(Linear) (Six-base) MAPSORT of: KIEE3555.seq Check: 3347 from: 1 to: 15621 KIEE3555.orf With 182 enzymes: SgfI * April 17, 2013 10:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 4052 5971 6400 9917 14192 15621 Size: 4052 1919 429 3517 4275 1429 Fragments arranged by size: 4275 4052 3517 1919 1429 429 AatII G_ACGT'C Cuts at: 0 6313 7034 9467 9650 15621 Size: 6313 721 2433 183 5971 Fragments arranged by size: 6313 5971 2433 721 183 AccI GT'mk_AC Cuts at: 0 5540 5672 7088 7694 12209 14684 15145 15621 Size: 5540 132 1416 606 4515 2475 461 476 Fragments arranged by size: 5540 4515 2475 1416 606 476 461 132 Acc65I G'GTAC_C Cuts at: 0 1794 5261 7318 12977 15621 Size: 1794 3467 2057 5659 2644 Fragments arranged by size: 5659 3467 2644 2057 1794 AclI AA'CG_TT Cuts at: 0 2447 15621 Size: 2447 13174 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 184 722 992 1028 1596 2206 2759 3025 Size: 184 538 270 36 568 610 553 266 Cuts at: 3025 3165 3502 4098 4342 4437 4576 5811 6317 Size: 140 337 596 244 95 139 1235 506 Cuts at: 6317 8302 8416 8590 8943 9883 11275 11644 12512 Size: 1985 114 174 353 940 1392 369 868 Cuts at: 12512 13090 13472 13882 15621 Size: 578 382 410 1739 Fragments arranged by size: 1985 1739 1392 1235 940 868 610 596 578 568 553 538 506 410 382 369 353 337 270 266 244 184 174 140 139 114 95 36 AfeI AGC'GCT Cuts at: 0 6338 7927 15451 15621 Size: 6338 1589 7524 170 Fragments arranged by size: 7524 6338 1589 170 AflII C'TTAA_G Cuts at: 0 2584 3961 5223 12306 12613 15621 Size: 2584 1377 1262 7083 307 3008 Fragments arranged by size: 7083 3008 2584 1377 1262 307 AflIII A'CryG_T Cuts at: 0 1788 6346 6348 6542 10715 13705 15185 15621 Size: 1788 4558 2 194 4173 2990 1480 436 Fragments arranged by size: 4558 4173 2990 1788 1480 436 194 2 AgeI A'CCGG_T Cuts at: 0 661 15621 Size: 661 14960 AhdI GACnn_n'nnGTC Cuts at: 0 2540 12216 15621 Size: 2540 9676 3405 Fragments arranged by size: 9676 3405 2540 AleI CACnn'nnGTG Cuts at: 0 1722 4813 9986 10038 14935 15621 Size: 1722 3091 5173 52 4897 686 Fragments arranged by size: 5173 4897 3091 1722 686 52 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2144 4267 5043 8003 8951 9122 11610 11620 Size: 2144 2123 776 2960 948 171 2488 10 Cuts at: 11620 15621 Size: 4001 Fragments arranged by size: 4001 2960 2488 2144 2123 948 776 171 10 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 97 129 15621 Size: 97 32 15492 Fragments arranged by size: 15492 97 32 AlwNI CAG_nnn'CTG Cuts at: 0 100 162 350 1051 4260 4512 5565 5919 Size: 100 62 188 701 3209 252 1053 354 Cuts at: 5919 6454 7059 7533 7800 9162 11838 12068 12945 Size: 535 605 474 267 1362 2676 230 877 Cuts at: 12945 13800 15243 15270 15592 15621 Size: 855 1443 27 322 29 Fragments arranged by size: 3209 2676 1443 1362 1053 877 855 701 605 535 474 354 322 267 252 230 188 100 62 29 27 ApaI G_GGCC'C Cuts at: 0 181 8365 8664 15621 Size: 181 8184 299 6957 Fragments arranged by size: 8184 6957 299 181 ApaLI G'TGCA_C Cuts at: 0 3972 5745 7351 11440 12043 15621 Size: 3972 1773 1606 4089 603 3578 Fragments arranged by size: 4089 3972 3578 1773 1606 603 ApoI r'AATT_y Cuts at: 0 1219 3070 4137 6194 7291 8845 9992 12124 Size: 1219 1851 1067 2057 1097 1554 1147 2132 Cuts at: 12124 13204 13986 15515 15621 Size: 1080 782 1529 106 Fragments arranged by size: 2132 2057 1851 1554 1529 1219 1147 1097 1080 1067 782 106 AvaI C'yCGr_G Cuts at: 0 192 220 450 757 1122 2506 5160 5463 Size: 192 28 230 307 365 1384 2654 303 Cuts at: 5463 5866 6819 7716 9010 9716 10262 11413 11824 Size: 403 953 897 1294 706 546 1151 411 Cuts at: 11824 13036 13616 15621 Size: 1212 580 2005 Fragments arranged by size: 2654 2005 1384 1294 1212 1151 953 897 706 580 546 411 403 365 307 303 230 192 28 AvrII C'CTAG_G Cuts at: 0 3112 11802 15621 Size: 3112 8690 3819 Fragments arranged by size: 8690 3819 3112 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 6335 6368 13497 13530 15621 Size: 6335 33 7129 33 2091 Fragments arranged by size: 7129 6335 2091 33 33 BamHI G'GATC_C Cuts at: 0 7145 7238 12692 13830 15120 