Fragments arranged by size: 5608 4594 4515 2475 1416 606 519 461 132 Acc65I G'GTAC_C Cuts at: 3799 7266 9323 14982 17647 3799 Size: 3467 2057 5659 2665 6478 Fragments arranged by size: 6478 5659 3467 2665 2057 AclI AA'CG_TT Cuts at: 1563 4452 18453 18826 1563 Size: 2889 14001 373 3063 Fragments arranged by size: 14001 3063 2889 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2189 2727 2997 3033 3601 4211 4764 5030 Size: 557 538 270 36 568 610 553 266 Cuts at: 5030 5170 5507 6103 6347 6442 6581 7816 8322 Size: 140 337 596 244 95 139 1235 506 Cuts at: 8322 10307 10421 10595 10948 11888 13280 13649 14517 Size: 1985 114 174 353 940 1392 369 868 Cuts at: 14517 15095 15477 15887 18389 19773 20041 1632 Size: 578 382 410 2502 1384 268 1917 Fragments arranged by size: 2502 1985 1917 1392 1384 1235 940 868 610 596 578 568 557 553 538 506 410 382 369 353 337 270 268 266 244 174 140 139 114 95 36 AfeI AGC'GCT Cuts at: 8343 9932 17456 8343 Size: 1589 7524 11213 Fragments arranged by size: 11213 7524 1589 AflII C'TTAA_G Cuts at: 820 1017 4589 5966 7228 14311 14618 820 Size: 197 3572 1377 1262 7083 307 6528 Fragments arranged by size: 7083 6528 3572 1377 1262 307 197 AflIII A'CryG_T Cuts at: 1203 3793 8351 8353 8547 12720 15710 17190 19225 Size: 2590 4558 2 194 4173 2990 1480 2035 Cuts at: 19225 20202 1203 Size: 977 1327 Fragments arranged by size: 4558 4173 2990 2590 2035 1480 1327 977 194 2 AgeI A'CCGG_T Cuts at: 2666 19157 2666 Size: 16491 3835 AhdI GACnn_n'nnGTC Cuts at: 4545 14221 19055 4545 Size: 9676 4834 5816 Fragments arranged by size: 9676 5816 4834 AleI CACnn'nnGTG Cuts at: 1166 3727 6818 11991 12043 16940 1166 Size: 2561 3091 5173 52 4897 4552 Fragments arranged by size: 5173 4897 4552 3091 2561 52 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4149 6272 7048 10008 10956 11127 13615 13625 Size: 2965 2123 776 2960 948 171 2488 10 Cuts at: 13625 1184 Size: 7885 Fragments arranged by size: 7885 2965 2960 2488 2123 948 776 171 10 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2102 2134 2102 Size: 32 20294 AlwNI CAG_nnn'CTG Cuts at: 2105 2167 2355 3056 6265 6517 7570 7924 8459 Size: 62 188 701 3209 252 1053 354 535 Cuts at: 8459 9064 9538 9805 11167 13843 14073 14950 15805 Size: 605 474 267 1362 2676 230 877 855 Cuts at: 15805 17248 17275 17597 18069 19641 2105 Size: 1443 27 322 472 1572 2790 Fragments arranged by size: 3209 2790 2676 1572 1443 1362 1053 877 855 701 605 535 474 472 354 322 267 252 230 188 62 27 ApaI G_GGCC'C Cuts at: 2186 10370 10669 2186 Size: 8184 299 11843 Fragments arranged by size: 11843 8184 299 ApaLI G'TGCA_C Cuts at: 5977 7750 9356 13445 14048 18383 19539 5977 Size: 1773 1606 4089 603 4335 1156 6764 Fragments arranged by size: 6764 4335 4089 1773 1606 1156 603 ApoI r'AATT_y Cuts at: 1493 3224 5075 6142 8199 9296 10850 11997 14129 Size: 1731 1851 1067 2057 1097 1554 1147 2132 Cuts at: 14129 15209 15991 17520 17635 17861 18013 20122 1493 Size: 1080 782 1529 115 226 152 2109 1697 Fragments arranged by size: 2132 2109 2057 1851 1731 1697 1554 1529 1147 1097 1080 1067 782 226 152 115 AseI AT'TA_AT Cuts at: 160 1032 18880 160 Size: 872 17848 1606 Fragments arranged by size: 17848 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 20326 AvaI C'yCGr_G Cuts at: 1870 1943 2197 2225 2455 2762 3127 4511 7165 Size: 73 254 28 230 307 365 1384 2654 Cuts at: 7165 7468 7871 8824 9721 11015 11721 12267 13418 Size: 303 403 953 897 1294 706 546 1151 Cuts at: 13418 13829 15041 15621 17651 1870 Size: 411 1212 580 2030 4545 Fragments arranged by size: 4545 2654 2030 1384 1294 1212 1151 953 897 706 580 546 411 403 365 307 303 254 230 73 28 AvrII C'CTAG_G Cuts at: 5117 13807 5117 Size: 8690 11636 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 8340 8373 15502 15535 8340 Size: 33 7129 33 13131 Fragments arranged by size: 13131 7129 33 33 BamHI G'GATC_C Cuts at: 9150 9243 14697 15835 17125 17656 18023 9150 Size: 93 5454 1138 1290 531 367 11453 Fragments arranged by size: 11453 5454 1290 1138 531 367 93 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3799 4082 4784 6652 7266 Size: 325 217 627 2012 283 702 1868 614 Cuts at: 7266 7365 7864 8114 8579 9079 9323 11089 14826 Size: 99 499 250 465 500 244 1766 3737 Cuts at: 14826 14982 17647 19102 618 Size: 156 2665 1455 1842 Fragments arranged by size: 3737 2665 2012 1868 1842 1766 1455 702 627 614 500 499 465 325 283 250 244 217 156 99 BanII G_rGCy'C Cuts at: 1391 2186 2204 2235 2727 2763 3128 3470 5018 Size: 795 18 31 492 36 365 342 1548 Cuts at: 5018 5648 5784 6204 6716 7152 7296 7661 8336 Size: 630 136 420 512 436 144 365 675 Cuts at: 8336 9189 9677 10370 10669 10734 11577 12028 13689 Size: 853 488 693 299 65 843 451 1661 Cuts at: 13689 13759 15396 17336 