################################## # NO CUT ENZYMES ( 63 enzymes) # ################################## Acc65I AclI AflII AgeI AscI AseI AsnI Asp718I AviII AvrII BbeI BcgI BciVI BfrI BlnI BsiWI Bsp1407I BspDI BspLU11I BsrGI BssHII BssSI BstBI ClaI CpoI EcoRI EcoRV EcoT22I FseI FspI HpaI KasI KpnI MfeI MluI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI SbfI ScaI SfoI SfuI SpeI Sse8387I SspBI SspI SwaI Van91I ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AatII 314 GACGTC GACGT^C AccIII 4085 TCCGGA T^CCGGA AflIII 3995 ACGTGT A^C[GA][CT]GT AhdI 4105 GACACGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 3879 GTGCAC G^TGCAC Aor51HI 3824 AGCGCT AGC^GCT ApaLI 3879 GTGCAC G^TGCAC AspEI 4105 GACACGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbrPI 3994 CACGTG CAC^GTG BseAI 4085 TCCGGA T^CCGGA BsiEI 4392 CGGCCG CG[GA][CT]^CG BspEI 4085 TCCGGA T^CCGGA BspHI 1028 TCATGA T^CATGA BsrDI 4730 GCAATG GCAATG(2/0) Bst1107I 4368 GTATAC GTA^TAC BstEII 1249 GGTCACC G^GT[ACGT]ACC BstPI 1249 GGTCACC G^GT[ACGT]ACC BstZ17I 4368 GTATAC GTA^TAC DraI 255 TTTAAA TTT^AAA DrdI 731 GACTCCGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 4392 CGGCCG C^GGCCG Eam1105I 4105 GACACGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 4392 CGGCCG C^GGCCG Eco47III 3824 AGCGCT AGC^GCT Eco52I 4392 CGGCCG C^GGCCG EcoO65I 1249 GGTCACC G^GT[ACGT]ACC MroI 4085 TCCGGA T^CCGGA PmaCI 3994 CACGTG CAC^GTG PmlI 3994 CACGTG CAC^GTG RcaI 1028 TCATGA T^CATGA SacI 4071 GAGCTC GAGCT^C SexAI 4063 ACCAGGT A^CC[AT]GGT SfiI 39 GGCCGTCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 1948 CGCCGGCG C[GA]^CCGG[CT]G SnaBI 203 TACGTA TAC^GTA XbaI 318 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 1870 GTCTAC GT^[AC][GT]AC 4368 GTATAC ApaI 519 GGGCCC GGGCC^C 4409 GGGCCC BamHI 1467 GGATCC G^GATCC 2547 GGATCC BbvCI 3753 CCTCAGC CCTCAGC(-5/-2) 4811 CCTCAGC BglI 40 GCCGTCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2689 GCCCGTCTGGC BlpI 439 GCTAAGC GC^T[ACGT]AGC 4020 GCTGAGC Bpu1102I 439 GCTAAGC GC^T[ACGT]AGC 4020 GCTGAGC BsaAI 203 TACGTA [CT]AC^GT[GA] 3994 CACGTG BsaBI 1515 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3393 GATAGAGATC BsaI 1594 GAGACC GGTCTC(1/5) 3604 GAGACC BsaWI 2918 ACCGGA [AT]^CCGG[AT] 4085 TCCGGA BsmI 1058 GCATTC GAATGC(1/-1) 1815 GCATTC BsrBI 4662 CCGCTC CCGCTC(-3/-3) 2062 GAGCGG BstAPI 1058 GCATTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4445 GCAAAGCCTGC CelII 439 GCTAAGC GC^T[ACGT]AGC 4020 GCTGAGC DraIII 3486 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG 4633 CACCGTGTG EcoNI 2559 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4119 CCTGCCGGAGG HincII 863 GTCAAC GT[CT]^[GA]AC 3052 GTCAAC HindII 863 GTCAAC GT[CT]^[GA]AC 3052 GTCAAC HindIII 3282 AAGCTT A^AGCTT 4777 AAGCTT KspI 3618 CCGCGG CCGC^GG 3950 CCGCGG MamI 1515 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3393 GATAGAGATC PvuII 2596 CAGCTG CAG^CTG 4018 CAGCTG SacII 3618 CCGCGG CCGC^GG 3950 CCGCGG SapI 3009 GAAGAGC GCTCTTC(1/4) 4692 GCTCTTC SmaI 23 CCCGGG CCC^GGG 4007 CCCGGG SphI 1739 GCATGC GCATG^C 4271 GCATGC XcmI 1856 CCACCACCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4294 CCAGGGTCAGACTGG XmaCI 23 CCCGGG C^CCGGG 4007 CCCGGG XmaI 23 CCCGGG C^CCGGG 4007 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 129 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApoI Asp700I AspHI AspI AvaI AvaII BalI BanI BanII BbsI BbvI BclI BcnI BfaI BglII BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PaeR7I PflFI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XhoI XhoII XmnI