15621 Size: 7145 93 5454 1138 1290 501 Fragments arranged by size: 7145 5454 1290 1138 501 93 BanI G'GyrC_C Cuts at: 0 1794 2077 2779 4647 5261 5360 5859 6109 Size: 1794 283 702 1868 614 99 499 250 Cuts at: 6109 6574 7074 7318 9084 12821 12977 15621 Size: 465 500 244 1766 3737 156 2644 Fragments arranged by size: 3737 2644 1868 1794 1766 702 614 500 499 465 283 250 244 156 99 BanII G_rGCy'C Cuts at: 0 181 199 230 722 758 1123 1465 3013 Size: 181 18 31 492 36 365 342 1548 Cuts at: 3013 3643 3779 4199 4711 5147 5291 5656 6331 Size: 630 136 420 512 436 144 365 675 Cuts at: 6331 7184 7672 8365 8664 8729 9572 10023 11684 Size: 853 488 693 299 65 843 451 1661 Cuts at: 11684 11754 13391 15331 15621 Size: 70 1637 1940 290 Fragments arranged by size: 1940 1661 1637 1548 853 843 693 675 630 512 492 488 451 436 420 365 365 342 299 290 181 144 136 70 65 36 31 18 BbeI G_GCGC'C Cuts at: 0 2081 6113 15621 Size: 2081 4032 9508 Fragments arranged by size: 9508 4032 2081 BbsI GAAGACnn'nnnn_ Cuts at: 0 880 1016 1191 2697 3171 4564 4827 5771 Size: 880 136 175 1506 474 1393 263 944 Cuts at: 5771 5835 8247 8555 8578 8600 8615 9642 10451 Size: 64 2412 308 23 22 15 1027 809 Cuts at: 10451 11847 11866 11902 12559 13460 13725 15621 Size: 1396 19 36 657 901 265 1896 Fragments arranged by size: 2412 1896 1506 1396 1393 1027 944 901 880 809 657 474 308 265 263 175 136 64 36 23 22 19 15 BbvCI CC'TCA_GC Cuts at: 0 798 3556 5332 7538 10176 11965 14332 14437 Size: 798 2758 1776 2206 2638 1789 2367 105 Cuts at: 14437 15349 15621 Size: 912 272 Fragments arranged by size: 2758 2638 2367 2206 1789 1776 912 798 272 105 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 946 980 2326 2360 5454 5488 12697 12731 Size: 946 34 1346 34 3094 34 7209 34 Cuts at: 12731 15621 Size: 2890 Fragments arranged by size: 7209 3094 2890 1346 946 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1994 3046 10752 12811 15621 Size: 1994 1052 7706 2059 2810 Fragments arranged by size: 7706 2810 2059 1994 1052 BclI T'GATC_A Cuts at: 0 3095 7509 7841 9050 15171 15621 Size: 3095 4414 332 1209 6121 450 Fragments arranged by size: 6121 4414 3095 1209 450 332 BfrBI ATG'CAT Cuts at: 0 2048 2993 8603 15621 Size: 2048 945 5610 7018 Fragments arranged by size: 7018 5610 2048 945 BglI GCCn_nnn'nGGC Cuts at: 0 431 808 5533 5887 11590 12658 13423 14621 Size: 431 377 4725 354 5703 1068 765 1198 Cuts at: 14621 15621 Size: 1000 Fragments arranged by size: 5703 4725 1198 1068 1000 765 431 377 354 BglII A'GATC_T Cuts at: 0 6861 8379 9272 10986 15621 Size: 6861 1518 893 1714 4635 Fragments arranged by size: 6861 4635 1714 1518 893 BlpI GC'TnA_GC Cuts at: 0 827 1117 2251 3012 4855 5698 9808 12847 Size: 827 290 1134 761 1843 843 4110 3039 Cuts at: 12847 14911 15341 15621 Size: 2064 430 280 Fragments arranged by size: 4110 3039 2064 1843 1134 843 827 761 430 290 280 Bme1580I G_kGCm'C Cuts at: 0 181 457 3976 5666 5749 6435 7079 7355 Size: 181 276 3519 1690 83 686 644 276 Cuts at: 7355 8184 8365 8664 10855 11444 12047 14297 15621 Size: 829 181 299 2191 589 603 2250 1324 Fragments arranged by size: 3519 2250 2191 1690 1324 829 686 644 603 589 299 276 276 181 181 83 BmgBI CAC'GTC Cuts at: 0 3177 4907 6171 7920 11853 11892 13698 15621 Size: 3177 1730 1264 1749 3933 39 1806 1923 Fragments arranged by size: 3933 3177 1923 1806 1749 1730 1264 39 BmrI ACTGGGnnnn_n' Cuts at: 0 1656 1837 5808 6351 7019 8667 11624 11748 Size: 1656 181 3971 543 668 1648 2957 124 Cuts at: 11748 13060 13720 14037 14158 15621 Size: 1312 660 317 121 1463 Fragments arranged by size: 3971 2957 1656 1648 1463 1312 668 660 543 317 181 124 121 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 92 2190 14452 15497 15499 15621 Size: 92 2098 12262 1045 2 122 Fragments arranged by size: 12262 2098 1045 122 92 2 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 493 1504 2154 2185 2835 3337 4204 6577 Size: 493 1011 650 31 650 502 867 2373 Cuts at: 6577 7051 7368 9526 9559 9946 9948 10822 10825 Size: 474 317 2158 33 387 2 874 3 Cuts at: 10825 11176 11698 12116 12552 12553 