17645 1391 Size: 70 1637 1940 309 4072 Fragments arranged by size: 4072 1940 1661 1637 1548 853 843 795 693 675 630 512 492 488 451 436 420 365 365 342 309 299 144 136 70 65 36 31 18 BbeI G_GCGC'C Cuts at: 4086 8118 4086 Size: 4032 16294 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2885 3021 3196 4702 5176 6569 6832 Size: 971 986 136 175 1506 474 1393 263 Cuts at: 6832 7776 7840 10252 10560 10583 10605 10620 11647 Size: 944 64 2412 308 23 22 15 1027 Cuts at: 11647 12456 13852 13871 13907 14564 15465 15730 928 Size: 809 1396 19 36 657 901 265 5524 Fragments arranged by size: 5524 2412 1506 1396 1393 1027 986 971 944 901 809 657 474 308 265 263 175 136 64 36 23 22 19 15 BbvCI CC'TCA_GC Cuts at: 2803 5561 7337 9543 12181 13970 16337 16442 17354 Size: 2758 1776 2206 2638 1789 2367 105 912 Cuts at: 17354 2803 Size: 5775 Fragments arranged by size: 5775 2758 2638 2367 2206 1789 1776 912 105 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2951 2985 4331 4365 7459 7493 14702 Size: 34 1014 34 1346 34 3094 34 7209 Cuts at: 14702 14736 18517 18551 1903 Size: 34 3781 34 3678 Fragments arranged by size: 7209 3781 3678 3094 1346 1014 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3999 5051 12757 14816 18218 19428 3999 Size: 1052 7706 2059 3402 1210 4897 Fragments arranged by size: 7706 4897 3402 2059 1210 1052 BclI T'GATC_A Cuts at: 1548 5100 9514 9846 11055 17176 1548 Size: 3552 4414 332 1209 6121 4698 Fragments arranged by size: 6121 4698 4414 3552 1209 332 BfrBI ATG'CAT Cuts at: 4053 4998 10608 4053 Size: 945 5610 13771 Fragments arranged by size: 13771 5610 945 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2436 2813 7538 Size: 107 122 71 631 605 764 377 4725 Cuts at: 7538 7892 13595 14663 15428 16626 18937 136 Size: 354 5703 1068 765 1198 2311 1525 Fragments arranged by size: 5703 4725 2311 1525 1198 1068 765 764 631 605 377 354 122 107 71 BglII A'GATC_T Cuts at: 8866 10384 11277 12991 20321 8866 Size: 1518 893 1714 7330 8871 Fragments arranged by size: 8871 7330 1714 1518 893 BlpI GC'TnA_GC Cuts at: 2832 3122 4256 5017 6860 7703 11813 14852 16916 Size: 290 1134 761 1843 843 4110 3039 2064 Cuts at: 16916 17346 17743 2832 Size: 430 397 5415 Fragments arranged by size: 5415 4110 3039 2064 1843 1134 843 761 430 397 290 Bme1580I G_kGCm'C Cuts at: 1790 2186 2462 5981 7671 7754 8440 9084 9360 Size: 396 276 3519 1690 83 686 644 276 Cuts at: 9360 10189 10370 10669 12860 13449 14052 16302 18387 Size: 829 181 299 2191 589 603 2250 2085 Cuts at: 18387 19543 1790 Size: 1156 2573 Fragments arranged by size: 3519 2573 2250 2191 2085 1690 1156 829 686 644 603 589 396 299 276 276 181 83 BmgBI CAC'GTC Cuts at: 1318 5182 6912 8176 9925 13858 13897 15703 1318 Size: 3864 1730 1264 1749 3933 39 1806 5941 Fragments arranged by size: 5941 3933 3864 1806 1749 1730 1264 39 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3661 3842 7813 Size: 416 53 83 399 24 2244 181 3971 Cuts at: 7813 8356 9024 10672 13629 13753 15065 15725 16042 Size: 543 668 1648 2957 124 1312 660 317 Cuts at: 16042 16163 18057 19015 442 Size: 121 1894 958 1753 Fragments arranged by size: 3971 2957 2244 1894 1753 1648 1312 958 668 660 543 416 399 317 181 124 121 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 20326 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2097 4195 16457 17502 17504 2097 Size: 2098 12262 1045 2 4919 Fragments arranged by size: 12262 4919 2098 1045 2 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2498 3509 4159 4190 4840 5342 6209 8582 Size: 1259 1011 650 31 650 502 867 2373 Cuts at: 8582 9056 9373 11531 11564 11951 11953 12827 12830 Size: 474 317 2158 33 387 2 874 3 Cuts at: 12830 13181 13703 14121 14557 14558 14616 15515 15810 Size: 351 522 418 436 1 58 899 295 Cuts at: 15810 15896 16091 16244 16883 18986 1239 Size: 86 195 153 639 2103 2579 Fragments arranged by size: 2579 2373 2158 2103 1259 1011 899 874 867 650 650 639 522 502 474 436 418 387 351 317 295 195 153 86 58 33 31 3 2 1 Bpu10I CC'TnA_GC Cuts at: 2803 3469 3989 5561 5642 7139 7151 7337 9302 Size: 666 520 1572 81 1497 12 186 1965 Cuts at: 9302 9543 12181 12423 13970 16337 16442 17354 2803 Size: 241 2638 242 1547 2367 105 912 5775 Fragments arranged by size: 5775 2638 2367 1965 1572 1547 1497 912 666 520 242 241 186 105 81 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3005 4430 5831 9146 14573 15456 16553 16907 17139 Size: 1425 1401 3315 5427 883 1097 354 232 Cuts at: 17139 18451 19316 19614 3005 Size: 1312 865 298 3717 Fragments arranged by size: 5427 3717 3315 1425 1401 1312 1097 883 865 354 298 232 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2939 7397 7452 12642 15908 16835 18989 Size: 617 1440 4458 55 5190 3266 927 2154 Cuts at: 18989 882 Size: 2219 Fragments arranged by size: 5190 4458 3266 2219 2154 1440 927 617 55 BsaAI yAC'GTr Cuts at: 493 1204 3796 8008 8354 10709 11152 11389 13801 Size: 711 2592 4212 346 2355 443 237 2412 Cuts at: 13801 14047 17191 18117 493 Size: 246 3144 926 2702 Fragments arranged by size: 4212 3144 2702 2592 2412 2355 926 711 443 346 246 237 BsaBI GATnn'nnATC Cuts at: 15812 20320 15812 Size: 4508 15818 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2129 4083 8115 8315 Size: 53 83 186 936 596 1954 4032 200 Cuts at: 8315 9036 10356 11469 11652 11676 13406 17385 18133 Size: 721 1320 1113 183 24 1730 3979 748 Cuts at: 18133 18515 19150 275 Size: 382 635 1451 Fragments arranged by size: 4032 3979 1954 1730 1451 1320 1113 936 748 721 635 596 382 200 186 183 83 53 24 BsaWI w'CCGG_w Cuts at: 2666 4278 8886 9234 9585 12339 18759 19157 19431 Size: 1612 4608 348 351 2754 6420 398 274 Cuts at: 19431 19578 19989 2666 Size: 147 411 3003 Fragments arranged by size: 6420 4608 3003 2754 1612 411 398 351 348 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2102 2132 2477 2507 2714 Size: 30 165 30 699 30 345 30 207 Cuts at: 2714 2744 2899 2929 3129 3159 4508 4538 5429 Size: 30 155 30 200 30 1349 30 891 Cuts at: 5429 5459 5630 5660 6332 6362 8165 8195 8787 Size: 30 171 30 672 30 1803 30 592 Cuts at: 8787 8817 11837 11867 12024 12054 13154 13184 14759 Size: 30 3020 30 157 30 1100 30 1575 Cuts at: 14759 14789 1178 Size: 30 6715 Fragments arranged by size: 6715 3020 1803 1575 1349 1100 891 699 672 592 345 207 200 171 165 157 155 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2243 2904 3150 3228 3231 3868 4529 5599 Size: 1057 661 246 78 3 637 661 1070 Cuts at: 5599 6743 6767 7291 7634 7745 8173 8213 8344 Size: 1144 24 524 343 111 428 40 131 Cuts at: 8344 8458 9614 9668 10750 11585 11774 12476 12530 Size: 114 1156 54 1082 835 189 702 54 Cuts at: 12530 12683 13178 13233 13361 14279 16537 16844 17342 Size: 153 495 55 128 918 2258 307 498 Cuts at: 17342 17465 1186 Size: 123 4047 Fragments arranged by size: 4047 2258 1156 1144 1082 1070 1057 918 835 702 661 661 637 524 498 495 428 343 307 246 189 153 131 128 123 114 111 78 55 54 54 40 24 3 BseYI C'CCAG_C Cuts at: 2418 4078 4876 4947 5666 6291 6446 6890 9670 Size: 1660 798 71 719 625 155 444 2780 Cuts at: 9670 11247 11827 12168 12427 13263 15418 15679 15895 Size: 1577 580 341 259 836 2155 261 216 Cuts at: 15895 17015 19529 20025 2418 Size: 1120 2514 496 2719 Fragments arranged by size: 2780 2719 2514 2155 1660 1577 1120 836 798 719 625 580 496 444 341 261 259 216 155 71 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3069 3248 4082 6035 6431 6677 6866 Size: 573 1333 179 834 1953 396 246 189 Cuts at: 6866 6872 7195 7582 8210 9922 10450 10568 11900 Size: 6 323 387 628 1712 528 118 1332 Cuts at: 11900 12644 12649 13109 13369 13585 14900 16868 17267 Size: 744 5 460 260 216 1315 1968 399 Cuts at: 17267 17387 1163 Size: 120 4102 Fragments arranged by size: 4102 1968 1953 1712 1333 1332 1315 834 744 628 573 528 460 399 396 387 323 260 246 216 189 179 120 118 6 5 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 9663 14902 17776 18537 19565 1540 Size: 275 186 7662 5239 2874 761 1028 2301 Fragments arranged by size: 7662 5239 2874 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2727 4344 5018 5648 5981 6204 7050 7098 7145 Size: 1617 674 630 333 223 846 48 47 Cuts at: 7145 7152 7296 7754 7812 8163 8336 9189 9360 Size: 7 144 458 58 351 173 853 171 Cuts at: 9360 9966 10734 11030 11577 12693 13449 13689 13707 Size: 606 768 296 547 1116 756 240 18 Cuts at: 13707 13800 13806 13857 13997 14052 17114 18387 18472 Size: 93 6 51 140 55 3062 1273 85 Cuts at: 18472 19543 2727 Size: 1071 3510 Fragments arranged by size: 3510 3062 1617 1273 1116 1071 853 846 768 756 674 630 606 547 458 351 333 296 240 223 173 171 144 140 93 85 58 55 51 48 47 18 7 6 BsmI GAATG_Cn' Cuts at: 6464 8597 9997 13949 14486 15227 17837 17930 6464 Size: 2133 1400 3952 537 741 2610 93 8860 Fragments arranged by size: 8860 3952 2610 2133 1400 741 537 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2957 9027 9650 10522 12933 15693 16529 2957 Size: 6070 623 872 2411 2760 836 6754 Fragments arranged by size: 6754 6070 2760 2411 872 836 623 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2186 2204 2235 2462 2727 2763 3128 Size: 399 396 18 31 227 265 36 365 Cuts at: 3128 3470 4344 5018 5648 5784 5981 6204 6716 Size: 342 874 674 630 136 197 223 512 Cuts at: 6716 7050 7098 7145 7152 7296 7661 7671 7754 Size: 334 48 47 7 144 365 10 83 Cuts at: 7754 7812 8163 8336 8440 9084 9189 9360 9677 Size: 58 351 173 104 644 105 171 317 Cuts at: 9677 9966 10189 10370 10669 10734 11030 11577 12028 Size: 289 223 181 299 65 296 547 451 Cuts at: 12028 