12611 13510 13805 Size: 351 522 418 436 1 58 899 295 Cuts at: 13805 13891 14086 14239 14878 15621 Size: 86 195 153 639 743 Fragments arranged by size: 2373 2158 1011 899 874 867 743 650 650 639 522 502 493 474 436 418 387 351 317 295 195 153 86 58 33 31 3 2 1 Bpu10I CC'TnA_GC Cuts at: 0 798 1464 1984 3556 3637 5134 5146 5332 Size: 798 666 520 1572 81 1497 12 186 Cuts at: 5332 7297 7538 10176 10418 11965 14332 14437 15349 Size: 1965 241 2638 242 1547 2367 105 912 Cuts at: 15349 15621 Size: 272 Fragments arranged by size: 2638 2367 1965 1572 1547 1497 912 798 666 520 272 242 241 186 105 81 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1000 2425 3826 7141 12568 13451 14548 14902 Size: 1000 1425 1401 3315 5427 883 1097 354 Cuts at: 14902 15134 15621 Size: 232 487 Fragments arranged by size: 5427 3315 1425 1401 1097 1000 883 487 354 232 BsaI GGTCTCn'nnnn_ Cuts at: 0 934 5392 5447 10637 13903 14830 15621 Size: 934 4458 55 5190 3266 927 791 Fragments arranged by size: 5190 4458 3266 934 927 791 55 BsaAI yAC'GTr Cuts at: 0 1791 6003 6349 8704 9147 9384 11796 12042 Size: 1791 4212 346 2355 443 237 2412 246 Cuts at: 12042 15186 15621 Size: 3144 435 Fragments arranged by size: 4212 3144 2412 2355 1791 443 435 346 246 237 BsaBI GATnn'nnATC Cuts at: 0 13807 15621 Size: 13807 1814 BsaHI Gr'CG_yC Cuts at: 0 124 2078 6110 6310 7031 8351 9464 9647 Size: 124 1954 4032 200 721 1320 1113 183 Cuts at: 9647 9671 11401 15380 15621 Size: 24 1730 3979 241 Fragments arranged by size: 4032 3979 1954 1730 1320 1113 721 241 200 183 124 24 BsaWI w'CCGG_w Cuts at: 0 661 2273 6881 7229 7580 10334 15621 Size: 661 1612 4608 348 351 2754 5287 Fragments arranged by size: 5287 4608 2754 1612 661 351 348 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 97 127 472 502 709 739 894 924 Size: 97 30 345 30 207 30 155 30 Cuts at: 924 1124 1154 2503 2533 3424 3454 3625 3655 Size: 200 30 1349 30 891 30 171 30 Cuts at: 3655 4327 4357 6160 6190 6782 6812 9832 9862 Size: 672 30 1803 30 592 30 3020 30 Cuts at: 9862 10019 10049 11149 11179 12754 12784 15621 Size: 157 30 1100 30 1575 30 2837 Fragments arranged by size: 3020 2837 1803 1575 1349 1100 891 672 592 345 207 200 171 157 155 97 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 238 899 1145 1223 1226 1863 2524 3594 Size: 238 661 246 78 3 637 661 1070 Cuts at: 3594 4738 4762 5286 5629 5740 6168 6208 6339 Size: 1144 24 524 343 111 428 40 131 Cuts at: 6339 6453 7609 7663 8745 9580 9769 10471 10525 Size: 114 1156 54 1082 835 189 702 54 Cuts at: 10525 10678 11173 11228 11356 12274 14532 14839 15337 Size: 153 495 55 128 918 2258 307 498 Cuts at: 15337 15460 15621 Size: 123 161 Fragments arranged by size: 2258 1156 1144 1082 1070 918 835 702 661 661 637 524 498 495 428 343 307 246 238 189 161 153 131 128 123 114 111 78 55 54 54 40 24 3 BseYI C'CCAG_C Cuts at: 0 413 2073 2871 2942 3661 4286 4441 4885 Size: 413 1660 798 71 719 625 155 444 Cuts at: 4885 7665 9242 9822 10163 10422 11258 13413 13674 Size: 2780 1577 580 341 259 836 2155 261 Cuts at: 13674 13890 15010 15621 Size: 216 1120 611 Fragments arranged by size: 2780 2155 1660 1577 1120 836 798 719 625 611 580 444 413 341 261 259 216 155 71 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1064 1243 2077 4030 4426 4672 4861 4867 Size: 1064 179 834 1953 396 246 189 6 Cuts at: 4867 5190 5577 6205 7917 8445 8563 9895 10639 Size: 323 387 628 1712 528 118 1332 744 Cuts at: 10639 10644 11104 11364 11580 12895 14863 15262 15382 Size: 5 460 260 216 1315 1968 399 120 Cuts at: 15382 15621 Size: 239 Fragments arranged by size: 1968 1953 1712 1332 1315 1064 834 744 628 528 460 399 396 387 323 260 246 239 216 189 179 120 118 6 5 BsiEI CG_ry'CG Cuts at: 0 7658 12897 15621 Size: 7658 5239 2724 Fragments arranged by size: 7658 5239 2724 BsiHKAI G_wGCw'C Cuts at: 0 722 2339 3013 3643 3976 4199 5045 5093 Size: 722 1617 674 630 333 223 846 48 Cuts at: 5093 5140 5147 5291 5749 5807 6158 6331 7184 Size: 47 7 144 458 58 351 173 853 Cuts at: 7184 7355 7961 8729 9025 9572 10688 11444 11684 Size: 171 606 768 296 