12693 12860 13449 13689 13707 13759 13800 13806 Size: 665 167 589 240 18 52 41 6 Cuts at: 13806 13857 13997 14052 15396 16302 17114 17336 17645 Size: 51 140 55 1344 906 812 222 309 Cuts at: 17645 18387 18472 19543 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1344 1071 906 874 812 742 674 665 644 630 589 547 512 451 399 396 365 365 351 342 334 317 309 299 296 289 265 240 227 223 223 222 197 181 173 171 167 144 140 136 105 104 85 83 65 58 55 52 51 48 47 41 36 31 18 18 10 7 6 BspEI T'CCGG_A Cuts at: 8886 12339 19989 8886 Size: 3453 7650 9223 Fragments arranged by size: 9223 7650 3453 BspHI T'CATG_A Cuts at: 3286 3534 6468 8766 9039 9879 10263 14434 18215 Size: 248 2934 2298 273 840 384 4171 3781 Cuts at: 18215 19945 3286 Size: 1730 3667 Fragments arranged by size: 4171 3781 3667 2934 2298 1730 840 384 273 248 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 6057 6076 6525 6888 7471 7886 7976 8405 Size: 5213 19 449 363 583 415 90 429 Cuts at: 8405 9318 10396 10512 11325 11689 11922 13242 13965 Size: 913 1078 116 813 364 233 1320 723 Cuts at: 13965 14688 14790 16197 17681 844 Size: 723 102 1407 1484 3489 Fragments arranged by size: 5213 3489 1484 1407 1320 1078 913 813 723 723 583 449 429 415 364 363 233 116 102 90 19 BsrBI CCG'CTC Cuts at: 1394 1823 4166 14397 16649 16783 18213 1394 Size: 429 2343 10231 2252 134 1430 3507 Fragments arranged by size: 10231 3507 2343 2252 1430 429 134 BsrDI GCAATG_nn' Cuts at: 65 1248 3803 10938 12174 13599 15197 16210 17229 Size: 1183 2555 7135 1236 1425 1598 1013 1019 Cuts at: 17229 18821 18995 65 Size: 1592 174 1396 Fragments arranged by size: 7135 2555 1598 1592 1425 1396 1236 1183 1019 1013 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2666 4395 7950 11505 15668 18970 19157 Size: 110 856 1729 3555 3555 4163 3302 187 Cuts at: 19157 1700 Size: 2869 Fragments arranged by size: 4163 3555 3555 3302 2869 1729 856 187 110 BsrGI T'GTAC_A Cuts at: 96 11475 14514 15150 16944 96 Size: 11379 3039 636 1794 3478 Fragments arranged by size: 11379 3478 3039 1794 636 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 20326 BssSI C'ACGA_G Cuts at: 6725 6735 9959 11132 15269 18386 19398 6725 Size: 10 3224 1173 4137 3117 1012 7653 Fragments arranged by size: 7653 4137 3224 3117 1173 1012 10 BstAPI GCAn_nnn'nTGC Cuts at: 794 2064 2822 3315 4252 4944 10591 11167 14056 Size: 1270 758 493 937 692 5647 576 2889 Cuts at: 14056 14950 15585 16318 794 Size: 894 635 733 4802 Fragments arranged by size: 5647 4802 2889 1270 937 894 758 733 692 635 576 493 BstBI TT'CG_AA Cuts at: 19130 19130 Size: 20326 BstEII G'GTnAC_C Cuts at: 1184 5285 5896 8758 8839 13846 16163 1184 Size: 4101 611 2862 81 5007 2317 5347 Fragments arranged by size: 5347 5007 4101 2862 2317 611 81 BstXI CCAn_nnnn'nTGG Cuts at: 2426 2476 3280 4001 6550 9346 10240 12355 13271 Size: 50 804 721 2549 2796 894 2115 916 Cuts at: 13271 13681 14005 15895 15925 2426 Size: 410 324 1890 30 6827 Fragments arranged by size: 6827 2796 2549 2115 1890 916 894 804 721 410 324 50 30 BstYI r'GATC_y Cuts at: 1972 5878 8467 8866 9150 9243 10039 10192 10384 Size: 3906 2589 399 284 93 796 153 192 Cuts at: 10384 11163 11277 12344 12991 14697 15155 15228 15835 Size: 779 114 1067 647 1706 458 73 607 Cuts at: 15835 16381 16585 16605 17125 17326 17656 18023 18408 Size: 546 204 20 520 201 330 367 385 Cuts at: 18408 18425 19877 19963 19975 20321 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 3906 2589 1977 1706 1452 1067 796 779 647 607 546 520 458 399 385 367 346 330 284 204 201 192 153 114 93 86 73 20 17 12 BstZ17I GTA'TAC Cuts at: 9700 17151 9700 Size: 7451 12875 Bsu36I CC'TnA_GG Cuts at: 2388 4149 5745 7217 8088 8441 9145 9917 10955 Size: 1761 1596 1472 871 353 704 772 1038 Cuts at: 10955 13512 14066 14742 2388 Size: 2557 554 676 7972 Fragments arranged by size: 7972 2557 1761 1596 1472 1038 871 772 704 676 554 353 BtgI C'CryG_G Cuts at: 513 1065 2004 2381 4121 4848 5124 5982 6615 Size: 552 939 377 1740 727 276 858 633 Cuts at: 6615 6921 7452 8844 11802 12301 12522 12633 13270 Size: 306 531 1392 2958 499 221 111 637 Cuts at: 13270 13439 15426 15540 16695 16731 513 Size: 169 1987 114 1155 36 4108 Fragments arranged by size: 4108 2958 1987 1740 1392 1155 939 858 727 637 633 552 531 499 377 306 276 221 169 114 111 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 9986 10108 10213 12482 15012 15249 Size: 683 56 8760 122 105 2269 2530 237 Cuts at: 15249 17076 487 Size: 1827 3737 Fragments arranged by size: 8760 3737 2530 2269 1827 683 237 122 105 56 BtsI GCAGTG_nn' Cuts at: 2929 4099 4926 6853 7810 8614 8995 10481 11156 Size: 1170 827 1927 957 804 381 1486 675 Cuts at: 11156 12583 14050 14780 17842 18636 18656 2929 Size: 1427 1467 730 3062 794 20 4599 