547 1116 756 240 Cuts at: 11684 11702 11795 11801 11852 11992 12047 15109 15621 Size: 18 93 6 51 140 55 3062 512 Fragments arranged by size: 3062 1617 1116 853 846 768 756 722 674 630 606 547 512 458 351 333 296 240 223 173 171 144 140 93 58 55 51 48 47 18 7 6 BsmI GAATG_Cn' Cuts at: 0 4459 6592 7992 11944 12481 13222 15621 Size: 4459 2133 1400 3952 537 741 2399 Fragments arranged by size: 4459 3952 2399 2133 1400 741 537 BsmBI CGTCTCn'nnnn_ Cuts at: 0 952 7022 7645 8517 10928 13688 14524 15621 Size: 952 6070 623 872 2411 2760 836 1097 Fragments arranged by size: 6070 2760 2411 1097 952 872 836 623 Bsp1286I G_dGCh'C Cuts at: 0 181 199 230 457 722 758 1123 1465 Size: 181 18 31 227 265 36 365 342 Cuts at: 1465 2339 3013 3643 3779 3976 4199 4711 5045 Size: 874 674 630 136 197 223 512 334 Cuts at: 5045 5093 5140 5147 5291 5656 5666 5749 5807 Size: 48 47 7 144 365 10 83 58 Cuts at: 5807 6158 6331 6435 7079 7184 7355 7672 7961 Size: 351 173 104 644 105 171 317 289 Cuts at: 7961 8184 8365 8664 8729 9025 9572 10023 10688 Size: 223 181 299 65 296 547 451 665 Cuts at: 10688 10855 11444 11684 11702 11754 11795 11801 11852 Size: 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723 583 449 429 415 364 363 233 116 102 90 19 BsrBI CCG'CTC Cuts at: 0 2161 12392 14644 14778 15621 Size: 2161 10231 2252 134 843 Fragments arranged by size: 10231 2252 2161 843 134 BsrDI GCAATG_nn' Cuts at: 0 1798 8933 10169 11594 13192 14205 15224 15621 Size: 1798 7135 1236 1425 1598 1013 1019 397 Fragments arranged by size: 7135 1798 1598 1425 1236 1019 1013 397 BsrFI r'CCGG_y Cuts at: 0 661 2390 5945 9500 13663 15621 Size: 661 1729 3555 3555 4163 1958 Fragments arranged by size: 4163 3555 3555 1958 1729 661 BsrGI T'GTAC_A Cuts at: 0 9470 12509 13145 14939 15621 Size: 9470 3039 636 1794 682 Fragments arranged by size: 9470 3039 1794 682 636 BssSI C'ACGA_G Cuts at: 0 4720 4730 7954 9127 13264 15621 Size: 4720 10 3224 1173 4137 2357 Fragments arranged by size: 4720 4137 3224 2357 1173 10 BstAPI GCAn_nnn'nTGC Cuts at: 0 59 817 1310 2247 2939 8586 9162 12051 Size: 59 758 493 937 692 5647 576 2889 Cuts at: 12051 12945 13580 14313 15621 Size: 894 635 733 1308 Fragments arranged by size: 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Bsu36I CC'TnA_GG Cuts at: 0 383 2144 3740 5212 6083 6436 7140 7912 Size: 383 1761 1596 1472 871 353 704 772 Cuts at: 7912 8950 11507 12061 12737 15621 Size: 1038 2557 554 676 2884 Fragments arranged by size: 2884 2557 1761 1596 1472 1038 871 772 704 676 554 383 353 BtgI C'CryG_G Cuts at: 0 376 2116 2843 3119 3977 4610 4916 5447 Size: 376 1740 727 276 858 633 306 531 Cuts at: 5447 6839 9797 10296 10517 10628 11265 11434 13421 Size: 1392 2958 499 221 111 637 169 1987 Cuts at: 13421 13535 14690 14726 15621 Size: 114 1155 36 895 Fragments arranged by size: 2958 1987 1740 1392 1155 895 858 727 637 633 531 499 376 306 276 221 169 114 111 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 7981 8103 8208 10477 13007 13244 15071 15621 Size: 7981 122 105 2269 2530 237 1827 550 Fragments arranged by size: 7981 2530 2269 1827 550 237 122 105 BtsI GCAGTG_nn' Cuts at: 0 924 2094 2921 4848 5805 6609 6990 8476 Size: 924 1170 827 1927 957 804 381 1486 Cuts at: 8476 9151 10578 12045 12775 15621 Size: 675 1427 1467 730 2846 Fragments arranged by size: 2846 1927 1486 1467 1427 1170 957 924 827 804 730 675 381 ClaI AT'CG_AT Cuts at: 0 9766 10325 15621 Size: 9766 559 5296 Fragments arranged by size: 9766 5296 559 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 486 521 15621 Size: 486 35 15100 Fragments arranged by size: 15100 486 35 DraI TTT'AAA Cuts at: 0 3143 10769 13985 15621 Size: 3143 7626 3216 1636 Fragments arranged by size: 7626 3216 3143 1636 DraIII CAC_nnn'GTG Cuts at: 0 8412 12828 14265 15621 Size: 8412 4416 1437 1356 Fragments arranged by size: 8412 4416 1437 1356 DrdI GACnn_nn'nnGTC Cuts at: 0 8767 15621 Size: 8767 6854 EaeI y'GGCC_r Cuts at: 0 432 551 2972 4607 4613 4659 5406 5481 Size: 432 119 2421 1635 6 46 747 75 Cuts at: 5481 5505 7524 7655 8060 8999 9372 10449 12755 Size: 24 2019 131 405 939 373 1077 2306 Cuts at: 12755 12894 13022 13337 13521 13665 13880 14141 15621 Size: 139 128 315 184 144 215 261 1480 Fragments arranged by size: 2421 2306 2019 1635 1480 1077 939 747 432 405 373 315 261 215 184 144 139 131 128 119 75 46 24 6 EagI C'GGCC_G Cuts at: 0 7655 12894 15621 Size: 7655 5239 2727 Fragments arranged by size: 7655 5239 2727 EarI CTCTTCn'nnn_ Cuts at: 0 1169 1753 2275 3709 5056 5279 6001 6336 Size: 1169 584 522 1434 1347 223 722 335 Cuts at: 6336 6703 6880 8299 8389 9449 9547 9616 10266 Size: 367 177 1419 90 1060 98 69 650 Cuts at: 10266 10775 11414 11788 12292 12523 12590 15542 15621 Size: 509 639 374 504 231 67 2952 79 Fragments arranged by size: 2952 1434 1419 1347 1169 1060 722 650 639 584 522 509 504 374 367 335 231 223 177 98 90 79 69 67 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 4524 8560 9427 10456 10961 12236 12685 15445 Size: 4524 4036 867 1029 505 1275 449 2760 Cuts at: 15445 15621 Size: 176 Fragments arranged by size: 4524 4036 2760 1275 1029 867 505 449 176 EcoICRI GAG'CTC Cuts at: 0 720 3011 3641 4197 5145 5289 6329 7182 Size: 720 2291 630 556 948 144 1040 853 Cuts at: 7182 8727 9570 11682 15621 Size: 1545 843 2112 3939 Fragments arranged by size: 3939 2291 2112 1545 1040 948 853 843 720 630 556 144 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 184 493 722 992 1028 1504 1596 2154 Size: 184 309 229 270 36 476 92 558 Cuts at: 2154 2185 2206 2759 2835 3025 3165 3337 3502 Size: 31 21 553 76 190 140 172 165 Cuts at: 3502 4098 4204 4342 4437 4576 5811 6317 6577 Size: 596 106 138 95 139 1235 506 260 Cuts at: 6577 7051 7368 8302 8416 8590 8943 9526 9559 Size: 474 317 934 114 174 353 583 33 Cuts at: 9559 9883 9946 9948 10822 10825 11176 11275 11644 Size: 324 63 2 874 3 351 99 369 Cuts at: 11644 11698 12116 12512 12552 12553 12611 13090 13472 Size: 54 418 396 40 1 58 479 382 Cuts at: 13472 13510 13805 13882 13891 14086 14239 14878 15621 Size: 38 295 77 9 195 153 639 743 Fragments arranged by size: 1235 934 874 743 639 596 583 558 553 506 479 476 474 418 396 382 369 353 351 324 317 309 295 270 260 229 195 190 184 174 172 165 153 140 139 138 114 106 99 95 92 77 76 63 58 54 40 38 36 33 31 21 9 3 2 1 EcoNI CCTnn'n_nnAGG Cuts at: 0 649 1567 3111 4048 4270 7051 8398 8770 Size: 649 918 1544 937 222 2781 1347 372 Cuts at: 8770 11382 11725 12595 12679 12740 14317 14370 15621 Size: 2612 343 870 84 61 1577 53 1251 Fragments arranged by size: 2781 2612 1577 1544 1347 1251 937 918 870 649 372 343 222 84 61 53 EcoO109I rG'GnC_Cy Cuts at: 0 177 794 1560 2714 5228 5904 6147 7044 Size: 177 617 766 1154 2514 676 243 897 Cuts at: 7044 7056 8361 8496 8661 9401 9712 12015 12065 Size: 12 1305 135 165 740 311 2303 50 Cuts at: 12065 12879 13297 13866 14622 14781 15621 Size: 814 418 569 756 159 840 Fragments arranged by size: 2514 2303 1305 1154 897 840 814 766 756 740 676 617 569 418 311 243 177 165 159 135 50 12 EcoRI G'AATT_C Cuts at: 0 8845 12124 15621 Size: 8845 3279 3497 Fragments arranged by size: 8845 3497 3279 EcoRV GAT'ATC Cuts at: 0 9525 10919 13242 15621 Size: 9525 1394 2323 2379 Fragments arranged by size: 9525 2379 2323 1394 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2734 3195 3690 4092 8655 8865 12321 12441 Size: 2734 461 495 402 4563 210 3456 120 Cuts at: 12441 15621 Size: 3180 Fragments arranged by size: 4563 3456 3180 2734 495 461 402 210 120 FspI TGC'GCA Cuts at: 0 6134 11926 13939 15621 Size: 6134 5792 2013 1682 Fragments arranged by size: 6134 5792 2013 1682 FspAI rTGC'GCAy Cuts at: 0 11926 15621 Size: 11926 3695 HaeII r_GCGC'y Cuts at: 0 2081 6113 6340 6871 7929 8741 8835 9815 Size: 2081 4032 227 531 1058 812 94 980 Cuts at: 9815 9933 10163 10628 11413 12306 15453 15621 Size: 118 230 465 785 893 3147 168 Fragments arranged by size: 4032 3147 2081 1058 980 893 812 785 531 465 230 227 168 118 94 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 472 504 1942 1974 2503 2526 2535 2558 Size: 472 32 1438 32 529 23 9 23 Cuts at: 2558 3865 3897 3989 4021 4327 4359 4864 4896 