Fragments arranged by size: 4599 3062 1927 1486 1467 1427 1170 957 827 804 794 730 675 381 20 ClaI AT'CG_AT Cuts at: 1554 11771 12330 1554 Size: 10217 559 9550 Fragments arranged by size: 10217 9550 559 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2491 2526 529 Size: 35 1927 35 18329 Fragments arranged by size: 18329 1927 35 35 DraI TTT'AAA Cuts at: 5148 12774 15990 17630 5148 Size: 7626 3216 1640 7844 Fragments arranged by size: 7844 7626 3216 1640 DraIII CAC_nnn'GTG Cuts at: 10417 14833 16270 10417 Size: 4416 1437 14473 Fragments arranged by size: 14473 4416 1437 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 10772 19333 809 Size: 507 9456 8561 1802 Fragments arranged by size: 9456 8561 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2437 2556 4977 Size: 54 1425 50 275 18 607 119 2421 Cuts at: 4977 6612 6618 6664 7411 7486 7510 9529 9660 Size: 1635 6 46 747 75 24 2019 131 Cuts at: 9660 10065 11004 11377 12454 14760 14899 15027 15342 Size: 405 939 373 1077 2306 139 128 315 Cuts at: 15342 15526 15670 15885 16146 17773 18662 20291 8 Size: 184 144 215 261 1627 889 1629 43 Fragments arranged by size: 2421 2306 2019 1635 1629 1627 1425 1077 939 889 747 607 405 373 315 275 261 215 184 144 139 131 128 119 75 54 50 46 43 24 18 6 EagI C'GGCC_G Cuts at: 1537 1812 9660 14899 17773 1537 Size: 275 7848 5239 2874 4090 Fragments arranged by size: 7848 5239 4090 2874 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3174 3758 4280 5714 7061 7284 8006 8341 Size: 1821 584 522 1434 1347 223 722 335 Cuts at: 8341 8708 8885 10304 10394 11454 11552 11621 12271 Size: 367 177 1419 90 1060 98 69 650 Cuts at: 12271 12780 13419 13793 14297 14528 14595 17547 18256 Size: 509 639 374 504 231 67 2952 709 Cuts at: 18256 1353 Size: 3423 Fragments arranged by size: 3423 2952 1821 1434 1419 1347 1060 722 709 650 639 584 522 509 504 374 367 335 231 223 177 98 90 69 67 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6529 10565 11432 12461 12966 14241 Size: 1064 138 4940 4036 867 1029 505 1275 Cuts at: 14241 14690 17450 18913 19287 19433 387 Size: 449 2760 1463 374 146 1280 Fragments arranged by size: 4940 4036 2760 1463 1280 1275 1064 1029 867 505 449 374 146 138 EcoICRI GAG'CTC Cuts at: 2725 5016 5646 6202 7150 7294 8334 9187 10732 Size: 2291 630 556 948 144 1040 853 1545 Cuts at: 10732 11575 13687 2725 Size: 843 2112 9364 Fragments arranged by size: 9364 2291 2112 1545 1040 948 853 843 630 556 144 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2189 2498 2727 2997 3033 3509 3601 Size: 393 557 309 229 270 36 476 92 Cuts at: 3601 4159 4190 4211 4764 4840 5030 5170 5342 Size: 558 31 21 553 76 190 140 172 Cuts at: 5342 5507 6103 6209 6347 6442 6581 7816 8322 Size: 165 596 106 138 95 139 1235 506 Cuts at: 8322 8582 9056 9373 10307 10421 10595 10948 11531 Size: 260 474 317 934 114 174 353 583 Cuts at: 11531 11564 11888 11951 11953 12827 12830 13181 13280 Size: 33 324 63 2 874 3 351 99 Cuts at: 13280 13649 13703 14121 14517 14557 14558 14616 15095 Size: 369 54 418 396 40 1 58 479 Cuts at: 15095 15477 15515 15810 15887 15896 16091 16244 16883 Size: 382 38 295 77 9 195 153 639 Cuts at: 16883 18389 18986 19773 20041 1239 Size: 1506 597 787 268 1524 Fragments arranged by size: 1524 1506 1235 934 874 787 639 597 596 583 558 557 553 506 479 476 474 418 396 393 382 369 353 351 324 317 309 295 270 268 260 229 195 190 174 172 165 153 140 139 138 114 106 99 95 92 77 76 63 58 54 40 38 36 33 31 21 9 3 2 1 EcoNI CCTnn'n_nnAGG Cuts at: 2654 3572 5116 6053 6275 9056 10403 10775 13387 Size: 918 1544 937 222 2781 1347 372 2612 Cuts at: 13387 13730 14600 14684 14745 16322 16375 2654 Size: 343 870 84 61 1577 53 6605 Fragments arranged by size: 6605 2781 2612 1577 1544 1347 937 918 870 372 343 222 84 61 53 EcoO109I rG'GnC_Cy Cuts at: 2182 2799 3565 4719 7233 7909 8152 9049 9061 Size: 617 766 1154 2514 676 243 897 12 Cuts at: 9061 10366 10501 10666 11406 11717 14020 14070 14884 Size: 1305 135 165 740 311 2303 50 814 Cuts at: 14884 15302 15871 16627 16786 2182 Size: 418 569 756 159 5722 Fragments arranged by size: 5722 2514 2303 1305 1154 897 814 766 756 740 676 617 569 418 311 243 165 159 135 50 12 EcoRI G'AATT_C Cuts at: 10850 14129 17635 18013 10850 Size: 3279 3506 378 13163 Fragments arranged by size: 13163 3506 3279 378 EcoRV GAT'ATC Cuts at: 11530 12924 15247 11530 Size: 1394 2323 16609 Fragments arranged by size: 16609 2323 1394 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4739 5200 5695 6097 10660 10870 14326 14446 4739 Size: 461 495 402 4563 210 3456 120 10619 Fragments arranged by size: 10619 4563 3456 495 461 402 210 120 FspI TGC'GCA Cuts at: 8139 13931 15944 18832 8139 Size: 5792 2013 2888 9633 Fragments arranged by size: 9633 5792 2888 2013 FspAI rTGC'GCAy Cuts at: 13931 13931 Size: 20326 HaeII r_GCGC'y Cuts at: 1678 4086 8118 8345 8876 9934 10746 10840 11820 Size: 2408 4032 227 531 1058 812 94 980 Cuts at: 11820 11938 12168 12633 13418 14311 17458 19473 1678 Size: 118 230 465 785 893 3147 2015 2531 Fragments arranged by size: 4032 3147 2531 2408 2015 1058 980 893 812 785 531 465 230 227 118 94 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2477 2509 3947 3979 4508 Size: 32 135 32 905 32 1438 32 529 Cuts at: 4508 4531 4540 4563 5870 5902 5994 6026 6332 Size: 23 9 23 1307 32 92 32 306 Cuts at: 6332 6364 6869 6901 8071 8103 8165 8197 10029 Size: 32 505 32 1170 32 62 32 1832 Cuts at: 10029 10061 11102 11134 11701 11733 11753 11785 11837 Size: 32 1041 32 567 32 20 32 52 Cuts at: 11837 11869 12336 12368 13918 13950 14117 14149 14207 Size: 32 467 32 1550 32 167 32 58 Cuts at: 14207 14239 14717 14749 16287 16319 18967 18999 19041 Size: 32 478 32 1538 32 2648 32 42 Cuts at: 19041 19073 1373 Size: 32 2626 Fragments arranged by size: 2648 2626 1832 1550 1538 1438 1307 1170 1041 905 567 529 505 478 467 306 167 135 92 62 58 52 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 23 23 20 9 HincII GTy'rAC Cuts at: 669 1657 1938 3433 5271 9094 13341 15123 16011 Size: 988 281 1495 1838 3823 4247 1782 888 Cuts at: 16011 17670 17916 669 Size: 1659 246 3079 Fragments arranged by size: 4247 3823 3079 1838 1782 1659 1495 988 888 281 246 HindIII A'AGCT_T Cuts at: 748 10986 748 Size: 10238 10088 HpaI GTT'AAC Cuts at: 15123 17916 15123 Size: 2793 17533 KasI G'GCGC_C Cuts at: 4082 8114 4082 Size: 4032 16294 KpnI G_GTAC'C Cuts at: 3803 7270 9327 14986 17651 3803 Size: 3467 2057 5659 2665 6478 Fragments arranged by size: 6478 5659 3467 2665 2057 MfeI C'AATT_G Cuts at: 17925 17925 Size: 20326 MluI A'CGCG_T Cuts at: 12720 20202 12720 Size: 7482 12844 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5293 7972 8883 9423 10683 13146 13523 13748 Size: 3988 2679 911 540 1260 2463 377 225 Cuts at: 13748 15470 16833 19440 19624 1305 Size: 1722 1363 2607 184 2007 Fragments arranged by size: 3988 2679 2607 2463 2007 1722 1363 1260 911 540 377 225 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2439 2558 4979 6614 6666 Size: 54 1425 343 607 119 2421 1635 52 Cuts at: 6666 7413 7512 9531 10067 11006 14762 15029 15344 Size: 747 99 2019 536 939 3756 267 315 Cuts at: 15344 15887 16148 10 Size: 543 261 4188 Fragments arranged by size: 4188 3756 2421 2019 1635 1425 939 747 607 543 536 343 315 267 261 119 99 54 52 MslI CAynn'nnrTG Cuts at: 518 1166 3727 4131 5591 6377 6641 6818 8945 Size: 648 2561 404 1460 786 264 177 2127 Cuts at: 8945 9224 10073 10700 10886 11991 12043 12330 12353 Size: 279 849 627 186 1105 52 287 23 Cuts at: 12353 13065 13085 13334 13607 15923 16940 18284 18643 Size: 712 20 249 273 2316 1017 1344 359 Cuts at: 18643 18802 518 Size: 159 2042 Fragments arranged by size: 2561 2316 2127 2042 1460 1344 1105 1017 849 786 712 648 627 404 359 287 279 273 264 249 186 177 159 52 23 20 MspA1I CmG'CkG Cuts at: 1606 3053 3438 3593 3727 4123 4850 5126 5734 Size: 1447 385 155 134 396 727 276 608 Cuts at: 5734 6359 7062 7129 7300 7644 7702 9626 9694 Size: 625 703 67 171 344 58 1924 68 Cuts at: 9694 9988 10591 11053 11843 12400 13140 13681 13719 Size: 294 603 462 790 557 740 541 38 Cuts at: 13719 14141 14863 15694 16052 16474 16876 17047 17117 Size: 422 722 831 358 422 402 171 70 Cuts at: 17117 17140 17272 17571 17594 17742 18419 19567 19812 Size: 23 132 299 23 148 677 1148 245 Cuts at: 19812 1606 Size: 2120 Fragments arranged by size: 2120 1924 1447 1148 831 790 740 727 722 703 677 625 608 603 557 541 462 422 422 402 396 385 358 344 299 294 276 245 171 171 155 148 134 132 70 68 67 58 38 23 23 NarI GG'CG_CC Cuts at: 4083 8115 4083 Size: 4032 16294 NcoI C'CATG_G Cuts at: 513 1065 2004 2381 6921 8844 12522 13270 15426 Size: 552 939 377 4540 1923 3678 748 2156 Cuts at: 15426 513 Size: 5413 Fragments arranged by size: 5413 4540 3678 2156 1923 939 748 552 377 NdeI CA'TA_TG Cuts at: 387 9519 387 Size: 9132 11194 NheI G'CTAG_C Cuts at: 1052 1052 Size: 20326 NotI GC'GGCC_GC Cuts at: 17773 17773 Size: 20326 NsiI A_TGCA'T Cuts at: 4055 5000 10610 4055 Size: 945 5610 13771 Fragments arranged by size: 13771 5610 945 NspI r_CATG'y Cuts at: 1132 1229 2030 4771 7552 12386 12643 13711 14180 Size: 97 801 2741 2781 4834 257 1068 469 Cuts at: 14180 14521 15437 15714 16763 17137 17503 17684 19229 Size: 341 916 277 1049 374 366 181 1545 Cuts at: 19229 1132 Size: 2229 Fragments arranged by size: 4834 2781 2741 2229 1545 1068 1049 916 801 469 374 366 341 277 257 181 97 PasI CC'CwG_GG Cuts at: 1348 7654 7663 12981 1348 Size: 6306 9 5318 8693 Fragments arranged by size: 8693 6306 5318 9 PciI A'CATG_T Cuts