Size: 1307 32 92 32 306 32 505 32 Cuts at: 4896 6066 6098 6160 6192 8024 8056 9097 9129 Size: 1170 32 62 32 1832 32 1041 32 Cuts at: 9129 9696 9728 9748 9780 9832 9864 10331 10363 Size: 567 32 20 32 52 32 467 32 Cuts at: 10363 11913 11945 12112 12144 12202 12234 12712 12744 Size: 1550 32 167 32 58 32 478 32 Cuts at: 12744 14282 14314 15621 Size: 1538 32 1307 Fragments arranged by size: 1832 1550 1538 1438 1307 1307 1170 1041 567 529 505 478 472 467 306 167 92 62 58 52 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 23 23 20 9 HincII GTy'rAC Cuts at: 0 1428 3266 7089 11336 13118 14006 15621 Size: 1428 1838 3823 4247 1782 888 1615 Fragments arranged by size: 4247 3823 1838 1782 1615 1428 888 HindIII A'AGCT_T Cuts at: 0 8981 15621 Size: 8981 6640 HpaI GTT'AAC Cuts at: 0 13118 15621 Size: 13118 2503 KasI G'GCGC_C Cuts at: 0 2077 6109 15621 Size: 2077 4032 9512 Fragments arranged by size: 9512 4032 2077 KpnI G_GTAC'C Cuts at: 0 1798 5265 7322 12981 15621 Size: 1798 3467 2057 5659 2640 Fragments arranged by size: 5659 3467 2640 2057 1798 MluI A'CGCG_T Cuts at: 0 10715 15621 Size: 10715 4906 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 3288 5967 6878 7418 8678 11141 11518 11743 Size: 3288 2679 911 540 1260 2463 377 225 Cuts at: 11743 13465 14828 15621 Size: 1722 1363 793 Fragments arranged by size: 3288 2679 2463 1722 1363 1260 911 793 540 377 225 MscI TGG'CCA Cuts at: 0 434 553 2974 4609 4661 5408 5507 7526 Size: 434 119 2421 1635 52 747 99 2019 Cuts at: 7526 8062 9001 12757 13024 13339 13882 14143 15621 Size: 536 939 3756 267 315 543 261 1478 Fragments arranged by size: 3756 2421 2019 1635 1478 939 747 543 536 434 315 267 261 119 99 52 MslI CAynn'nnrTG Cuts at: 0 1722 2126 3586 4372 4636 4813 6940 7219 Size: 1722 404 1460 786 264 177 2127 279 Cuts at: 7219 8068 8695 8881 9986 10038 10325 10348 11060 Size: 849 627 186 1105 52 287 23 712 Cuts at: 11060 11080 11329 11602 13918 14935 15621 Size: 20 249 273 2316 1017 686 Fragments arranged by size: 2316 2127 1722 1460 1105 1017 849 786 712 686 627 404 287 279 273 264 249 186 177 52 23 20 MspA1I CmG'CkG Cuts at: 0 1048 1433 1588 1722 2118 2845 3121 3729 Size: 1048 385 155 134 396 727 276 608 Cuts at: 3729 4354 5057 5124 5295 5639 5697 7621 7689 Size: 625 703 67 171 344 58 1924 68 Cuts at: 7689 7983 8586 9048 9838 10395 11135 11676 11714 Size: 294 603 462 790 557 740 541 38 Cuts at: 11714 12136 12858 13689 14047 14469 14871 15042 15112 Size: 422 722 831 358 422 402 171 70 Cuts at: 15112 15135 15267 15566 15589 15621 Size: 23 132 299 23 32 Fragments arranged by size: 1924 1048 831 790 740 727 722 703 625 608 603 557 541 462 422 422 402 396 385 358 344 299 294 276 171 171 155 134 132 70 68 67 58 38 32 23 23 NarI GG'CG_CC Cuts at: 0 2078 6110 15621 Size: 2078 4032 9511 Fragments arranged by size: 9511 4032 2078 NcoI C'CATG_G Cuts at: 0 376 4916 6839 10517 11265 13421 15621 Size: 376 4540 1923 3678 748 2156 2200 Fragments arranged by size: 4540 3678 2200 2156 1923 748 376 NdeI CA'TA_TG Cuts at: 0 7514 15621 Size: 7514 8107 NsiI A_TGCA'T Cuts at: 0 2050 2995 8605 15621 Size: 2050 945 5610 7016 Fragments arranged by size: 7016 5610 2050 945 NspI r_CATG'y Cuts at: 0 25 2766 5547 10381 10638 11706 12175 12516 Size: 25 2741 2781 4834 257 1068 469 341 Cuts at: 12516 13432 13709 14758 15132 15498 15621 Size: 916 277 1049 374 366 123 Fragments arranged by size: 4834 2781 2741 1068 1049 916 469 374 366 341 277 257 123 25 PasI CC'CwG_GG Cuts at: 0 5649 5658 10976 15621 Size: 5649 9 5318 4645 Fragments arranged by size: 5649 5318 4645 9 PciI A'CATG_T Cuts at: 0 13705 15621 Size: 13705 1916 PflMI CCAn_nnn'nTGG Cuts at: 0 534 3657 5415 7533 7800 8985 11550 13674 Size: 534 3123 1758 2118 267 1185 2565 2124 Cuts at: 13674 14303 15621 Size: 629 1318 Fragments arranged by size: 3123 2565 2124 2118 1758 1318 1185 629 534 267 PfoI T'CCnGG_A Cuts at: 0 244 587 1094 2189 2363 2397 2480 4220 Size: 244 343 507 1095 174 34 83 1740 Cuts at: 4220 4292 6125 6296 8771 10586 10841 15621 Size: 72 1833 171 2475 1815 255 4780 Fragments arranged