at: 15710 19225 15710 Size: 3515 16811 PflMI CCAn_nnn'nTGG Cuts at: 1108 2539 5662 7420 9538 9805 10990 13555 15679 Size: 1431 3123 1758 2118 267 1185 2565 2124 Cuts at: 15679 16308 1108 Size: 629 5126 Fragments arranged by size: 5126 3123 2565 2124 2118 1758 1431 1185 629 267 PfoI T'CCnGG_A Cuts at: 2249 2592 3099 4194 4368 4402 4485 6225 6297 Size: 343 507 1095 174 34 83 1740 72 Cuts at: 6297 8130 8301 10776 12591 12846 2249 Size: 1833 171 2475 1815 255 9729 Fragments arranged by size: 9729 2475 1833 1815 1740 1095 507 343 255 174 171 83 72 34 PmeI GTTT'AAAC Cuts at: 17630 17630 Size: 20326 PmlI CAC'GTG Cuts at: 3796 8354 10709 13801 14047 17191 3796 Size: 4558 2355 3092 246 3144 6931 Fragments arranged by size: 6931 4558 3144 3092 2355 246 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2102 2134 3377 3409 9215 9247 18395 18427 19908 Size: 32 1243 32 5806 32 9148 32 1481 Cuts at: 19908 19940 2102 Size: 32 2488 Fragments arranged by size: 9148 5806 2488 1481 1243 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3565 4719 7909 8152 9049 10501 11406 11717 14020 Size: 1154 3190 243 897 1452 905 311 2303 Cuts at: 14020 15871 16786 3565 Size: 1851 915 7105 Fragments arranged by size: 7105 3190 2303 1851 1452 1154 915 905 897 311 243 PshAI GACnn'nnGTC Cuts at: 1613 3282 11397 13875 14387 1613 Size: 1669 8115 2478 512 7552 Fragments arranged by size: 8115 7552 2478 1669 512 PsiI TTA'TAA Cuts at: 3369 5333 12200 17896 3369 Size: 1964 6867 5696 5799 Fragments arranged by size: 6867 5799 5696 1964 PspOMI G'GGCC_C Cuts at: 2182 10366 10665 2182 Size: 8184 299 11843 Fragments arranged by size: 11843 8184 299 PspXI vC'TCGA_Gb Cuts at: 7871 15041 7871 Size: 7170 13156 PstI C_TGCA'G Cuts at: 830 2060 2089 2529 2778 3630 4574 4634 4669 Size: 1230 29 440 249 852 944 60 35 Cuts at: 4669 6261 6848 7037 7482 8402 8486 8973 9376 Size: 1592 587 189 445 920 84 487 403 Cuts at: 9376 10509 10903 11163 11172 11258 11322 11700 12054 Size: 1133 394 260 9 86 64 378 354 Cuts at: 12054 12381 13368 13482 14725 14787 15358 15855 16235 Size: 327 987 114 1243 62 571 497 380 Cuts at: 16235 16323 16574 17052 17678 830 Size: 88 251 478 626 3478 Fragments arranged by size: 3478 1592 1243 1230 1133 987 944 920 852 626 587 571 497 487 478 445 440 403 394 380 378 354 327 260 251 249 189 114 88 86 84 64 62 60 35 29 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 20326 PvuII CAG'CTG Cuts at: 3053 3727 7062 7129 7702 9626 9694 9988 11053 Size: 674 3335 67 573 1924 68 294 1065 Cuts at: 11053 12400 13140 14141 16052 16474 17047 17117 17140 Size: 1347 740 1001 1911 422 573 70 23 Cuts at: 17140 17272 17571 17594 3053 Size: 132 299 23 5785 Fragments arranged by size: 5785 3335 1924 1911 1347 1065 1001 740 674 573 573 422 299 294 132 70 68 67 23 23 SacI G_AGCT'C Cuts at: 2727 5018 5648 6204 7152 7296 8336 9189 10734 Size: 2291 630 556 948 144 1040 853 1545 Cuts at: 10734 11577 13689 2727 Size: 843 2112 9364 Fragments arranged by size: 9364 2291 2112 1545 1040 948 853 843 630 556 144 SacII CC_GC'GG Cuts at: 4124 4851 5127 4124 Size: 727 276 19323 Fragments arranged by size: 19323 727 276 SalI G'TCGA_C Cuts at: 1936 9092 17668 1936 Size: 7156 8576 4594 Fragments arranged by size: 8576 7156 4594 SanDI GG'GwC_CC Cuts at: 4719 7909 11406 11717 4719 Size: 3190 3497 311 13328 Fragments arranged by size: 13328 3497 3190 311 SapI GCTCTTCn'nnn_ Cuts at: 5714 7284 8341 11552 14528 5714 Size: 1570 1057 3211 2976 11512 Fragments arranged by size: 11512 3211 2976 1570 1057 SbfI CC_TGCA'GG Cuts at: 2529 8402 17678 2529 Size: 5873 9276 5177 Fragments arranged by size: 9276 5873 5177 ScaI AGT'ACT Cuts at: 3488 6743 11360 11864 12071 18574 20286 3488 Size: 3255 4617 504 207 6503 1712 3528 Fragments arranged by size: 6503 4617 3528 3255 1712 504 207 SexAI A'CCwGG_T Cuts at: 3567 4029 4311 6978 10413 14217 14838 14985 16821 Size: 462 282 2667 3435 3804 621 147 1836 Cuts at: 16821 3567 Size: 7072 Fragments arranged by size: 7072 3804 3435 2667 1836 621 462 282 147 SfcI C'TryA_G Cuts at: 826 1045 2056 2085 2525 2774 2826 3626 4570 Size: 219 1011 29 440 249 52 800 944 Cuts at: 4570 4630 4665 6257 6844 7033 7478 7678 8398 Size: 60 35 1592 587 189 445 200 720 Cuts at: 8398 8482 8969 9372 9637 10505 10873 10899 11159 Size: 84 487 403 265 868 368 26 260 Cuts at: 11159 11168 11254 11318 11696 12050 12377 13364 13478 Size: 9 86 64 378 354 327 987 114 Cuts at: 13478 13652 14721 14783 15354 15851 16231 16319 16570 Size: 174 1069 62 571 497 380 88 251 Cuts at: 16570 17048 17056 17674 18809 19490 19681 826 Size: 478 8 618 1135 681 191 1471 Fragments arranged by size: 1592 1471 1135 1069 1011 987 944 868 800 720 681 618 587 571 497 487 478 445 440 403 380 378 368 354 327 265 260 251 249 219 200 191 189 174 114 88 86 84 64 62 60 52 35 29 26 9 8 SfiI GGCCn_nnn'nGGCC Cuts at: 2436 2436 Size: 20326 SfoI GGC'GCC Cuts at: 4084 8116 4084 Size: 4032 16294 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 20326 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 20326 SmaI CCC'GGG Cuts at: 1872 2199 2457 2764 3129 8826 11723 12269 17653 Size: 327 258 307 365 5697 2897 546 5384 Cuts at: 17653 1872 Size: 4545 Fragments arranged by size: 5697 5384 4545 2897 546 365 327 307 258 SmlI C'TyrA_G Cuts at: 820 1017 1943 3020 4409 4589 5810 5966 7228 Size: 197 926 1077 1389 180 1221 156 1262 Cuts at: 7228 7871 9125 14311 14552 14618 15041 15471 16532 Size: 643 1254 5186 241 66 423 430 1061 Cuts at: 16532 16922 17154 18430 19331 19593 820 Size: 390 232 1276 901 262 1553 Fragments arranged by size: 5186 1553 1389 1276 1262 1254 1221 1077 1061 926 901 643 430 423 390 262 241 232 197 180 156 66 SnaBI TAC'GTA Cuts at: 493 18117 493 Size: 17624 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 20326 SphI G_CATG'C Cuts at: 1132 12643 14180 15437 17684 1132 Size: 11511 1537 1257 2247 3774 Fragments arranged by size: 11511 3774 2247 1537 1257 SspI AAT'ATT Cuts at: 5 52 14462 18250 5 Size: 47 14410 3788 2081 Fragments arranged by size: 14410 3788 2081 47 StuI AGG'CCT Cuts at: 1517 2649 3873 4520 6869 8404 8696 14666 15080 Size: 1132 1224 647 2349 1535 292 5970 414 Cuts at: 15080 17201 1517 Size: 2121 4642 Fragments arranged by size: 5970 4642 2349 2121 1535 1224 1132 647 414 292 StyI C'CwwG_G Cuts at: 513 1065 2004 2034 2381 2955 4000 4714 5117 Size: 552 939 30 347 574 1045 714 403 Cuts at: 5117 6072 6921 7408 8844 10746 10789 11229 12522 Size: 955 849 487 1436 1902 43 440 1293 Cuts at: 12522 12889 13270 13807 14763 14925 15426 16710 17765 Size: 367 381 537 956 162 501 1284 1055 Cuts at: 17765 513 Size: 3074 Fragments arranged by size: 3074 1902 1436 1293 1284 1055 1045 956 955 939 849 714 574 552 537 501 487 440 403 381 367 347 162 43 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3680 3749 6033 6670 7430 8194 8928 Size: 1255 2100 69 2284 637 760 764 734 Cuts at: 8928 10576 10797 11261 11387 11575 13432 13865 14905 Size: 1648 221 464 126 188 1857 433 1040 Cuts at: 14905 14979 15732 17011 17162 18353 18370 18523 18708 Size: 74 753 1279 151 1191 17 153 185 Cuts at: 18708 19153 325 Size: 445 1498 Fragments arranged by size: 2284 2100 1857 1648 1498 1279 1255 1191 1040 764 760 753 734 637 464 445 433 221 188 185 153 151 126 74 69 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3486 3744 6741 Size: 274 80 33 51 1443 1509 258 2997 Cuts at: 6741 11241 11358 11475 11862 12069 13185 14424 14514 Size: 4500 117 117 387 207 1116 1239 90 Cuts at: 14514 15150 16944 18572 20284 96 Size: 636 1794 1628 1712 138 Fragments arranged by size: 4500 2997 1794 1712 1628 1509 1443 1239 1116 636 387 274 258 207 138 117 117 90 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3129 3161 3920 3952 5630 5662 6517 6549 8190 Size: 32 759 32 1678 32 855 32 1641 Cuts at: 8190 8222 8787 8819 10403 10435 10935 10951 10967 Size: 32 565 32 1584 32 500 16 16 Cuts at: 10967 10983 11148 11180 11604 11636 12707 12739 13154 Size: 16 165 32 424 32 1071 32 415 Cuts at: 13154 13186 14759 14791 20286 20318 3129 Size: 32 1573 32 5495 32 3137 Fragments arranged by size: 5495 3137 1678 1641 1584 1573 1071 855 759 565 500 424 415 165 32 32 32 32 32 32 32 32 32 32 32 32 32 16 16 16 Tth111I GACn'n_nGTC Cuts at: 1316 7924 10784 14024 14933 1316 Size: 6608 2860 3240 909 6709 Fragments arranged by size: 6709 6608 3240 2860 909 XbaI T'CTAG_A Cuts at: 16078 17662 16078 Size: 1584 18742 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2473 5663 6965 10345 11149 13219 13556 15596 2473 Size: 3190 1302 3380 804 2070 337 2040 7203 Fragments arranged by size: 7203 3380 3190 2070 2040 1302 804 337 XhoI C'TCGA_G Cuts at: 1943 7871 15041 1943 Size: 5928 7170 7228 Fragments arranged by size: 7228 7170 5928 XmaI C'CCGG_G Cuts at: 1870 2197 2455 2762 3127 8824 11721 12267 17651 Size: 327 258 307 365 5697 2897 546 5384 Cuts at: 17651 1870 Size: 4545 Fragments arranged by size: 5697 5384 4545 2897 546 365 327 307 258 XmnI GAAnn'nnTTC Cuts at: 3656 4693 9944 10259 10924 13293 13313 14486 16394 Size: 1037 5251 315 665 2369 20 1173 1908 Cuts at: 16394 17523 18455 3656 Size: 1129 932 5527 Fragments arranged by size: 5527 5251 2369 1908 1173 1129 1037 932 665 315 20 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8316 9037 11470 11653 Size: 53 83 186 936 6782 721 2433 183 Cuts at: 11653 18134 276 Size: 6481 2468 Fragments arranged by size: 6782 6481 2468 2433 936 721 186 183 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BsiWI FseI NaeI NgoMIV NruI PacI PsrI RsrII SrfI SwaI