by size: 4780 2475 1833 1815 1740 1095 507 343 255 244 174 171 83 72 34 PmlI CAC'GTG Cuts at: 0 1791 6349 8704 11796 12042 15186 15621 Size: 1791 4558 2355 3092 246 3144 435 Fragments arranged by size: 4558 3144 3092 2355 1791 435 246 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 97 129 1372 1404 7210 7242 15621 Size: 97 32 1243 32 5806 32 8379 Fragments arranged by size: 8379 5806 1243 97 32 32 32 PpuMI rG'GwC_Cy Cuts at: 0 1560 2714 5904 6147 7044 8496 9401 9712 Size: 1560 1154 3190 243 897 1452 905 311 Cuts at: 9712 12015 13866 14781 15621 Size: 2303 1851 915 840 Fragments arranged by size: 3190 2303 1851 1560 1452 1154 915 905 897 840 311 243 PshAI GACnn'nnGTC Cuts at: 0 1277 9392 11870 12382 15621 Size: 1277 8115 2478 512 3239 Fragments arranged by size: 8115 3239 2478 1277 512 PsiI TTA'TAA Cuts at: 0 1364 3328 10195 15621 Size: 1364 1964 6867 5426 Fragments arranged by size: 6867 5426 1964 1364 PspOMI G'GGCC_C Cuts at: 0 177 8361 8660 15621 Size: 177 8184 299 6961 Fragments arranged by size: 8184 6961 299 177 PspXI vC'TCGA_Gb Cuts at: 0 5866 13036 15621 Size: 5866 7170 2585 Fragments arranged by size: 7170 5866 2585 PstI C_TGCA'G Cuts at: 0 55 84 524 773 1625 2569 2629 2664 Size: 55 29 440 249 852 944 60 35 Cuts at: 2664 4256 4843 5032 5477 6397 6481 6968 7371 Size: 1592 587 189 445 920 84 487 403 Cuts at: 7371 8504 8898 9158 9167 9253 9317 9695 10049 Size: 1133 394 260 9 86 64 378 354 Cuts at: 10049 10376 11363 11477 12720 12782 13353 13850 14230 Size: 327 987 114 1243 62 571 497 380 Cuts at: 14230 14318 14569 15047 15621 Size: 88 251 478 574 Fragments arranged by size: 1592 1243 1133 987 944 920 852 587 574 571 497 487 478 445 440 403 394 380 378 354 327 260 251 249 189 114 88 86 84 64 62 60 55 35 29 9 PvuII CAG'CTG Cuts at: 0 1048 1722 5057 5124 5697 7621 7689 7983 Size: 1048 674 3335 67 573 1924 68 294 Cuts at: 7983 9048 10395 11135 12136 14047 14469 15042 15112 Size: 1065 1347 740 1001 1911 422 573 70 Cuts at: 15112 15135 15267 15566 15589 15621 Size: 23 132 299 23 32 Fragments arranged by size: 3335 1924 1911 1347 1065 1048 1001 740 674 573 573 422 299 294 132 70 68 67 32 23 23 SacI G_AGCT'C Cuts at: 0 722 3013 3643 4199 5147 5291 6331 7184 Size: 722 2291 630 556 948 144 1040 853 Cuts at: 7184 8729 9572 11684 15621 Size: 1545 843 2112 3937 Fragments arranged by size: 3937 2291 2112 1545 1040 948 853 843 722 630 556 144 SacII CC_GC'GG Cuts at: 0 2119 2846 3122 15621 Size: 2119 727 276 12499 Fragments arranged by size: 12499 2119 727 276 SalI G'TCGA_C Cuts at: 0 7087 15621 Size: 7087 8534 SanDI GG'GwC_CC Cuts at: 0 2714 5904 9401 9712 15621 Size: 2714 3190 3497 311 5909 Fragments arranged by size: 5909 3497 3190 2714 311 SapI GCTCTTCn'nnn_ Cuts at: 0 3709 5279 6336 9547 12523 15621 Size: 3709 1570 1057 3211 2976 3098 Fragments arranged by size: 3709 3211 3098 2976 1570 1057 SbfI CC_TGCA'GG Cuts at: 0 524 6397 15621 Size: 524 5873 9224 Fragments arranged by size: 9224 5873 524 ScaI AGT'ACT Cuts at: 0 1483 4738 9355 9859 10066 15621 Size: 1483 3255 4617 504 207 5555 Fragments arranged by size: 5555 4617 3255 1483 504 207 SexAI A'CCwGG_T Cuts at: 0 1562 2024 2306 4973 8408 12212 12833 12980 Size: 1562 462 282 2667 3435 3804 621 147 Cuts at: 12980 14816 15621 Size: 1836 805 Fragments arranged by size: 3804 3435 2667 1836 1562 805 621 462 282 147 SfcI C'TryA_G Cuts at: 0 51 80 520 769 821 1621 2565 2625 Size: 51 29 440 249 52 800 944 60 Cuts at: 2625 2660 4252 4839 5028 5473 5673 6393 6477 Size: 35 1592 587 189 445 200 720 84 Cuts at: 6477 6964 7367 7632 8500 8868 8894 9154 9163 Size: 487 403 265 868 368 26 260 9 Cuts at: 9163 9249 9313 9691 10045 10372 11359 11473 11647 Size: 86 64 378 354 327 987 114 174 Cuts at: 11647 12716 12778 13349 13846 14226 14314 14565 15043 Size: 1069 62 571 497 380 88 251 478 Cuts at: 15043 15051 15621 Size: 8 570 Fragments arranged by size: 1592 1069 987 944 868 800 720 587 571 570 497 487 478 445 440 403 380 378 368 354 327 265 260 251 249 200 189 174 114 88 86 84 64 62 60 52 51 35 29 26 9 8 SfiI GGCCn_nnn'nGGCC Cuts at: 0 431 15621 Size: 431 15190 SfoI GGC'GCC Cuts at: 0 2079 6111 15621 Size: 2079 4032 9510 Fragments arranged by size: 9510 4032 2079 SmaI CCC'GGG Cuts at: 0 194 452 759 1124 6821 9718 10264 15621 Size: 194 258 307 365 5697 2897 546 5357 Fragments arranged by size: 5697 5357 2897 546 365 307 258 194 SmlI C'TyrA_G Cuts at: 0 1015 2404 2584 3805 3961 5223 5866 7120 Size: 1015 1389 180 1221 156 1262 643 1254 Cuts at: 7120 12306 12547 12613 13036 13466 14527 14917 15149 Size: 5186 241 66 423 430 1061 390 232 Cuts at: 15149 15621 Size: 472 Fragments arranged by size: 5186 1389 1262 1254 1221 1061 1015 643 472 430 423 390 241 232 180 156 66 SphI G_CATG'C Cuts at: 0 10638 12175 13432 15621 Size: 10638 1537 1257 2189 Fragments arranged by size: 10638 2189 1537 1257 SspI AAT'ATT Cuts at: 0 12457 15621 Size: 12457 3164 StuI AGG'CCT Cuts at: 0 644 1868 2515 4864 6399 6691 12661 13075 Size: 644 1224 647 2349 1535 292 5970 414 Cuts at: 13075 15196 15621 Size: 2121 425 Fragments arranged by size: 5970 2349 2121 1535 1224 647 644 425 414 292 StyI C'CwwG_G Cuts at: 0 29 376 950 1995 2709 3112 4067 4916 Size: 29 347 574 1045 714 403 955 849 Cuts at: 4916 5403 6839 8741 8784 9224 10517 10884 11265 Size: 487 1436 1902 43 440 1293 367 381 Cuts at: 11265 11802 12758 12920 13421 14705 15621 Size: 537 956 162 501 1284 916 Fragments arranged by size: 1902 1436 1293 1284 1045 956 955 916 849 714 574 537 501 487 440 403 381 367 347 162 43 29 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1675 1744 4028 4665 5425 6189 6923 8571 Size: 1675 69 2284 637 760 764 734 1648 Cuts at: 8571 8792 9256 9382 9570 11427 11860 12900 12974 Size: 221 464 126 188 1857 433 1040 74 Cuts at: 12974 13727 15006 15157 15621 Size: 753 1279 151 464 Fragments arranged by size: 2284 1857 1675 1648 1279 1040 764 760 753 734 637 464 464 433 221 188 151 126 74 69 TatI w'GTAC_w Cuts at: 0 1481 1739 4736 9236 9353 9470 9857 10064 Size: 1481 258 2997 4500 117 117 387 207 Cuts at: 10064 11180 12419 12509 13145 14939 15621 Size: 1116 1239 90 636 1794 682 Fragments arranged by size: 4500 2997 1794 1481 1239 1116 682 636 387 258 207 117 117 90 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1124 1156 1915 1947 3625 3657 4512 4544 Size: 1124 32 759 32 1678 32 855 32 Cuts at: 4544 6185 6217 6782 6814 8398 8430 8930 8946 Size: 1641 32 565 32 1584 32 500 16 Cuts at: 8946 8962 8978 9143 9175 9599 9631 10702 10734 Size: 16 16 165 32 424 32 1071 32 Cuts at: 10734 11149 11181 12754 12786 15621 Size: 415 32 1573 32 2835 Fragments arranged by size: 2835 1678 1641 1584 1573 1124 1071 855 759 565 500 424 415 165 32 32 32 32 32 32 32 32 32 32 32 32 16 16 16 Tth111I GACn'n_nGTC Cuts at: 0 5919 8779 12019 12928 15621 Size: 5919 2860 3240 909 2693 Fragments arranged by size: 5919 3240 2860 2693 909 XbaI T'CTAG_A Cuts at: 0 14073 15621 Size: 14073 1548 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 468 3658 4960 8340 9144 11214 11551 13591 Size: 468 3190 1302 3380 804 2070 337 2040 Cuts at: 13591 15621 Size: 2030 Fragments arranged by size: 3380 3190 2070 2040 2030 1302 804 468 337 XhoI C'TCGA_G Cuts at: 0 5866 13036 15621 Size: 5866 7170 2585 Fragments arranged by size: 7170 5866 2585 XmaI C'CCGG_G Cuts at: 0 192 450 757 1122 6819 9716 10262 15621 Size: 192 258 307 365 5697 2897 546 5359 Fragments arranged by size: 5697 5359 2897 546 365 307 258 192 XmnI GAAnn'nnTTC Cuts at: 0 1651 2688 7939 8254 8919 11288 11308 12481 Size: 1651 1037 5251 315 665 2369 20 1173 Cuts at: 12481 14389 15518 15621 Size: 1908 1129 103 Fragments arranged by size: 5251 2369 1908 1651 1173 1129 1037 665 315 103 20 ZraI GAC'GTC Cuts at: 0 6311 7032 9465 9648 15621 Size: 6311 721 2433 183 5973 Fragments arranged by size: 6311 5973 2433 721 183 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NsiI NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SmaI SmlI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AseI AsiSI BmtI BsiWI BssHII BstBI FseI MfeI NaeI NgoMIV NheI NotI NruI PacI PmeI PsrI PvuI RsrII SgfI SgrAI SnaBI SpeI SrfI SwaI